Formula and method for cultivating Thalassiosa algae in water tanks for the production of unsaturated fatty acids.

Abstract

OCR 09WP 23 / 02 / 2569 Recipe and cultivation methods for Thalassiosaracira algae in water tanks for the production of non-fatty acids. Saturated, consisting of sodium chloride, sodium nitrate, sodium dihydrogen phosphate monohydrate, sodium melanin. Tasilicate nanohydrate, ferric chloride hexahydrate, ethylenediamine tetraacetic acid, zinc sulfate. Heptahydrate, cobalt sulfate heptahydrate, manganese chloride tetrahydrate, sodium molybdate dihydrate Sodium selenite, nickel chloride hexahydrate, vitamin B1, biotin, vitamin B12—each containing a different substance. Dissolve in filtered water: sodium chloride, sodium nitrate, sodium dihydrogen phosphate monohydrate. Sodium metasilicate nanohydrate, ferric chloride hexahydrate, and ethylenediaminetetraacetic acid. Dissolve acid in filtered water, mix until homogeneous, and adjust the volume to obtain a metal solution. Type 1: Cobalt sulfate heptahydrate, zinc sulfate heptahydrate, manganese chloride tetrahydrate. Sodium molybdate dihydrate, sodium molybdate dihydrate, sodium selenite, nickel chloride hexahydrate Dissolve in filtered water, mix until homogeneous, and adjust the volume to obtain a second type of metal solution. Dissolve vitamin B1, biotin, and vitamin B12 in filtered water and mix until homogeneous. Adjust the volume to obtain a vitamin solution. Mix all the solutions together, adjust the volume with filtered water, and then use it. Cultivation of Thalassiosira algae.

Claims

OCR 09WP 23 / 02 / 2569 1. A formula for culturing Thalassiosaracira algae in water tanks for the production of unsaturated fatty acids. together with Sodium chloride 6.35-14.83 grams per liter. Sodium nitrate 14.00-32.66 mg / L Sodium dihydrogen phosphate monohydrate 0.92-2.16 g / L Sodium metasilicate nanohydrate 4.5-10.5 g / L Ferric chloride hexahydrate 0.53-1.23 g / L Ethylenediaminetetraacetic acid 0.92-2.16 g / L Zinc sulfate heptahydrate 0.021-0.051 grams per liter. Cobalt sulfate heptahydrate 0.004-0.011 grams per liter. Manganese chloride tetrahydrate 0.16-0.37 g / L Sodium molybdate dihydrate 0.44-1.03 mg / L Sodium selenite 0.05-0.12 mg / L Nickel chloride hexahydrate 0.447-1.04 mg / L Vitamin B1: 30-70 milligrams per liter. Biotin 0.03-0.07 mg / L Vitamin B12: 0.6-1.4 mg / L 2. Method for cultivating Thalassiosa algae in water tanks for the production of unsaturated fatty acids. Under Claim 1, the following steps apply: A. Dissolve the sodium chloride in 500 ml of filtered water until it is homogeneous. B. Dissolve the sodium nitrate in 100 ml of filtered water until it is homogeneous. C. Dissolve sodium dihydrogen phosphate monohydrate in 100 ml of filtered water. milliliters until homogeneous. d. Dissolve the sodium metasilicate nanohydrate in 100 ml of filtered water until... Homogeneous The province introduced ferric chloride hexahydrate and ethylenediaminetetraacetic acid. Dissolve in filtered water, mix until homogeneous, then adjust the volume to a total of 1,000 milliliters. A type 1 metal solution was obtained, and 1 milliliter of the solution was taken out. F. Add cobalt sulfate heptahydrate, zinc sulfate heptahydrate, manganese chloride. Hydrated sodium molybdate, sodium selenite, nickel chloride hexahydrate, dissolved in... Filter the water, mix until homogeneous, then adjust the volume to a total of 1,000 milliliters. This will yield... Metal solution type 2 B. Dissolve vitamin B1, biotin, and vitamin B12 in filtered water and mix until... Mix thoroughly and adjust the volume to a total of 1,000 milliliters to obtain a vitamin solution. C. Mix the solutions obtained from steps A - E together. J. Add 1 ml each of the solutions set aside from steps E, F, and G to step H. Adjust. The total volume of food containing filtered water should be 1,000 milliliters. F. Cultivate Thalassiosira algae at a temperature of 33 ± 2 degrees Celsius to maintain a high concentration. Light intensity of 40-60 micromol photons per square meter per second, and air supply of 2-4 liters of air per liter of food. Cultivate for 7 days.