FOOD SUPPLEMENT AND PRODUCTION METHOD EFFECTIVE ON COGNITIVE FUNCTIONS

Abstract

The invention specifically relates to a food supplement, derived from black carrot and chicory roots, that prevents and / or alleviates the effects of cognitive impairment in Alzheimer's-like dementia, prevents toxic effects by being obtained from natural materials, and reduces the risk of developing and / or progressing neurodegenerative diseases through its antioxidant effect, as well as the food supplement production method used to obtain this food supplement.

Claims

1. By affecting the central nervous system, it can prevent and / or mitigate the effects of cognitive impairment. elimination, enhancement of antioxidant, anti-inflammatory and prebiotic effects, and toxic It is a food supplement that helps reduce the effects, and its characteristic is; - Anthocyanin nanoparticles encapsulated with chitosan and derived from black carrots; - Inulin encapsulated with chitosan and obtained from chicory root; It includes.

2. This is a food supplement that complies with Claim 1, and its characteristic feature is achieving the highest possible neuroprotective effect. The recommended dosage of anthocyanin nanoparticles is 200-400 mg per dose.

3. This is a food supplement that complies with Claim 1, and its characteristic feature is that it is formulated to achieve the highest probiotic effect. The amount of inulin should be 40-60g per dose.

4. This is a food supplement that complies with Claim 1, and its characteristic feature is that it is used to achieve the highest possible effect. It contains 400-600mg of vitamin C per dose.

5. By affecting the central nervous system, it can prevent and / or mitigate the effects of cognitive impairment. elimination, enhancement of antioxidant, anti-inflammatory and prebiotic effects, and toxic a food supplement that enables the production of a food supplement that helps reduce the effects It is a production method, and its characteristic is; - dissolving chitosan in a 2% by weight glacial acetic acid solution and homogenizing the solution by stirring with a magnetic stirrer and obtaining a chitosan solution with a pH value in the range of 4.5-5; - Extraction of anthocyanin from black carrots and conversion of the resulting anthocyanin into chitosan by adding it to the solution and mixing it in a homogenizer to obtain a homogeneous distribution. being done; - a 2% by weight sodium citrate solution to the homogeneously distributed mixture chitosan by adding and mixing the mixture using a mechanical mixer Precipitation of nanoparticles; 14 - after sedimentation, the mixture is centrifuged using a refrigerated centrifuge device. Separation of nanoparticles from the supernatant by means of; - after the separated nanoparticles are washed with distilled water at least 3 times Freezing the nanoparticle suspension by placing it in a deep freezer; - Lyophilization of the frozen suspension using a lyophilizer; - after soaking chicory roots in distilled water for inulin extraction Washing by spinning using a heated mixer; - removing the water from the washed roots using a vacuum evaporator and carbon purification by filtration; - extraction of inulin by lyophilizing the purified roots. completion; - Chitosan in 1% by weight acetic acid solution for inulin encapsulation. resolution; - homogenizing the solution by stirring in a magnetic stirrer and adjusting the pH setting the value to the range of 4.5-5.5; - by mixing the resulting inulin and chitosan solution using a homogenizer achieving a homogeneous distribution; - 0.1-0.5% w / v sodium tripolyphosphate (TPP) solution added to a homogeneous mixture of inulin and chitosan. Observation of particle formation by adding particles; - the mixture is spun using a refrigerated centrifuge and the particles are separated. separation; - Separating the supernatant from the mixture and washing it with distilled water at least 4 times to remove it. removal of inulin; - Inulin is obtained by freezing the washed supernatant and recovering it using a lyophilizer. completion of encapsulation; - by adding chitosan-encapsulated inulin to the obtained chitosan-encapsulated anthocyanins obtaining the aforementioned food supplement It includes the steps. 15 6. A food supplement manufacturing method that complies with claim 5, characterized by the dissolution of chitosan. This then involves stirring the mixture in a magnetic stirrer for 14 hours.

7. A food supplement manufacturing method that complies with Claim 5, characterized by the conversion of anthocyanin to chitosan. After adding it to the solution, it is homogenized at 18000rpm for 6-10 minutes. It involves the mixing step.

8. A food supplement manufacturing method in accordance with Claim 5, characterized by the aforementioned sodium citrate. The solution should be in an amount of 100ml.

