Aronia Fruit Nutraceutical Syrup: Production Method, Analytical Bioactive Profile, and Use as a Protective Dietary Supplement for MCF-7 Breast Cancer.

Abstract

The invention relates to aronia tea containing aronia berry powder and water; aronia berry nutraceutical syrup containing chlorogenic acid, (-)epigallocatechin gallate (ECCG), quercetin 3-O-ß-galactoside form; and the method of its production. The invention also includes the use of aronia berry nutraceutical syrup as a dietary supplement to aid in the treatment of breast cancer.

Description

1 TARIFF ARONIA FRUIT NUTRACEUTICAL SYRUP, PRODUCTION METHOD, ANALYSIS BIOACTIVE IF PROFILE and MCF-7 have protective effects against breast cancer. USE AS A SUPPLEMENTARY FOOD SUPPLEMENT Technical Area The invention relates to aronia berry nutraceutical syrup and its production method. The invention specifically refers to aronia tea containing aronia berry powder and water; chlorogenic acid, (- 10 Aronia berries containing epigallocatechin gallate (ECCG), Quercetin 3-O-β-galactoside form. The invention relates to nutraceutical syrup and its production method. The invention also includes nutraceuticals derived from aronia berries. the syrup can also be used as a food supplement to aid in the treatment of breast cancer. It includes. State of the Art 15 Aronia (chokeberry) fruit is a multi-stemmed, distinctive fruit belonging to the Rosaceae family. It is a deciduous shrub-type plant during certain periods. Aronia is predominantly found in North America. It grows in this region and is also cultivated to a significant extent in Eastern Europe. The genus Aronia includes three subspecies: A. arbutifolia (L.) Pers – red. aronia berry; A. prunifolia (Marsh.) Rehd. – purple aronia berry; A. melanocarpa 20 (Michx.) Ell. – black aronia berry. In the literature, a study conducted by Kara S. et al. in 2018 discussed Aronia berries. and Cancer Research: Application of Aronia melanocarpa to epithelial ovarian cancer Immunocytochemical and apoptotic effects on the cell line SKOV-3 were revealed 25 This study focuses on one of the deadly gynecological cancer types. The effects of aronia berries on epithelial ovarian cancer have been investigated, and Aronia In vitro investigation of the effects of Melanocarpa on the SKOV-3 cell line. The aim was to achieve Aronia Melanocarpa concentrations of 400 μg / ml, 300 μg / ml, and 200 μg / ml. μg / ml, 150 μg / ml, 100 μg / ml, 75 μg / ml, 50 μg / ml, 25 μg / ml, 10 μg / ml, 5 μg / ml, 1 μg / ml 30 2 It was applied in this manner and at intervals of 24 hours, 48 ​​hours, and 72 hours. In the relevant research, Aronia To determine the cytotoxic effects of Melanocarpa on CA cells, the MTT test is effective. Five doses were selected and their effects on apoptosis were investigated using the Tunnel method. This active substance targets the PI3K, mTOR, p38, and MAPK signaling pathways in tumor cells. Its effect on the pathway was determined by immunocytochemical (ICC) methods. As is known, 5 Like many types of cancer, ovarian cancer initially responds to chemotherapy drugs. While it is effective in the short term, drug resistance develops in the long term, and treatment fails. In recent years, antioxidants have been used in combination with chemotherapeutic drugs to suppress resistance. It is also recommended for treatment. In the study conducted, cytotoxicity As a result of the analyses, the suppressive concentration (IC50) value of Aronia melanocarpa was found to be 10. It was found to be 50 μg / ml. Compared to the control group of Aronia Melanocarpa... In the study, which also determined apoptotic effects, this activity was observed with PI3K / mTOR and p38 MAPK. It has been found that this occurs via signaling pathways. Aronia treatment When combined with the antichemarterapeutic agent paclitaxel, the most effective dose It was stated that there was 50 μg / ml Aronia and 0.02 μM paclitaxel. 15 In a study conducted by Handelanda M. et al. in 2014, black aronia (Aronia) The juice of (Melanocarpa) reduces the incidence of urinary tract infections. It has been found that urinary tract infections (UTIs) are a significant problem in nursing homes. It is seen as a problem and it is reported that the mainstay of treatment is antibiotics. The increasing prevalence of resistance of uropathogens to antimicrobial agents has been observed. To prevent recurrent urinary tract infections, specific nutrients (cranberry; (cranberries, etc.) has increased the tendency. In the relevant pilot study, conducted in 6 nursing homes In studies, black aronia juice was administered to selected subjects for six months. The study involved administering a placebo drink to nursing home residents over a three-month period. Then, for the next 3 months, either aronia juice (group A) or the opposite (group B) was given. 25 The fruit juice studied contained B-type procyanidins, anthocyanins, and chlorogenic acids. Including a total phenolic content equivalent of 715 mg gallic acid, per 100 ml-1 (ml It was characterized as (above -1). Daily aronia juice intake was observed in subjects in Group A. 156 mL per subject in Group A (n = 110) and 89 mL per subject in Group B (n = 126). It has been determined that urinary tract infections occurred 30 times during the study period in question. It accounts for 55% of all medically treated infections. The results are obtained from urine. an immediate increase in the frequency of urinary tract infections (UTIs) or in total antibiotic use However, it has been shown that there is no decrease in aronia fruit over the next 3 months. During the application of the water, a decrease in urinary tract infections was observed in both groups. This subsequently resulted in a decrease in antibiotic use as well. The incidence of UTIs was 35 in Group A. 3 It showed a 55% reduction, and a 38% reduction in Group B. In other infections or No changes in prophylactic treatments have been reported. In a study conducted by Bermudez-Soto MJ et al. in 2007, a polyphenol-rich substance was found. CaCO-2 levels in colon cells after repeated use of aronia juice in the diet In the study where the change was determined, 5 was related to tumor suppressor carcinoembryonic antigen. The regulation of cell adhesion molecules has been investigated. As is known, developing In many countries, colon cancer is one of the leading causes of cancer deaths. Consumption of polyphenol-rich red fruits and berries can help with colon health. There is data suggesting that it reduces cancer risk, and the mechanisms involved are still being investigated. It is not clear enough. The study included 10 patients with subconfluent colon carcinoma. The response of Caco-2 cells to repeated exposure was investigated, and subjects were given the exposure for 4 days. during the period, 2 hours a day at a subtoxic dose containing mixed polyphenols Aronia juice was administered. In the study conducted, physiological