A SKIN CARE SYSTEM AND ITS APPLICATION METHOD

Abstract

The invention relates to a skin care system and its application method that directly and automatically performs real-time skin detection and appropriate care, integrated with a targeted therapeutic response according to the user's skin type.

Description

A SKIN CARE SYSTEM AND ITS APPLICATION METHOD Technical Area The invention relates to a skin care system and its application method. The invention is particularly integrated with a targeted therapeutic response tailored to the user's skin type. real-time skin detection and automated treatment accordingly. It relates to the skincare system and its application method. The Invention's Infrastructure Skincare encompasses a variety of methods to maintain and improve the health and appearance of the skin. It is a comprehensive process in which various methods are applied. Its basic steps include daily cleaning, It includes moisturizing, exfoliating, and mask treatments. The most fundamental requirement for skincare is correctly identifying your skin type and determining its needs. It is the accurate identification of the contents. Skin data varies from person to person. Therefore, maintenance that is suitable for one user may not be suitable for another user. Current skin care techniques have some shortcomings, as listed below. It is located. Current skincare techniques are based on the "one size fits all" principle. It has the same features regardless of the different needs of an oily T-zone and dry cheeks. They apply the ingredients to the entire face and cannot adapt to daily skin changes. Many potent active ingredients (such as Vitamin C and Retinol) are unstable and degrade rapidly. Furthermore, the molecular sizes in traditional formulations allow for deep skin penetration. It is very large, which limits their effectiveness. 2 Consumers often misdiagnose skin conditions by self-diagnosing them. They are forced to do so. This situation results in products that are ineffective or cause further irritation. This leads to an expensive trial-and-error cycle with the products. The active ingredients are applied indiscriminately to the entire skin surface. This is inefficient and invaluable. It wastes its components on healthy skin and increases the risk of widespread irritation or sensitivity. Tretinoin (a retinoid) is used in current skincare applications. Strong and effective prescription topical products, such as hydroquinone (a skin lightening agent), have significant side effects. It is known to cause adverse effects. These typically include severe dryness and peeling, It includes redness (erythema) and inflammation. In the literature, regarding the subject in patent application number TR 2024 / 015528, it is stated as "This The invention involves proper skincare, the right product presentation, and internships for university students in this field. It relates to a skincare assistant algorithm that can be used to offer this possibility, and its feature is; User interface where users log in to the system and register their personal information, The server provides access to various plugins available on the system, allowing users to access personal information. information, skin information, product recommendations, internship applications, and live chat history data The database where it is stored allows users to connect with expert dermatologists in real time. through the live chat module with which students interact and by viewing internship announcements and applying It has an internship module where applicants can complete their internships and employers can also evaluate applications. "It is..." The application in question describes a skincare treatment performed using a skincare assistant algorithm. The application is included. Furthermore, the subject matter is discussed in the literature in patent application number TR 2024 / 012571. The invention is defined as "a contact surface that comes into contact with the skin." By having this done, a skin analysis can be performed, revealing at least one skin type. to identify the defect and to treat at least one of the identified skin defects. It relates to a medical skin care device that helps determine the treatment method. Its innovation lies in the fact that it is placed on the contact surface, and when it comes into contact with the skin, it protects the skin surface and the skin itself. at least one sensor unit that enables the measurement of at least one skin data point, skin A skin care professional trained in skin defects and treatment methods for the aforementioned skin defects. When it receives the data as input, it determines the skin defect and the treatment method for the mentioned skin defect. a mathematical model that produces an output related to the aforementioned sensor unit By applying skin data as input, the skin defect mentioned in the skin and the mentioned a processor that provides output information on the treatment method for skin defects the unit, according to the skin defect treatment method information received from the aforementioned processor unit It is characterized by containing a treatment unit that enables the treatment of the skin. "It is..." According to the information in the aforementioned application regarding the treatment method for skin defects, the skin should be treated. The package includes a skin care device that enables this process. Furthermore, the literature mentions European patent application number EP4157031B1 regarding this matter. As a "skincare system," it involves performing a treatment on the skin and the system's... a functional device configured to be moved across the skin during its operation The element and the functional element are moved together over the skin and indentations are made in the skin during the process. a measuring unit containing a measuring element configured for making a measurement It includes. The position of the measuring element can be changed in the measuring unit, and the measuring unit, the measuring element is in a default position on the measuring unit due to the skin treatment. It is configured to measure a value related to a degree whose position has been changed. Based on the measurement results generated by the measuring unit, the unit under maintenance It is possible to automatically adapt the system's operation to the type of fuselage area. These statements are included. The patent in question describes a skincare product that performs a treatment on the skin. The system is included. Furthermore, in the literature, the subject is discussed in PCT application number WO / 2019 / 097291. In this regard, "A sample skincare tool has a first side, a second side, and an intermediate section, to a trough, a light source, a motor, a temperature control unit, and a light source, to control one or more of the motor and temperature control units It includes a main body with a configured user control. Skincare tool It can apply heating, cooling, light and / or vibration therapy to a user's skin. These statements are included. 