A method for preparing propolis extracts containing alpha-mangostin and gamma-mangostin.

Abstract

ocr A method for preparing propolis extracts containing alpha-mangostin. And gamma-mangostin starts with cutting the propolis from stingless bees into small pieces, then... Extraction is performed using ultrasonic waves with ethanol mixed with water as a solvent, then the substance is evaporated to a viscous consistency. The extract was then concentrated and dissolved in ethanol, then mixed again with water, and the dissolved portion was used. Wash with hexane, then mix the resulting ethanol extract with water and extract it using the shaking method. Dichloromethane was then centrifuged to separate the layers, and the extract from the layer was then taken. When dichloromethane is evaporated, it yields a yellowish-brown extract from the propolis of stingless bees. sugar

Claims

ocr 1. A method for preparing an extract from stingless bee propolis containing alpha-mangostin. And gamma-mangostin, which has the following steps: A. Cut the propolis from stingless bees into small pieces and mix it with the solvent in the ratio of 10 grams of propolis to 10 grams. Add 400 milliliters of solvent, using ethanol, diluted with distilled water to achieve the desired consistency. Concentrated at 50-60% volume / volume, extracted using ultrasonic waves at 40 kHz at a temperature of 30-40°C. Incubate at Celsius degrees for 30 minutes. After the time is up, filter the mixture using cotton wool. Collect the filtered extract, then add the... Dilute 400 milliliters of solution, using ethanol as the solvent, with distilled water to a concentration of 100%. Dissolve 50-60% of the volume into the resin again, then extract using high-frequency sound waves of 40 kHz. At a temperature of 30-40 degrees Celsius for 30 minutes, filter the mixture using cotton wool. Collect the filtered extract. The extracts obtained from both filtration processes are then combined and condensed using an evaporator. Rotating at 45-50 degrees Celsius will yield a semi-solid, dark brown extract called the extract. rough B. Wash and extract the crude extract obtained from step A again using a separating funnel. The crude extract was dissolved in 55% ethanol at a ratio of 1:

20. Alternatively, use 1 gram of crude extract per 20 milliliters of solvent, filtered through filter paper with a pore size of [specify pore size]. 11 microns. Then, take only the soluble portion and add hexane solvent in a 1:5 ratio. By volume, shake the separating funnel for 5 minutes. Let it stand to separate the substances and discard the hexane. Repeat this process 4-5 more times until the hexane layer becomes clear. C. Mix the ethanol extract obtained from step B with dichloromethane. (dichloromethane) by adding dichloromethane in a ratio of 1:5 by volume. Shake with a shaker at a speed of 100-120 revolutions per minute for 15 minutes, then centrifuge to separate. The layers were separated at a speed of 10,000 revolutions per minute for 3 minutes, and the ethanol was collected for extraction. The process was repeated with dichloromethane, combining the extract from the dichloromethane layer. The solvent from the dichloromethane is completely evaporated using a rotary evaporator.