conditions were observed. under the supervision of aronia berries, they were subjected to in vitro gastric and pancreatic digestion. It is stated that and vitality, effects on proliferation and cell cycle 15 It is stated that genes have been identified and respond to aronia treatment. changes in expression using affimetric oligonucleotide microarrays It is stated that it was screened. In the study, its expression was found in early adenomas and tumor suppressor carcinoembryonic antigen, which is known to be reduced in most carcinomas bound cell adhesion molecule 1 (CEACAM1), both mRNA and protein 20 regulated by the treatment performed at these levels (as also shown by flow cytometry analysis) It is stated that the results of cell viability and cell proliferation have been revealed. It was placed. No viability was observed in the control cells (only a small percentage, about 10%). (proliferation was observed) it was stated. Compared to untreated cells, Exposure of Caco-2 cells to 2% and 5% digested aronia juice resulted in 25 days of ingestion, respectively. It has been reported to have resulted in approximately 40% and 70% inhibition. Relevant research... within this context, Caco-2 cell proliferation through aronia treatment Inhibition has been associated with G2 / M cell cycle arrest. In a study conducted by Kedzierska M. et al. in 2013, it was found that black aronia extract had 30 In another study that examined its biological activity against breast cancer, Plasma in invasive breast cancer after stages I, II, III, and IV of chemotherapy. The levels of 3-nitrothyrosine in proteins are compared to plasma proteins of patients with invasive breast cancer. According to this study, it was found to be significantly higher. In this study, melanocarpa... 4 The commercial extract Aronox (Agropharm Ltd., Poland; batch no. 020 / 2007k) was tested in vitro. In the developed model, the extract of A. melanocarpa (50 µg / ml, 15 min, 37oC), from invasive breast cancer patients after stages I, II, III, IV of chemotherapy (47 Plasma proteins obtained from women with invasive breast cancer (age range 43-72, mean 55.1) It has been found to reduce modifications. 5 during different phases of treatment. The commercial extract, tested through examinations of plasma samples taken from patients, antioxidant enzymes (superoxide dismutase, glutathione peroxidase, glutathione reductase) It has been found to reduce the modification of enzymes. Patent application number TR2021 / 018303 concerns chemical composition and antioxidant activity. Aronia concentrate, rich in B vitamins (B2, B3, B6, B9, B12, B5), C10 vitamin E, selenium, zinc and mineral supplements (sodium, calcium, chloride, (potassium, magnesium, fluoride, iron, copper, sulfate and bicarbonate) present together It relates to the method of producing functional beverages. Patent application number TR2021 / 020098 concerns C60 and mineral water. with aronia (Aronia melanocarpa) or elderberry (Sambucus nigra L.) concentrate / extract 15 by making it beneficial, without a bitter taste, and strengthening the immune system. It is related to mineral water and water and concentrate that it provides. However, in the current technique, aronia tea contains chlorogenic acid, (-)epigallocatechin gallate (ECCG), A dietary supplement containing quercetin in its 3-O-β-galactoside form, used to aid in breast cancer treatment. There is no aronia berry nutraceutical syrup available that can be used as a supplement. 20 In conclusion, due to the negative aspects described above and the current solutions being the subject of discussion... Due to its shortcomings, an improvement is needed in the relevant technical field. It has been made. The purpose of the invention The invention was created by drawing inspiration from existing situations and addressing the aforementioned drawbacks. 25 It aims to solve the problem. The primary purpose of the invention is to synthesize Chlorogenic acid, (-)epigallocatechin gallate (ECCG), Quercetin 3-O- A dietary supplement containing β-galactoside, used as an aid in the treatment of breast cancer. The aim is to identify a usable aronia berry nutraceutical syrup and its production method. The antioxidant activity and antioxidant phenolic profile of the product subject to the invention were determined in detail by HPLC. (High-Pressure Liquid Chromatography) and Q-TOFF-MS (Quadrupole Time of Flight) The major active bioactives were determined by Mass Spectrometry. On selected cancer cell lines (SKBR-3 breast cell lines) Antiproliferative and anticarcinogenic effects have been detected. 5 Analytical determinations revealed that the phenolic acids are gallic acid, as well as flavanols (catechins). epigallocatechin, catechin, chlorogenic acid, hydroxycinnamic acid, neochlorogenic acid, vanillic acid, syringic acid, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, t-sinapic acid acid, rosemarinic acid; rutin (quercetin-3-glucoside) as flavonols and glycosides, kaempferol-3-rutinoside, quercitrin (quercetin-O-glycoside), quercetin, astragalin 10 (kaempferol-O-glucoside), kaempferol has been identified. Aronia nutraceutical supplement syrup SPAF contains chlorogenic acid, the major phenolic compound. (ChA) was determined to be 321.56 mg / 100g, 493.20 mg / 100g, and 202.38 mg / 100g, respectively. It has been observed that the values ​​are in mg / 100g. Brewed for 3 min, 5 min, 10 min, 15 min. In aronia teas, ChA levels were 90.28 mg / 100g, 95.63 mg / 100g, and 188.42 mg / 15g, respectively. It was determined to be mg / 100 g and 195.33 mg / 100 g, and has high antioxidant properties. It provides (p < 0.05). The major flavonol found was quercetin, and aronia The fruit, its powder, and nutraceutical syrup contained 634.57 mg / 100g and 542.80 mg / 100g respectively in SPAF. The values ​​were determined to be mg / 100g and 620.25 mg / 100g. Quercetin is extremely potent. It is predicted to be an antioxidant and to provide antioxidant stability to the related products (p< 20). 0.05). Quercetin and chlorogenic acid at a concentration of 5-100 μM and incubation for 48 hours. Consequently, concentration-dependent SKBR-3 levels in breast cancer cells It has been determined that 100 μM quercetin optimally inhibits its proliferation. It reduced SKBR-3 cells by 56.25% and increased cell proliferation by 43.75%. It appears to inhibit SKBR-3 at the level of chlorogenic acid, followed by SKBR-3. reduced proliferation to 62.5% and cell proliferation to 37.50% It has been found to inhibit at the level of only 10 μM of catechin bioactive. It reduced SKBR-3 cells by 65.66% at that concentration and increased cell proliferation. It appears to be able to inhibit by 34.34% (p < 0.05). All chromatographic, chemical 30 And cytological data reveal that chlorogenic acid, one of the major polyphenolic compounds, Quercetin and catechin are highly effective antacarcinogenic substances. It is observed that our developed aronia products have innovative antioxidant and antiproliferative properties. It is anticipated that these products can be used in the health sector as food supplements. 