An antibacterial suture with improved mechanical properties, made from organic fibers.

Abstract

The invention utilizes hemp fibers to develop superior strength, durability, absorbency, and antibacterial properties. and it relates to an environmentally beneficial surgical suture thread and its production process. These sutures are organic, designed to make the thread more visible during surgery. It was colored with walnut shell dye and sterilized for surgical use. This The aim of innovative sutures is to accelerate the wound healing process and reduce the risk of infection. The goal is to minimize.

Description

1 TARIFF MECHANICALLY PROPERTIES IMPROVED AND MADE FROM ORGANIC FIBERS. AN ANTIBACTERIAL MILK THAT HAS BEEN PROCESSED 5 Technical Field to Which the Invention Relates The invention involves a high-performance, wound-healing compound, specifically containing hemp fibers. strength, durability, absorbency, antibacterial properties, and environmental advantages. It relates to an enhanced surgical suture and its manufacturing method. These sutures... To make the thread easily visible during the operation, 10 organic walnut shell dyes were used. They are stained and sterilized for surgical use. The sutures that are the subject of the invention. Its purpose is to support the wound healing process and reduce the risk of infection. State of the Art The healing time for body tissues that are cut as a result of surgical operations or injuries is 15. bringing them closer together until a solution is found, fusing the prostheses to the tissues, stopping bleeding to tie off blood vessels or channels in order to prevent leakage or other leaks The natural or synthetic, sterile surgical suture material used is called surgical thread or surgical suture. It is called a suture. Surgical threads are used in surgical procedures to hold wounds together and accelerate the healing process. They are essential medical supplies used for support. Surgical sutures are usually two-piece. They are divided into main categories: natural and synthetic. Natural yarns include silk and catgut. While synthetic yarns have a wider range and contain polyglactin, It is made from materials such as polypropylene, nylon, and stainless steel. Absorption They can also be classified according to their capabilities; some fibers are absorbed by the body, while 25 Some are not absorbed and need to be removed after the wound heals. Doctors recommend surgery. They select the most suitable thread according to the type of intervention and the patient's needs. These threads, It supports the healing process by holding wounds together and prevents infection. reduces the risk. Among the most preferred materials for sutures in current techniques are nylon, 30 Materials such as polyester (ethilon, dermalon), silk, and stainless steel are available. 2 Despite their durability, the main disadvantage of nylon is that it doesn't provide good knot security. The difficulty lies in the fact that monofilaments have more memory than braided stitches. (because they tend to revert to their packaged form), correctly They tend to unravel if they are not connected. To obtain a secure end node, the most important thing is... At least four to five carefully formed "shots" or knots are required [1]. Polyester sutures, 5 They are more expensive than most of their alternatives and significantly more expensive than other cheaper sutures. They do not offer an advantage and are therefore not widely used. Additionally, they are braided. In polyesters, bacteria become trapped between the fibers and isolated from phagocytic cells, thus preventing sinusitis. this can lead to the emptying of the airways in the presence of contamination and infection. cannot be used [2]. Silk has some disadvantages: it is not absorbable, its extraction is difficult 10 It is necessary; its multifilament structure leads to bacterial and fluid accumulation, premature withdrawal. This can leave less tension during the process and may cause a greater inflammatory reaction, This increases the risk of the incision line opening [3]. Stainless steel can cut through tissues. It is not useful in very mobile areas, its usability features are poor [2]. The limitations and inadequacies of current technological solutions range from knotting difficulty to 15 risk of leakage, high cost, contamination and infection the risks involved, subsequent removal by a professional Due to reasons such as necessity and the potential for tissue damage, there is a need for a suture in the field of surgical techniques. Improvements are needed. 