9. A food supplement production method that complies with Claim 5, characterized by the use of chitosan nanoparticles. This involves mixing the mixture in a mechanical mixer for 1 hour to allow it to precipitate.

10. A food supplement manufacturing method that complies with Claim 5, characterized by containing nanoparticles. Analysis of the supernatant for free drug content after separation of the supernatant. It includes the step of being done.

11. A food supplement production method that complies with Claim 5, characterized by containing nanoparticles. Centrifugation at 11000 rpm for 8-10 minutes to separate the supernatant. It includes the step.

12. A food supplement production method in accordance with Claim 5, characterized by; nanoparticle suspension (-) Freezing step by keeping it in a deep freezer at a temperature of 70-90 °C for 24 hours. It includes.

13. A food supplement manufacturing method in accordance with Claim 5, characterized by the production of a frozen suspension. It involves a lyophilization step at a temperature of 10-30 °C for 24 hours.

14. A food supplement production method in accordance with claim 5, characterized by the processing of chicory roots at 85-95 °C. After being left at the temperature for 5 minutes, it is stirred for 2 hours using a heated mixer. It involves washing the animal by turning it over for a certain period of time.

15. A food supplement production method in accordance with claim 5, characterized by the roots being at (-) 70-90 °C It involves a lyophilization step at a temperature of 35°C for 12 hours.

16. A food supplement manufacturing method in accordance with Claim 5, characterized by the conversion of chitosan into acetic acid. After being dissolved in the solution, it is stirred for 12 minutes on a magnetic stirrer. This involves the step of homogenizing the solution. 16 17. A food supplement manufacturing method in accordance with Claim 5, characterized by the solution of inulin and chitosan. The mixture is homogenized by mixing for 10 minutes at a speed of 10000-15000 rpm using a homogenizer. This includes the step of obtaining the distribution.

18. A food supplement production method in accordance with Claim 5, characterized by the production of homogeneous inulin and chitosan. The mixture was centrifuged in a refrigerated centrifuge for 30 minutes at 15,000 rpm. It involves the step of separating the particles by rotating them.

19. A food supplement manufacturing method in accordance with Claim 5, characterized by the washing of inulin and chitosan. This involves freezing the supernatant at a temperature of (-) 70-90 °C.

