A two-stage process for optimizing biogas production from cassava waste.

Abstract

OCR 09WP 27 / 07 / 2569 This invention concerns the enhancement of biogas production by demonstrating the use of microorganisms. A group of Bacillus bacteria capable of producing enzymes that break down the components of cassava pulp. Biocatalysts in the biogas production process from cassava waste, in the form of a gasification process. A two-stage biological process consisting of a raw material pretreatment step and a raw material degradation step. It does not use oxygen, which results in an increase in the amount of biogas and methane produced, as well as an increase in the rate of biogas production. This results in improved efficiency in the biogas production process and the management of cassava waste. Waste materials from cassava processing.

Claims

OCR 09WP 27 / 07 / 2569 1. The two-stage process for optimizing biogas production from cassava pulp consists of: a) The raw material conditioning step is done by conditioning the cassava pulp under aerobic conditions. By using Bacillus spp. bacteria, which are capable of producing the enzymes cellulase and xylanase. Pectinase and amylase, either individually or in combination, at temperatures of 25-35 degrees Celsius. The initial pH range is between 5-8 and lasts for 8-72 hours. b) The methane production process is carried out by fermenting the cassava pulp obtained from step a) to produce methane. Anaerobic conditions, using an anaerobic digestion process at temperatures of 25-45 degrees Celsius. The initial Celsius and pH levels are in the range of 6.7-7.

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2. A two-stage process for optimizing biogas production from cassava waste, as per the claim.

1. Where Bacillus spp. bacteria can be selected from Bacillus subtilis, Bacillus pumilus, Bacillus Tequilensis, either individually or in combination.

3. A two-stage process for optimizing biogas production from cassava waste, as per the claims.

1. Where Bacillus spp. bacteria can be selected from Bacillus subtilis subsp. subtilis TBRC 7031, Bacillus pumilus TBRC 2887, Bacillus tequilensis TBRC 6949, B. tequilensis TBRC 9019 Either one or a combination of several.

4. A two-stage process for optimizing biogas production from cassava waste, as per the claim.

1. Where the biogas production process can be carried out in a selectable batch fermentation method. Or a semi-batch system that involves adding cassava pulp (fed-batch), or either one.

5. A two-stage process for optimizing biogas production from cassava waste, as per the claim.

1. The ratio of cassava pulp to Bacillus spp. bacteria during the pulp conditioning step. Cassava pulp is in the range of 5-50 grams of total solid cassava pulp per 100 milliliters. Bacillus spp. bacteria cultured in LB liquid medium.

6. A two-stage process for optimizing biogas production from cassava waste, as per the claim.

1. This process can be achieved by adding enzymes from the bacteria Bacillus spp. The process of conditioning cassava pulp, instead of adding Bacillus spp. bacteria.

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