HAIR LOSS PREVENTION FORMULATION AND PRODUCTION METHOD CONTAINING CHASTEBERRY LEAF EXTRACT

Abstract

The invention aims to obtain a hair loss preventive extract from plant material. - Mixing the plant material with the solvent to increase the contact surface area with the solvent phase, thereby initiating the migration of target phytochemical compounds into the solvent environment. - Ultrasonic processing of the mixture to increase the extraction efficiency of phenolic and flavonoid compounds by weakening the cell wall integrity through cavitation effect, - Simultaneous application of light during extraction to reduce the degradation of active compounds by suppressing oxidative enzyme activity and to increase extract stability. - separation of the extract at the end of the process to ensure the obtainment of the phase enriched with active components, This relates to the production method of a formulation containing a chaste tree leaf extract, including the steps involved.

Description

HAIR LOSS PREVENTION PRODUCT CONTAINING CHASTEBERRY LEAF EXTRACT. FORMULATION AND PRODUCTION METHOD TECHNICAL FIELD The invention relates to the technical field of cosmetics and pharmaceutical preparations; specifically: Hair loss, especially androgenetic alopecia, hair follicle miniaturization, scalp inflammation, and dermal papilla function an effective herbal formulation against disorders and this formulation It relates to the production method. The invention also involves the preparation of Vitex agnus-castus L. leaves using natural solvents. Standardized extracts obtained by ultrasonic and light-assisted extraction of hair It is intended for use in preparations. PREVIOUS TECHNIQUE Hair loss, particularly androgenetic alopecia, affects both men and women. commonly seen in the population and significantly affecting the quality of life of individuals It is a problem that affects people. Nowadays, this condition is commonly treated. Synthetic active ingredients such as minoxidil and finasteride are used. However, the word Long-term use of these agents may cause local irritation, hormonal side effects, and Undesirable side effects such as systemic tolerability problems may occur; this situation patient compliance, especially in cases requiring long-term and continuous use. It can reduce it. While plant-based products are presented as safer alternatives, There are serious technical shortcomings in the current applications. These products... Most of the herbal extracts used are not standardized, and the active ingredient... Contents may vary from batch to batch. Extraction methods that it is not scientifically optimized and controlled, This negatively affects repeatability and the consistency of product quality. Furthermore... The quantity and stability of biologically active compounds such as phenolic and flavonoid compounds are mostly... 1. Time is not being analyzed or controlled. As a result, predictable and reliable results in terms of bioavailability and therapeutic efficacy. This is not possible. This situation indicates that herbal products are clinically potent and This limits its ability to create a sustainable alternative. In conclusion, all the problems mentioned above require an innovation in the relevant technical field. This has made it necessary to do it. A BRIEF DESCRIPTION OF THE INVENTION The present invention eliminates the aforementioned disadvantages and the related technical To bring new advantages to the field, hair loss treatment containing chaste tree leaf extract. It is related to preventive formulation. The main goal of the invention is to develop a formulation (extract) that prevents hair loss. to place. Another aim of the invention is to create a product containing a chasteberry leaf extract that strengthens hair follicles. The goal is to produce a formulation (extract). Another aim of the invention is to support dermal papilla function using a chasteberry leaf. The goal is to develop a formulation and production method that includes the extract. Another aim of the invention is to have a standardized and repeatable production method. The goal is to develop a formulation containing chaste tree leaf extract. Another aim of the invention is to develop a chasteberry that exhibits antioxidant and anti-inflammatory effects. The aim is to develop a formulation and production method containing leaf extract. Another aim of the invention is to develop an environmentally friendly chasteberry leaf using natural solvents. The goal is to develop a formulation and production method that includes the extract. 2. All the purposes mentioned above and those that will emerge from the detailed explanation below. The present invention aims to achieve a hair loss prevention device from plant material. for the purpose of obtaining an extract; - increasing the contact surface area between the target phytochemical compounds and the solvent phase to initiate the transfer of plant material to the environment with the solvent mixing, - by weakening the cell wall integrity through cavitation effect, phenolic and to increase the extraction yield of flavonoid compounds in the mixture ultrasonic processing, - by suppressing oxidative enzyme activity, it prevents the degradation of active compounds. to reduce and increase extract stability during extraction Timed light application, - to ensure the obtainment of a phase enriched with active ingredients separation of the extract at the end of the process, It is a production method for a formulation containing a chaste tree leaf extract, involving the following steps. Accordingly, the characteristic of the invention in question is that the plant material is Vitex agnus-castus L. (chaste tree) leaves and the leaves of Vitex agnus-castus L. (chaste tree) in question for extraction the following a. Solvent-biomass contact of Vitex agnus-castus L. (chaste tree) leaves To increase the diffusion rate by optimizing the ratio 1:4–1:6 (w / v) mixing with solvent in a certain ratio, b. To increase cell wall permeability by creating controlled cavitation and to maximize active compound release in the 18–22 kHz frequency range and subjected to ultrasonic treatment in the power range of 350–450 W, c. to prevent thermal degradation of heat-sensitive phytochemical compounds processing at a temperature range of 25–30 °C for a period of 30–60 minutes d. to suppress oxidative enzyme activity and phenolic and flavonoid compounds To increase stability, a wavelength of 430–470 nm is used during extraction. blue light application in the height range It includes the steps of the process. 