AN ALLERGEN-FREE FRAGRANCE FORMULATION

Abstract

The invention relates to an allergen-free fragrance formulation developed as an alternative to the compound 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-gamma-2-benzopyran (galaxolide), which was added as a new allergen fragrance raw material by the European Union regulation number 2023 / 1545 concerning fragrance allergens, especially for use in the cosmetics and fragrance sectors.

Description

1 TARIFF AN ALLERGEN-FREE FRAGRANCE FORMULATION Technical Area The invention is intended for use in the cosmetics and fragrance industry, and has been registered with the European Union. Regulation number 2023 / 1545 regarding fragrance allergens includes new allergen fragrance raw materials. 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-gamma-2- added as An allergen-free fragrance developed as an alternative to the compound benzopyran (galaxolide). It is related to the formulation. 10 State of the Art Cosmetics as defined by European Union Parliament Regulation No. 1223 / 2009 15 new allergen fragrance raw materials have been added to the 26 allergen fragrance raw materials used in the products. With the addition and updating of regulation 2023 / 1545, the number of allergen-specific fragrance raw materials has increased to 83. The following allergen has been removed from the list: 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8- The compound hexamethylcyclopenta-gamma-2-benzopyran (galaxolide) has also been added [1]. The compound in question has the CAS number 1222-05-5 and is used in cosmetics, particularly for babies. 20 personal care products made for people with sensitive skin, including these products Their use will be restricted. Thus, new allergen-free products will be introduced in these areas. Formulations need to be improved. In the current technical context, according to the updated cosmetics regulation mentioned, cosmetic products... (Shampoo, soap and indirectly most products related to skin sensitivity and babies 25 (This also applies to) hypoallergenic products are manufactured. Hypoallergenic products also... no allergenic content or below the limits specified in this regulation It is necessary. In products that rinse with water, such as shampoo and soap, a maximum of one allergen per product is required. The limit is 100 ppm. If the allergen level exceeds 100 ppm, it must be indicated on the product label. Similarly, for products that do not require rinsing (perfume, baby oil, baby lotion, wet wipes, etc.), the limit is 30. The maximum amount for each allergen is 10 ppm. The same applies above 10 ppm. It must be stated on the product label. Some product manufacturers, however, use 10 ppm and 100 ppm. Regardless of concentration, it requires the allergen content to be 0 ppm. For this reason... The development of new formulations that are non-allergenic and have a long-lasting fragrance is necessary. 2 Application number WO2021043585A1, which is included in the known state of the art, concerns improved fragrance. It belongs to the field of perfume oils with a profile, and within this field, it is characterized by its composition and fragrances. It deals with methods and formulations developed to alter fragrance notes. Another known case of the art involves application number US11844854B2, concerning perfume 5. To produce consumer products including raw materials, perfumes, and perfume delivery systems, and It relates to the methods of use. Explanation of the Figures Figure 1. Galaxolide and two substitutes for the non-allergenic fragrance formulation that is the subject of this invention. A representative view of a graph showing the performance test results of baby lotion essence. Figure 2. Galaxolide and two substituted non-allergenic fragrance formulations that are the subject of this invention. A representative graph showing the performance test results of baby wet wipe fragrance. appearance. 15 Figure 3. Galaxolide and two substituted non-allergenic fragrance formulations that are the subject of this invention. A representative view of a graph showing the performance test results of a perfume essence. Brief Description of the Invention The present invention eliminates the aforementioned disadvantages and the related technical to provide new advantages to the field and especially for use in the cosmetics and fragrance industry; The 1,3,4,6,7,8- ingredients have been added as new allergen raw materials by the European Union. to the compound hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-gamma-2-benzopyran (galaxolide) It relates to an alternative, allergen-free fragrance formulation. 25 The invention developed a new formulation for non-allergenic fragrance formulations. allergen-free products that comply with European Union Standards are provided. It is being developed. More specifically, the purpose of the invention is to enter into force on July 26, 2023. The European Union's list of allergen-free fragrance raw materials to be used in cosmetic products is 30. allergens to be substituted for galaxolide raw material, as stated in the regulation. The goal is to provide a fragrance that does not contain any scent. The invention is used in cosmetics such as baby shampoo where allergenic components are undesirable or restricted. 3 an alternative formulation that is free of allergens in products and is also a non-cosmetic product and other products for babies such as baby detergent, baby lotion, and baby fabric softener. An alternative, allergen-free formulation is also obtained in the product groups. The invention relates to 5 products such as baby shampoo, baby concentrated fabric softener and baby liquid detergent. Testing fabric washing processes in terms of scent persistence and in conjunction with these product groups. Sensory analyses of baby lotion and baby wipes products revealed the scent. It has been proven that it remains at an equivalent level to the galaxolide raw material. Furthermore, it is used in perfume essences. Sensory analyses, including those conducted using various methods, also prove its persistence on human skin. Thus, the invention results in a long-lasting and non-allergenic fragrance formulation. 