20. A food supplement production method in accordance with Claim 5, characterized by the encapsulation of the resulting chitosan. The aforementioned food supplement is formulated by adding inulin and vitamin C to anthocyanins and chitosan encapsulation. It includes the step of obtaining it. 1 Specifications FOOD SUPPLEMENT AND PRODUCTION METHOD EFFECTIVE ON COGNITIVE FUNCTIONS Technical Area The invention aims to prevent the negative effects of cognitive impairments by affecting the central nervous system. and / or is related to a food supplement and production method that enables its removal. The invention, in particular, uses black carrot and chicory roots to treat Alzheimer's-like types of dementia. Preventing and / or alleviating the effects of cognitive impairment that may arise, using natural materials. by obtaining it, toxic effects can be prevented and neurodegenerative diseases can be treated with its antioxidant effect. a food supplement that helps reduce the risk of occurrence and / or progression of this condition, and the benefits of this food supplement. It relates to the food supplement production method that enables its obtainment. State of the Art Cognitive impairments are generally characterized by cognitive impairments that have known or suspected biological causes. mental disorders that negatively affect their abilities and daily functioning It is characterized as occurring at the individual's level of functionality prior to the onset of the disease. Disorders that manifest with significant changes usually affect the patient's thinking, learning, changes in mental functions such as memory, attention, decision-making, problem-solving, and perception These include disorders, mostly related to neurological, psychiatric, or metabolic diseases. These disorders, which can manifest as various physical or mental conditions or brain disorders, factors affecting functioning such as substance use and / or withdrawal, and the patient's advancing age. It also manifests itself through various factors, affecting daily life and limiting the individual's independence. The negative effects can increase rapidly. Our ability to perform cognitive functions; It depends on brain functions such as thinking, reasoning, storing and recalling information, especially in progressive forms of deterioration such as Alzheimer's disease, there is a noticeable difference in these processes. Deterioration occurs, and the loss of function progresses gradually. In general, the onset of the disease... The more widespread the damage becomes after the onset of epistaxis, the greater the dysfunction. These disorders, defined as neurodegenerative diseases, cause brain cells to lose their function over time. loss and / or dysfunction of cognitive abilities, movement These disorders manifest themselves with various symptoms such as balance problems and speech difficulties. The brain... Neurodegenerative diseases, which affect different regions and lead to various symptoms, include 2 Alzheimer's disease, Parkinson's disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's disease, Multiple sclerosis (MS) and dementia are among the diseases included. Especially Alzheimer's and Lewy body syndrome. Early diagnosis of cognitive impairments that can occur in diseases such as dementia and Parkinson's disease is crucial for the disease. This is extremely important in controlling its progression. Oxidative stress, inflammation, and mitochondrial dysfunction are part of the pathophysiology of these diseases. It contributes significantly. The accumulation of all these related events induces apoptosis. It is thought to be responsible for dopaminergic neurodegeneration. In this context, the aforementioned The biggest problems in administering drugs used to treat diseases are the drug's active ingredients. The inability of these substances to cross the blood-brain barrier, low bioavailability, and toxicity. This presents a problem. Increasing the effectiveness of the drugs used and their transport to the brain... Various nanoformulations have been developed to facilitate this. However, traditional chemical Synthesis methods involve harsh reaction conditions, the use of toxic chemicals, and environmental negative impacts. Due to the effects, there are concerns regarding its use. Therefore, a more environmentally friendly and Developing sustainable synthesis methods is necessary, and this is a critical issue for researchers. It is becoming a target. Plant extracts can be used to reduce or prevent the aforementioned negative effects. Biosynthesis of nanoparticles is preferred. The phytochemicals used in these applications, As natural compounds, they possess strong antioxidant and anti-inflammatory properties and have neuroprotective effects. It offers benefits. However, the low bioavailability of these components hinders their therapeutic use. This limits their potential, and therefore, increasing their ability to cross the blood-brain barrier and Therefore, their bioactivity needs to be improved. Furthermore, bioavailability should be increased. The problem of toxicity must also be minimized. In addition to all this, the gut and... Because the relationship between the brain and phytochemicals has been revealed by many different studies, the cognitive aspect of phytochemicals... to support its effect on functions, a support for the gut microbiota The need for its use together with the component arises. "Blueberry Supplementation in Neuronal Health and" is included in the state of the art. In the article titled “Protective Technologies for Efficient