The process of preparing microorganisms to reduce the release of ammonia that occurs during the organic fertilizer composting process.

Abstract

OCR 10KL (02 / 03 / 2569) The process of preparing microorganisms to reduce the release of ammonia that occurs during the composting process. The organic method involves preparing a starter culture of B. subtilis subsp. Inaquosorum that is capable of using ammonia. Under conditions with high levels of organic nitrogen, selected and purified strains are cultured in... Sterilized liquid culture medium and microbial inoculum mixture with a support material, incorporating the microorganism B. subtilis. Subsp. Inaquosorum was mixed together with a sterilized support material, which consisted of... Talcum powder and carboxyl methyl cellulose are mixed thoroughly. The product is uniformly processed, dehumidified, and then finely ground into a powder. This resulting product helps to solve the problem of ammonia odor. During the process of composting chicken manure, environmental problems are reduced.

Claims

OCR 10KL (14 / 07 / 2569) 1. The process of preparing microorganisms to reduce the release of ammonia that occurs during the organic fertilizer composting process. It consists of steps involving the preparation of starter cultures and the mixing of microorganisms with the substrate, with the following specific characteristics: A. Preparation of starter cultures of Bacillus subtilis subsp. innacosarum. Inaquosorum was cultured in a shaking incubator, with the temperature maintained between 30-35 degrees Celsius. And the rotational speed should be between 120-170 revolutions per minute for 5-7 days. Selected and purified strains of B. subtilis subsp. Inaquosorum were then cultured in... Sterilized liquid food, with controlled temperature and incubation speed, is sterilized by steaming. The culture is inoculated at a temperature of 121 degrees Celsius, a pressure of 15 pounds per square inch, for 15 minutes. B. Mixing the microorganism B. subtilis subsp. Inaquosorum with the substrate. Use 32.26-35.28% of the B. subtilis subsp. / naquosorum culture prepared in step A. By weight, mix together with the sterilized support material. Sterilize at a temperature of 121°C. Celsius degrees, 15 pounds per square inch pressure for 15 minutes, containing a quantity of talcum powder. 61.29-64.52% by weight and carboxyl methyl cellulose in the amount of 3.22-3.23%. % by weight. Mix thoroughly and evenly. C. Dehumidification process The microbial product containing B. subtilis subsp. Inaquosorum, mixed with the substrate, is then subjected to a moisture reduction process. Suitable methods include air drying at a temperature between 35-40 degrees Celsius and then grinding it into a fine powder.

2. The process of preparing microorganisms to reduce the release of ammonia that occurs during the organic fertilizer composting process. Under Claim 1, the preparation of the B. subtilis subsp. Inaquosorum starter culture involves providing a liquid medium containing: Modified starch (Soluble Starch) 0.0133-0.0135% by weight. Sucrose 0.033-0.035% by weight. Peptone 0.060-0.065% by weight. Dipotassium phosphate (K2HPO4) 0.0050-0.0055% by weight. Magnesium sulfate heptahydrate (MgSO4.7H2O) 0.0010 -0.0015 % by weight Sodium chloride (NaCl) 0.0010-0.0015% by weight. Ammonium chloride (NH₃,Cl) 0.050-0.055% by weight. Carboxymethyl cellulose 0.0050-0.0055% by weight. And add water until it reaches 100% by weight.

3. The process of preparing microorganisms to reduce the release of ammonia that occurs during the organic fertilizer composting process. Under Claim 1, the preparation of the starter culture of B. subtilis subsp. Inaquosorum provides the required amount of bacteria. The optimal ratio is between 10⁸ cells and 10⁻¹⁰ cells per 1 milliliter of liquid medium.

4. The process of preparing microorganisms to reduce the release of ammonia that occurs during the organic fertilizer composting process. Under Claim 1, an appropriate dehumidification process is provided to reduce the moisture content of the microorganism B. subtilis. Subsp. Inaquosorum was mixed with the substrate until the moisture content did not exceed 10 percent by dry weight.

5. Products obtained from the microbial preparation process for reducing ammonia release that occurs during... The organic composting process under any one of claims 1 through 4, which contains the bacterium B. subtilis subsp. Inaquosorum content must be at least 108 cells per gram.