20 Brief Description and Objectives of the Invention The invention features wound-healing properties, high strength, durability, and absorbency. a surgical suture with enhanced antibacterial properties and environmental advantages and its It relates to the production method. The suture, which is the subject of the invention, is obtained in the following way: hemp. The fibers of walnut shells have the chemical formula 5-hydroxy-1,4-naphthoquinone. dyed with a brown pigment, coated with polylactic acid (PLA), more then dyed and PLA-coated fibers are woven together with five spinning machines The production of multifilaments, finally chlorhexidine diacetate, bay leaf oil, Coating with a mixture containing St. John's wort oil and melted beeswax. One aim of the invention is to obtain a suture with wound-healing properties. The invention is 30 The subject is sutures, biocompatible and biodegradable PLA's high drug delivery capacity. Modified St. John's wort oil with wound-healing effect thanks to 3 In addition, cannabis reduces bacterial buildup, scarring, and slows down healing. It supports healing because it inhibits healing. Also, chlorhexidine diacetate Bay leaf oil provides antibacterial and antioxidant effects. Another aim of the invention is to provide a suture that is easier to apply. Knot High-security hemp fibers are made from 5 different parts of walnut trees, including leaves, roots, bark, and husks. It has the chemical formula 5-hydroxy-1,4-naphthoquinone, which is naturally found in its parts. By staining it with a brown pigment, the thread can be easily seen during the operation. In addition, the multifilament structure of the beeswax-coated suture ensures a smooth passage. It provides this and because it has low friction, it minimizes tissue trauma and It reduces the risk of reaction formation. Thanks to its absorbable properties, it also removes foreign matter from the body. No objects remain. Additionally, the invention aims to provide a durable suture. To this end, the suture is designed... a natural fiber that provides high strength, durability and absorbency in its structure Hemp fiber is used. Additionally, it offers many extra benefits such as UV protection and anti-allergic properties. Hemp, which offers advantages, is coated with durable, highly processable PLA. The issue of suture durability is being taken to even further levels. Another aim of the invention is to obtain a more environmentally friendly suture with increased sustainability. Therefore, walnut shells, which are produced in large quantities but have very limited uses, are produced in a limited area. New uses for agricultural forestry waste are being identified. In addition, the PLA's 20 that can be obtained from renewable sources such as corn, starch and sugar beet Being a sustainable bioplastic material reduces the environmental impact of the invention. Explanation of the Figures Figure 1. Structure of the suture subject to the invention. Figure 2. Final sample 25 Figure 3. SEM image of five yarn samples produced from hemp fiber. Figure 4. Tensile test results of five yarn samples produced from hemp fiber (B1-B4: Five woven yarns produced from hemp fiber (main samples) 4 Figure 5. Five yarns produced from hemp fiber and a cotton yarn (Reference) sample. Comparison of tensile test results of the sample (B1: Produced from hemp fiber) Five-yarn knitted sample, P: Cotton yarn (reference) sample) Figure 6. FTIR graph of five yarn samples produced from hemp fiber (B1: Hemp) (Five knitted samples made from fiber) 5 Figure 7. Surgical thread (a) and patented coated suture (b) against S. aureus and E. coli. A graph showing the logarithmic rate at which bacteria decrease. Reference Numbers 1. Hemp suture 10 2. Polylactic acid (PLA) coating 3. Beeswax coating Detailed Description of the Invention The invention features a wound-healing property, high strength, durability, absorbency, 15 This relates to a surgical suture with enhanced antibacterial properties and environmental advantages. The surgical suture that is the subject of the invention; a. stained with 5-hydroxy-1,4-naphthoquinone obtained from walnut shells and five hemp fibers coated with polylactic acid (PLA) (2) multifilament hemp suture (1), 20 b. PLA coating (2) applied to the outer surface of hemp suture (1), c. 20-80% by weight chlorhexidine diacetate, 25-30% by weight bay leaf leaf oil, 20-50% by weight St. John's wort oil and 20-50% by weight It contains a beeswax coating (3) which contains beeswax in percentage. 25 In one application of the invention, the surgical suture that is the subject of the invention; a. stained with 5-hydroxy-1,4-naphthoquinone obtained from walnut shells and five hemp fibers coated with polylactic acid (PLA) (2) multifilament hemp suture (1), b. PLA coating (2) coated on the outer surface of hemp suture (1), 30 5 c. 8 mL chlorhexidine diacetate, 5 mL bay leaf oil, 5 mL St. John's wort oil and contains a beeswax coating (3) with 25 mL of beeswax. The invention features wound-healing properties, high strength, durability, and absorbency. a surgical suture with enhanced antibacterial properties and environmental advantages, and 5 of these It relates to the production method. The suture that is the subject of the invention is made from 5-hydroxy- obtained from walnut shells. Five fibers dyed with 1,4-naphthoquinone pigment and coated with polylactic acid (PLA) Hemp multifilaments obtained by knitting with a spinning machine and containing chlorhexidine. a beeswax coating containing diacetate, bay leaf oil and St. John's wort oil (3) It includes. 