3 A preferred configuration of the invention is cold pressing as the solvent medium in step a. The method involves using pumpkin seed oil obtained through a specific process. Another preferred configuration of the invention uses apple as the solvent medium in step a. It involves using vinegar. Another preferred formulation of the invention is that the pumpkin seed oil in question, The fatty acid composition consists of palmitic acid 10–16%, stearic acid 3–7%, and oleic acid 18–38%. linoleic acid should be in the range of 40–62% and linolenic acid at most 1–2%. is selected. Another preferred configuration of the invention is apple as a solvent. The pH value of the vinegar should be between 2.5 and 3.5. DETAILED DESCRIPTION OF THE INVENTION In this detailed description, the subject of the invention is only the formulation containing chaste tree leaf extract. will not create any limiting effect on a better understanding of the subject This is explained with examples. The invention relates to the hair loss preventative and hair growth properties of Vitex agnus-castus L. (chaste tree) leaves. It is based on the phytochemical potential of chaste tree, which supports follicle biology. leaves contain phenolic compounds, flavonoids, and various biologically active secondary compounds. rich in metabolites, and these compounds can be subjected to controlled and reproducible processes. The aim is to obtain it in this way. For this purpose, chaste tree leaves are optimized. on a modified, ultrasonically assisted and light-integrated extraction platform They are processed; thus, active compounds are recovered in high yield, oxidative It is possible to limit degradation and ensure standardization. is happening. The extraction process takes place at a frequency range of 18–22 kHz and a power range of 350–450 W. The procedure is performed using an ultrasonic system with a probe type. The preferred method is... In all 4 applications, extraction is performed at a frequency of 20 kHz and a power of 400 W. The plant... The ratio of material to solvent is adjusted between 1:4 and 1:6 (w / v), and the processing time is... It is applied for 30–60 minutes and the temperature is controlled between 25–30 °C. It is kept under control. In the preferred application, the ratio of plant material to solvent is 1:5. It is specified as (w / v). Also, preferably, the processing time is 45 minutes and the temperature is 30°C. It is fixed at a temperature not exceeding °C. Ultrasonic treatment is preferably performed in pulsed mode. This process is being carried out in order to optimize the cavitation effect and heat accumulation. is minimized. Throughout the process, preferably in the 430–470 nm wavelength range. A 450 nm LED blue light source is integrated into the system with controlled intensity. This practice is being carried out by increasing cell wall permeability and thus releasing phenolic and flavonoid compounds. it facilitates the passage of compounds to the solvent phase and increases oxidative enzyme activity. It is believed that suppressing this improves extract stability. This application... The total phenolic content of the resulting oil-based extract is approximately The oxygen saturation ranges from 80–200 mg / L, while the total flavonoid content ranges from 60–150 mg / L. It is standardized. The total amount of vinegar-based extract obtained by this method... The phenolic compound content is approximately in the range of 1700–2200 mg / L, and the total flavonoid content is... However, it is standardized to be in the range of 650–900 mg / L. Extraction of chaste tree leaves is done using two different application approaches. This is being carried out. In one application, it is obtained as a solvent by the cold pressing method. processed pumpkin seed oil is used, and the fatty acid content of said oil... The composition preferably consists of palmitic acid 10–16%, stearic acid 3–7%, oleic acid 18–38%, linoleic acid The percentages of acid are selected to be between 40–62%, and linolenic acid at a maximum of 1–2%. This oil matrix allows the lipophilic compounds found in chasteberry leaves to function effectively. extracting it and increasing its potential for penetration into the scalp This provides the total oil-based extract obtained as a result of this application. The phenolic compound content is approximately in the range of 80–200 mg / L, and the total flavonoid content is... It is standardized within the range of 60–150 mg / L. In another method, apple cider vinegar is used as a solvent and for extraction. The medium is standardized to a pH range of 2.5–3.5, preferably approximately pH 3. This acidic phase is particularly suitable for high concentrations of hydrophilic phenolic and flavonoid compounds. It supports the efficient transition to the solvent phase. The vinegar-based solution obtained by this method... The total phenolic content of the extract is approximately in the range of 1700–2200 mg / L. The total flavonoid content will be standardized to be in the range of 650–900 mg / L. is being done. In both methods, the extracts obtained are analyzed using analytical methods (e.g. Spectrophotometric determination of total phenolic and flavonoid content) is used to characterize and They are standardized within the specified ranges. This allows for batch-based standardization. Variation is minimized, repeatable quality is ensured, and biological A predictable profile is obtained in terms of activity. This holistic approach has been developed. Thanks to the extraction approach, both lipophilic and hydrophilic properties of chaste tree leaves are preserved. The active ingredients are being evaluated in a controlled manner; hair follicle cell viability a plant-based active ingredient that supports, has high antioxidant capacity and enhanced stability. The system is obtained. Thanks to these controlled parameters, chaste tree leaves are both lipophilic and hydrophilic. The active ingredients are obtained with high efficiency, minimizing oxidative degradation. and batch-based variation is limited. The scope of protection of the invention is set out in the attached claims and is strictly adhered to. The detailed explanation cannot be limited to the examples given. Because in technology... A specialist, without deviating from the main theme of the invention, will do what is described above. It is clear that similar structures can emerge in light of this. 6