10 Detailed Description of the Invention Examples of arrangements can be further detailed by referring to the accompanying explanations below. It is described as such. However, regulations can be formulated in different forms and 15 This should not be interpreted as being limited to the regulations mentioned here. Instead, this example... regulations ensure that this statement is complete and its scope is fully understood by technically qualified individuals. It has been provided for transmission in this manner. Below are a few examples of compound trade names for the formulation that is the subject of this invention. 20 The names in question can be increased, decreased, or other names can be included, according to the literature. It can be used. In practice, the invention may be used with the trade names mentioned in the description. It is not limited. The invention is classified under the new 25 standards of IFRA (International Fragrance Association) within the European Union. 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8- added as allergen essence raw material Developed as an alternative to the compound hexamethylcyclopenta-gamma-2-benzopyran (galaxolide) It relates to an allergen-free fragrance formulation; - 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H-inden-4-one (cashmeran), - cyclohexadec-8-en-1-one (globanone), 30 - 1-propanol, 2-(1-(3,3-dimethylcyclohexyl)ethoxy)-2-methyl-,1-propanoate (helvetolide), - dipropylene glycol, - acetic acid, (1-oxopropoxy)-1-(3,3-dimethylcyclohexyl) ethyl ester (romandolide), - oxacycloheptadec-10-en-2-one (ambrettolide), 4 - It contains 1,4-dioxacycloheptadecane-5,17-dione (ethylene brassylate). The fragrance formulation described in this invention is preferred in one of its applications; - 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H- in the range of 0.1-1.5% by weight inden-4-on (cashmeran), 5 - cyclohexadec-8-en-1-one (globanone) in a concentration of 0.1-1.5% by weight. - 0.1-1.5% by weight of 1-propanol, 2-(1-(3,3-dimethylcyclohexyl)ethoxy)-2- methyl-,1-propanoate (helvetolide), - dipropylene glycol in the range of 50-90% by weight, - Acetic acid, (1-oxopropoxy)-1-(3,3-10) in concentrations of 0.5-3% by weight. (dimethylcyclohexyl) ethyl ester (romandolide), - oxacycloheptadec-10-en-2-on (ambrettolide) in concentrations ranging from 0.5-3% by weight. - 1,4-dioxacycloheptadecane-5,17-dione (ethylene) in a concentration of 15-35% by weight. It contains brassylate. The names and CAS numbers used for the fragrance formulation covered by the invention are given in Table 1. is given; Table 1. Formulation Contents Subject to the Invention Trade Name Chemical Name CAS Number Cashmeran 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H- inden-4-on 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H- inden-4-one 33704-61-9 Globanone cyclohexadec-8-en-1-on cyclohexadec-8-en-1-one 3100-36-5 Helvetolide 1-propanol, 2-(1-(3,3-dimethylcyclohexyl)ethoxy)-2- methyl-, 1-propanoate 1-propanol, 2-(1-(3,3-dimethylcyclohexyl)ethoxy)- 2-methyl-, 1-propanoate 141773-73-1 Dipropylene glycol Mixture of 1,2-dipropylene glycol ether isomers 1,2-dipropylene glycol ether, mixture of isomers 25265-71-8 Romandolide acetic acid, (1-oxopropoxy)- 1-(3,3- (dimethylcyclohexyl) ethyl ester acetic acid, (1-oxopropoxy)-, 1-(3,3- dimethylcyclohexyl)ethyl ester 236391-76-7 Ambrettolide oxacycloheptadek-10-en-2-on oxacycloheptadec-10-en-2-one 28645-51-4 Ethylene brassylate 1,4-dioxacycloheptadecane-5,17-dione 1,4-dioxacycloheptadecane-5,17-dione 105-95-3 Galaxolide belongs to the group of polycyclic musk compounds and is generally a fragrance from the musk family. It is a fragrance chemical. In terms of scent profile, musk is floral, powdery, sweet, clean, and somewhat... It can be described as woody. The allergen-free formulation subject to the invention is galaxolide 5 The compound was made to provide a fragrance profile equivalent to all these fragrance profiles. It was developed as a result of experiments and sensory analyses. The invention concerns a formulation containing cashmeran and a musk and floral fragrance from the musk fragrance family. A profile is obtained. In addition, the fragrance strength of the fragrance formulation is increased. 10 It provides. The formulation subject to this invention contains globanone, which is a musk from the musk family, with a floral, clean and sweet scent. A scent profile is obtained. The formulation subject to the invention contains dipropylene glycol, used as a solvent in the formulation. It is used. The prepared formulation is diluted to a certain ratio to determine its fragrance intensity. This allows it to be brought to an equivalent level with galaxolide. Although the compound romandolide does not belong to the polycyclic musk group in terms of its structure, it has a scent similar to 20 It has a characteristic olfactory profile similar to polycyclic musk compounds. This is generally It can be described as musky and powdery. Thus, the invention has a musky and powdery scent. A profile is obtained. The formulation subject to the invention contains ambrettolide, a sweet and soapy musk from the musk family. A fragrance profile is obtained. In addition, the fragrance longevity of the fragrance formulation is determined. 6 It ensures that it is increased. The invention describes a formulation containing ethylene brassylate with a musk and powdery fragrance profile. It is obtained. Furthermore, this compound is the most cost-effective and suitable for perfume production. It is a musk-class fragrance chemical that increases longevity. For this reason, it is included in the formulation as a 5. After dipropylene glycol, this fragrance chemical is used in the highest concentration. The formulation developed with this invention is for the fragrance and consumer product industry where fragrances are used. It is used in baby care products; for example, baby detergent, baby liquid detergent, baby fabric softener, baby wipes, baby shampoo, baby soap, baby 10 lotion and all similar products are allergen-free personal care products for people with sensitive skin types. or all non-personal cleaning and care products, baby care products and all including tissues, wet or dry wipes, detergents, fabric softener, shampoo and soap Cleaning and care products for external use in the cosmetics industry that are allergen-free. It is used in the sector. 