6 The nutraceutical supplement product (SPAF) MCF-7 obtained within the scope of the study. Cell culture application was performed using cells and antiproliferation The results have been presented. Antioxidant activity and antiproliferation values. Anticarcinogenic properties have been identified in our selected product. To fulfill the purposes described above, the invention is an antioxidant, antiproliferative, and 5-in-100-degree invention. It is a nutraceutical syrup with anticarcinogenic properties containing aronia berries; aronia berry powder and aronia tea containing water; Chlorogenic acid, (-)epigallocatechingallate (eccg), Quercetin 3-O-β- It contains galactose form. An application of the invention; 90-92% aronia tea by weight; 1.8-2% chlorogenic acid, 3-4% (- )epigallocatechin gallate (ECCG) contains 1.5-2% quercetin 3-O-β-galactoside form. 10 An application of the invention; 92% aronia tea by weight; 2% chlorogenic acid, 4% (- ) Contains epigallocatechin gallate (ECCG), 2% Quercetin 3-O-β-galactoside form. To fulfill the purposes described above, an invention called Aronia berry nutraceutical was created. This is a syrup production method that includes the following steps: i. Sorting and washing the aronia berries 15 ii. Drying the washed aronia berries. iii. Grinding dried aronia berries into powder, iv. Obtaining aronia tea by brewing aronia fruit powder in water, v. Aronia tea; Chlorogenic acid, (-)epigallocatechingallate (eccg), Quercetin 3-O- Mixing of the β-galactoside form, 20 vi. Pasteurization of the obtained aronia fruit nutraceutical syrup. vii. Vacuum bottling of pasteurized aronia berry nutraceutical syrup. The method described in the invention involves vacuuming until a maximum moisture level of 4.5% is reached. Drying is done. The dried aronia berries are ground into powder up to 0.5 mesh. 25 It is processed into powder. For aronia tea, a concentration of at least 3 g / 100 ml of aronia is required. The fruit powder is steeped in hot water for 3-15 minutes and then cooled to room temperature. 7 The invention describes a nutraceutical syrup made from aronia berries as a food supplement to aid in the treatment of breast cancer. It also includes its use in supplement production. The structural and characteristic features and all the advantages of the invention are given in the figures below. This becomes clearer thanks to the detailed explanation written with references to these figures. This will be understood as such, and therefore the evaluation will also be based on these forms and detailed explanations. 5 This should be done taking that into consideration. Ways to Help Understand the Discovery Figure 1 shows the gallic acid, chlorogenic acid (5-caffeoylquinic acid), in aronia powder. hydroxycinnamic acid, neochlorogenic acid (3-caffeoylquinic acid), vanillic acid, syringic acid, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, t-sinapic acid and rosemarinic acid 10 The chromatograms are shown side-by-side. Figure 2 (ac), Gallic acid in aronia teas brewed for 5 min, 10 min and 15 min, Chlorogenic acid (5-caffeoylquinic acid), hydroxycinnamic acid, neochlorogenic acid (3- co-determined forms of caffeoylquinic acid), vanillic acid, syringic acid, and rosemarinic acid. Chromatograms are shown. 15 Figure 3 (ab) shows the Rutin (Quercetin-3-) in aronia tea and Nutraceutical Syrup (SPAF). glycoside), Kaempferol-3-rutinoside, Quercitrin (Quercetin-O-glycoside), Quercetin, Astragalin (Kaempferol-O-glucoside) and Kaempferol have been determined side-by-side. Chromatograms are shown. 20 Figure 4 (ab), Factors in the inhibition of proliferation in SKBR-3 mammary cell lines. The phenolic compounds and their inhibition levels are observed. Figure 5 (ae), Individual 25 Effective in Antiproliferation of SKBR-3 Breast CA Lines. Phenolic acids and flavonoids. Figure 6 (ac), Aronia Tea-10 min in Antiproliferation of SKBR-3 Breast CA Lines Effects of Tea and Nutraceutical Syrup (SPAF) Samples. Figure 7 (ac), Aronia Tea Extract in Antiproliferation of SKBR-3 Breast Cancer Lines and the Effects of Aronia Fruit Extract Samples. 8 Detailed Description of Find This detailed explanation describes the Aronia nutraceutical syrup (SPAF) and the preferred ingredient of the invention. the structures that have been established are solely for the purpose of better understanding the subject. It is explained. Table 1. Subject of the invention: Aronia nutraceutical syrup (SPAF) content 5 Content Preference amount Available by Weight amount Aronia tea 92% 90-92% Chlorogenic acid 2% 1.8-2% (-)epigallocatechin gallate (ECCG) 4% 3-4% Quercetin 3-O-β- galactoside form 2% 1.5-2% In the studies conducted as part of the invention, selected aronia berries were brought to the laboratory. Following the introduction of these products and subsequent appropriate drying processes, the following products were manufactured: Aronia Powder Production: Aronia (Aronia melanocarpa sp. Viking) fruit sorting and washing processes 10 Then the drying process was carried out in a tunnel-type dryer. Along the tunnel on the rail. aronia berries on a conveyor belt system that operates on the principle of movement. During this period, the product was dried at 45°C until a moisture level of 3% was reached; each product... The product is dried by placing 15 kg of product per m2 area and is processed in 2 stages. This was carried out. In the first stage, a higher temperature was applied, while in the second stage, a lower temperature of 15 was applied. A heat treatment was applied. At the outlet end, there were 6 successive wagons carrying the dried product. The dried final products obtained from the car system are mixed with a sanitized steel blade mixer. (Waring, USA) It is ground into powder to a size of 0.5 mesh; vacuum processed. It is integrated into the packaging system and used in analyses and in the production of other products. It has been preserved in this way. 20 Aronia Tea Production: 9 Aronia (Aronia melanocarpa sp. Viking) fruit after sorting and washing processes Then the drying process was carried out in a vacuum dryer. In the vacuum drying system Aronia berries were incubated for 6 hours on the first day at 48.5°C and 100 mbar pressure, Completed at 80.7°C under 230 mbar pressure. Pre-grinding process performed on day 2. Our aronia berries were placed at 120°C and 230 mbar pressure for 3 hours and 20 minutes. starting from 84.3°C, under 230 mbar pressure, and until a humidity level of 4.14% is reached. It has been dried; the product is dried by placing 5 kg of product in each drying tray. This was carried out in stages. In the first stage, more heat was applied, while in the second stage... Lower temperatures were applied in this stage. The dried final products are sanitized. Grinding to a powder (10) size down to 0.5 mesh with a steel blade mixer (Waring, USA). It has been transformed into this form; it is placed in a vacuum packaging system for analysis and other products. It has been preserved for use in production. The subject of the invention is the production of Aronia nutraceutical syrup (SPAF): The aronia tea product obtained in the previous step was evaluated in sensory tests. A selected brewing time quality has been established. Brewing times for 3 min, 5 min, 15 min. Brewing times were determined as 1 minute, 10 minutes, and 15 minutes. 