Temperature control unit that minimizes electrical noise in in vitro and in vivo experiments.

Abstract

The invention relates to a temperature control unit that enables precise heating of both tissue and the fluid supplying it to the desired temperature during microscopic examination of living cells and tissues, and whose electrical noise is either invisible or minimal in electrophysiological recordings, and which allows for the maintenance of stable body temperatures for extended periods in animals under anesthesia.

Description

1 TARIFF Minimizing electrical noise in in vitro and in vivo experiments. TEMPERATURE CONTROL UNIT TECHNICAL AREA 5 The invention enables the examination of both tissue and living cells and tissues under a microscope. and precisely heating the fluid that nourishes the tissue to the desired temperature, and electrical noise that is not visible at all in electrophysiological recordings or that is not visible in recordings Very little visible, not enough to have an effect, and prolonged exposure to animals under anesthesia It involves a temperature control unit that ensures body temperatures are kept constant. 10 PREVIOUS TECHNIQUE Live cell and live tissue research (In-vitro studies) In in-vitro electrophysiological studies performed by viewing under a microscope, 15 special microscope-compatible substrates on which cells or tissues are placed. A chamber is needed. Chambers generally have a heating system. They are not, and heating the cell and tissue involves heating the solution that nourishes the cell. This is provided. Recently, electrically heated chambers have been introduced to the market. (https: / / science-products.com / en / shop / 321 / 45 / perfusion-recording-20 tissuehandling / temperature-control / thermal-stages / wp-10). Electrically heating the chamber allows the tissue or cells to be extracted. It can generate electrical noise in electrophysiological recordings because in-vitro Electrophysiological recordings are very sensitive and can detect voltages at the microvolt level. This requires recording. The electrical heating of the Chamber also brings with it 25 It causes major problems, or even becomes inoperable, when working at sensitive voltages. This leads to the following: In our invention, the chamber we designed is made from an aluminum block. It was constructed and aluminum tubes were passed through the bottom of the chamber. Aluminum The chamber is heated by passing water at the desired temperature through the pipes. Contact with the chamber... Since there is no electrical device involved, no electrical noise is generated during recording. 30 Additionally, the chamber has a sensor that continuously measures the temperature, and the temperature... If the temperature drops, the water passing through the aluminum pipe is heated to the desired level. It is automatically adjusted with an accuracy of ±0.1 °C. 2 Testing different drugs and molecules on living cells or tissues. recording the electrophysiological changes in the cell or tissue as a result In their studies, the solution(s) are frequently heated before being introduced into the chamber and the tissue is examined. This ensures that the temperature is adjusted accordingly. The process of heating the solutions involves a fine-tuning process. by operating an electric heater placed inside the tube and the solution inside this tube 5 This is achieved by passing it through. The product that works with this system is shown in the link. (https: / / science-products.com / en / shop / 329 / 46 / perfusion-recording- tissuehandling / temperature-control / inline-heaters / sh-27b). However, the voltage and current used to heat the solution were also recorded in the electrophysiological data. This can lead to the generation of electrical noise, which in turn can corrupt the recordings. 10 In addition, multiple heaters are needed when multiple molecules or drugs need to be administered. This is noticeable. Situations requiring multiple heating elements also lead to a reduction in the microscope field. and restricts or prevents work in this area with other tools. Our Thanks to the chamber design in our invention, we can operate separately or at liquid flow rates of 3 ml / min. Even when administering multiple drugs or molecules simultaneously, the temperature inside the chamber remains at 15°C. can be maintained at the desired level. Thus, a heating system is not required for each solution. It is not audible. Live animal research (In-vivo studies) In live animal research, the body temperature of animals under anesthesia is kept at 20°C. It is desirable to prevent loss, and for this purpose heating lamps, heating plates and electric Blankets have been manufactured. Related products and their websites are: 1) https: / / science-products.com / en / shop / 294 / 128 / in-vivo / temperature-measuring- systems-1 / animal-temperature-control / hl-1 2) https: / / science-products.com / en / shop / 297 / 128 / in-vivo / temperature-measuring-25 systems-1 / animal-temperature-control / hp-1m 3) https: / / science-products.com / en / shop / 295 / 128 / in-vivo / temperature-measuring- systems-1 / animal-temperature-control / wb-1 While these heating systems are sufficient for stages such as surgical procedures, they are used on animals. Electrophysiological recording may result in electrical noise. 