15 The formulation that forms the basis of the invention emerges as a result of many trials. Each In a formulation trial, the compound galaxolide was used in the invention, and the formulation in question had an olfactory quality. The above-mentioned contents and ratios were compared after the aforementioned experimental stages. This is revealed. In a sample experimental study conducted for the invention; 20 substitutes were used instead of galaxolide. The fragrances to be used in the formulation will be: baby shampoo, baby liquid detergent, baby In 6 separate categories: concentrated fabric softener, baby wipes, baby lotion, and perfume. The compound called galaxolide, found in the fragrance in each product category, has been evaluated. Instead, a non-allergenic fragrance formulation, the subject of the invention, has been used at the same concentration. In addition, galaxolide and 25 galaxolide substitutes were also found in 6 separate consumer product categories. The formulation subject to the invention is evaluated comparatively, the details of which are given below under subheadings. This is shown in the following experimental studies. The “non-allergenic” substances mentioned in the experimental studies below are shown in this form. The term "substitute formulation" refers to the fragrance formulation that is the subject of the invention. a. Fragrance 30 created to evaluate the scent performance in baby shampoo. formulation To evaluate the fragrance performance of baby shampoo, a sample containing 25% galaxolide was used. According to the following fragrance formulation; galaxolide and allergen at a concentration of 25% by weight. 7 Two separate essences containing a substitute formulation, which is not the subject of the invention, have been prepared. The relevant components The contents and percentages are shown in Table 2. Table 2. Baby Shampoo Fragrance Components and Proportions. Baby Shampoo Fragrance Ingredients (by weight, %) Dimethyl anthranilate 0.200 Phenyl acetic aldehyde 10% DPG 0.200 Ethyl vanillin 0,300 Ethyl maltol 0,500 Cassis base 0,500 Amber core 0,500 Allyl cyclohexyl propionate 1,000 Benzaldehyde 1,000 Allyl heptanoate 1,000 Maltol 5 pea 1,000 Isoamyl acetate 1,000 Oxane %1 DPG 1,000 Strawberry furanone %15 PG 1,000 Cassis base-345F 1,000 Nerolin bromelia 2,000 Gamma decalactone 3,000 Frambinone 3,000 Phenyl ethyl alcohol 3,000 Aldehyde c-16 6,000 Verdox / OTBCH acetate 10,000 Dihydro myrcenol 12,000 Diethyl malonate 12,000 Dipropylene glycol 13,800 Galaxolide 25,000 Toplam 100,000 Hazırlanan iki esans, standart parfümsüz bir bebek şampuanına, %1 konsantrasyonunda This was applied. In the performance test of the two prepared essences, one of the two 250 mL beakers was used. 8 2 grams of galaxolide-containing essence; the other beaker also contains a non-allergenic substitute, the subject of the invention. 2 grams of the essence containing the formulation was also added, weighed on a precision balance. To both beakers The glass was filled with hot water to approximately 35°C, bringing the volume to 250 mL. Using a stirring rod, mix the water in both beakers until the mixture becomes homogeneous. This was done. Equal amounts of real hair by weight were added to the water in both beakers. 5 The hair, after being immersed in hot water for approximately 30 seconds, was removed from the beakers. Galaxolide The subject formulation is the sensory and non-allergenic substitute of the fragrance containing the ingredient. The evaluation in terms of performance tests was carried out by 10 perfumers and fragrance analysts. This was done with a panel team of 10 people. After the processes listed above, galaxolide containing Using the original fragrance as a reference, a non-allergenic substitute formulation containing the fragrance is used as follows: 10 compared; - For scent diffusion in shampoo, galaxolide and non-allergenic substitute formulation. Baby shampoos containing these fragrances should be sniffed from a distance of approximately 2 cm. They were compared sensorially. - The scent disperses in wet hair after shampooing, containing galaxolide and 15 non-allergenic substances. by using baby shampoos that contain substitute formulations and fragrances After washing the hair in the prepared warm water, while it is still wet, brush it from a distance of approximately 2 cm. They were compared sensorially by smelling. - The scent's dispersion in dry hair after shampooing may be due to galaxolide and non-allergenic substances. Using baby shampoos that contain substitute formulations and fragrances can increase the risk of death. Hair washed in the prepared hot water is dried, and then, while dry, approximately 2 cm long. They were sensorially compared by smelling them from a distance. A panel of 10 people evaluated the essence containing galaxolide using a scoring system from 1 to 5. 