3 grams of water were used in the brewing process of the tea samples. Sample and 100 ml bottled water with pH = 7.50 were used. Brewing time was the most preferred. After selecting the desired tea product (10-minute brewing time), it is brewed and then cooled to room temperature. Cooled to 24±1 ºC, the combination of two phenolic acids and one flavonol described in Table 1. It is fortified with antioxidants and mixed in a batch system and baked at 45ºC for 20 days. It has been pasteurized. The resulting product has been vacuum-bottled. Analysis Results: Table 2. Total Phenolic Compound Contents in Aronia and Aronia Products. GAE / 100 gr GAE: Gallic acid equivalent 25 As seen in the table, the total phenolic content of aronia teas varies depending on brewing times. In substance determination, 150.23±1.01 (mg GAE / 100 g) (n=3) for 3 min brewing, 5 min brewing 341.86±3.23 (mg GAE / 100g) (n=3) for 10 min brewing; 696.92±67.23 (mg GAE / 100g) For 15 min brewing, 787.92±130.24 mgGAE / 100 ml (n=3) was determined. Aronia tea has a higher total phenolic content after brewing for 15 minutes. The level of total phenolic content of aronia tea after 15 minutes of brewing has been found to be... The high level of phenolic compound transfer increases as the brewing time increases. This is due to the increase. Table 3. Total Anthocyanin Contents in Aronia and Aronia Products 10 Cye: Cyanide Equivalent As seen in the table, the total amount of aronia tea at different brewing times... Anthocyanin content determination: 21.28±0.47 (mgCyE / 100g) (n=3) for 3 min brewing, 5 min For brewing: 34.56±0.12 (mgCyE / 100g)(n=3), for 10 min brewing: 78.14±9.08 15 (mgCyE / 100g)(n=3), 87.92±6.16 (mgCyE / 100g)(n=3) was detected for 15 min brewing. It has been observed that the total phenolic content of aronia tea increases after a 15-minute brewing time. High levels of total phenolic compounds were found. Aronia tea, brewed for 15 minutes, contains... The amount of phenolic compounds found to be high increases with brewing time. This is due to the increased transmission. 20 11 Table 4. DPPH Antioxidant Activity Values ​​in Aronia and Aronia Products. DPPH· (1,1-Diphenyl-2-picrylhydrazyl radical; C18H12N5O6) antioxidant analysis 5 It is commonly used to evaluate the antioxidant activity of different compounds. It is a quick, basic method used. As shown in the table, total anthocyanin, DPPH, and antioxidant levels vary at different brewing times. In the activity determination, the DPPH antioxidant activity for 3 min brewing was 96.55±5.72 (mg). GAE / 100 g) (n=3), 179.86±18.63 (mg GAE / 100g) (n=3) for 5 min brewing 10 min 10 For brewing: 186.20±20.42 (mg GAE / 100 g) (n=3) for 15 min brewing. 22.56±1.58189.72±10.55 (mg GAE / 100 g) (n=3) was determined. Aronia tea 15 min. The longer the brewing time, the higher the total DPPH antioxidant activity. It has been found that brewing aronia tea for 15 minutes increases the antioxidant activity of DPPH. The level of emergence is due to the increased transfer of substances as the brewing time increases. 15 It originates from. 12 Table 5. Aronia Fruit, Aronia Tea Infusion and Aronia Nutraceutical Syrup (SPAF) Organic Acid Levels Malic acid content is 4.31 ± 0.56 (g / kg) for fruit and 2.34 ± 0.02 for tea after 3 minutes of brewing. (g / kg), 2.89 ± 0.05 (g / kg) for 5 min brewing, 2.55 ± 0.03 (g / kg) for 10 min brewing, 15 5 2.40 ± 0.05 (g / kg) for min. brewing and 4.46 ± 0.12 (g / kg) for nutraceutical syrup (SPAF) It has been determined. The citric acid content is 0.72 ± 0.05 (g / kg) for fruit and 0.50 ± 0.02 for tea after 3 minutes of brewing. (g / kg), 0.52 ± 0.02 (g / kg) for 5 min brewing, 0.40 ± 0.05 (g / kg) for 10 min brewing, 15 0.42 ± 0.01 (g / kg) for min brewing and 0.65 ± 0.02 (g / kg) for nutraceutical syrup (SPAF) 10 It has been determined. The quinic acid content is 3.98 ± 0.07 (g / kg) for fruit and 2.55 ± 0.05 for tea after 3 minutes of brewing. (g / kg), 2.20 ± 0.02 (g / kg) for 5 min brewing, 1.85 ± 0.05 (g / kg) for 10 min brewing, 15 1.67 ± 0.01 (g / kg) for min brewing and 5.22 ± 0.05 (g / kg) for nutraceutical syrup (SPAF) It has been determined. 15 Table 6. Phenolics of Aronia Fruit, Powder, Teas and Nutraceutical Syrup (SPAF). Retention Time (RT) and Quantitative Amounts of Acids (mg / 100g). 13 Table 6 continues. Aronia fruit, Powder, Teas and Nutraceutical Syrup (SPAF) Retention Time (RT) and Quantitative Amounts of Phenolic Acids (mg / 100g). 14 The Turkish aronia included in the research, which involved qualitative and quantitative analyses. The fruit is aronia. Viking origin aronia powder, aronia tea and specific formulations. The aronia nutraceutical product (SPAF) contains phenolic acids, flavanols (catechins), and flavonols. The compounds were qualitatively and quantitatively determined using Q-TOFF-Mass Spectrometry. Determined by HPLC-DAD. 5 Gallic acid and chlorogenic acid (5-caffeoylquinic acid) are phenolic acids. hydroxycinnamic acid, neochlorogenic acid (3-caffeoylquinic acid), vanillic acid, syringic acid, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, t-sinapic acid, and rosemarinic acid using High Pressure Liquid Chromatography (HPLC) to determine the same baseline side-by-side. The values ​​were determined and their quantitative measurements were carried out. 10 The Turkish aronia berries examined were aronia var. Viking-origin aronia powder, aronia tea, and The specially formulated aronia nutraceutical product contains phenolic acids ranging from 0.52 to 493.20 mg / 100g. It was detected within this range. Figure 1 shows the gallic acid, chlorogenic acid (5-afeoylquinic acid), 15 in aronia powder. hydroxycinnamic acid, neochlorogenic acid (3-caffeoylquinic acid), vanillic acid, syringic acid, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, t-sinapic acid and rosemarinic acid The chromatograms are shown side-by-side. Figure 2 shows the gallic acid content in aronia teas brewed for 5, 10, and 15 minutes. Chlorogenic acid (5-caffeoylquinic acid), hydroxycinnamic acid, neochlorogenic acid (3- co-determined forms of caffeoylquinic acid), vanillic acid, syringic acid, and rosemarinic acid. Chromatograms are shown. In specially formulated aronia nutraceutical syrup (SPAF), Chlorogenic acid (5-caffeoylquinic acid), neochlorogenic acid (3-caffeoylquinic acid), quinic It has been determined as acid major. 