30 Our invention includes two designs. Our invention involves the end of the water heating system. The chamber in the part is removed and replaced with (1) a blanket through which a water pipe passes or (2) Heating is achieved through copper pipes through which water passes under a metal plate. (Heating system) Using hot water also eliminates electrical noise. 3 This is provided by a temperature measurement device placed on the animal's body. This is done automatically with the sensor. THE PURPOSE OF THE INVENTION Information obtained from living cells, living tissues, and experimental animals is used in health. 5 These are important experimental researches in the field of science. Our discovery falls into two categories. It is available for use. 1. Live cell and live tissue research (In-vitro studies) Microscopic examination of living cells and tissues reveals that life is present for 10 years. To preserve the tissue for a long time, it is necessary to maintain a constant temperature. For this purpose... The solution necessary for tissue nourishment must also be at the desired temperature. This requires the design of electrical appliances and devices to be used in these heating processes. and temperature adjustment accuracy and electrical performance depending on the material used in its production. The noise level varies. Our invention allows us to examine living cells and tissues under a microscope for 15 minutes. During examination, both the tissue and the fluid nourishing the tissue must be precisely at the desired temperature. Heating and electrical noise were not recorded in electrophysiological recordings. invisible or barely visible temperature control that will not affect the records It will be used in the processes. 2. Live animal research (In-vivo studies) In the laboratory, small live animals (mice, rats, guinea pigs, etc.) are subjected to anesthesia. In research conducted under these conditions, it is necessary to keep the animal's temperature constant. Our discovery allows these animals to maintain a constant body temperature for extended periods while under anesthesia. It will be used in the capture of live animals. With our invention, 25 electrophysiological recordings show no or very low electrical noise in the working environment. It will provide. In-Vitro: In our invention, the reservoir we designed was made from an aluminum block. Aluminum pipes are passed through the bottom of the reservoir. The desired shape is obtained from these aluminum pipes. The reservoir is heated by passing water at a certain temperature through it. An electrical appliance that comes into contact with the reservoir is 30°C. Because there is no electrical noise during recording, the reservoir also does not produce electrical noise during recording. It has a sensor that constantly measures the temperature, and when the temperature drops... In this case, the water passing through the aluminum pipe is heated to the desired temperature of ±0.1 °C. It is automatically adjusted with precision. 4 Thanks to the reservoir design in our invention, separate liquid flows of 3 ml / min are possible. Even if multiple drugs or molecules are administered separately or simultaneously, within the reservoir The temperature can be maintained at the desired level. Thus, a heating system is required for each solution. It is not needed. This both reduces costs and is necessary in the current situation. Space is saved because the heating systems used are moved away from the microscope. It can provide heating without producing electrical noise. In-Vivo: At this stage, our invention includes two designs. In our invention, the reservoir at the end of the water heating system is removed and replaced with a system that pumps water from inside. heating with copper pipes through which water passes under a blanket or metal plate that runs over the pipe. This is being done. Using hot water as a heater also reduces electrical noise by 10%. This ensures its elimination. The temperature control is done by placing a device on the animal's body. This is done automatically using a temperature measuring sensor. LIST OF FIGURES Figure 1. Assembled isometric view of the invention. 15 Figure 2. Assembled front view of the invention. Figure 3. Assembled isometric view of the invention. Figure 4. Assembled top view of the invention. Figure 5. Exploded view of the invention. Figure 6. Exploded top view of the invention. 20 Figure 7. Exploded bottom view of the invention. Figure 8. Bottom view of the blanket / metal plate system. Figure 9. Top view of the blanket / metal plate system. The corresponding numbers in the figures are: 25 1. Electronic box cover 2. Water heating tank lid 3. Water heating chamber 4. Water heating chamber base 5. Water circulation pipes 30 6. Digital display screen 7. Reservoir body 8. Heating element cable and water tank temperature sensor cable. 