25 containing allergen-free substitute formulations, referencing the baby shampoo used. It was compared to baby shampoo that uses fragrance. The scent mentioned in the points above. According to the evaluation criteria, the evaluation results of the 10-person panel team are shown in Table 3. It is shown. Table 3. Baby Shampoo Rating System Evaluation Results 30 Essence The smell in shampoo spread Scent of shampoo hair washed with spread (wet) Scent of shampoo hair washed with spreading (dry) 9 Fragrance containing galaxolide 4.77 4.77 4.00 Non-allergenic substitute essence containing formulation 3.44 3.77 3.44 As can be seen from the table, the use of baby shampoo containing 25% galaxolide is recommended. The fragrance developed for this purpose has been prepared in two different ways. In the first, it contains 25% galaxolide, and in the second... However, instead of galaxolide, there is a 25% non-allergenic substitute formulation. Both essences... The remaining 75% consists of the same raw materials. These two essences are 5 The purpose of the comparative evaluation is to use the same concentration instead of galaxolide. The question is how much the scent is affected when a substitute formulation is used. Scoring is done via a sensory test. In other words, it was done by smelling. In the scoring, "3 points" is the threshold value, and therefore, the invention... The substitute formulation must also receive at least 3 points. The scent in the shampoo The average score given by the panelists to the fragrance containing galaxolide for its diffusion was 4.77, while the substitute received a score of 10. The score for the essence containing the formulation was 3.44. It was substituted because it received a score above 3. The formulation has delivered the desired properties. b. Created to evaluate the fragrance performance of baby concentrated fabric softener. fragrance formulation 15 To evaluate the fragrance performance of baby concentrated fabric softener, use 10% According to the following fragrance formulation containing galaxolide; at a concentration of 10% by weight. galaxolide and two separate essences containing a non-allergenic substitute formulation, which is the subject of this invention. It has been prepared. The relevant component contents and ratios are shown in Table 4. 20 Table 4. Ingredients and Proportions of Baby Concentrate Fabric Softener Essence. Baby Concentrate Fabric Softener Fragrance Ingredients By weight % ratio Rosalva 0.030 Lime oxide 0.030 Phenyl ethyl acetate 0.050 Eugenol 0.050 Lilestralise sel 0.050 Para-cresyl methyl ether 0.080 Okoumal 0.100 Aldehyde c-11 undecylenic 0,100 Borneo 0.100 Methyl octine carbonate 0.150 Methyl benzoate 0.200 Aldehyde ISO C-11 0.200 Fenchol 0.200 Phenyl acetaldehyde 50% 0.200 1-terpinen-4-ol 0.250 Lyrasub 0.400 Methyl beta-naphthyl ketone 0.750 Cinnamic alcohol 0.800 Benzyl propionate 0.800 Benzyl salicylate 0.800 Aldehyde C-12 MNA 1,000 Isoraldeine 70 1,300 Phenyl ethyl alcohol 1,300 Coumarin 1,500 Heliotropex N 1,500 Musk ketone 2,000 Benzyl acetate 2,000 Anisaldehyde ex paracresol 2,500 Linalool 3,000 Lysmeral base 3,000 Hexyl cinnamic aldehyde alpha 3,500 Rosalin 3,500 Cyclacet 4,000 Terpineol 10,000 Galaxolide 10,000 Diethyl phthalate 44,560 Total 100,000 The two prepared essences were added to a standard baby concentrate fabric softener at a concentration of 1.5%. This was implemented. For the performance test of the two prepared essences; two separate containers of the same brand and model were used. 11 put laundry of approximately the same weight but different textures into the washing machine's drum. The same number of pieces of fabric of equal size were placed in the material. With both essences. 35 g of the prepared baby concentrated softeners were weighed. Weighed essences in laundry The liquid was poured onto the fabrics inside the machine's hopper. 40°C temperature and 1000 rpm. In the cycle, the rinsing process was carried out for 22 minutes. After the process was completed, the fabrics were washed for 5 minutes. They were removed from the machines. The fragrance containing galaxolide and the fragrance containing a non-allergenic substitute formulation, which is the subject of this invention. Evaluation in terms of sensory and performance tests, by perfumers and fragrance analysts. This was done with a panel team of 10 people. After the processes listed above, galaxolide 10 Using the fragrance reference as a reference, the following fragrance formulation contains a non-allergenic substitute: They were compared in this way; - Baby fabric softener made with galaxolide-containing fragrance is referenced as galaxolide. Baby formula made with the same essence but containing a non-allergenic substitute formulation. The fabric softener bottles were smelled simultaneously, and both products were sensorially evaluated on a 15-degree angle. They have been compared. - After washing in the washing machine, the smell of both products will linger while wet. The persistence of the scent on fabrics was compared sensorially. A panel of 20 people evaluated the essence containing galaxolide using a scoring system from 1 to 5. non-allergenic substitute formulation based on the baby concentrate fabric softener used. It was compared with a baby concentrate fabric softener that uses the essence contained above. According to the odor evaluation criteria specified in the articles, a panel team of 10 people The evaluation results are given in Table 5 below. Table 5. Results of the Baby Softener Scoring System Evaluation. Essence The smell of the baby concentrated in fabric softener the spread of Scented baby concentrate with fabric softener from washing fabrics then on the wet fabric spread and persistence Scented baby concentrate with fabric softener from washing fabrics then on the dry cloth spread and persistence Scent power Scent power Scent power 5 containing galaxolide 4.8 3.8 12 essence Non-allergenic substitute formulation containing essence 3.8 3.6 2.9 As can be seen from the table, this is a baby concentrate fabric softener containing 10% galaxolide. The fragrance developed for use has been prepared in two different ways. The first contains 10% galaxolide, The second one contains a 10% non-allergenic substitute formulation instead of galaxolide. Both essences... The remaining 90% consists of the same raw materials. These two essences are 5 The purpose of the comparative evaluation is to substitute galaxolide with the same concentration. It is about how much the formulation affects the smell when used. Scoring is done through a sensory test. This was done by sniffing. In the scoring, "3 points" is the threshold value, and therefore substitution is used. The formulation must also receive at least 3 points. The scent should be baby concentrate. The average score given by the panelists to the fragrance containing galaxolide for its diffusion in the fabric softener was 5 out of 10. However, the score for the essence containing the substitute formulation was 3.8. It received a score above 3. The substitute formulation has provided the desired properties. c. Fragrance created to evaluate the scent performance of baby liquid detergent. formulation 15 To evaluate the fragrance performance of baby liquid detergent, use 10% galaxolide. According to the following fragrance formulation containing; galaxolide at a concentration of 10% by weight and Two separate essences containing a non-allergenic substitute formulation were prepared. The component contents and ratios are shown in Table 6. 