A biomass digestion process for non-gelatinized starch and lignocellulose materials using a mixed enzyme formula.

Abstract

OCR 10KL (24 / 10 / 2568) Biomass digestion methods that use unprocessed starch and lignocellulose materials. Gelatinization is achieved using a mixed enzyme formula by mixing the enzyme blend and incubating the biomass containing starch and lignostic materials. Cellulose is a component that exists under conditions of temperature of 40-60 degrees Celsius and a pH of 4.0-6.0. Rotation speed of 200 revolutions per minute for 0.5-75 hours.

Claims

OCR 09WP 06 / 05 / 2569 1. Biomass digestion methods that use starch and lignocellulose materials that are not gelatinized. With a blend of enzymes, it contains: - Starch-digesting enzymes work together with lignocellulose-digesting enzymes to produce sugar molecules. The individual steps are as follows: A. Mix the enzyme formula in a concentration of 0.5-10.0 milligrams per gram of substrate. B. Cultivate biomass containing starch and lignocellulose materials under conditions of a temperature of 40°C. 60 degrees Celsius, pH value 4.0-6.0, rotational speed 200 revolutions per minute, for 0.5-75 hours. Where starch-digesting enzymes can be selectively chosen from glycosyl hydrolases. (Glycoside hydrolase), which includes enzymes in the amylase and glucoamylase groups, with the following quantities: The recommended dosage is 1-5 milligrams per gram of substrate. Where lignocellulose-degrading enzymes can be selectively chosen from glycosyl hydrolases. (Glycoside hydrolase) which includes enzymes in the cellulase and hemicellulase groups.

2. Biomass digestion methods using starch and lignocellulose materials that are not gelatinized. With the enzyme mixture formula, as per claim 1, where the starch content in the starch-containing biomass and lignostic material. Cellulose makes up 30-70% of the composition by weight.