4 The application in question includes skincare tools and how to use them. is provided. Due to the disadvantages mentioned above, a new skincare system and its... There was a need to develop an implementation method. Disclosure of the Invention Based on this position in the art, the aim of the invention is to eliminate the existing disadvantages. The goal is to introduce a new skincare system that removes unwanted skin and its application method. Another aim of the invention is to provide treatment without the need for any external devices. completely biomarker-sensitive release that makes it seamless and effortless The goal is to create an automated system. Another aim of the invention is to enable real-time, personalized and accurate diagnosis. It is about creating a structure. Another aim of the invention is to preserve the active ingredients and identify a biological need. a method that allows them to penetrate deeply enough to be ready for release when applied The goal is to create a structure. Another aim of the invention is to convert your skin's needs into understandable data and automatically... a system that tracks how the treatment improves your skin health over time to place. Detailed Description of the Invention This detailed explanation aims to provide a better understanding of the innovation in the invention. This is explained with examples that will not create any limiting effect. The invention integrates a truly targeted therapeutic response tailored to the user's skin type. A skincare product that automatically detects skin types in a timely manner and provides appropriate treatment directly. Its system and feature is that it significantly increases the penetration of natural vitamins and amino acids into the skin. 5 Nano-fermented rice that provides deep hydration and brightening by increasing moisture. Biofiltrate bases automatically release their therapeutic substances when they detect a specific need in the skin. formed to release as a discharge, surfaces showing local skin inflammation. It will open in response to specific biological triggers, such as a change in pH. Passive, targeted release of powerful components without user intervention. Poly(amidoamine) (PAMAM) is positioned as a nano-carrier vehicle providing this service. Dendrite has been functionalized with ligands that bind to specific skin biomarkers, a visible color on the skin by changing the intervals that alter the forms of reflection gold nanoparticles positioned as nano-sensors that cause the change (AuNP), natural antibacterial and anti-inflammatory benefits throughout the entire application area. by creating a temporary conductive nano-network on the skin, it provides hydration levels. an optional, external impedance sensor that can improve the accuracy of the measurement MXene Nanolayer (Ti3C2Tx), the aforementioned Poly(amidoamine) (PAMAM) Therapeutic agents loaded into dendrimers are delivered in a protected and targeted manner. It is characterized by containing active payloads positioned as agents. The aforementioned skincare system significantly enhances the penetration of natural vitamins and amino acids into the skin. Nano-fermented, providing deep hydration and brightening by significantly increasing the volume. Rice biofiltrate base, when it detects a specific need in the skin, releases its therapeutic substances. automatically released, surfaces showing local skin lesions indicating inflammation. It will open in response to specific biological triggers, such as a change in pH. Passive, targeted release of powerful components without user intervention. Poly(amidoamine) (PAMAM) is positioned as a nano-carrier vehicle providing this service. Dendrite has been functionalized with ligands that bind to specific skin biomarkers, a visible color on the skin by changing the intervals that alter the forms of reflection gold nanoparticles positioned as nano-sensors that cause the change (AuNP), natural antibacterial and anti-inflammatory benefits throughout the entire application area. by creating a temporary conductive nano-network on the skin, it provides hydration levels. an optional, external impedance sensor that can improve the accuracy of the measurement MXene Nanolayer (Ti3C2Tx), the aforementioned Poly(amidoamine) (PAMAM) Therapeutic agents loaded into dendrimers are delivered in a protected and targeted manner. Active payloads positioned as agents, among the aforementioned active payloads, are dark a potent antiseptic containing glabridin that inhibits the enzyme (tyrosinase) responsible for stain formation 6 natural highlighters that help even out skin tone and soothe the skin. Licorice root extract, which has strong anti-inflammatory properties, contains the aforementioned active ingredient. among the loads, to reduce redness and provide protection against environmental damage. Saffron, which offers powerful antioxidant and anti-inflammatory benefits, has the aforementioned active loads. It reduces the appearance of fine lines and wrinkles by promoting cellular renewal. Retinol, an anti-aging substance that reduces and brightens skin tone, is one of the active ingredients mentioned. C as a vital antioxidant that supports collagen production for firmer skin. interpreting data from vitamins and the aforementioned gold nanoparticles (AuNP), The main mobile application provides a diagnosis to the user and monitors skin health over time. It consists of its elements. The invention also provides a targeted therapeutic response based on the user's skin type. Integrated real-time skin detection and appropriate care are automatically applied directly. It is a skin care system application method that uses high pressure. Nano-emulsified fermented rice biofiltrate using homogenization the creation of a base, gold sensitive to biomarkers for cosmetic colorimetric diagnostics. Synthesis of nanoparticles (AuNPs), highly charge-carrying, biomarkers Synthesis of responsive nano-dendrimer capsules, MXene nanolayers Functionalization with biocompatible polymers (Hyaluronic Acid) allows for multiple different applications. integrating a class of nanoparticles (nano-brass, AuNPs, dendrimers, and MXenes) by including the methodological steps for preparing a stable serum formulation It is the characterization of the situation. The system is completely intelligent and self-operating. After the serum diagnoses the skin... Nanocapsules target areas of your skin with a lower pH, such as in an inflamed or acne-prone region. It automatically releases its active ingredients according to specific biological signals. The system creates a visual "map" of skin health, providing precise measurements of different areas. gold to identify needs and guide the automated release process It uses nanoparticles (AuNP). The first step in the skin care system that is the subject of this invention is nano-fermented rice biofiltrate. The preparation of the base is being carried out. The serum base is a multi-step biotechnological process. It is created through a process. Accordingly; 7  Extraction: The second rinse water from high-quality Japonica rice is collected. This water is rich in starch, vitamins (B1, B3, B6), and minerals.  