20 Table 6. Fragrance Components and Proportions in Baby Liquid Detergent. Fragrance Components in Baby Liquid Detergent By weight % ratio Benzaldehyde 0.040 Ethyl vanillin 0.050 Eucalyptol natural 0.050 Damascone delta 0.070 Galbascone 0.080 13 Aldehyde C-12 Lauric 0.100 Florhydral 0.100 Camphor 0,100 Para-cresyl methyl ether 0,100 Rose oxide 90-10 hc 0,100 Alcohol c-6 0,100 Phenyl ethyl methyl ether 0,100 Heptoxime pcas 0,100 Isoeugenol 0,150 Ambermax %50 dowanol TPM 0,150 Coumarin 0,200 Vertoliff 0,200 Styrallyl acetate 0,300 Eugenol 0,350 Diphenyl oxide 0,500 Hexyl acetate 0,500 Floralozone 0,500 Musk ketone 0,500 Tonalid 0,500 Undecavertol 0,800 Geraniol ultra 0,850 Verdox / OTBCH acetate 0,850 Phenyl ethyl acetate 1,000 Phenyl ethyl alcohol 1,000 Aldehyde c-12 MNA 1,200 Citronellol ultra 1,500 Cyclacet 1,500 Iso e super 3,500 PTBCH acetate 3,500 Lysmeral base 4,000 Dihydro myrcenol 4,500 Hexyl salicylate 6,000 Galaxolide 10,000 14 10,000 in Hercolyn Dipropylene glycol 44,860 Total 100,000 The two prepared essences were added to a fragrance-free baby liquid detergent at a 1% concentration. The study was conducted on two separate laundry items of the same brand and model. The performance test of the two prepared essences involved two separate laundry items of the same brand and model. Place equal weights of laundry of different textures and materials into the washing machine's drum. The same number of fabrics of each size were included. Baby liquid prepared with both essences 5 60 grams of detergent products were weighed. The weighed essences were added to the washing machine detergent. It was poured into the reservoir. The washing process was carried out at 40°C and 1000 rpm for 60 minutes. The process has been completed. After the process was finished, the fabrics were removed from the machines. The invention concerns a fragrance containing galaxolide and a non-allergenic substitute formulation containing fragrance. Evaluation in terms of sensory and performance tests by perfumers and fragrance analysts. This was done with a panel team of 10 people. After the processes listed above, in galaxolide The fragrance containing a non-allergenic substitute formulation, based on the original fragrance, includes the following: He compared them in this way; - Based on a baby liquid detergent made with a fragrance containing galaxolide, galaxolide 15 Baby liquid made with the same essence but containing a non-allergenic substitute formulation. The detergent was smelled simultaneously from the bottles. The difference between the two products... The comparison was made sensorially. - After washing in the washing machine, the smell of both products will linger while wet. The persistence of the dyes on fabrics was compared sensorially. 20 A panel of 10 people evaluated the essence containing galaxolide using a scoring system between 1 and 5. using the baby liquid detergent in question as a reference, it contains an allergen-free substitute formulation. It was compared with baby liquid detergent that uses fragrance. As stated in the points above. Table 25 shows the evaluation results of the 10-person panel team according to the odor evaluation criteria. This is given in section 7. Both the scent diffusion in liquid detergent and the contact of liquid detergent with fabrics... The persistence of scent on wet and dry fabric after washing is compared sensorially. Individual scores were given. Table 7. Baby Liquid Detergent Scoring System Evaluation Results 30 Essence The smell of the baby in liquid detergent spread The smell of baby liquid detergent for fabrics wet after washing its spread in the fabric and permanence The smell of baby liquid detergent for fabrics dry after washing its spread in the fabric and permanence Scent power Scent power Scent power Containing galaxolide essence 4.3 4.6 3 Non-allergenic substitute formulation containing essence 3.8 3.2 2.1 As can be seen from the table, the use of baby liquid detergent containing 10% galaxolide is recommended. The fragrance developed for this purpose has been prepared in two different ways. The first contains 10% galaxolide, the second... A 10% non-allergenic substitute formulation is available for galaxolide. Both fragrances... The remaining 90% consists of the same raw materials. A comparative analysis of these two essences is as follows: The aim in evaluating this is to find a substitute formulation with the same concentration as galaxolide. It is about how much it affects the smell when used. Scoring is done through a sensory test, that is, by smelling. This has been done. 3 points is the threshold value in the scoring, and therefore the substitution formulation is also the most suitable. It needs to score at least 3 points. Galaxolide is used for the diffusion of scent in baby liquid detergent. The average score given by the panelists to the fragrance containing the substitute formulation was 4.3, while the score for the fragrance containing the substitute formulation was 10. The score was 3.8. Since it received a score above 3, the substitution formulation has the desired properties. It has provided. d. Fragrance created to evaluate scent performance in baby lotion. formulation; 15 To evaluate the fragrance performance of baby liquid lotion containing 30% galaxolide. According to the following fragrance formulation; galaxolide and allergen at a concentration of 30% by weight. Two separate essences containing a substitute formulation, which is not the subject of the invention, have been prepared. The relevant components The contents and percentages are shown in Table 8. 