Optimal cultivation methods for the growth and carotenoid production of the red yeast (Rhodotorula paludigena).

Abstract

This invention relates to a method for cultivating the red yeast rice, Rhodotorula paludigena. To create a suitable environment for growth and carotenoid production, the process is as follows: Red yeast is cultured in... The lab is shaken to stimulate growth, and then cultured in a bioreactor with a high rate of stirring. 600 rpm, air flow rate of 1 liter per minute, pH 6.0 for 32 to 36 hours. With the culture medium, adjust the cultivation conditions at a temperature of 22 to 30 degrees Celsius to obtain the desired process. Optimal cultivation conditions for the growth and carotenoid production of the red yeast Rhodotorula. Furthermore, the use of a developed nutrient formula in combination with cultivation in a bioreactor was also employed. Batch culture process, which involves improving the development of nutrient formulas and culture conditions. Greater efficiency, easier to scale up production, and suitable for further development in the field. The next industry

Claims

OCR 10KL (19 / 12 / 2568) 1. Methods for cultivating the red yeast (Rhodotorula paludigena) to optimize its growth and The production of carotenoids involves the following steps: A. Preparing starter cultures involves the following steps: - Transfer the yeast suspension onto YPD (Yeast extract Peptone) solid medium. Dextrose) is incubated at 28-30 degrees Celsius for 36-48 hours. - Starter culture preparation step 1: Transfer one loop of culture from the solid medium onto a flask. The liquid YPD medium is incubated on a shaker at 250 revolutions per minute at a temperature of 28-30°C. The fermented liquid was left at degrees Celsius for 20-24 hours. The absorbance of light at different wavelengths was then measured. A 600 nanometer wave adjusts the cell's turbidity to 6 (OD600 = 6). - The second stage of starter culture preparation involves adding 10% by volume of the first stage starter culture to... Flasks containing synthetic food composed of glucose and potassium dihydrogen. Phosphate, disodium hydrogen phosphate, magnesium sulfate heptahydrate, yeast extract. Ammonium sulfate, ferric chloride, calcium chloride dihydrate, and zinc sulfate heptahydrate. The hydrated material is incubated on a shaker at a speed of 250 revolutions per minute at a temperature of 28-30 degrees Celsius. After 20-24 hours, adjust the cell turbidity of the resulting fermented liquid to 12. (OD600 = 12) The resulting cell suspension will be used as a starter culture for cultivation in the reactor. Next biological step. B. Cultivation in a bioreactor consists of the following steps: - Assemble the bioreactor and calibrate the electrodes, then add the synthetic food. pH 6.0 containing glucose, potassium dihydrogen phosphate, and disodium. Hydrogen phosphate and magnesium sulfate heptahydrate. - Sterilize at 121 degrees Celsius for 30 minutes. Wait until the tank temperature reaches... The bioreactor is lowered; connect the bioreactor's signal cables to the control unit. Then add ammonium sulfate, calcium chloride dihydrate, zinc sulfate heptahydrate. Sterile ferric chloride was prepared with the selective addition of ammonium sulfate at a concentration of 3,5. Or 7 grams per liter, and ferric chloride at a concentration of 0.042 or 0.084 grams per liter. - Control the cultivation conditions in the bioreactor at a stirring rate of 600 revolutions per minute. Aeration rate: 1 liter per liter per minute. pH: 6.0, adjusted using a sodium solution. 2 molar hydroxide and 2 molar phosphoric acid solution, at a temperature of 22 degrees Celsius. Up to 30 degrees Celsius - Next, add 10% by volume of the starter culture from step A, determining the turbidity value. Start with 0.6 m³ of cells and cultivate them in a bioreactor under controlled conditions for a certain period of time. 32-36 hours is considered the end of the batch cultivation process. C. Harvesting red yeast cells. - Centrifugate the fermented liquid obtained from step B at a speed of 5,000-7,000 revolutions per minute. For 5-7 minutes, pour off the clear liquid and rinse the cell precipitate with a phosphoric acid solution. A concentration of 1 percent (weight / volume) and distilled water were used, respectively. The cell precipitate was then applied. Freeze dry for 24 hours, or until the cell sediment is completely dry.