Delivery of Blueberry Anthocyanins”, wild Mersin helps minimize the effects of age-related diseases and improves learning and memory in children. Its use as a daily edible supplement source to improve development is described. Wild Myrtle is consumed as a healthy fruit that provides various benefits to the nervous system. It is explained and the most frequently mentioned compounds among the components in blueberries are: It is stated that there are 35 anthocyanins. In the review in the article, neuroprotection and In terms of positive outcomes regarding intervention in neurodegenerative conditions, animal and human 3 including studies ranging from in vitro cell studies to in vivo studies of wild animals The focus is on neurological research of blueberries. Additionally, the benefits of blueberries in daily life are being explored. Understanding the bioavailability of anthocyanins and the factors affecting their consumption is crucial for making informed decisions about their consumption. Information about important factors is provided. In this context, blueberries or bilberries the different forms of supplementation and which of these forms maximizes the benefits of blueberries It is considered appropriate to elevate it to the highest level. Within the document in question, Using black carrot and chicory roots, treatments are applied to treat Alzheimer's-like types of dementia. Prevention and / or mitigation of cognitive impairment effects, while preventing toxic effects. A food aimed at reducing the risk of developing and / or progressing neurodegenerative diseases. The supplementation and production method are not mentioned. The patent document numbered CN116035209A, relating to the known technique, is based on microfluidic technology. A chitosan-modified anthocyanin nanoliposome and its application are described. Nano liposomes with high encapsulation efficiency for anthocyanin wrapping, microfluidic multi-encapsulation. the ratio of phase channel contents, buffer solution condition, flow rate, and other coefficients Particle size and encapsulation efficiency are improved through optimized control of response values. It is prepared by taking it as a systematic evaluation. Chitosan modification, nano the storage time, temperature resistance, stability of liposomes under a gastrointestinal system and It improves its functionality. Thus, anthocyanin is a functional food due to its environmental sensitivity. The difficulty of obtaining and using it in this way by the human body is overcome. The document in question describes how to use black carrots and chicory roots to induce Alzheimer's-like conditions. Prevention and / or alleviation of cognitive impairment effects occurring in types of dementia, toxic reducing the risk of developing and / or progressing neurodegenerative diseases while preventing their effects There is no mention of a food supplement or a production method related to it. In conclusion, Alzheimer's-like dementia can be treated, particularly using black carrots and chicory roots. Prevention and / or alleviation of cognitive impairment effects occurring in certain types of diseases, antioxidant by supporting the gut microbiota, it can prevent the development and / or spread of neurodegenerative diseases. a food that reduces the risk of progression by increasing bioavailability while reducing toxic effects supplementation and the food supplement production method that enables the production of this food supplement It needs to be improved. 35 4 Purpose of the Invention The present invention meets the aforementioned requirements and eliminates potential disadvantages. a food supplement and food supplement production method that removes and provides some additional advantages It is related. The main purpose of the food supplement and production method described in this invention is to affect the central nervous system. to prevent and / or alleviate the effects of cognitive impairment and thus neurodegenerative a food supplement and product that helps reduce the risk of developing and / or progressing diseases The method is to obtain it. Another aim of the invention is to achieve neuroprotective benefits by increasing its antioxidant and anti-inflammatory activity. The goal is to obtain an effective food supplement and production method that enables this. Another aim of the invention is to utilize natural components in harsh reaction conditions and to minimize toxicity. by reducing the use of chemicals and their negative environmental impacts, thus improving the patient's health. A functional food supplement and production method that reduces toxic effects has been obtained. It is done. Another aim of the invention is to increase bioavailability and therapeutic effects by crossing the blood-brain barrier. The goal is to obtain an effective food supplement and production method that enhances their potential. Another aim of the invention is to act as a prebiotic and positively influence gut microorganisms. by regulating / supporting the gut microbiota and cognitive functions an efficient food supplement and production method that enhances its effect It is done. Another aim of the invention is to preserve the effectiveness of the natural components in its composition and to ensure their usability. The goal is to obtain a food supplement and a production method that allows for an increase in shelf life. Another aim of the invention is to provide ease of use for the user and to extend shelf life. It is the process of obtaining food supplements and their production method. To achieve the above objectives in