20 Table 8. Baby Lotion Fragrance Components and Proportions. 16 Baby Lotion Fragrance Ingredients By weight % ratio Veloutone 0.020 Anise oil star 0.030 Rose oxide 90-10 HC 0.040 Benzyl salicylate 0.050 Florhydral 0.050 Hexyl acetate 0.050 Liffarome 0.050 Bacdanol 0.060 Coumarin 0.060 Ethyl 2-methyl pentanoate 0.060 Cis-3-hexenyl acetate 0.060 Trivertal PCAS 0.060 Ionone alpha 0.060 Ethyl octanoate 0.060 Melonal 0.070 Nerol Pure 0.070 Allyl capronate 0.100 Calone 1951 10% DPG 0.100 Benzyl acetate 0.100 Cyclamen aldehyde extra 0.100 Ethyl butyrate 0.100 Isoamyl butyrate 0,100 Decalactone delta 0,100 Ethyl hexanoate 0,100 Pyranol 0,100 Phenirat 0,100 Isomenthone %10 DPG 0,100 Cashmeran 0,150 Damascenone total 0,150 Damascone alpha %10 DPG 0,150 Ethyl acetate 0,150 17 Cis-3-hexenol 0,150 Terpineol 0,150 Neryl acetate 0,150 Gamma octalactone 0,150 Allyl cyclohexyl propionate 0,200 Prenyl acetate 0,200 Styrallyl acetate 0,200 Ethyl-2-methyl butyrate 0,250 Frambinone 0,250 Heliotropin %50 DPG 0,250 Vanillin isobutyrate 0,250 Dihydro myrcenol 0,300 Dimethyl benzyl carbinyl acetate 0,300 Cedarwood oil virginia 0,300 Ambrofix 0,350 Hexalon 0,350 Glycolierral 0,350 Aldehyde c-18 (gamma-nonalactone) 0,400 Evernyl 0,400 Cyclaprop 0,400 Dodecalactone delta 0,450 Cıs-3-hexenyl salicylate 0,500 Dimethyl phenyl ethyl carbinol 0,500 Silvial 0,550 Linalyl acetate 0,600 Aldehyde c-14 (gamma undecalactone) 0,650 Citronellyl acetate 0,650 D-limonen 0,750 Geranyl acetate 0,800 Dimethyl benzyl carbinol 1,000 Vanillin 1,000 Maltol %1 DPG 1,000 Canthoxal 1,100 18 Ionone beta 1,200 Geraniol ultra 1,350 Verdox / OTBCH acetate 1,400 Ethyl linalool 1,500 Ethyl vanillin 1,600 Linalool 1,700 Helional 1,800 Lemon oil spain 1,800 Dimethyl benzyl carbinyl butyrate 1,850 Citronellol ultra 2,000 Hexyl salicylate 2,200 Phenyl ethyl alcohol 2,500 Iso e super 3,250 Hexyl cinnamic aldehyde alpha 3,300 Ethyl maltol 3,500 Phenoxanol 3,500 Sandela 85% IPM 6,500 Hedione 11,600 Galaxolide 30,000 Total 100,000 The two prepared essences were added to a fragrance-free baby lotion at a 1% concentration. For the performance test of the two prepared essences; the prepared body lotions were given to 9 participants. The procedure was performed. Participants had the injections applied to specific areas of their bodies (wrists and arms). The strength and longevity of the body lotion's scent over certain time intervals is 5. Evaluations were requested. Evaluations were conducted at the time of application and at 1, 3, 6, 9, and 12 hours. This was done. The results were plotted on a graph and shown in Figure 1. Figure As can be understood from 1; the subject of the invention is a substitute formulation containing an essence that does not contain allergens. The essence containing galaxolide and the other fragrance showed an equivalent change until the 9th hour. Considering the slope lines in the graph, the allergen-free substitute formulation contains fragrance oil 10. and the fragrance containing galaxolide followed an equivalent fragrance intensity and fragrance longevity profile. It can be said. 19 e. Fragrance created to evaluate the scent performance of baby wet wipes. formulation; To evaluate the fragrance performance of baby wipes, a sample containing 6% galaxolide was used. According to the following fragrance formulation; galaxolide and allergen 5 at a concentration of 6% by weight. Two separate essences containing a substitute formulation, which is not the subject of the invention, have been prepared. The relevant components The contents and percentages are shown in Table 9. Table 9. Baby Wipe Fragrance Components and Proportions. Wet Wipe Fragrance Components By weight % ratio Para-cresyl methyl ether 10% DPG 0.010 Allyl heptonoate 10% DPG 0.020 Benzyl salicylate 0.030 Indole 10% DPG 0.030 Anisyl acetate 0.040 Benzyl acetate 0.050 Ethyl acetoacetate 10% DPG 0.050 Eugenol 10% DPG 0.050 Phenyl ethyl formate 10% DPG 0.050 Cyclamen aldehyde 0.060 Phenyl ethyl alcohol 0.060 Allyl amyl glycolate 10% DPG 0.070 Trivertal PCAS 10% DPG 0.100 Vanillin 0.100 Phenirate 0.100 Globanone 0.100 Citronellol ultra 0.150 Coumarin 0.150 Polysantoin 0.150 Isobornyl cyclohexanol 0.150 D-limonene 0.200 Ambrettolide 0.200 Tonalid 0.250 Majantol 0.250 Ethyl vanillin 0.300 Anisaldehyde ex paracresol 0.350 Geraniol ultra 0.500 Mayol 0.550 Musk ketone 0.700 Hexyl cinnamic aldehyde alpha 1,000 Lysmeral base 1,000 Linalool 1,500 Isoraldeine 70 3,500 Florosa 5,000 Ethylene brassylate 6,000 Galaxolide 6,000 Dipropylene glycol 13,180 Iso e super 25,000 Hedione 33,000 Total 100,000 The two prepared essences were mixed into an unscented baby wipe solution and then applied to the wipes. A scented baby wipe was prepared by applying the product at a concentration of 0.2%. For the performance test of the two prepared essences; the prepared wet wipes were given to 10 participants, 5 of each. The procedure was performed and participants had the test applied to specific areas of their bodies (wrists and arms). They evaluate the strength and longevity of the wet wipe's scent over specific time intervals. This was requested. Evaluations were conducted at the time of application and at 1, 3, 6, and 9 hours. The results were plotted on a graph and shown in Figure 2. As can be seen from Figure 2... Accordingly, the allergen-free substitute formulation contains fragrance that retains scent for up to 2 hours. Although its concentration remains lower than that of the galaxolide-containing essence. 