25 The research included Turkish aronia berries, Viking-origin aronia powder, aronia tea, and In specially formulated aronia nutraceutical syrup (SPAF), regarding phenolic acid compounds According to the results obtained from the chromatograms, fruit, powder, teas and Retention time (RT) of acids in nutraceutical syrup (SPAF), and 30 Quantitative amounts (mg / 100g) of phenolic acids have been determined. (mg / 100g) aronia berry powder, used in teas with different brewing times and nutraceuticals It has been determined for the syrup (SPAF). 16 Gallic acid content is 2.23 mg / 100g in fruit, 5.37 mg / 100g in powder, and 3 minutes in tea product. 0.52 mg / 100g after brewing, 0.76 mg / 100g after 5 minutes of brewing, 1.12 mg / 100g after 10 minutes of brewing. (mg / 100g), 1.33 (mg / 100g) was detected after 15 minutes of brewing. Epigallocatechin levels are 9.35 mg / 100g in fruit, 7.52 mg / 100g in powder, and 3 mg / 100g in tea product. 8.45 (mg / 100g) in 1 minute brewing, 13.32 (mg / 100g) in 5 minutes brewing, 13.32 (mg / 100g) in 10 minutes brewing The concentration was determined to be 19.26 (mg / 100g), and 20.44 (mg / 100g) after 15 minutes of brewing. For catechins, 34.46 mg / 100g in fruit, 35.67 mg / 100g in powder, and 3.10 mg / min for tea product. 38.16 mg / 100g after brewing, 41.55 mg / 100g after 5 minutes of brewing. The concentration was determined to be 30.78 mg / 100g, and 30.96 mg / 100g after 15 minutes of brewing. Chlorogenic acid levels were 321.56 mg / 100g in fruit, 493.20 mg / 100g in powder, and in tea product. For 3 minutes of brewing: 90.28 mg / 100g, for 5 minutes: 95.63 mg / 100g, for 10 minutes: 15 188.42 mg / 100g in brewing, 195.33 mg / 100g in 15 min brewing, and nutraceuticals For the syrup (SPAF), 202.38 mg / 100g was detected. Hydroxycinnamic acid levels are 4.17 mg / 100g in fruit, 6.26 mg / 100g in powder, and in tea product. For 3 minutes of brewing: 3.08 mg / 100g, 5 minutes of brewing: 3.44 mg / 100g, 10 minutes: 20 mg / 100g The concentration was determined to be 4.69 mg / 100g after brewing and 4.90 mg / 100g after brewing for 15 minutes. Neochlorogenic acid levels were 274.35 mg / 100g in fruit, 186.56 mg / 100g in powder, and in tea product. For 3 minutes of brewing: 42.55 mg / 100g, for 5 minutes: 58.03 mg / 100g, for 10 minutes... infusion 67.62 (mg / 100g), 15 min infusion 65.58 (mg / 100g) and nutraceutical syrup 25 SPAF was determined to be 123.38 (mg / 100g). Vanillic acid content is 30.12 mg / 100g in fruit, 38.67 mg / 100g in powder, and 3 minutes in tea product. 10.96 mg / 100g after brewing, 11.56 mg / 100g after 5 minutes of brewing. 15.67 mg / 100g was detected, and 12.78 mg / 100g after 15 minutes of brewing. 30 Syringic acid was detected at 7.63 mg / 100g in the fruit and 4.12 mg / 100g in the powder. Kaftarik Caffeic acid was detected at 1.88 mg / 100g in the fruit and 3.24 mg / 100g in the powder. The concentration was determined to be 6.49 mg / 100g in the fruit and 6.12 mg / 100g in the powder. 35 17 p-Coumaric acid is 3.27 mg / 100g in the fruit and 4.91 mg / 100g in the powder; ferulic acid is present in the fruit. 2.15 mg / 100g was detected in the sodium molecule, and 2.33 mg / 100g in the powder. t-Cinapic acid is 1.52 mg / 100g in fruit, 1.59 mg / 100g in powder, and 3 minutes for tea product. 0.55 mg / 100g after brewing, 1.02 mg / 100g after 5 minutes of brewing, 1.25 mg / 100g after 10 minutes of brewing. (mg / 100g), 1.22 (mg / 100g) was detected after 15 minutes of brewing. Rosemarinic acid is 2.58 mg / 100g in fruit, 1.59 mg / 100g in powder, and 3 minutes for tea product. 0.60 mg / 100g after brewing, 0.62 mg / 100g after 5 minutes of brewing, 0.89 mg / 100g after 10 minutes of brewing. (mg / 100g), after 15 minutes of brewing, it was determined to be 0.54 (mg / 100g). 10 Figure 3 shows the Rutin (Quercetin-3-) in aronia tea and Nutraceutical Syrup (SPAF). glycoside), Kaempferol-3-rutinoside, Quercitrin (Quercetin-O-glycoside), Quercetin, Astragalin (Kaempferol-O-glucoside) and Kaempferol have been determined side-by-side. Chromatograms are shown. 15 Rutin (Quercetin-3-glucoside) is found in aronia berries at 182.46 mg / 100g and in its powder at 109.56 mg / 100g. mg / 100g, for tea: 70.13 mg / 100g after 3 minutes of brewing, 72.52 mg / 100g after 5 minutes of brewing, 10 minutes of brewing... infusion 113.40 mg / 100g, 15 min infusion 128.66 mg / 100g and nutraceutical syrup (SPAF) 72.76 mg / 100g was detected. 20 Kaempferol-3-rutinoside is found in aronia berries at 87.25 mg / 100g and in the powder at 73.58 mg / 100g. For tea, brewing for 3 minutes yields 96.72 mg / 100g, brewing for 5 minutes yields 94.26 mg / 100g, brewing for 10 minutes yields... 60.56 mg / 100g, 48.93 mg / 100g after 15 minutes of brewing, and 143.92 mg / 100g in nutraceutical syrup (SPAF). mg / 100g was determined. 25 Quercitrin (Quercetin-O-glucoside) is found in aronia berries at 45.22 mg / 100g and in its powder at 32.16 mg / 100g. mg / 100g, for tea: 40.36 mg / 100g after 3 minutes of brewing, 44.58 mg / 100g after 5 minutes of brewing, 10 minutes of brewing... The levels were determined to be 28.62 mg / 100g in brewed tea and 35.27 mg / 100g in brewed tea after 15 minutes. Quercetin is found in aronia berries at 634.57 mg / 100g, in the powder at 542.80 mg / 100g, and in the tea at 3 minutes. 307.20 mg / 100g in brewing, 328.96 mg / 100g in 5 minutes brewing, 380.56 in 10 minutes brewing mg / 100g, brewed for 15 minutes, 387.25 mg / 100g, and nutraceutical syrup (SPAF) 620.25 mg / 100g has been determined. 35 18 Astragalin (Kaempferol-O-glucoside) is found in aronia berries at 59.30 mg / 100g and in its powder at 54.56 mg / 100g. mg / 100g, for tea: 42.52 mg / 100g after 3 minutes of brewing, 38.45 mg / 100g after 5 minutes of brewing, 10 minutes of brewing... The levels were determined to be 63.56 mg / 100g in brewed tea and 60.40 mg / 100g in brewed tea after 15 minutes. Kaempferol is found in aronia berries at 38.3 mg / 100g, in the powder at 35.62 mg / 100g, and in the tea at 3 minutes to 5 minutes. 29.37 mg / 100g in 5 minutes, 27.12 mg / 100g in 5 minutes, 28.35 mg / 100g in 10 minutes, 15 minutes The levels were detected as 26.27 mg / 100g in oral decoction and 22.86 mg / 100g in nutraceutical syrup (SPAF). It has been done. Cellular Cytotoxicity Analyses: 10 MTT, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) is a tetrazolium salt, It is a specific agent for the succinate-dehydrogenase enzyme in the mitochondria of living cells. In the MTT method, succinate-dehydrogenase, located in the mitochondria of living cells, is used. The enzyme breaks down the tetrazolium ring of the MTT dye, resulting in the water-insoluble formazan. It forms salts. The value measured spectrophotometrically in the MTT method is 15 This indicates the metabolic activity of cells in culture, and this value represents living organisms. It is related to the number of cells. As proliferation increases, it is due to the formation of formazone salt. The absorbance value is increasing. In the study, aronia berry-ethanol in the extract, powdered tea-ethanol extract, aronia tea infusion (brewed for 10 minutes and MTT 20 in tea infusion (with a sieve size of 0.5 µm) and in the nutraceutical specialty product SPAF (syrup). According to the results, SKBR-3 had a concentration- and time-dependent effect on mammary cell lines. Depending on the context, possible cytotoxic and antiproliferative effects have been identified. Figure 4 shows the agent that inhibits proliferation in SKBR-3 mammary cell lines. The phenolic compounds and their inhibition levels are observed. 