A method for preparing yeast starter cultures for ethanol production from cassava using the cellulosic ethanol process.

Abstract

OCR 10KL (16 / 09 / 2568) A method for preparing yeast starter cultures for ethanol production from cassava using the cellulose process. Cassava ethanol is produced using a process that utilizes cassava pulp. After the distillation and centrifugation process using a separator to separate the solids and water, the moisture content is adjusted, and then... Heat is applied in a pressure reactor, separating the solid and liquid while maintaining moisture in the solid. Add... The cellulase enzyme is then used to mix the slurry, separating the solids and liquid. The yeast culture is then added to the liquid. The powdered or fresh yeast is stirred and mixed, then all the liquid is added to the fermentation reactor.

Claims

OCR 10KL (16 / 09 / 2568) 1. The process for preparing yeast starter cultures for ethanol production from cassava using the cellulosic ethanol process involves the following steps: A. Cassava sludge is processed by taking cassava sludge that has been distilled and separated using a pulp and water separator, adjusting the moisture content to be no less than 85%, and then heating it in a pressure reactor at a temperature of 160-200 degrees Celsius, while maintaining... Pressure at that temperature range for 15 minutes. B. Separate the solids and liquids while maintaining the moisture content of the solids at 65-75%. C. Add cellulase enzyme and stir the slurry for 24 hours at a temperature of 45-55 degrees Celsius to break down the cellulose chains into sugars. d. Separate the solids and liquids. Add powdered or fresh yeast to the liquid, maintaining a cell concentration of 1 x 10⁷ cells per milliliter. Stir the mixture under these conditions for 24 hours. Finally, add the remaining liquid to the fermentation reactor.

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