Fermentation: Rice water is fermented with a specific Saccharomyces cerevisiae culture. They are vaccinated and kept in a sterile, temperature-controlled bioreactor at 28°C for 48 hours. They are left to ferment. This process breaks down the starches into smaller, more bio- It breaks down into usable molecules and mixes the filtrate with amino acids and postbiotics. It enriches.  Purification and Nano-Processing: The fermented liquid is first processed to remove yeast cells and other It is passed through a 0.22 micron filter to remove particles. This purified bio- The filtrate is then subjected to high-pressure treatment at 20,000 psi for 5 passes. It is passed through a homogenizer. This mechanical stress is below 100 nm. stable with nutrient-rich vesicles of average particle size By forming a nano-emulsion, it dramatically increases penetration into the skin. The next step is the synthesis and loading of dendrimer nano-charges. This step involves creating the "smart delivery" component of the serum. It involves a rigorous process that ensures the dendrimers are correctly constructed and are pH-sensitive. to be made functional, to be loaded with specific therapeutic loads, and then it ensures its purification. Empty, activable nanocarriers in dendrimer synthesis and functionalization processes. is being created. 1. Nucleus Synthesis (Divergent Method): The process involves synthesizing an ethylenediamine core molecule. It starts with. Generation 4 (G4) PAMAM dendrimers consist of two repeating patterns four times. It is built layer by layer through a step-by-step iterative process. Michael's involvement: The core is reacted with methyl acrylate. This, It adds ester-terminated branches to the molecule. Amidenization: New ester groups then react with excess ethylenediamine. They are being reacted. This involves converting ester endings into amine-terminated branches. by converting it, the number of reactive sites on the surface of the dendrimer is halved. It folds. This two-step cycle is a Generation 4 with 64 primary amine groups on its surface. This process is repeated until a dendrimer is formed. 8 2. Functionalization with pH-Sensitive Linkers: Making the Dendrimer "Smart" A pH-sensitive bond is added to bring about this. The amine-terminated G4 dendrimer is bifunctional, such as 4-hydrazinobenzoyl chloride. It is reacted with a linker molecule. This reaction involves attaching a hydrazide group to the surface of the dendrimer. The group is stable at the skin's normal pH (5.5), but in inflamed areas. those that decompose (hydrolyze) under more acidic conditions (pH < 5.0), like those found elsewhere. It has the ability to form a hydrazone bond. This "trigger" It is the mechanism. The next step is load preparation and encapsulation: batch A (anti-aging) active The process of preparing the solution is carried out. For this purpose;  A hydro-alcoholic solvent (70% ethanol, 30% water) is prepared.  Water-soluble acetyl hexapeptide-8, at a specific concentration in this solution It is being resolved.  High-purity saffron extract (rich in crocin) is added later. And it is being resolved.  In a separate container, the oil-soluble Retinol is first mixed with a cosmetic-grade product such as Polysorbate 80. Dissolved in a small amount of auxiliary solvent and surfactant, it is dispersible in water. A micro-emulsion is formed. This is then added to the main hydro-alcoholic solution. is being added. Encapsulation Process:  Functionalized dendrimers from Step A are placed in a phosphate buffer solution. It dissolves at a pH of 7.0.  Active solution of Part A, continuous, high-speed stirring (magnetic at 800 rpm) (Using a stirrer) it is added drop by drop to the dendrimer solution.  The mixture is stored in a light-protected container (because retinol is light-sensitive) at 30°C for 12 hours. They are incubated for hours. During this time, the active molecules form hydrogen bonds and through a combination of hydrophobic interactions into the internal cavities of the dendrimer It is being pulled and tied to its branches. 9 The next step involves load preparation and encapsulation of batch b (illuminator). For this; 1. Preparation of the Active Solution: Licorice root extract (containing glabridin) is soluble in deionized water. L-Ascorbic Acid (Vitamin C) is then carefully added to the same solution. It is being dissolved. The pH of this solution ensures that Vitamin C remains stable and to prevent premature triggering of pH-sensitive binders on dendrimers to provide immediate ~6.0 by using a buffering agent It is being adjusted. 2. Encapsulation Process: The active solution of Part B is separately functionalized under continuous mixing. It is added to the dendrimer batch drop by drop. The mixture is incubated at 30°C for 12 hours. Encapsulation is primarily performed in this way. by hydrogen bonds between the active ingredients and the interior of the dendrimer It is being redirected. In the critical final step, dialysis, the purification process successfully extracted the unencapsulated active ingredient. It removes the components. 1. Membrane Dialysis: Each batch (A and B) is loaded into a separate dialysis bag. This the bag has a specific Molecular Weight Cut-off Point (MWCO) of approximately 10 kDa. It is made of a semi-permeable membrane. 2. Purification Process: Sealed bags are placed in a large container of fresh buffer solution. It is being immersed. Small, unencapsulated active molecules (Retinol, Vitamin C, etc.) It can pass through the pores of the membrane and the surrounding buffer. Much larger dendrimer-load complexes inside the bag He is trapped. 3. Tampon Change: The external tampon should be fresh every 6 hours for a period of 24 hours. It is replaced with a batch. This preserves a concentration gradient. by providing all free-floating, unencapsulated active ingredients effectively. It enables its removal. 10 4. Final Product: The purified solutions inside the dialysis bags are now available exclusively. It contains "smart" payload-carrying dendrimers and is ready for the final serum formulation. 3. Synthesis and Functionalization of Gold Nanoparticles (AuNPs) for Diagnostic Purposes: This process is divided into two critical stages: First, the nanoparticles themselves... the creation of "smart" devices that enable them to detect skin biomarkers. Bonding molecular sensors to surfaces. Synthesis using the A. Turkevich Method. This classical method produces the characteristic ruby ​​red color. to create a stable, uniform gold nanoparticle suspension (colloid) in its color It is used. Preparation of Reagents:  Gold precursor, tetrachloroauric acid (HAuCl4) solution, high purity, deionized It is dissolved in water to achieve a final concentration of 1 mM.  