25 Figure 5 shows the individual SKBR-3 lines that are effective in the antiproliferation of breast cancer cells. Phenolic acids and flavonoids are observed. Figure 6 shows the effect of aronia tea (10 min brewing) on ​​the antiproliferation of SKBR-3 breast cancer lines. and effects are observed in nutraceutical syrup (SPAF) samples. In studies conducted on SKBR-3 cell lines, catechin, quercetin, and chlorogenic acid were used. acid, quinic acid and rutin at a concentration of 5-100 μM and after 48 hours of incubation Dose-dependent inhibition of cell viability / cell growth in SKBR=3 cells 35 19 It has been found to increase cell proliferation and antiproliferation. %Cell proliferation, Routine < The acid levels were determined to be in the following order: Chlorogenic acid < Quercetin < Quinic acid < Catechin. In our research conducted on SKBR-3 cell lines, quercetin and chlorogenic acid... After incubation of the acid at a concentration of 5-100 μM for 48 hours, the concentration... 5 It optimally inhibits the proliferation of SKBR-3 cells in a dependent manner. It is observed that when the highest bioactive concentration of 100 μM is applied; Quercetin reduced SKBR-3 cells by 56.25% and increased cell proliferation. It appears to inhibit SKBR-3 by 43.75%, followed by chlorogenic acid. reduced proliferation to 62.5% and cell proliferation to 37.50% It appears to inhibit it at the level of 10. As can be seen in Figure 6, catechin bioactive is inhibited at the level of 10. It reduced SKBR-3 cells by 65.66% at a concentration of only 10 μM, and the cells It appears to be able to inhibit proliferation by 34.34%. Figure 7 shows the effect of aronia tea extract on the antiproliferation of SKBR-3 breast cancer lines. and the effects of aronia fruit extract samples are observed. 15 In research conducted on SKBR-3 breast cancer cell lines, a bioactive standard Following the investigation of the antiproliferation of the active ingredients, aronia berry-ethanol in its extract, in powdered tea-ethanol extract, in aronia tea infusion (brewed for 10 minutes and (in tea infusion with a sieve size of 0.5 μm) and in the nutraceutical specialty product SPAF (syrup), MTT 20 According to the results, SKBR-3 had a concentration- and time-dependent effect on mammary cell lines. Depending on the concentration, possible cytotoxic and antiproliferative effects have been identified (0.5-10 μg / ml). SKBR-3 concentration and dose dependence of the product after 48 hours of incubation. in cells, cell viability / cell growth is inhibited and antiproliferation occurs. The level has been determined. 25 aronia tea infusion brewed for 5 minutes at a concentration of 5 μg / ml and incubated for 48 hours. Ultimately, it reduced SKBR-3 cells to 71.86% and increased cell proliferation. It was found to inhibit by 28.14%, while at the same concentration (5 μg / ml) The nutraceutical supplement syrup SPAF reduced SKBR-3 proliferation by 74% and 30 It appears to inhibit cell proliferation by 26% (Figure 56). 10 μg / ml Concentration and 48-hour incubation result in 10-minute brewed aronia tea. Deminin and the nutraceutical supplement syrup SPAF reduced SKBR-3 cells by 36.50% and 36.50%, respectively. reduced to 49%, therefore cell levels were 63%, 50% and 51% respectively. It has been found to inhibit proliferation, resulting in extremely potent antiproliterative samples. It has been shown that they are. The extraction methodology of aronia tea and aronia fruit extracts has been explained. In the research conducted on SKBR-3 breast cancer cell lines, MTT 5 According to the results, SKBR-3 had a concentration- and time-dependent effect on mammary cell lines. Depending on the context, cytotoxic and antiproliferative effects have been observed. Aronia tea extract was obtained at a concentration of 0.5-10 μg / ml and after a 48-hour incubation period. Depending on the aronia fruit extract doses, 10 SKBR-3 cells The level of antiproliferative activity has been determined. Aronia tea extract (aronia tea ethanol extract), at a concentration of 10 μg / ml and 48 After an hour of incubation, it reduced SKBR-3 cells to 93.75%, and the cells It was determined that it inhibited proliferation by 6.25%, while aronia fruit extract inhibited it by 15%. (Aronia fruit ethanol extract) at a concentration of 5 μg / ml and incubation for 48 hours. As a result, CA cells were reduced to 88.5%, therefore 11.5% antiproliferative It has been determined that it has an effect. Cytotoxic effects were statistically significantly higher at a concentration of 25 μg / ml over 48 hours compared to the control group. The results were determined as (p≤0.05) for aronia tea brewed for 10 minutes and SPAF syrup. The samples were found to exhibit highly effective antiproliferative properties. In the study, aronia tea infusion (10 min) was used on human SKBR-3 breast cancer cells. (in its brewed form) is extremely effective compared to aronia ethanol extract and 25 It has been found to have cytotoxic effects (Figure 6, Figure 7) and may have therapeutic properties. It is anticipated that SPAF, a nutraceutical supplement syrup derived from aronia, will also have an important role. Because it exhibits a highly effective antiproliferative effect, it is considered a therapeutically effective supplement. It is suggested that it can be used. Turkish aronia berry (Aronia melanocarpa Viking) is used in health products such as aronia powder. SPAF produces aronia tea and a specially developed nutraceutical aronia syrup. This has been achieved; basic product profiles and total phenolic content in existing innovative products. contents, total anthocyanin amounts, antioxidant activity levels and High High-Resolution Mass Spectrometry by Pressurized Liquid Chromatography (HPLC) 35 21 Detailed data can also be obtained via Quadrupole Time of Flight Mass Spectrometry (Q-TOFF-MS). Phenolic bioactive profiles have been determined. The taste and overall quality of the products are also improved through sensory analysis and quality rating tests. Acceptability standards have been established, taking all assessments into consideration. Aronia-based health products to be commercialized have been identified and are sectorally... The