A method for producing crude extract from rice fermentation using Galactomyces yeast starter culture.

Abstract

OCR 09WP 23 / 06 / 2569 The process for producing crude extract from rice fermentation using Galactomyces yeast starter culture involves the following steps: The invention involves three unique steps: 1. Preparing the dough from the inoculum of the fungus Amyomyces rosii. (Amylomyces rouxii TISTR 3182) for use in rice fermentation. 2. Rice fermentation to prepare food for use in... 1. Cultivation of Galactomyces yeast and 3. Fermentation of Galactomyces yeast for the production of substances. Crude extract was obtained using a fungal inoculum containing Amylomyces rouxii (TISTR 3182). Its composition and distinctive features include the use of buffer solutions, surfactants, and glass beads in its production process. The fungus spreads throughout the fermentation process of various types of rice, resulting in a sweetened liquid with a sugar content of 30-45. The Brix value that can be used as a culture medium for the yeast genus Galactomyces is Galactomyces candidus. (Galactomyces candidus TISTR 3686) in the production of crude extract from the yeast genus Galactomyces.

Claims

OCR 09WP 23 / 06 / 2569 1. A method for producing crude extract from rice fermentation using Galactomyces yeast starter culture, which has... The steps are as follows: 1.1 Preparation of dough balls from the inoculum of the fungus Amylomyces rouxii (TISTR 3182) for... Used in fermenting rice. A. Weigh the flour, which can be either glutinous rice flour or regular rice flour, in an amount of 100-500 grams. Grams are packed into heat-sealable bags, 10-50 grams per bag, and then sterilized by steaming at 121 degrees Celsius. Press to a pressure of 15 pounds for 15 minutes. Then bake to dry at 40-80 degrees Celsius for 12-24 hours. Before use, sterilize by steaming and drying again at the same temperature and time. B. Transfer the culture of Amylomyces rouxii (TISTR 3182) from the agar plate. Culture the selected microorganisms using potato dextrose agar plates. Sabouraud Dextrose Agar (SDA), also known as Dichloram Rose Bengal. One of the following types of chloramphenicol agar (Dichloran Rose-Bengal Chloramphenicol Agar; DRBC): Incubate at 25-30°C for 7-14 days in 6-8 dam tubes containing protozoa culture medium. Potato dextrose agar (PDA), Sabouraud dextrose agar Dextrose Agar (SDA) or Dichloran Rose-Bengal Chloramphenicol Agar. Use 3-10 ml of one type of Chloramphenicol Agar (DRBC) in 30 vials and incubate further. At a temperature of 25-30 degrees Celsius for 3-5 days, after the specified time, scrape off the mycelium of the fungus Amylomyces rosii. (Amylomyces rouxii TISTR 3182) 15-30 tubes from the esophagus are placed in a buffer solution which can... Choose between Buffered Peptone Water (BPW) or Phosphate Buffered Saline. One type of phosphate buffer saline (PBS) at a concentration of 0.1-1 percent is used to measure absorption values. Light in the 400-600 nanometer range has an absorption value in the range of 0.5-2. Then, a surfactant is added, which... You can choose from Triton X-100, Digitonin, or Polysorbet 20. 20) Polysorbet 80, Sodium Dodecyl Sulfate (SDS), Deoxy Deoxycholate, Cholate, Sarkosyl, Cocamidopropyl Betaine (Cocamidopropyl Betaine; Coco Betaine) Amphoteric Surfactant A Ethoxylated alcohol, alkyl glucoside, or alkyl polyglucoside. Add one of the following alkyl polyglucosides at a concentration of 0.05-0.1 percent: Add 50-100 grams of sterilized glass beads, 1-5 millimeters in size, at a temperature of 121 degrees Celsius. At Celsius, pressurize at 15 pounds for 15 minutes, shaking with a shaker until the fibers are uniformly distributed. C. Mix the glutinous rice flour or regular rice flour prepared in step A with the fungal solution prepared in step B. Using a ratio of 10-50 grams of flour to 4-20 milliliters of fungal solution, mix thoroughly in a bag and transfer. Place the mixture in a sterile container, cover with cheesecloth, and leave at 30 degrees Celsius for 5-14 days. After the specified time, the prepared fungal inoculum was dried in an oven at 40 degrees Celsius for 2-3 days. 1.2 Fermenting rice to prepare a culture medium for culturing Galactomyces yeast. Rice, which can be selected from Thai rice varieties with their own unique aroma and characteristics, includes Jasmine rice and various glutinous rice varieties. Any one of the following: Khao Wong Kalasin glutinous rice, Khao Khiao Ngou glutinous rice, black glutinous rice, brown rice, or Riceberry rice. Or more than one type mixed together, in quantities of 1-20 kilograms. Then soak the rice in 2-40 liters of distilled water for a certain period of time. Let the rice sit for 6-24 hours before steaming for 30-45 minutes until cooked. Once cooked, place the rice on a stainless steel tray to cool. Let the rice cool for an hour, then divide it into portions, each containing 1-5 kilograms, before separating them into different containers. After sterilization, add the prepared Amylomyces rouxii (TISTR 3182) inoculum. Option C. Quantity: 10-500 grams (ratio of fungal inoculum to rice: 1-10 percent) and distilled water. Add 400-2,000 ml of starter culture and mix thoroughly. Incubate at 25-30 degrees Celsius for 1-3 days. Day by day, until sap forms. Measure the sweetness of the sap daily until it reaches 30-45 degrees Celsius. Rix collects the nectar in sterilized containers, filters it through sterile cheesecloth, and then uses it. Settlement is achieved by letting it stand at a temperature of 2-8 degrees Celsius for 24-48 hours. Only the clear portion is used. The fermentation of the yeast genus Galactomyces continues. 1.3 Fermentation of yeast of the genus Galactomyces to produce bioactive compounds. Inoculate the cultured Galactomyces spp. on a selectable agar plate. From Potato dextrose agar (PDA), dichloram rosebengal, chloramphenicol A. Dichloran Rose-Bengal Chloramphenicol Agar (DRBC), Maltose Yeast Extract Agar (Maltose Yeast Extract Agar; MYA) or Yeast Malt Agar (YM Agar), which type? One method involves incubating at 25-30 degrees Celsius for 3-7 days, during which a yeast strain of the genus Galactus can be selected. The organism Galactomyces candidus (TISTR 3686) was cut into pieces of a certain size. Place 3-5 pieces of 1x1 cm material into a nectar solution, which is then diluted with sterile distilled water to a concentration of 5-45%. The Brix degree obtained in step 2 is then incubated at 25-30 degrees Celsius for 4-7 days. Centrifugation at 2,000-8,000 rpm for 10-20 minutes, or membrane filtration. The size ranges from 11-0.22 microns. Yeast cells are extracted and placed in sterilized containers for storage. The temperature should be maintained at 2-8 degrees Celsius before the crude extract obtained from the fermentation of Galactomyces yeast is used.