10 Hemp is a natural fiber that provides high strength, durability and absorbency, and is an annual fiber. It has very high efficiency. It also offers UV protection and anti-allergic properties. Surgical sutures are dyed with organic walnut shell dye, ensuring the thread adheres properly during the operation. This ensures easy visibility. Additionally, the silk filaments are visible after the dyeing process. The sutures are 15 by using air and chemicals to oxidize the dye inside. Sterilization is performed. The main by-products obtained from walnut fruit are the green husk. and a hard shell. The chemical composition of walnut shell fibers includes ash, lignin, hemicellulose, and It contains cellulose. It can also be used as a natural dyeing source, such as juglone. Juglone, Walnut trees naturally produce these substances in different parts such as leaves, roots, bark, and shells. It is a brown pigment found in the field. Today, it is used as an agricultural byproduct in green 20 The use of the shell is minimal. However, walnut shells have antioxidant and antimicrobial properties. Walnuts are used as a source of phytochemicals or natural compounds with specific properties. increasing the value of cultivation and finding new uses for agricultural waste It creates. Beeswax, as a natural lubricant, is inert to body fluids and is 25 It is resistant to sterilization conditions and is suitable for coating surgical sutures. Beeswax, certain saturated or unsaturated acids with high molecular weight, alcohols, and esters. It consists of a mixture of alkali esters (72%), free fatty acids (14%), The components are hydrocarbons (11%), free alcohols (1%), and other trace substances (2%). Beeswax has a density close to one (0.966). Its melting point is 62-64 °C. 30 PLA is biocompatible and biodegradable, and is naturally produced by the body. It is a material that can be broken down and absorbed. Therefore, implants and surgical sutures... 6 It is ideal for other medical devices and reduces the risk of allergic reactions or tissue rejection. They are strong and durable; this makes them suitable for orthopedic implants, dental materials, and surgical applications. This enables its use in load-bearing applications such as tools. PLA's processability And drug delivery capacity characteristics allow for the production of different medical devices and products. The hemp sutures described in the invention are of a multifilament structure and allow for easy passage through tissue. 5 It provides protection and has low friction, therefore it reduces tissue trauma and reaction. It reduces the risk of formation. Thanks to its absorbable properties, no foreign object remains in the body. And thanks to its durable structure, it provides knot security. The aforementioned beeswax The beeswax in the mixture provides surface smoothness, while St. John's wort oil has wound-healing properties. Chlorhexidine diacetate has antibacterial effects and bay leaf oil has antioxidant effects. 10 This is what the study results show. The surgical suture contains E. coli and S. aureus. It did not have antimicrobial activity against bacteria, but the coated thread sample... It reduced the growth of E. coli bacteria by 104 times and the growth of S. aureus bacteria by 102 times. It has been observed that it reduces the number of layers (Table 1). Table 1: Surgical thread, coated suture (patented invention) and control 15 Antimicrobial activity results of the sample The manufacturing method of the surgical suture that is the subject of the invention; a. Cleaning the bark of the hemp plant with a mechanical decorticator, outer 20 scraping away the meats and pectins, b. Washing the extracted fiber with tap water and air drying, c. 20% by weight of sodium hydroxide (NaOH) in the obtained crude fiber immersion in the solution and heating at 95°C for 120 minutes, d. then, washing with deionized water to remove NaOH residues and 25 Drying at 27°C, 7 e. The resulting threads are pulled out with tweezers to a thickness of 0.5 mm. sorting, f. Add 20-30 grams of walnut shell powder to 100 mL of distilled water at 80°C for 15 minutes. mixing throughout, g. Adding the fibers to this mixture and stirring at 80°C at 300 rpm for 60 minutes, 5 h. After the mixing process, the extracted fibers are cleaned with distilled water. drying, i. For the PLA impregnation process, 30 cm of 1.75 mm diameter PLA filaments are used. cut to length and sterilized with ethylene oxide gas, j. Melting PLA filaments at 170 °C, 10 k. dipping hemp fibers into molten PLA and holding them for 30-60 seconds, l. Removal of hemp fibers at the end of the soaking period, shaking off excess liquid. Removal of PLA and drying in a sterile environment, m. then, the dyed and PLA-coated fibers are woven with five spinning machines, n. knitted yarns containing 20-80% by weight chlorhexidine diacetate, 15 by weight 25-30% bay leaf oil, 20-50% St. John's wort oil by weight, and 35- A mixture containing 20-50% beeswax by weight, melted at 50°C. immersion in after the threads are allowed to solidify in the mixture for 30-60 seconds The process involves the steps of drawing the fibers. 20 8 References 1- Trott, AT (2012). Instruments, suture materials, and closure choices. Wounds and Lacerations, 82–94. https: / / doi.org / 10.1016 / b978-0-323-07418-6.00008-3 2- Dart, A.J., & Dart, CM (2011). Suture material: Conventional and stimuli responsive Comprehensive Biomaterials, 573–587. 5 https: / / doi.org / 10.1016 / b978-0-08-055294-1.00245-2 3- Suzuki, J. B., & Resnik, R. R. (2018). Wound dehiscence. Misch’s Avoiding Complications in Oral Implantology, 402–439. https: / / doi.org / 10.1016 / b978-0- 323-37580-1.00011-1 10