products to be evaluated were selected by selecting SKBR-3 cancer cells. Antiproliferative and anticarcinogenic effects on cell lines have been investigated. The moisture content in aronia berries, aronia tea, and aronia powder ranges from 2.0% to 74.34%. It was determined that the sugar levels varied between 8.19% and 27.97%. It was determined (p < 0.05). Total phenolic content (TFM) of aronia berries and developed aronia products. It is seen to be rich in terms of. Aronia berry, powder form and aronia 15 Nutraceutical syrup SPAF products contain 1294.86 – 3946.49 mg Gallic Acid Equivalent (GAE). It was determined that it was in the range of / 100 g (p < 0.05). Brewed for 3 min, 5 min, 10 min, 15 min. In aronia teas, sensory information was determined to be between 150.23 and 787.92 mg GAE / 100 g. According to the results obtained from the tests, aronia tea brewed for 10 minutes has a better taste and drinkability. Its quality has stood out and it has received a score of 9.0 on a scale. Aronia, brewed for 10 minutes, 20 The TFM in the tea was determined as 696.92 ±67.23 mg GAE / 100 g. Nutrosotic aronia The SPAF syrup was determined to contain 1294.86 ±59.31 mg GAE / 100 g (p < 0.05). Anthocyanins, which are antioxidant color compounds, are found in aronia berries, powder, 3 min, 5 In aronia teas brewed for 10, 15 minutes, and in SPAF syrup, 21.28 – 1658.81 mg 25 The cyanidin equivalent (CyE) is at levels of / 100 g, and aronia is brewed for 10 minutes. Their teas showed 78.14±9.08 mg CyE / 100 ml (p< 0.05). DPPH The Antioxidant Activity (AA) values ​​of Trolox in the studied products were 96.55-291.34 µmol. Its equivalent (TE) has been determined to be in the range of / 100 g. In aronia tea brewed for 10 minutes. 186.20±20.42 µmol TE / 100 g and 189.72±10.55 µmol TE / 100 g in tea brewed for 15 min. It was determined that the amount of AA was statistically significant in teas brewed for 10 and 15 minutes. No significant difference was found in terms of nutraceutical syrup SPAF (p < 0.05). AA was found to have a value of 190.52±12.35 µmol TE / 100g in the relevant products. High antioxidant activity is observed. 35 22 Aronia berries, aronia tea, aronia powder, and nutraceutical aronia supplement syrup. In SPAF, the organic acids identified are malic, citric, and quinic acid, and malic acid is the primary acid. Citric acid at 2.34-4.46 g / kg, quinic acid at 0.40-0.72 g / kg, and quinic acid at 2.20-5.22 g / kg. It has been detected within this range. In particular, quinic acid has been reported to have antioxidant effects. and the content in our products has been found to be at a good level. 5 Aronia berry, powder, aronia tea and nutraceutical supplement syrup SPAF have a phenolic profile. Determination was performed by HPLC and Q-TOFF MS. Phenolic acids and flavanols (catechins) Retention times (RT) of gallic acid, epigallocatechin, catechin, chlorogenic acid, hydroxycinnamic acid, neochlorogenic acid, vanillic acid, syringic acid, caftaric acid, caffeic acid 10 The acids are, in order, p-coumaric acid, ferulic acid, t-sinapic acid, and rosemarinic acid; the same Determination was performed side-by-side on the same baseline in the chromatogram (Figure 33, Figure 34). Aronia berries, powder, aronia tea and nutraceutical supplement syrup in SPAF, 90.28-493.20 mg / 100g chlorogenic of major phenolic acids and flavanols (catechins). acid (ChA); 42.55-274.34 mg / 100g neochlorogenic acid (NCA), 30.97-35.67 mg / 100g 15 Catechin (C), 10.96-38.67 mg / 100g vanillic acid (VA), 7.52-20.44 mg / 100g epigallocatechin It is observed that (EGC) is the most important phenolic compound, chlorogenic acid (p < 0.05). Aronia berry, powder, nutraceutical syrup SPAF contains 321.56 mg / 100g, respectively. The findings were 493.20 mg / 100g and 202.38 mg / 100g. 3k,5min, In aronia teas brewed for 15 minutes, the chlorogenic acid levels were 90.28 and 20%, respectively. The values ​​were determined as mg / 100g, 95.63 mg / 100g, 188.42 mg / 100g, and 195.33 mg / 100g. It is observed to have significant antioxidant properties (p < 0.05). Retention times (RT) of flavonols and flavonol glycosides in rutin (quercetin-3- glycoside), kaempferol-3-rutinoside, quercitrin (quercetin-O-glycoside), quercetin, astragalin 25 (kaempferol-O-glycoside), is in the kaempferol sequence. Aronia berry, powder, aronia. The major flavanols and glycosides in SPAF tea and nutraceutical supplement syrup contain 307.20- 634.57mg / 100g quercetin, 70.13-182.46mg / 100g routine (quercetin-3-O-glycoside), 48.93- 143.92 mg / 100g kaempferol-3-rutinoside and 38.45- 63.56 mg / 100g It was determined that it was astragalin (kaempferol-3-O-glucoside) ((p< 0.05). 30 The major flavonol, quercetin, is found in aronia berries, their powder, and the nutraceutical syrup SPAF. respectively 634.57 mg / 100g; 542.80 mg / 100g, and 620.25 mg / 100g It has been observed that the following results were obtained from teas brewed at different times: 3k, 5min, 10min. In aronia teas brewed for 15 minutes, quercetin levels were 307.20 mg / 100g and 35%, respectively. 23 It was determined as 328.96 mg / 100g; 380.56 mg / 100g; and 387.25 mg / 100g. It is observed that quercetin is an extremely powerful antioxidant and is used in related products. It is predicted to provide antioxidant stability (p < 0.05). In the study, aronia berry-ethanol extract, powdered tea-ethanol extract, and aronia 5 in tea brewing (tea brew brewed for 10 minutes and with a sieve size of 0.5 μm) and nutraceutical According to MTT results in the special product SPAF (syrup), SKBR-3 mammary cell lines Potential cytotoxic and antiproliferative effects depending on concentration and time. Effects have been detected. In our research conducted on SKBR-3 cell lines, quercetin and chlorogenic acid... After incubation of the acid at a concentration of 5-100 μM for 48 hours, the concentration... It optimally inhibits the proliferation of SKBR-3 cells in a dependent manner. It is observed that when a high bioactive concentration of 100 μM is administered, quercetin SKBR-3 reduced breast cancer cells by 56.25% and cell 15 It appears to inhibit proliferation by 43.75%, followed by chlorogenic The acid reduced SKBR-3 breast cancer cell proliferation by 62.5%. and it has been shown to inhibit cell proliferation by 37.50%, which is extremely high. These are important results. The catechin bioactive substance was present in SKBR-3 at a concentration of only 10 μM. It reduced cell proliferation by 65.66% and breast cancer cell proliferation by 34.34%. It appears that it can inhibit it. All chromatographic, chemical and cytological data reveal that the major polyphenolic Chlorogenic acid, quercetin, and catechin are highly effective antacarcinogens. These substances are seen to be innovative antioxidant and the developed aronia products are 25 antiproliferative products can be used as food supplements in the health field. It is anticipated.

