Probiotic bead formula containing okra extract.

Abstract

ocr The probiotic bead formula containing okra extract includes: okra extract and probiotic bacteria. Probiotics, starch, film-forming agent, sweetener, and distilled water. The suitable probiotic strain is Bifidobacteria. The bacteria Bifidobacterium animalis, found in okra beet seeds containing probiotics, has... The probiotic density ranged from 2.33 x 10⁻¹⁶ colonies / gram (CFU / g), with an average diameter of hundreds of colonies. By reducing the size of the beads before swelling to 123.60%, the resulting beads swell and retain their spherical shape, enclosing the probiotic bacteria. The bacteria are encased within the beads, protecting the probiotics from environmental conditions, thus maintaining their stability and viability. It is present in high quantities for use in functional food products and probiotic-fortified seasonings. Various foods and beverages play a health-promoting role in helping to restore internal balance and promote well-being. Good physical health

Claims

OCR 10KL (16 / 07 / 2569) 1. The probiotic bead formula containing okra extract includes the following ingredients: Okra extract 0.25 - 1 percent by weight / volume. Probiotic bacteria 5-15% by volume / volume. 2-4 percent flour by weight to volume. Film-forming agent 0.5 - 4 percent by weight per volume. Sweeteners 2.5 - 25 percent by weight per volume. Distilled water 51 - 89.75 percent by volume.

2. A probiotic bead formulation containing okra extract, as per claim 1, which contains... The appropriate ingredients are: Okra extract 0.25% by weight / volume. 10% probiotic bacteria by volume 2 percent flour by weight to volume. 2 percent film-forming agent by weight / volume. Sweetener 17 percent by weight / volume. Distilled water 68.75 percent by volume.

3. A probiotic bead formulation containing okra extract, as per claim 1 or 2, where: It is prepared by mixing probiotic cultures with film-forming agents, starch, and okra extract. Then, the entire mixture is prepared... Mixed with sweeteners, the mixture is prepared into beads using the extrusion technique and dropped into a calcium solution. A chloride solution of 0.5% by weight / volume was used, and the residue was washed off with distilled water. The area was then dried. Using the lyophilization technique at temperatures of -10 to -30 degrees Celsius in a vacuum for 24-48 hours. hour 4. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-3. Where probiotic bacteria can be selected from Bacillus coagulans or Bifidobacterium a. Bifidibacterium adolescentis or Bifidobacterium animalis Bifidobacterium animalis, or Bifidobacterium bifidum, or Bifidobacterium breve (Bifidobacterium breve) or Bifidobacterium infantis or Bifido Bifidobacterium lactis or Bifidobacterium longum Or Bifidobacterium pseudolongum or Enterococcus duran. (Enterococcus durans) or Enterococcus faecium or Lactobacillus Lactobacillus acidophilus, also known as Lactobacillus crispatus, Lactobacillus gasseri or Lactobacillus johnsonei Lactobacillus johnsonii), Lactobacillus paracasei, or Lactobacillus reuteri (Lactobacillus reuteri) or Lactobacillus rhamnosus or Lactobacillus Lactobacillus salivarius, Lactobacillus zeae, or Propioni Propionibacterium arabinosum or Staphylococcus scientulis (Staphylococcus sciuri) or Saccharomyces cerevisiae subspecies bualadi (Saccharomyces cerevisiae) Either one of the two or more of the aforementioned probiotic strains (such as subsp. Boulardi), or a combination of both.

5. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-4. The suitable probiotic strain is Bifidobacterium animalis.

6. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-5. Where okra extract is prepared by fermenting crushed okra in a solvent of choice, which can be water or... Ethanol, either alone or in a mixture, in a ratio of okra to solvent of 1:3 to 1:

5. The mixture is steeped by weight to volume for 4-6 hours, then squeezed or pressed to separate the liquid. The liquid is then centrifuged at 5,000 rpm. 8,000 revolutions per minute for 10-15 minutes. Then filter the clear liquid and evaporate it using a distillation apparatus. The substance was evaporated using a rotary evaporator and dried using lyophilization techniques at temperatures of -10 to -30 degrees Celsius. Celsius in a vacuum using a freeze dryer for 12-18 hours.

7. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-6. Where the flour can be selected from rice flour, glutinous rice flour, tapioca flour, corn flour, or bean flour. Green starch, tapioca starch, or wheat flour, either individually or in combination with two or more of the aforementioned flours. The most suitable flour is glutinous rice flour.

8. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-7. Where the film-forming agent can be selected from alginate, sodium alginate, or amylose. (amylose), or carageenan, or cellulose acetate phthalate. Or cellulose acetate trimellitate, or chitosan, or gelatin. Gelatin, chondroitin sulfate, or cyclodextrin. Dextran, also known as D-mannuronic acid or poly(methacrylic acid coethyl) acrylate) 1:1 (poly(methacrylic acid-co-ethyl acrylate) 1:1) or poly(methacrylic acid-co-ethyl) Poly(methacrylic acid-co-ethyl acrylate) 1:1 L 100 or poly(methacrylic) Poly(methacrylic acid-co-ethyl acrylate) 1:1 L 100-55 Or poly(methacrylic acid-co-ethyl acrylate) 1:1 L30D acrylate) 1:1 L 30 D) or poly(ethyl acrylate, comethyl methacrylate, cotrimethyl ammonioethyl methacrylate) chloride) 1:2:0.1 (poly(ethyl acrylate-co-methyl methacrylate-co-trimethylammonioethyl methacrylate chloride) 1:2:0.1) or poly(methacrylic acid comethyl methacrylate 1:2 (poly(methacrylic Acid-co-methyl methacrylate) 1:2), or guar gum, or hydroxypropyl methylcellulose acetic acid. Hydroxypropyl methylcellulose acetate succinate or hydroxypropyl methyl Hydroxypropyl methyl-cellulose phthalate 55 (or hydroxypropyl methyl) Hydroxypropyl methyl-cellulose phthalate 55, also known as inulin, is a chemical compound used in cellulose phthalate. Lactose, also known as L-guluronic acid or locust bean gum, Pectin, also known as polyvinyl alcohol or polyvinyl acetate phthalate. (polyvinyl acetate phthalate), or Shellact, or polysorbate 80, or Whey protein or xanthan gum, or any of the aforementioned film-forming agents. A mixture of two or more substances is used, with suitable film-forming agents being sodium alginate and pectin. (pectin) 9. A probiotic bead formulation containing okra extract, in accordance with any one of claims 1-8. Where the sweetener can be chosen from glucose, sucrose, or galactose. Or dextrose, or sucralose, or maltodextrin, or Aspartame, also known as saccharins, neotam, or advantam. Advantame, stevia, or acesulfame, or any other sweetener. The above are mixtures of two or more types of sweeteners, with sucrose and maltodextrin being suitable sweeteners. Maltodextrin 10. A method for preparing probiotic starter cultures according to any one of claims 1-9, which includes the following steps: a. Cultivate pure stock cultures of probiotic bacteria in a culture medium. Liquid Bifidobacterium broth or liquid MRS culture medium. broth) for probiotic strains of Bifidobacterium, using sterile culture medium for stock. Pure microbial cultures in a volume ratio of 10:1 to 3:1, with the optimal ratio being 5:

1. B. Incubate the mixture from step A using a rotating shaking method at a speed of 80-150 revolutions per minute. At a temperature of 35-37 degrees Celsius for 5-12 hours, with the optimal time being 6-8 hours. C. Next, increase the number of bacteria from the culture obtained in step B by using a Bifido suspension. The prepared Bifidobacterium animalis bacteria were then added to the Bifidobacterium culture medium. Sterile liquid type (Bifidobacterium broth) in a volume ratio of 1:5 to 1:

1. The ideal ratio is 1:2, then incubate using a rotating shaking method at a speed of 150 revolutions per minute at a temperature of 35-37 degrees Celsius. For 6-8 hours. d. Then, centrifuge it at 5,000 - 8,000 revolutions per minute for 10 - 15 minutes to obtain sediment. To obtain the probiotic starter culture, pour off the top liquid.