their most general form, the central nervous system, developed with the present invention, must be used. Food that affects the 35 system and helps prevent and / or alleviate the effects of cognitive impairment. The supplement consists of anthocyanin nanoparticles encapsulated with chitosan and derived from black carrots; It contains inulin, derived from chicory root, encapsulated in chitosan. The food supplement production method developed with the current invention involves dissolving chitosan and the solution... homogenization and production of chitosan solution; extraction of anthocyanin from black carrots. and obtaining a homogeneous distribution; the homogeneous mixture into the sodium citrate solution Precipitation of chitosan nanoparticles by addition and mixing; centrifugation of the mixture Separation of nanoparticles by processing; nanoparticles after washing Freezing the suspension in a deep freezer; lyophilizing the frozen suspension; Washing chicory roots by spinning them using a heated mixer; removing water from the roots removal and purification by carbon filtration; inulin by lyophilization of roots. Completion of extraction; dissolution of chitosan in acetic acid solution; mixing. homogenization of the solution; homogeneous distribution by mixing the inulin and chitosan solution. The formation of particles is achieved by adding a sodium tripolyphosphate (TPP) solution to the mixture. observation; rotation of the mixture and separation of particles; removal of supernatant from the mixture Separation and washing to remove free inulin; freezing of the washed supernatant and Completion of inulin encapsulation upon release; chitosan encapsulates anthocyanins, This includes the steps for obtaining the food supplement by adding inulin to chitosan encapsulation. The structural and characteristic features and all the advantages of the invention are given in the figures below. The detailed explanation, written with references to figures, makes it clearer. This will be understood, and therefore the evaluation should also take these figures and detailed explanations into account. It must be done by taking it. Detailed Description of the Invention This detailed explanation describes the food supplement and the preferred method of producing the food supplement that is the subject of the invention. The structures discussed are explained solely for the purpose of facilitating a better understanding of the subject. The current invention enhances the effects of cognitive impairment by acting on the central nervous system. prevention and / or elimination of antioxidant, anti-inflammatory and prebiotic effects A food supplement encapsulated with chitosan that enhances and reduces toxic effects. and derived from black carrots, preferably used to achieve the highest neuroprotective effect. 200-400 mg anthocyanin nanoparticles per dose; encapsulated with chitosan and chicory. Obtained from 35 roots, preferably per dose to achieve the highest probiotic effect. 40-60g inulin; preferably 400-600mg C per dose for maximum effect. It contains vitamin 6. In a sample application of the food supplement developed with the present invention, a phytochemical is used. Anthocyanins have neuroprotective effects in patients thanks to their strong antioxidant and anti-inflammatory effects. It demonstrates and, thanks to its chitosan-encapsulated structure, crosses the blood-brain barrier, increasing bioavailability. This increases the efficiency of brain cells, thereby reducing the risk of neurodegenerative diseases. It supports short-term and long-term memory by enhancing communication between them. Chitosan-coated inulin. by supporting the gut microbiota, it contributes to the bioavailability of anthocyanin. and the combined action of these two components enhances the effect on the central nervous system. It demonstrates a strong therapeutic effect in preventing and / or alleviating the effects of cognitive impairment. It is also possible to avoid problems during or after use because both components are natural materials. This prevents toxic effects from occurring in the patient. In this context, anthocyanins are naturally occurring antioxidants that are effective against neurodegenerative diseases. It is well known for its preventive effects in disorders. Black carrots [Daucus carota L. ssp. sativus] [Aleph. var. atrorubens] is a rich source of anthocyanins, mostly acylated cyanidins. They contain derivatives based on certain compounds, which makes them more stable. However, black carrots... In terms of the potential neuroprotective effect of anthocyanins (BCAs), particularly high by increasing metabolic activity and reducing membrane damage at concentrations (50, 100 lg / ml) It exhibits cytoprotective effects. BCA scavenges MPP+-derived reactive oxygen species (ROS). It exhibits antioxidant activity by protecting dopaminergic neurons from ROS-mediated apoptosis. This is due to BCA's high antioxidant and antiapoptotic activity and lack of cytotoxicity. This indicates that it has a neuroprotective effect. Consequently, BCA is high. With its stability and potential neuroprotective effects, it functions as a neuroprotective agent. It shows. Antioxidant intake (vitamins C and E, flavonoids, carotenoids) and cognitive function or The relationship between vitamin C deficiency and the risk of dementia is discussed. Vitamin C deficiency leads to metabolic disorders. This is because it leads to symptoms of folic acid deficiency. Another important example of this is diet. Iron absorption from its sources is dependent on ascorbic acid. Also, high levels of vitamin C... Studies have investigated oxidative stress markers, mitochondrial breakdown, and cortical peri-infarction. It reduces apoptosis in neurons [Morris-Blanco KC, Chokkalla AK, Kim T, Bhatula S, Bertogliat] MJ, Gaillard AB, Vemuganti R. High-Dose Vitamin C Prevents Secondary Brain Damage After Stroke via Epigenetic Reprogramming of Neuroprotective Genes. Transl Stroke Pic. 2022 Dec;13(6):1017-1036. doi: 10.1007 / s12975-022-01007-6. Epub 2022 Mar 20. PMID: 35306630; 35 PMCID: PMC9485293. Ramakrishna T. Vitamins and brain development. Physiol Res. 1999;48(3):175-87. PMID: 10523053]. 