The reducing agent, trisodium citrate (Na3C6H5O7) solution, is deionized separately. It is prepared in water at a concentration of 34 mM. Reaction Procedure:  Approximately 100 mL of HAuCl4 solution, with a condenser to prevent condensation. a round one equipped and placed in a heating mantle with a magnetic stirrer It is attached to a balloon with a base.  The pale yellow gold solution is heated vigorously, to the boiling point, while being continuously stirred. It is heated.  Once it starts boiling, add approximately 10 mL of sodium citrate solution to the flask. It is injected rapidly.  The solution is kept boiling while being stirred. Color change, nanoparticles It is a key indicator of its formation and growth: The solution first changes from yellow to colorless when the Au3+ ions are reduced. Then, when the gold atoms began to nucleate into tiny particles It darkens to a grayish-blue color for a short time. Within 1-2 minutes, the color transforms into a bright, clear ruby ​​red. stable, monodisperse nanoparticles with a diameter of approximately 20 nm This shows that it has formed. 11  To ensure the reaction is complete, let the solution sit for another 15 minutes. It is allowed to boil, then slowly cooled to room temperature. Basic Chemistry:  Reduction: Citrate ion reduces gold ions (Au3+) to neutral gold atoms (Au0). It functions as a reducing agent, donating electrons to reduce the substance.  Nucleation and Growth: Neutral gold atoms are unstable and rapidly form small "seeds" or they combine to form nuclei, which then become larger. It transforms into nanoparticles.  Stabilization (Capping): Excess citrate ions trap the newly formed gold. It adsorbs onto the surface of nanoparticles. This creates a strong negative charge on each particle. This gives them a surface charge, which causes them to repel each other and clump together. This electrostatic repulsion prevents (clumping) of the colloidal solution. It ensures it stays put. B. Functionalization with Biomarker-Sensitive Ligands: This step involves functionalizing generic AuNPs. It transforms them into highly specific nano-sensors. Design of Thiole-Bound Ligands: The "Smart" ability is a special feature with three main components. It comes from engineered molecules:  Thiol Group (-SH): An "anchor" at one end of the molecule. Sulfur atom, gold It forms a strong, stable covalent bond (Au-S bond) with the nanoparticle surface.  Intermediate Chain (PEG): A flexible chain, such as polyethylene glycol, extending from the anchor. Sensor prevents the portion from crowding on the surface and the final serum of the nanoparticle It increases the stability of the formula.  Biorecognition Head Group ("Sensor"): The active part at the other end of the molecule, It is designed to interact with a specific skin biomarker. For example: For pH sensing: It changes its charge and shape at low pH. color by altering the electrostatic balance between nanoparticles A molecule containing a specific sequence of amino acids that causes it to slip. For enzyme detection: Specifically, those secreted by P. acnes bacteria. A short peptide sequence that is a known substrate for enzymes. Bacterial. When the enzyme is present, it cleaves this peptide, which then removes the head group. by causing the nanoparticle 12 to separate or change shape aggregation and a noticeable color change from red to blue / purple Why does that happen? Ligand Exchange Procedure:  Specially designed thiol-bound ligands are dissolved in a suitable buffer.  This ligand solution is slowly added to the ruby ​​red AuNP colloid while stirring.  The mixture is incubated in a dark environment at room temperature for 24 hours. This period In the case of thiol groups on new ligands, the value for gold is much higher. weaker bound citrate on the nanoparticle surface because of their affinity It displaces its ions. Purification:  After incubation, remove the displaced citrate ions and excess, Remove unbound ligands.  This is achieved with several centrifugation cycles. The solution is spun at high speed. heavier, functionalized AuNPs form a pellet at the bottom of the tube It is provided.  The supernatant (the liquid containing the contaminants) is carefully removed and the AuNP pellet is placed in a clean container. It is resuspended in buffer. This process takes 3-4 minutes to ensure high purity. This process is repeated several times. The final product is a highly specific, biomarker-sensitive nano- It is a stable colloid for sensors. 4. Functionalization of MXene Nanolayers: This process involves a bulk, layered structure. ceramic material, safe and stable for use in a cosmetic formulation, It transforms into a functional, single-layer nanomaterial. A. Exfoliation: From 3D MAX Phase to 2D MXene. The purpose of this step is to prepare the ceramic selectively removing the metallic "adhesive" that holds the layers together, and then this It is about separating the layers. Starting Material (MAX Phase): The process uses the MAX phase with the chemical formula Ti3AlC2. It starts with a specific ceramic powder called [name of material]. This material is unique, nano-laminar. It has a crystalline structure. The "bread" and metallic layers of titanium carbide (Ti3C2) Imagine a repeating sandwich where a layer of aluminum (Al) is the "filler". This The aluminum layer is chemically weaker than the titanium carbide layers, and It is more reactive. Selective Etching (Removing "Filler"):  Setup: The reaction, the acidic solution, is highly corrosive to glass and metal. Therefore, it is carried out in a polytetrafluoroethylene (PTFE) or Teflon beaker. 13 The beaker is an ice pack with a magnetic stirrer to control the reaction temperature. It is placed in the bathroom.  Procedure: A hydrochloric acid (HCl) solution is prepared. Lithium fluoride (LiF) is then added. Then it slowly dissolves into the acid. Finally, the Ti3AlC2 powder undergoes an exothermic reaction. and very slowly for about 10 minutes to manage hydrogen gas production. and is carefully added to the acidic solution.  Reaction: The mixture is sealed and stirred at a constant 35°C for 24 hours. is left in place. During this time, the acid selectively attacks the aluminum layers and By dissolving them, it forms soluble aluminum chloride (AlCl3), which is then washed away.  Chemical Transformation: As aluminum is extracted, new layers of titanium carbide are formed. the resulting surfaces are primarily connected to functional groups from the solution. The material is "terminated" with oxygen (-O), hydroxyl (-OH), and fluoride (-F). It is not Ti3AlC2; now "Tx" represents these various surface terminations. It is a multilayered, accordion-like structure called Ti3C2Tx. Delamination (Separation of Layers):  Washing: After etching, the material is a dark mud. It is extremely acidic and It should be washed to remove toxic byproducts. This involves adding deionized water, solids. centrifuging the mixture to collect the substance and discarding the supernatant in cycles This process is repeated until the pH of the water is neutral (pH 6-7).  