9. Reservoir sensor cable 10. Reservoir temperature sensor hole 11. Water circulation pump 12. Temperature adjustment lever 13. Power switch 14. Heater resistance connector and water tank temperature sensor connector 5 15. Reservoir sensor connector 16. Electronic control unit enclosure base 17. Control unit ventilation holes 18. Power cord clip 19. Water heater tank lid handle 10 20. Sample container, glass base. 21. Reservoir heating aluminum tube 22. Reservoir temperature sensor 23. Toroidal transformer 24. EMI / RFI line filter 15 25. Rectifier, regulator, filters, data acquisition and main control circuit board. 26. Pump and resistance drive 27. Sensor and resistor cable housing 28. Sensor and resistor cable entry hole 29. Water inlet and outlet nipples 20 30. Heating resistors 31. Water tank heating elements 32. Water tank temperature sensor 33. Blanket / metal plate system 34. Hot water outlet 25 35. Hot water inlet 36. Pipeline DETAILED DESCRIPTION OF THE INVENTION The invention minimizes electrical noise in in vitro and in vivo experiments. temperature control unit; electronic box, water tank, reservoir, water circulation pump (11), It includes water circulation pipes (5) and blanket / metal plate system (33) units. Electronic control unit; electronic box cover (1), digital display screen (6), temperature adjustment lever (12), power switch (13), heater resistor connector and water tank 6 temperature sensor connector (14), reservoir sensor connector (15), electronic control unit box base (16), control unit ventilation holes (17), power cable clip 18), toroidal transformer (23), EMI / RFI line filter (24), rectifier, regulator, filters, data acquisition and main control circuit board (25), pump and resistance driver (26) parts It includes. Temperature adjustment lever (12), for adjusting the temperature on the digital display. It is used. The power switch (13) supplies electric current to electronic systems. It is used for switching on and off during operation. Heating element connector and water. reservoir temperature sensor connector (14), where the temperature sensor is placed, resistor and is where the connection cables for the sensor enter. Reservoir sensor connector (15), This is where the cable for the temperature sensor in the chamber enters. Control unit ventilation 10 holes (17) allow the temperature that may be generated inside the control unit to reach the room environment while it is operating. It provides conventional transfer. Power cable clip (18), power It prevents the cable from breaking due to stresses caused by pulling on it. Toroid The transformer (23) provides the conversion of the energy required for the device. EMI / RFI Line filter (24) ensures that sensitive electronic components are correctly 15 without being affected by external signals. It ensures that it works in a certain way. Rectifier, regulator, filters, data acquisition and main control circuit board (25), electronic components necessary for the operation of the system It is the circuit board in which the pump and resistor driver (26) is located. It adjusts the electrical input required for its operation. Water tank; water heating tank lid (2), water heating tank (3), water heating 20 tank base (4), water heater tank lid handle (19), sensor location and the sensor and resistance cable housing, which is the room where the resistance connection cables are located (27), water hoses should be tightly connected so that water does not leak from the connection point The sensor and resistance cable entry hole (28) that allows water to enter and exit the tank. inlet-outlet nipples (29), heating resistors (30) that provide water heating, resistor 25 water reservoir heating resistors (31) showing the points where the ends enter and exit and water The reservoir contains the temperature sensor (32) parts. Reservoir; reservoir body (7), reservoir temperature sensor hole (10), reservoir sample chamber glass base (20), chamber heating aluminum tube (21) and chamber temperature It includes sensor (22) parts. 30 For in-vitro experiments: Electronic control unit, water tank and water circulation system. pump (11) connected to water circulation pipes (5) and reservoir temperature sensors (22) It is connected to the temperature adjustment lever (12) and the digital display screen on the electronic control unit. 7 (6) The water temperature is set to the desired degree by the user. It can be adjusted. After this adjustment, electricity is sent to the water tank and The water inside the water tank is heated by means of the water tank heating resistors (31). It starts. The water circulation pump (11) is powered via the electronic box. and when the water circulation pump (11) is running, 5 inside the water heating tank (3) water first goes to the water circulation pump (11) then to the reservoir body (7) and then water again It circulates by going