7 The current invention enhances the effects of cognitive impairment by acting on the central nervous system. prevention and / or elimination of antioxidant, anti-inflammatory and prebiotic effects obtaining a food supplement that enables the enhancement and reduction of toxic effects. The food supplement production method involves dissolving chitosan in a 2% by weight glacial acetic acid solution. the solution is dissolved and preferably stirred using a magnetic stirrer for 14 hours. homogenization and protonation, viscosity increase, loss of homogenization and To prevent the risk of precipitation and achieve optimum solubility, the pH value should be in the range of 4.5-5. Obtaining chitosan solution; obtaining anthocyanin from black carrot and the resulting... anthocyanin is added to the chitosan solution and homogenized in a homogenizer, preferably for 6-10 minutes. Mixing at 18000 rpm to achieve homogeneous distribution; the homogeneously distributed mixture... preferably added to a 100ml sodium citrate solution at a concentration of 2% by weight, and Chitosan is obtained by mixing the mixture, preferably for 1 hour, using a mechanical mixer. Precipitation of nanoparticles; centrifugation of the mixture in a refrigerated centrifuge after precipitation. nanoparticles are produced by centrifuging them, preferably at 11000 rpm for 8-10 minutes. Separation from the supernatant and preferably analysis of the supernatant for free drug content. This involves the process of precipitation, which facilitates nanoparticle formation, while centrifugation... Nanoparticles are separated from the supernatant and through a combination of centrifugation processes, Homogeneous, pure chitosan nanoparticles of the desired size are obtained. Subsequently, After the separated nanoparticles are washed at least 3 times with distilled water, the nanoparticles The suspension should preferably be stored in a deep freezer at a temperature of (-) 70-90 °C, preferably for 24 hours. Freezing by holding it in place; freezing the frozen suspension using a lyophilizer, preferably for 10-30 days. Lyophilization of chicory roots for inulin extraction at °C temperature for 24 hours; preferably after being left in distilled water at a temperature of 85-95 °C for 5 minutes, heated and stirred. Washing the roots by rotating them, preferably for 2 hours; vacuuming the water from the washed roots. Removal using evaporation and purification by carbon filtration; purification The processed roots are preferably lyophilized at a temperature of (-) 70-90 °C for 12 hours. Completion of inulin extraction; 1% by weight of chitosan for inulin encapsulation. Dissolving in acetic acid solution at a certain ratio; preferably stirring for 12 minutes on a magnetic stirrer. The solution is mixed until homogenized, and the pH value is adjusted to the range of 4.5-5.5; The resulting inulin and chitosan solution is mixed and homogenized using a homogenizer, preferably at 10°C. Mixing for 1 minute at 10000-15000 rpm to obtain a homogeneous distribution; homogeneous A 0.1-0.5% w / v sodium tripolyphosphate (TPP) solution is added to a mixture of inulin and chitosan to produce particles. Observation of formation; centrifugation of the mixture using a refrigerated centrifuge, preferably for 30 minutes. Spinning at 15,000 rpm for 35 minutes to separate the particles; extracting the supernatant from the mixture. Separation and removal of free inulin by washing with distilled water at least 4 times; preferably (-) 70-8 Inulin encapsulation by freezing at 90 °C and recovering using a lyophilizer. Completion of the process; resulting in chitosan-encapsulated anthocyanins, chitosan-encapsulated inulin and This involves the steps to obtain the aforementioned food supplement, preferably with the addition of vitamin C. In a sample analysis, the use of the developed food supplement by patients diagnosed with dementia was examined. EEG recordings were taken twice, once before and once after. The EEG recordings contain 10-20 locations. It is monopolar in its system. The reference electrode is the M electrode, which is placed on the mandibular bone. Electrodes were used and recordings were taken with eyes closed in a relaxed state. The recordings With the AntNeuro eego sports model EEG device, patients can have EEGs performed in suitable environments within their own buildings. Noise analysis was performed before and after recording, due to environmental changes. Potential electrical noise has been controlled. The system is not connected to the mains power supply. Its ability to function significantly reduces mains noise (50 Hz). In addition to these, EEG After the recordings are made, noise removal is performed using the Independent Component Analysis (ICA) method. The analysis was carried out mostly in Matlab and eeglab. Python programming was also used. The method for comparison is Spectral Power Density (PSD) analysis and the frequency obtained from it. It is a spatial map of the bands on the head. In addition, it examines the increase in irregularity. Quadratic Sample Entropy (QSE), a measure of entropy, was applied. Cognitive impairment In patients exhibiting this behavior, complexity and irregularity (signal irregularity that is not physiological) are reduced. This is reported in the literature. The pwelch method was used in the implementation of PSD. After noise reduction, the entire signal... The data was processed and the average value was used for each electrode. In the applied method, temporal No analysis has been performed. Individual differences and PSDs of separate electrodes for each individual. After calculation, the total average for overall comparison is calculated both on an individual and electrode basis. The following graph was obtained by taking these measurements (Graph 1). 