Sonication: The washed, multilayered MXene powder is then immersed in a solvent (this In this case, deionized water is suspended. This suspension is placed in an ultrasonic bath. or a probe is subjected to sonication with a sonicator. High-frequency sound. The waves create intense, microscopic cavitation bubbles. These bubbles Its formation and collapse are powerful shocks that separate the weakly connected layers of the "accordion". It produces waves.  Final Product: Sonication and removal of any unexfoliated material. After a final centrifugation step, the result is single or multi-layered Ti3C2Tx. It is a stable, dark greenish-gray colloidal suspension of nanosheets. B. Coating with Biocompatible Polymers This step involves preparing nanosheets for cosmetic use. It makes it safer, more stable, and even more beneficial. 1. Purpose of the Coating: 14 o Biocompatibility: The primary goal is to create a bond between the inorganic nanolayer and the skin. by creating a soft, biocompatible interface The goal is to minimize the potential for irritation. Stability: Polymer coating, final, complex serum of nanolayers. by preventing oxidation or recombination in its formula It ensures that it remains disassembled and functional. Additional Benefit: The selected polymers themselves are excellent moisturizing agents. 2. Coating Procedure (Electrostatic Self-Fusing): Preparation of Polymer Solution: Sodium alginate and low molecular weight Separate dilute aqueous solutions of hyaluronic acid are prepared. Both of them They are long-chain polymers with negative charges (due to carboxyl groups). Coating: Delaminated MXene suspension with vigorous mixing. It is placed underneath. Sodium alginate and hyaluronic acid solutions are then applied. They are added slowly and sequentially. The mechanism: Long polymer chains, hydrogen bonds, and other molecules. MXene nanosheets through a combination of intermolecular forces It is attracted to the surface. By surrounding individual nanolayers, it forms a stable, hydrated environment. They form a "shell". The mixture is used to ensure a complete and homogeneous coating is formed. It is left to be stirred for a few hours. 3. Purification and Final Product: The excess, unbound polymer remaining in the solution, now heavier A final, gentle centrifugation to separate the coated MXene nanosheets. The step is used. The final product is integrated as a functional component into AuraDerm serum. ready-to-use, biocompatible, polymer-coated Ti3C2Tx nanosheets It is a purified, stable suspension. 5. "AuraDerm" Serum Final Formulation  Base Preparation: In the main formulation vessel, Nano-Fermented Rice Biofiltrate base It is heated slightly and kept at 40°C.  Addition of Free Actives: Niacinamide, Allantoin, and Ferulic Acid to warm base It is added and stirred until completely dissolved. 15  Integration of Nanoparticles: To avoid damaging the nanoparticles For maximum stability under controlled, low shear force mixing. The following suspension components are added in a specific order: 1. Coated MXene nanolayer suspension. 2. Purified Dendrimer Part A (Anti-Aging). 3. Purified Dendrimer Batch B (Illuminating). 4. Functionalized AuNP suspension.  Finishing: A gelling agent is used to achieve the desired serum viscosity. Xanthan gum is dispersed. The pH of the final product is carefully adjusted using triethanolamine. It is adjusted to 5.8. Before the serum is filled into airtight, UV-protected packaging... Finally, it undergoes a gentle homogenization.  Nano-Fermentation: Fermentation of rice water between 25°C and 32°C. At a controlled temperature, it can last from 40 to 55 hours.  High-Pressure Homogenization: Fermented biofiltrate to target 50 to 150 nm. To obtain the average particle size, a pressure between 15,000 and 25,000 psi is required. It can be processed under pressure for 3 to 7 passes.  Dendrimer Encapsulation: Incubation of active ingredients with dendrimer solutions, At a temperature between 25°C and 35°C, this can take 10 to 16 hours.  MXene Etching: Selective etching of MAX phase powder, between 30°C and 40°C. This can be done at a maintained temperature for 20 to 28 hours.  Final Serum Formulation: Integration of Nanoparticle Components During this process, the serum base should be maintained at a temperature between 35°C and 45°C. The product's final pH can be adjusted to a skin-compatible range between 5.5 and 6.2. Contributions and Properties of Elements  Nano-Fermented Rice Biofiltrate (Base Base): This high-performance active ingredient The base is produced by converting fermented rice water into vesicles smaller than 100 nm. It is formed through nano-processing. This allows natural vitamins and amino acids to penetrate the skin. It provides deep hydration and brightening by significantly increasing the intensity of the effect.  PAMAM Dendrimers (Nano-Carrier Devices): These deliver a specific material to the skin that meets a particular need. to automatically release their therapeutic agents when they detect them They are the 'smart couriers' of the designed system. Their surfaces show local 16% inflammation. It will open in response to specific biological triggers, such as a change in skin pH. It is designed in such a way that powerful components operate without user intervention. It enables passive, targeted release.  Gold Nanoparticles (AuNPs) (Nano-Sensors): These are used in the diagnostic system. It is the heart of the matter. It is functionalized with ligands that bind to specific skin biomarkers. This bonding event alters the intervals in the skin, changing the forms of reflection. It causes a visible color change.  MXene Nanolayers (Ti3C2Tx) (Functional Support Material): This 2D Nanomaterials have two critical roles: natural antibacterial and anti-inflammatory benefits throughout the entire application area It creates a temporary conductive nano-network on the skin, which in turn regulates hydration levels. It can improve the accuracy of an optional, external impedance sensor used for measurement.  Active Payloads: These are protected and targeted payloads loaded into the dendrimers. These are therapeutic agents delivered in some way. The main payloads include: Licorice Root Extract: the enzyme responsible for the formation of dark spots It is a powerful natural brightening agent containing glabridin, which inhibits tyrosinase, the skin. It helps to even out the skin tone. It also has a powerful anti-inflammatory effect to soothe the skin. It has inflammatory properties. Saffron: Reduces redness and provides protection against environmental damage. It offers strong antioxidant and anti-inflammatory benefits. o Retinol: Supports cellular renewal, reducing fine lines and wrinkles. It is a gold standard ingredient for anti-aging, reducing its appearance. Vitamin C: Brightens skin tone and stimulates collagen production for firmer skin. It is a vital antioxidant that provides support.  Mobile Application: This is the "brain" of the system. It processes data from the nano-sensors. The reviews provide the user with a diagnosis and track skin health over time.