2. After an hour, a slightly weaker scent, in a direction parallel to the galaxolide-containing fragrance. It has continued with its intensity. When the slope lines in the graph in question are evaluated, it is seen that... The fragrance containing galaxolide lasts 19 hours, while the fragrance containing the allergen-free substitute formulation lasts 16 hours. It can be said that the fragrance lasts for approximately 15 hours. Allergen-free. 21 The fragrance in this formulation has a slightly lower scent intensity compared to the fragrance containing galaxolide. Although it describes a similar profile, it can be said that its values ​​are close to those of an essence containing galaxolide. f. Fragrance formulations created to evaluate the fragrance performance in perfume; To evaluate the fragrance performance of perfumes containing 60% galaxolide, the following were used: According to the fragrance formulation; galaxolide at a concentration of 60% by weight and non-allergenic. The subject of the invention is the preparation of two separate essences containing a substitute formulation. The relevant component contents and The rates are shown in Table 10. Table 10. Perfume Essence Components and Proportions. Perfume Essence Components By weight % ratio Dynascone 10% 0.010 Eugenol 0,010 Cis-3-hexenol 0,010 Cis-3-hexenyl acetate 0,010 Isobornyl acetate 0,010 Camphor 0,010 Methyl octine carbonate 0,010 Trivertal PCAS 0,010 Estragole 0,010 Litsea cubeba oil 0,010 Oakmoss %50 DPG 0,010 Trans-2-cis-6-nonadienol %1 DPG 0,010 Allyl cyclohexyl propionate %10 DPG 0,020 Citronellol ultra 0,020 Melonal 0,020 Rose oxide %10 DPG 0,020 Undecavertol %10 DPG 0,020 Canthoxal 0,020 Armoise oil 0,030 Geraniol ultra 0,040 22 Pine oil Siberia 0,040 Cypriol oil MD 0,040 Coranol 0,040 Davana oil 0,040 Labdanum abs light %10 DPG 0,040 Oud synth 0,040 Lavandin oil Abrialis 0,040 Pink pepper CO2 extract 0,040 Ethylene brassylate 0,050 Cassis base 0,050 Elemi oil Philippines 0,050 Calone 1951 0,060 Evernyl 0,060 Methyl pamplemousse 0,070 Aldehyde c-14 ( gamma undecalactone) 0,080 Rosalin 0,080 Geranyl acetate 0,080 Phenirat 0,080 Helvetolide 0,080 Jasmıne absolute Morocco 0,080 Cedarwood white key 0,080 Vanilla extract %10 DPG 0,080 Dihydro myrcenol 0,150 Helional 0,150 Habanolide 0,200 Ambrox DL 0,200 Linalool 0,300 Lyrasub 0,300 Cashmeran 0,350 Linalyl acetate 0,350 Coumarin 0,400 Ethyl linalool 0,400 Patchoulı oil light Sulawesi 0,400 23 Rose absolute 0,400 Ambrofix 0,400 Vertofix coeur 0,450 Bergamot oil furocoumarine free 0,600 Lysmeral base 0.650 Hexyl cinnamic aldehyde alpha 0,800 Benzyl acetate 0.800 Muscenone 0.800 Methyl ionone gamma coeur 0.800 Benzyl salicylate 0.850 Cedryl methyl ether 1,100 Phenyl ethyl alcohol 1,600 PTBCH acetate 1,600 Amber Xtreme 10% DPG 2,100 Hedione 2,200 Iso e super 2,400 Exaltolide total 4,000 Tonalid 5,000 Dipropylene glycol 8,640 Galaxolide 60,000 Total 100,000 The two prepared essences were added to a standard eau de toilette (EDT) formulation containing 20% ​​ethanol. It was applied at the specified concentration. For the performance test of the two prepared essences; the prepared The perfumes were applied to 7 participants. The participants applied them to specific areas of their bodies. (wrists and arms) the strength of the perfume's scent at certain time intervals and 5 They were asked to evaluate its persistence. Evaluations were made at the time of application and at the 1st, 3rd, 6th, 9th, and 12th weeks. These measurements were taken over several hours. The results were plotted on a graph and shown in Figure 3. This is shown. As can be seen from Figure 3, the subject of the invention is an allergen-free substitute. The formulation containing fragrance has a higher scent intensity than the galaxolide-containing fragrance for up to 6 hours. It continued at lower values ​​than before. After 6 hours, allergen-free substitute 10 The formulation containing the fragrance continues with equivalent values ​​to the fragrance containing galaxolide, up to 10. The scent intensity remained at higher levels even after an hour. In terms of scent longevity... 24 The fragrance containing an allergen-free formulation and the fragrance containing galaxolide follow a similar path. It can be said. g. Galaxolide compound and its non-allergenic substitute, which is the subject of the invention. Comparative evaluation of physical analyses of the formulation 5 Baby shampoo containing galaxolide and a non-allergen substitute formulation, baby concentrate. fabric softener, baby lotion, baby wipes, baby liquid detergent, and perfume. The concentrations of the essences in the categories were measured at 20°C. The relevant results are in 2 tables. This is given in Tables 11 and 12. 10 Table 11. For baby shampoo, baby concentrated fabric softener, and baby liquid detergent. density results Essence Baby shampoo used for the test essence Baby concentrate for fabric softener test the essence used Baby liquid detergent test essence used for Density (g / cm3) Density (g / cm3) Density (g / cm3) Containing galaxolide essence 0.9853 1.0618 0.9845 Non-allergenic substitute formulation containing essence 0.9911 1.0608 0.9829 Table 12. Density results for baby lotion, baby wipes and perfume. 15 Essence Baby lotion test essence used for Baby wipe test essence used for For perfume testing the essence used Density (g / cm3) Density (g / cm3) Density (g / cm3) Containing galaxolide essence 0.9884 0.9845 0.9953 Non-allergenic substitute formulation containing essence 0.9944 0.9829 1.0081 As can be seen from the tables above; the allergen developed as an alternative to galaxolide The concentration of the fragrance is increased by using a non-substitute formulation in the composition of the relevant essences. It does not cause a significant change in their values. The most important of the essences in 6 different categories. It is a perfume essence containing a high concentration of galaxolide, and the galaxolide concentration It is 60%. This essence contains 60% galaxolide replaced by 60% non-allergenic substitute formulation 5. Even when used, it increases the concentration of the essence by 1.28%. (1.0081– (0.9953) / 0.9953*100 = 1.28%, standard deviation is considered normal.) Baby shampoo containing galaxolide and a non-allergenic substitute formulation, baby concentrate. fabric softener, baby lotion, baby wipes, baby liquid detergent and perfume 10 The refractive indices of the essences in the categories were measured at 20°C. The relevant results are in 2 tables. These are given in Tables 13 and 14. Table 