Multiparameter In Vitro / Ex Vivo Method and System for Measuring and Classifying the Differential Response to Substance-Specific Electromagnetic Stimulation in Hair Keratin Matrix

Abstract

The invention encompasses a multiparameter method and system for the molecular and biophysical characterization of human or mammalian hair samples under ex vivo / in vitro conditions. The method measures the basal Raman / FTIR spectral profile, basal QCM-D acoustic / mass profile, and basal BIS dielectric profile of the hair keratin matrix. Subsequently, a substance-specific THz / EM stimulation frequency belonging to the target substance or substance category is applied to the analysis cell, and the post-stimulation profile is recorded. The differential deviation parameters between the basal profile and the post-stimulation profile are calculated. These deviations are normalized with standard deviation values ​​obtained from blank sensor measurements, negative control matrices, and unstimulated control samples to calculate the Differential Response Index. The system output is not a medical diagnosis but a technical characterization classification. The invention offers multi-sensor fusion and control-based normalization above single-parameter analysis methods by enabling the measurement of differential physical response to substance-specific stimulation on the hair keratin matrix.