Claims

1. The invention integrates a targeted therapeutic response based on the user's skin type. Real-time skin detection and appropriate care directly and automatically. It is a skincare system that uses natural vitamins and amino acids to nourish the skin. Significantly increasing its penetration for deep hydration and brightening. A nano-fermented rice biofiltrate base that provides protection by identifying a specific need in the skin. They are designed to automatically release their therapeutic substances when they are used, their surfaces, specific changes in local skin pH that indicate inflammation User intervention to activate in response to biological triggers Nano enables passive, targeted release of powerful components without the need for a nano-device. Poly(amidoamine) (PAMAM) dendrite positioned as a carrier medium, Reflective media functionalized with ligands that bind to specific skin biomarkers. changing their shapes and altering their intervals causes a visible discoloration on the skin. gold nanoparticles (AuNPs) positioned as nano-sensors, causing this natural antibacterial and anti-inflammatory benefits throughout the entire application area It provides hydration by creating a temporary conductive nano-network on the skin. an optional, external impedance sensor to measure levels The MXene Nano layer (Ti3C2Tx), which can improve its accuracy, is mentioned. Poly(amidoamine) (PAMAM) dendrimers are loaded with protected and target-sensitive materials. active payloads positioned as therapeutic agents delivered in a targeted manner It is characterized by its inclusion.

2. A skin care system that complies with Claim 1, and its characteristic feature is the aforementioned gold. interpreting data from nanoparticles (AuNPs), providing the user with a diagnosis. It includes a mobile application that monitors skin health over time.

3. A skin care system that complies with Claim 1, characterized by the fact that the aforementioned active load is applied to dark skin. Glabridin contains a compound that inhibits the enzyme (tyrosinase) responsible for the formation of spots. A powerful natural highlighter that helps to even out skin tone. Licorice root extract has strong anti-inflammatory properties to soothe. It includes. 18 4. A skin care system that complies with Claim 1, characterized by the fact that the aforementioned active load, Powerful for reducing redness and providing protection against environmental damage. It contains saffron, which offers antioxidant and anti-inflammatory benefits.

5. A skin care system that complies with Claim 1, characterized by the fact that the aforementioned active load, By promoting cellular regeneration, it reduces the appearance of fine lines and wrinkles. It contains retinol, which reduces and combats aging.

6. A skin care system that complies with Claim 1, characterized by the fact that the aforementioned active load is applied to the skin. vital for brightening skin tone and supporting collagen production for firmer skin. It contains vitamin C, which acts as an antioxidant.

7. The invention integrates a targeted therapeutic response based on the user's skin type. Real-time skin detection and appropriate care directly and automatically. It is a skin care system application method that uses high pressure. Nano-emulsified fermented rice biofiltrate using homogenization the creation of a base sensitive to biomarkers for cosmetic colorimetric diagnostics. Synthesis of gold nanoparticles (AuNPs), highly charge-bearing, Synthesis of nano-dendrimer capsules sensitive to biomarkers, MXene nanosheets with biocompatible polymers (Hyaluronic Acid) functionalization of multiple different classes of nanoparticles (nano-brass, A stable serum integrating AuNPs, dendrimers, and MXenes. It is characterized by including the methodological steps for preparing the formulation. It is done.

8. A skin care system application method that complies with claim 7, and its characteristic feature is; The process of creating the aforementioned nano-fermented rice biofiltrate base; High-quality Japonica rice contains starch, vitamins (B1, B3, B6), and minerals. Extraction by collecting the second rinse water, which is rich in nutrients. processing makes the starches smaller and more bioavailable. to break down molecules and enrich the filtrate with amino acids and postbiotics the aforementioned rice water with a specific Saccharomyces cerevisiae culture vaccination and sterilization in a temperature-controlled bioreactor at 28°C for 48 hours By allowing it to ferment, the fermented liquid first separates the yeast cells and then the other 19 Passing the treated material through a 0.22 micron filter to remove particles is the aforementioned purification process. The biofiltrate is then subjected to high-pressure treatment at 20,000 psi for 5 passes. Passed through a homogenizer to achieve an average particle size below 100 nm. a stable nano-emulsion containing nutrient-rich vesicles It includes the steps involved in its creation.