13. Breakdown of contents for baby shampoo, baby concentrated fabric softener, and baby liquid detergent. index results 15 Essence Baby shampoo for the test the essence used Baby concentrate for fabric softener test the essence used Baby liquid detergent test essence used for Refractive index Refractive index Refractive index Containing galaxolide essence 1.4757 1.4993 1.4713 Non-allergenic substitute formulation containing essence 1.4556 1.5002 1.4757 Table 14. Refractive index results for baby lotion, baby wipes, and perfume. Essence Baby lotion for the test the essence used Baby wipe test essence used for Used for perfume testing. essence Refractive index Refractive index Refractive index Containing galaxolide essence 1.5042 1.4713 1.5124 Non-allergenic 1.4795 1.4757 1.4625 26 substitute formulation containing essence As can be seen from the tables above; the allergen developed as an alternative to galaxolide Breakdown of the essence by using a non-substitute formulation in the composition of the relevant essences It does not cause a significant change in index values. 5 Baby shampoo containing galaxolide and a non-allergenic substitute formulation, baby concentrate. fabric softener, baby lotion, baby wipes, baby liquid detergent, and perfume. The flash points of the fragrances in these categories were measured. The relevant results are presented in 2 tables. This is given in Tables 15 and 16. 10 Table 15. Shine rating for baby shampoo, baby concentrated fabric softener, and baby liquid detergent. point temperature results Essence Baby shampoo used for the test essence Baby concentrate for fabric softener test the essence used Baby liquid detergent test essence used for Flash point (°C) Flash point (°C) Flash point (°C) Containing galaxolide essence 89 107 116 Non-allergenic substitute formulation containing essence 89 107 116 Table 16. Flash point temperature for baby lotion, baby wipes, and perfume. results Essence Baby lotion test essence used for Baby wipes used for the test essence For perfume testing the essence used Flash point (°C) Flash point (°C) Flash point (°C) 97 116 117 containing galaxolide 27 essence Non-allergenic substitute formulation containing essence 93 116 114 As can be seen from the tables above; the allergen developed as an alternative to galaxolide The use of a non-substitute formulation in the composition of the relevant essences enhances the fragrance's brilliance. This does not cause a significant change in the point values. 5 The above regulations apply only to the technical concept and features of the present invention. The aim is to explain and the purpose of the present invention is to enable experts in the field to understand the present invention. the aim is to enable him to understand the content and apply the existing invention, and the scope of the existing invention. This is not limited to this. Equivalent modifications or modifications made in accordance with the spirit of the invention. The aim is to include the modifications within the scope of the invention. Industrial Application of the Invention The invention is intended for use particularly in the cosmetics and fragrance industries; it has been patented by the European Union for 15 years. With the regulation numbered 2023 / 1545 regarding fragrance allergens, new allergen fragrances have been introduced. 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta- which was added as a raw material an allergen-free alternative developed as an alternative to the compound gamma-2-benzopyran (galaxolide) It relates to fragrance formulation and is suitable for industrial application. The invention is not limited to the above example applications and can be easily developed by a person skilled in the field. It can reveal other different applications of the invention. These are the claims and demands of the invention. It should be considered within the scope of protection. 28 References [1] https: / / eur-lex.europa.eu / eli / reg / 2023 / 1545 / oj Page 14 No 336

Claims

29 REQUESTS 1. New in the European Union regulation 2023 / 1545 on fragrance allergens. 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8- added as allergen fragrance raw material As an alternative to hexamethylcyclopenta-gamma-2-benzopyran (galaxolide) compound 5 to be developed; - 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H-inden-4-one (cashmeran), - cyclohexadec-8-en-1-one (globanone), - 1-propanol, 2-(1-(3,3-dimethylcyclohexyl)ethoxy)-2-methyl-,1-propanoate (helvetolide), - dipropylene glycol, 10 - acetic acid, (1-oxopropoxy)-1-(3,3-dimethylcyclohexyl) ethyl ester (romandolide), - oxacycloheptadec-10-en-2-one (ambrettolide), - 1,4-dioxacycloheptadecane-5,17-dione (ethylene brassylate) A formulation characterized by containing 15.

2. 1,2,3,5,6,7-hexahydro-1,1,2,3,3-pentamethyl-4H-inden- in the range of 0.1-1.5% by weight. 4-on (cashmeran), cyclohexadec-8-en-1-on in concentrations ranging from 0.1% to 1.5% by weight. (globanone), 0.1-1.5% by weight of 1-propanol, 2-(1-(3,3- (dimethylcyclohexyl)ethoxy)-2-methyl-,1-propanoate (helvetolide), 50-90% by weight 20 dipropylene glycol in the range of 0.5-3% by weight, acetic acid, (1-oxopropoxy)- 1-(3,3-dimethylcyclohexyl) ethyl ester (romandolide), in the range of 0.5-3% by weight. oxacycloheptadec-10-en-2-on (ambrettolide) and 1,4- in the range of 15-35% by weight. Characterized by containing dioxacycloheptadecane-5,17-dione (ethylene brassylate) Formulation according to Claim 1. 25 3. Use of the formulation in the fragrance and cosmetics field according to either of claims 1-2.

4. The formulation is used in infant care products according to either claim 1-2; infant detergent, baby fabric softener, baby wipes, baby shampoo, baby soap, baby 30 The lotion is allergen-free and suitable for personal or non-personal use, and is ideal for people with sensitive skin types. Cleaning and care products, toddler care products and wipes, wet or dry. allergen-free cosmetics including wipes, detergents, fabric softener, shampoo and soap. Its use in the field of external cleaning and maintenance. 35

Images