Claims

24 REQUESTS 1. Aronia berries with antioxidant, antiproliferative, and anticarcinogenic properties. It is a nutraceutical syrup with the following properties: aronia tea containing aronia berry powder and water; Chlorogenic acid, (-)epigallocatechingallate (eccg), Quercetin 3-O-β-galactoside 5 It includes the form.

2. Nutraceutical syrup containing aronia berries, conforming to Claim 1, with the characteristic of having 90-92% by weight. Aronia tea contains 1.8-2% chlorogenic acid, 3-4% (-)epigallocatechin gallate (ECCG), and 1.5-%.

2. Quercetin contains the 3-O-β-galactoside form.

3. Nutraceutical syrup with aronia berries, conforming to Claim 1, with the characteristic of having 92% by weight. Aronia tea contains 2% chlorogenic acid, 4% (-)epigallocatechin gallate (ECCG), and 2%... Quercetin contains the 3-O-β-galactoside form.

4. Which of the above claims is a method for producing nutraceutical syrup from aronia berries? and its characteristic is; i. Sorting and washing the aronia berries 15 ii. Drying the washed aronia berries. iii. Grinding dried aronia berries into powder, iv. Obtaining aronia tea by brewing aronia fruit powder in water, v. Aronia tea; Chlorogenic acid, (-)epigallocatechingallate (eccg), Quercetin 3-O- Mixing of the β-galactoside form. 20 5. This method complies with Claim 4 and its characteristic is that it reaches a maximum humidity level of 4.5%. This involves vacuum drying.

6. This method complies with Claim 4 and its characteristic is; 0.5% of dried aronia berries. It is the process of grinding the material until it becomes a powder.

7. This method complies with Claim 4 and its characteristic is; 0.5 25 of dried aronia berries. It is the process of grinding the material until it becomes a powder.

8. The method compliant with claim 4 is characterized by a concentration of at least 3 g / 100 ml. Steep aronia berry powder in water for 3-15 minutes and let it cool to room temperature. It is cooling.

9. The method complies with Claim 4, and its characteristic is; the resulting aronia berry nutraceutical This involves pasteurizing the syrup. 5 10. The method is in accordance with claim 8, and its characteristic is; Pasteurized aronia berries. It is the vacuum bottling of nutraceutical syrup.

11. Aronia berry nutraceutical syrup conforming to one of claims 1-3 for breast cancer Its use is in the production of food supplements to aid in treatment.