Claims

1. Obtaining an extract from plant material to prevent hair loss. towards - increasing the contact surface area between the target phytochemical compounds and the solvent phase to initiate the transfer of plant material to the environment with the solvent mixing, - by weakening the cell wall integrity through cavitation effect, phenolic and to increase the extraction yield of flavonoid compounds in the mixture ultrasonic processing, - by suppressing oxidative enzyme activity, it prevents the degradation of active compounds. to reduce and increase extract stability during extraction Timed light application, - to ensure the obtainment of a phase enriched with active ingredients separation of the extract at the end of the process, A production method for a formulation containing a chasteberry leaf extract, including the following steps. Its characteristic feature is that the plant material is Vitex agnus-castus L. (chaste tree) leaves. and the extract of the leaves of Vitex agnus-castus L. (chaste tree) in question the following to be done b. Solvent-biomass contact of Vitex agnus-castus L. (chaste tree) leaves To increase the diffusion rate by optimizing the ratio 1:4–1:6 (w / v) mixing with solvent in a certain ratio, b. To increase cell wall permeability by creating controlled cavitation and to maximize active compound release in the 18–22 kHz frequency range and subjected to ultrasonic treatment in the power range of 350–450 W, c. to prevent thermal degradation of heat-sensitive phytochemical compounds processing at a temperature range of 25–30 °C for a period of 30–60 minutes e. to suppress oxidative enzyme activity and phenolic and flavonoid compounds To increase stability, a wavelength of 430–470 nm is used during extraction. blue light application in the height range It includes the steps of the process. 7.

2. Production method of a formulation containing a chaste tree leaf extract according to Claim 1. and its characteristic is that it is obtained by cold pressing method as a solvent medium in step a. The method involves using processed pumpkin seed oil.

3. Production method of a formulation containing a chaste tree leaf extract according to Claim 1. Its distinguishing feature is that apple cider vinegar is used as the solvent in step A.

4. Production method of a formulation containing a chaste tree leaf extract according to Claim 2. and its characteristic is that the fatty acid composition of the pumpkin seed oil in question palmitic acid 10–16%, stearic acid 3–7%, oleic acid 18–38%, linoleic acid 40–62% and linolenic acid at most 1–2% is selected.

5. Production method of a formulation containing a chaste tree leaf extract according to Claim 3. Its characteristic feature is that apple cider vinegar is used as a solvent. The pH value should be between 2.5 and 3.

5. 8