Claims

9 REQUESTS 1. Possessed of wound healing properties, high strength, and durability. A surgical product with enhanced absorbency, antibacterial properties, and environmental advantages. It is a suture and its characteristic is; 5 a. stained with 5-hydroxy-1,4-naphthoquinone obtained from walnut shells and five hemp fibers coated with polylactic acid (PLA) (2) multifilament hemp suture (1), b. PLA coating (2) applied to the outer surface of hemp suture (1), c. 20-80% by weight chlorhexidine diacetate, 25-30% by weight 10 bay leaf oil, 20-50% by weight of St. John's wort oil and by weight Beeswax coating containing 20-50% beeswax (3) It includes.

2. According to Claim 1, it is a surgical suture whose characteristic is; in an application of the invention. a. stained with 5-hydroxy-1,4-naphthoquinone obtained from walnut shells and 15 five hemp fibers coated with polylactic acid (PLA) (2) multifilament hemp suture (1), b. PLA coating (2) applied to the outer surface of hemp suture (1), c. 8 mL chlorhexidine diacetate, 5 mL bay leaf oil, 5 mL St. John's wort beeswax coating containing oil and 25 mL of beeswax (3) 20 It includes.

3. According to Claim 1, it is a surgical suture manufacturing method, characterized by the following features: a. the bark of the hemp plant with a mechanical decorticator cleaning, scraping away the outer layers and pectins, b. Washing the extracted fiber with tap water and air drying, 25 c. 20% by weight of sodium hydroxide (NaOH) in the obtained crude fiber immersion in the solution and heating at 95°C for 120 minutes, d. then washing with deionized water to remove NaOH residues. and drying at 27°C, e. Using tweezers, the resulting threads are cut into strips approximately 0.5 mm thick. 30 by pulling and removing, f. Add 20-30 grams of walnut shell powder to 100 mL of distilled water at 80°C for 15 minutes. mixing throughout, 10 g. The fibers are added to this mixture and baked at 80°C at 300 rpm for 60 minutes. mixing, h. After the mixing process, the extracted fibers are cleaned with distilled water. drying, i. For the PLA impregnation process, 30 cm of 1.75 mm diameter PLA filaments are used. cut to length and sterilized with ethylene oxide gas, j. Melting PLA filaments at 170 °C, k. dipping hemp fibers into molten PLA and holding them for 30-60 seconds, 1. Removing the hemp fibers at the end of the soaking period, shaking off the excess. Removal of molten PLA and drying in a sterile environment, 10 m. then, dyed and PLA-coated fibers with five spinning machines knitting, n. 20-80% by weight of chlorhexidine diacetate in knitted yarns, 25-30% bay leaf oil by weight, 20-50% St. John's wort oil by weight oil and 20-50% by weight of beeswax melted at 35-50°C 15 immersion in the mixture containing After the threads are allowed to solidify in the mixture for 30-60 seconds then the fibers are pulled It includes the steps of the process. 20

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