9. A skin care system application method that complies with claim 7, and its characteristic feature is; In the process of synthesizing the aforementioned nano-dendrimer capsules, Divergent The method involves synthesizing a core molecule by esterifying it with an ethylenediamine core molecule. to add finite branches, the ethylenediamine core molecule with methyl acrylate reacting them by converting ester endings into amine-terminated branches A new ester doubles the number of reactive sites on the dendrimer surface. Amitization by reacting the groups with excess ethylenediamine The process involves the aforementioned two-step cycle, with 64 primary amines on its surface. The method involves repeating the process until a Generation 4 dendrimer containing a group is formed. It includes the steps.

10. A skin care system application method in accordance with claim 9, characterized by: To make the aforementioned dendrimer smart, a pH-sensitive bond is added, and like those that are stable at the skin's normal pH (5.5), but are found in inflamed areas a hydrazone bond that breaks down (hydrolyzes) under more acidic conditions (pH < 5.0) Amin finite G4 to enable it to create dendrimer, a bifunctional linker molecule such as 4-hydrazinobenzoyl chloride. reacting with it results in a deposit being formed on the surface of the dendrimer. It involves the steps of the hydrazide group binding method.

11. A skin care system application method in accordance with claim 9, characterized by its part a. The process of preparing the (anti-aging) active solution involves a hydro-alcoholic Preparation of solvent (70% ethanol, 30% water), water-soluble acetylhexapeptide-8 Dissolving in solution, adding and dissolving high-purity saffron extract, In a separate container, fat-soluble retinol is first mixed with a cosmetic-grade product such as Polysorbate 80. Dissolved in a small amount of auxiliary solvent and surfactant, it is dispersible in water. Formation of micro-emulsion and its addition to the main hydro-alcoholic solution, Functionalized dendrimers were dissolved in a phosphate buffer solution (pH 7.0), 20 The active solution of part A was subjected to continuous, high-speed stirring (magnetic at 800 rpm). (using a stirrer) adding the dendrimer solution drop by drop, The mixture should be incubated in a light-protected container at 30°C for 12 hours; this period within it, active molecules exhibit a series of hydrogen bonds and hydrophobic interactions. by being drawn into the internal cavities of the dendrimer through combination and branching The method involves encapsulating the material by means of binding.

12. This is a skin care system application method that complies with claim 9 and is characterized by: encapsulation batch b (illuminating) process; licorice root extract (glabridin (containing) L-Ascorbic Acid (Vitamin C) is dissolved in deionized water, in the same solution. Careful dissolution, the pH of the solution in question, ensures the stability of Vitamin C. and to prevent premature triggering of pH-sensitive binders on dendrimers To ensure this, it is immediately set to ~6.0 using a buffering agent, obtained The active solution of batch b obtained was separately functionalized under continuous stirring. Adding the mixture dropwise to the dendrimer batch, incubating the mixture at 30°C for 12 hours. primarily by breaking the hydrogen bonds between the active ingredients and the interior of the dendrimer. encapsulation method steps to be guided by It includes.

13. A skin care system application method that complies with claim 11 or 12, and its characteristic is; purification process using dialysis to extract unencapsulated active ingredients A specific molecular weight of approximately 10 kDa was determined separately for each batch (a and b). Dialysis through a semipermeable membrane with a medium breakpoint (MWCO). loading into bags, sealed bags into a large container of fresh buffer solution Dipping, small, unencapsulated active molecules (Retinol, Vitamin C, etc.) the buffer in question can pass through the pores of the membrane its enclosure, much larger dendrimer-charge complexes inside the bag Containment ensures the preservation of the concentration gradient, thereby releasing all free fluid. To ensure the efficient extraction of floating, unencapsulated active substances. The aforementioned external tampon should be freshened every 6 hours for a period of 24 hours. Replacing one batch with another, the purified solutions inside the dialysis bags, now to include only "smart" payload-carrying dendrimers and the final serum The formulation includes the methodological steps for preparing the formulation. 21 14. A skin care system application method that complies with Claim 7, and its characteristic feature is; gold. The process of synthesizing nanoparticles (AuNPs) has a characteristic ruby-like appearance. a stable, uniform suspension (colloid) of gold nanoparticles with a reddish color To form the gold precursor, a high-grade solution of tetrachloroauric acid (HAuCl4) in pure, deionized water to achieve a final concentration of 1 mM, The reducing agent, trisodium citrate (Na3C6H5O7) solution, was separately deionized. Preparation of reagents at a concentration of 34 mM in water, approximately 100 mL The HAuCl4 solution is placed in a container equipped with a condenser to prevent condensation, and a round-bottomed balloon placed in a heating mantle with a magnetic stirrer the addition of the pale yellow gold solution, while being constantly stirred, causes a violent boiling. heating to the point where it starts to boil, then adding approximately 10 mL Sodium citrate solution is rapidly injected into the balloon while the solution is being mixed. Keeping it boiling is to ensure the reaction is complete. Allow the solution to boil for another 15 minutes, then slowly cool it to room temperature. It includes the cooling method steps.

