The process of producing biogas from Napier grass mixed with organic waste using a selective anaerobic microbial process.

Claims

OCR09WP14 / 08 / 25691. Biogas production process from Napier grass mixed with organic waste using a mixed anaerobic microbial process consisting of the following steps: a. Preparation of the main biomass raw material, Napier grass, which is reduced in size using mechanical methods. b. Preparation of the co-biomass raw material, wet sludge. c. Mixing the main biomass raw material with the co-biomass material in a weight ratio of 2-5 parts main biomass to 1 part co-biomass, resulting in an organic loading rate of 1- 1. 1.5 kg of volatile solids per cubic meter per day (kgTVS / cubic meter reactor-day). d. Prepare an anaerobic microbial inoculum mixture derived from a combination of at least two or more types of animal manure. e. Mix the biomass raw materials from step c with the microbial inoculum from step d and perform continuous fermentation in a continuous reactor (CSTR), maintaining the fermentation temperature between 30-45 degrees Celsius. f. Ferment the mixture from step e for a retention time of 40-80 days in a multi-stage reactor.The process of biogas production from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to claim 1, where the suitable Napier grass is classified as Pak Chong 13. The process of biogas production from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to claim 1 or 2, where the suitable Napier grass has a harvest time for biomass use of 60 to 90 days from planting.

4. The process of biogas production from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to any one of claims 1 to 3, where the wet sludge is classified as wet sludge obtained from ethanol production from cassava.

5. The process of biogas production from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to any one of claims 1 to 4, where the type of animal manure that can be used is selected from at least two or more types of pig, cow, buffalo, or chicken manure combined. 6.The biogas production process from Napier grass mixed with organic waste using a mixed anaerobic microbial process according to claim 5, where the appropriate type of animal manure to be mixed with cow manure and pig manure is specified.

7. The biogas production process from Napier grass mixed with organic waste using a mixed anaerobic microbial process according to claim 6, where the appropriate proportions of pig and cow manure used to make the mixed anaerobic microbial inoculum consist of: - Cow manure 10-30% by weight - Pig manure 70-90% by weight.

8. The process of producing biogas from Napier grass mixed with organic waste using a mixed anaerobic microbial process according to patent 7, where the mixed anaerobic microbial culture derived from cow and pig manure contains the following proportions: - Clostridia 5-35% of the total microbial population - Bacteroidia 2-12% of the total microbial population - Bacilli 2-10% of the total microbial population - Actinobacteria 0%.4-5% of the total microbial population consists of metagenic bacteria (metanogens), 1-15% of the total microbial population where at least one metagenic bacterium is selectively bred from metanobacteria, metanosarcinia, and metanomobacteria; and 35-70% of the total microbial population where at least one other microbial bacterium is selectively bred from thermotogae, fibrobacteria, and synergistsia. )Syntrophomonadia, Limnochordia, Thermacetogenia, Caldatribacteriota, Dethiobacteria, Desulfotomaculia, Rhodothermia, Gammaproteobacteria, Syntrophia, Syntrophorhabdia, Cloacimonadia, Anaerolineae, Caldatribacteriia, Hydrogenedentia, and Verrucomicrobiae.9.

10. The process of producing biogas from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to any one of the claims 1-8, where the optimal fermentation temperature is 35-40 degrees Celsius; 11. The process of producing biogas from Napier grass mixed with organic waste using a selective anaerobic mixed microbial process according to any one of the claims 1-9, where the optimal retention time in the aggregate digester is 60 days.