to the heating chamber (3). Inside the chamber body (7) Water passing through the aluminum pipes (21) of the reservoir heating transfers its temperature to the reservoir body (7) It causes the reservoir to heat up by transferring heat. The reservoir on the reservoir body (7) When the temperature sensor (22) indicates the desired temperature, the electronic box water heating 10 It stops supplying energy to the reservoir (3). However, water circulation continues continuously. When the temperature of the reservoir body (7) falls below the desired temperature, the water Energy is supplied to the heating chamber (3) and the water is heated. The water heating process is 0.1 oC This is carried out with meticulous control. The digital display on the electronic box... Both the temperature of the water in the water heating tank (3) and 15 can be seen via the display screen (6). The tank body (7) temperature can be displayed separately. For in-vivo experiments: Electronic control unit, water tank, water circulation system. pump (11) and blanket / metal plate system (33) are connected to each other by water circulation pipes. (5) and connected with reservoir temperature measurement sensors (22). The electronic control unit is digital 20 When the water temperature is set to the desired degree on the display screen (6), water heating Electricity is sent to the reservoir (3) and the water inside the water heating reservoir (3) starts to heat up. The water circulation pump (11) receives energy via the electronic control unit. and when the water circulation pump (11) is running, the water inside the water heater tank (3) first turns into water to the circulation pump (11) from there to the blanket / metal plate system (33) and then back again 25 It circulates by going to the water heater tank (3). Blanket / metal plate The system (33) passes through a plastic pipeline (36). Water passes through this plastic pipe. It heats the blanket / metal plate system (33) by passing through the line (36). Plastic pipe The reason for using the line (36) is that the blanket / metal plate system (33) is flexible. and to make it foldable. In the blanket / metal plate system (33) flat 30 Metal pipes (aluminum or copper, depending on preference) under the metal plate and water passes through these metal pipes and blanket / metal plate system (33) It provides heat. The metal plate is made of stainless steel. 8 from plastic / metal pipeline (36) inside blanket / metal plate system (33) The water passing through transfers its heat to the blanket / metal plate system (33) It ensures the heating of the plate system (33). In the in-vivo system, the reservoir temperature sensor (22) It is a portable sensor designed to be placed on an experimental animal. Experimental animal When the temperature sensor (22) on it shows the desired temperature, the electronic control 5 The unit stops supplying power to the water tank (3) but the water circulation continues continuously. It does. When the temperature of the experimental animal drops below the desired temperature, it is added to the water reservoir. (3) energy is supplied and water is heated. The water heating process is controlled with an accuracy of 0.1 oC. This is done via the digital display screen (6) on the electronic control unit. Both the temperature of the water in the water reservoir (3) and the temperature of the experimental animal were separately 10 It can be viewed. 20 30

Claims

9 REQUESTS 1. Temperature control unit that minimizes electrical noise in in vitro experiments. Its feature is that it is connected to each other by water circulation pipes (5) and reservoir temperature The electronic control unit, connected with its sensors (22), water heating tank lid 5 (2), water heating tank (3), water heating tank base (4) and water circulation pump (11) allows the user to adjust the water temperature to the desired degree and Digital display screen (6) that allows the display of temperatures, water reservoir heating resistors (31), water reservoir temperature sensor (32), for example reservoir body (7) where it will be placed, reservoir heating aluminum pipes (21), 10 that the desired temperature has been reached and that the temperature has dropped below the desired temperature It is characterized by containing a reservoir temperature sensor (22) to detect it.

2. Temperature control unit that minimizes electrical noise in in vivo experiments. and features include: electronic control unit, water heating chamber lid (2), water heating tank (3), water heating tank base (4), water tank heating resistors (31), water 15 tank temperature sensor (32), water circulation pump (11) and pipeline (36) including blanket / metal plate system (33), water circulation pipes (5), user the water temperature can be adjusted to the desired degree and the temperatures can be controlled. digital display screen (6) that allows viewing and reservoir temperature It is characterized by containing measurement sensors (22). 20 30

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