15. A skin care system application method that complies with claim 14, characterized by its generic nature. To convert AuNPs into specific nano-sensors sensitive to biomarkers. The functionalization process with ligands involves a thiol group (-SH) molecule With an "anchor" at its tip, a sulfur atom, on the surface of a gold nanoparticle, it is strong and stable. Formation of covalent bonds (Au-S bonds), such as polyethylene glycol extending from the anchor. The sensor portion of the intermediate chain (PEG), which is a flexible chain, is on the surface. by preventing crowding of the nanoparticles in the final serum formula increasing its stability, biorecognition, the active site at the other end of the molecule. The sensor group interacts with a specific skin biomarker to detect pH. for; nanoparticles that change their charge and shape at low pH, between them a specific amino acid that causes color shift by altering electrostatic balance A molecule containing an acid sequence is specifically targeted by enzyme detection in P. acnes bacteria. a short peptide sequence that is a known substrate for enzymes secreted by, When a bacterial enzyme is present, it cleaves this peptide, removing the head group. by causing separation or deformation of nanoparticle aggregation and the steps involved in a distinct color change from red to blue / purple It includes. 22 16. A skin care system application method that complies with claim 14, characterized by its generic nature. To convert AuNPs into specific nano-sensors sensitive to biomarkers. The functionalization process with ligands, thiol-bound ligands are suitable. Dissolving in buffer, this ligand solution turns ruby ​​red AuNP when stirred. Slowly adding the mixture to the colloid, allowing the mixture to sit at room temperature in a dark environment for 24 hours. After an hour of incubation, the thiol groups on the new ligands become very similar to gold. because they have a higher affinity, they adhere more closely to the nanoparticle surface. displacing weakly bound citrate ions, after incubation, its removal of extracted citrate ions and excess, unbound ligands, all at once by spinning the solution at high speed with multiple centrifugation cycles, it becomes heavier. The functionalized AuNPs form a pellet at the bottom of the tube, the supernatant The liquid containing the contaminants is carefully removed and the AuNP pellet is placed in a clean buffer. Resuspending it, this process requires 3-4 times to ensure high purity. by repeating the process, highly specific, biomarker-sensitive nano-sensors The method involves steps to produce a stable colloid.

17. A skin care system application method compliant with Claim 7, characterized by its MXene properties. nanolayers with biocompatible polymers (Hyaluronic Acid) the functionalization process of a bulk, layered ceramic material, a safe and stable, functional, single-use product for use in a cosmetic formulation to transform ceramic layers into a layered nanomaterial selective removal of the metallic adhesive holding them together, and then this The separation of layers, the reaction, the acidic solution is quite suitable for glass and metal. Because it is corrosive, it should be placed in a polytetrafluoroethylene (PTFE) or Teflon beaker. This is achieved by using a magnetic field in the beaker to control the reaction temperature. Placing the hydrochloric acid (HCl) solution in an ice bath with a stirrer. preparation, then slowly dissolving lithium fluoride (LiF) in acid, Managing the exothermic reaction of Ti3AlC2 powder and hydrogen gas production. for about 10 minutes, very slowly and carefully, into the acidic solution the mixture was sealed and left to stand at a constant 35°C for 24 hours. By allowing it to be stirred, the acid selectively coats the aluminum layers. by attacking and dissolving them, soluble aluminum chloride (AlCl3) is washed away As aluminum is extracted, new layers of titanium carbide are formed. the resulting surfaces are in contact with functional groups from the solution, primarily oxygen. Termination with (-O), hydroxyl (-OH) and fluoride (-F), addition of deionized water, solid. centrifuging the mixture to collect the substance and discarding the supernatant in cycles washing, this process is repeated until the pH of the water is neutral (pH 6-7), The washed, multilayered MXene powder is then placed in a solvent (in this case (deionized water) is suspended, and this suspension is then placed in an ultrasonic bath or a probe being subjected to sonication with a sonicator, high-frequency sound waves the formation of dense, microscopic cavitation bubbles, these bubbles the formation and collapse of the accordion's weakly bonded layers with a powerful shock generating waves, sonication and any unexfoliated material After a final centrifugation step to remove the layers, the result is a single or multi-layered molecule. Stable, dark greenish-gray colloidal suspension of Ti3C2Tx nanosheets. It includes the steps involved in obtaining it.

18. A skin care system application method in accordance with claim 17, characterized by: making nanosheets safe, stable and even more beneficial for cosmetic use coating process with biocompatible polymers to bring about, sodium alginate and separate dilute aqueous solutions of low molecular weight hyaluronic acid Preparation of delaminated MXene suspension, vigorous mixing. placed underneath, sodium alginate and hyaluronic acid solutions were slowly and sequentially added, long polymer chains, hydrogen bonds and other molecules on the surface of MXene nanosheets through a combination of interstitial forces by being drawn, it surrounds the individual nanolayers, forming a stable, hydrated "shell". the formation of the mixture, ensuring that a complete and homogeneous coating is formed. left to stir for a few hours, removing any excess, unbound residue remaining in the solution. Separating the coated MXene nanosheets, which are now heavier, from the polymer. The method involves several steps, including the use of a final, gentle centrifugation step.

19. Applying a skin care system that conforms to any of the claims from 7 to 18. The method and its characteristic feature is that in the main formulation vessel, Nano-Fermented Rice Bio- The filtrate was slightly heated and kept at 40°C, and Niacinamide, Allantoin and Ferulic were added. Add the acid to the lukewarm base and stir until completely dissolved, 24 Controlled, low shear force to avoid damaging nanoparticles. MXene nanolayer coated for maximum stability under stirring. suspension, purified dendrimer part a (Anti-Aging), purified dendrimer part b (illuminating) and functionalized AuNP suspension a gelling agent is added sequentially to achieve the desired serum viscosity. Xanthan gum is dispersed, and the pH of the final product is adjusted using triethanolamine. Carefully adjusting the setting to 5.8 ensures the final serum is packaged in airtight, UV-protected containers. a final gentle homogenization before filling method It includes the steps.