9 Group Comparison Figure 1. Spectral Power Density (PSD) analysis graph. In Figure 1, the "first" line shows the pre-use state, and the "second" line shows the post-use state. Looking at the overall average obtained here, delta (1-4 Hz), beta (2-30 Hz) and low gamma Changes are observed in the (30-45 Hz) bands. Statistical analysis performed for PSD analysis (t- The test results showed a significant change in the beta3 (25-30 Hz) and gamma (30-45 Hz) bands. It has been observed that, in this context, the general average gives us a power in these frequency bands after use. This summarizes showing that there is an increase. It shows where this difference lies topologically. Spatial analysis was performed for this purpose. Topological head maps are given below (Graph 2). Figure 2. Graph of topological head maps. In Figure 2, respectively, Beta1 (12-15 Hz), Beta2 (15-22 Hz), Beta3 (22-30 Hz) and Low Gamma (30- Electrode-based topological density maps for frequency bands (45 Hz) are presented. According to the data, a power increase is observed concentrated in electrodes F7, F3, T5, and C3. Figure 3. QSE analysis graph. Additionally, the QSE analysis performed in Figure 3 showed significant differences in the delta and gamma frequency bands. Changes have been observed. The graph shows how the QSE values ​​changed before and after the event. The color map determines the direction and amount of change. Green color (-3) indicates an increase after use. While red color (+3) indicates a decrease. Electrodes highlighted in blue (thick circumferential line) are 11 These are the places where statistically significant changes (Wilcoxon test) are observed. Accordingly, delta and An increase in QSE values ​​in gamma bands indicates increased complexity and disorder. It is seen. F7 and T5 electrodes are used in EEG studies, particularly due to their proximity to the hippocampal region. It is a focal point in Alzheimer's patients. High beta and low gamma levels in this region. The change in frequency, especially considering the role of gamma frequency in information processing in the brain, It carries significant meaning. In this context, the differences identified were clearly observed in the EEG tracings of the patients. Traces are presented in 10-second segments (Figure 4): Figure 4. Graphs of the first subject's 10-second resting state EEG tracing. Figure 4 shows an 88-year-old male patient diagnosed with dementia before using the developed food supplement. 10-second resting state EEG tracing (Left), horizontal axis 10 sec, vertical axis 100uV. In this way, and again, the use of the developed food supplement by an 88-year-old male patient diagnosed with dementia. EEG tracing after 10 seconds of rest (Right), horizontal axis 10 sec, vertical axis It is shown as 100uV. 12 Figure 5. Graphs of the second subject's 10-second resting state EEG tracing. Figure 5 shows an 81-year-old female patient diagnosed with dementia before using the developed food supplement. Resting state EEG tracing for seconds (Left), horizontal axis 10 sec, vertical axis 100uV. In this way, and again, the use of the developed food supplement by an 81-year-old female patient diagnosed with dementia. EEG tracing after 10 seconds of rest (Right), horizontal axis 10 sec, vertical axis It is shown as 100uV. In the trace images provided for the two patients, an increase in high frequencies was observed in the recordings after use. This can be understood from the rapid changes. In the first recording of the male patient, slow wave activity was observed. The prominence is noticeable. Additionally, alpha fluctuations are observed in the recordings after use. It will be noticed that there is an improvement in the form. The energy in the delta band decreases and the high An increase in energy at a certain frequency (gamma) is recognized as this trace by those familiar with EEG examinations. It is also seen on it. A food supplement that has an effect on cognitive functions, developed with the current invention, and the effects of this supplement on cognitive functions. Thanks to the production method, especially using black carrot and chicory roots, Alzheimer's Prevention and / or alleviation of cognitive impairment effects that occur in similar types of dementia This is ensured. In addition, its natural ingredients provide antioxidant effects and support the gut microbiota. by supporting the reduction of the risk of developing and / or progressing neurodegenerative diseases an effective, efficient and functional food that provides enhanced bioavailability and reduced toxic effects. supplementation and the food supplement production method that enables the obtainment of this food supplement. is being done.

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