COMPOSITION AND PRODUCTION METHOD OF A DERMOCOSMETIC CREAM CONTAINING PLANT EXOSOMES FOR MUSCLE AND JOINT COMFORT.

Abstract

The invention relates to a dermocosmetic cream composition containing plant exosomes, developed to provide relaxation, increased comfort, and skin care support in muscle and joint areas, and the method of producing this composition.

Description

PLANT EXOSOME-BASED FOR MUSCLE AND JOINT COMFORT Dermocosmetic Cream Composition and Production Method TECHNICAL FIELD The invention provides relaxation, increased comfort, and skin care support in muscle and joint areas. a dermocosmetic cream containing plant exosomes developed to provide It is related to the composition and the method of producing this composition. PREVIOUS TECHNIQUE Used to provide relaxation and comfort in muscle and joint areas. Topical products are generally pharmaceutical gels containing synthetic active ingredients, or those with herbal ingredients. soothing gels and dermocosmetic products based on exosome technology They can be evaluated in three groups. Topical products containing synthetic active ingredients include non-steroidal antisteroids such as diclofenac. Anti-inflammatory agents are used. Products like Voltaren or Voltarol, In the form of a pharmaceutical gel applied to the skin to reduce pain and inflammation. These products are offered. While providing effective solutions, they contain synthetic ingredients. It is based on. Herbal topical soothing products contain menthol, camphor, and vegetable oils. Various plant extracts are used. Orzax Cosakondrin Gel contains hyaluronic acid. Boswellia serrata, ginger oil, eucalyptus oil, lavender oil, clove oil, arnica and It is presented as a relaxing gel containing menthol. Nutraxin Extra Gel and similar products. The products also belong to the same product group and mostly provide temporary coolness when used with massage. and provides relaxation. The active ingredients found in classic herbal gels and ointments are often free-form. It is included in the form. This allows the active ingredients to cross the skin barrier and be applied. This limits the ability of plant-based raw materials to reach sufficient density in the region. The products also vary depending on seasonal, geographical and production conditions. This can lead to variations in content and quality. Therefore, natural ingredients are preferred. Ensuring standardization and repeatable production of topical products. It is becoming more difficult. Current dermocosmetic products and studies using exosome technology. its main focus is skin regeneration, anti-aging care, and skin barrier repair. 1 It focuses on moisturizing and improving aesthetic appearance. This Exosomes in products generally support skin cell regeneration, To reduce skin sensitivity caused by inflammation or to improve the appearance of the skin. It is used for this purpose. Therefore, existing exosome-based dermocosmetics Solutions are mostly focused on the facial area and aesthetic skin care, muscles and joints. They are not being developed with the aim of providing local relief and comfort in these regions. The use of okra, ginger, and green tea in topical compositions. It has various supporting properties. Okra is rich in polysaccharides and mucilage. Thanks to its rich composition, it provides moisture balance, biocompatibility, and soothing effects. It constitutes a suitable plant-based source. Okra-derived components It is also reported to have anti-inflammatory and analgesic potential. Ginger contains components such as 6-gingerol and 6-shogaol, which are ginger-derived exosomes. a plant that has attracted attention due to the anti-inflammatory effects of similar nanoparticles Green tea is a source of antioxidants, particularly rich in catechins, especially EGCG. and exhibits anti-inflammatory properties. Green tea, when applied topically to the skin, helps to improve skin health. supporting protection against environmental stress and suppressing the inflammatory response It has potential. However, in the previous technique, okra, ginger and green tea were used. exosomes obtained from plants under controlled tissue culture conditions in muscle and joint to provide relief and comfort in these areas, with the same topical cream composition. There is no structure in which it is brought together. THE PURPOSE OF THE INVENTION The invention has applications in biotechnology, dermocosmetics, pharmaceutical excipients and topical products. It falls under the category of skincare products and provides relief and comfort to muscles and joints. a plant-based exosome-containing product developed to increase skin growth and provide skin care support This relates to the composition of dermocosmetic creams and the method of producing this composition. The invention includes okra (Abelmoschus esculentus), ginger (Zingiber officinale) and green Obtained from tea (Camellia sinensis) plants by tissue culture and isolation methods. The extracted exosomes are placed in a cream formulation suitable for topical application. They are brought together. These exosomes, which are nano-sized natural vesicles, are active biological molecules that support the transport of biomolecules from the skin surface to the application area They are used as carriers. In this respect, the invention is relevant to plant tissue culture and exosomes. Isolation, exosome characterization, and cream formulation stages are all carried out in the same production process. 2 an approach that combines natural ingredients, biocompatibility, and standardization. It relates to topical dermocosmetic product technology. The primary aim of the invention is to provide local relief and comfort in muscle and joint areas. to provide exosomes derived from okra, ginger, and green tea in the same cream matrix The goal is to develop a dermocosmetic composition containing plant-based ingredients. For this purpose, okra (Abelmoschus esculentus) and ginger (Zingiber) are used. (Camellia officinale) and green tea (Camellia sinensis) plants were cultured under controlled tissue culture conditions. They are being propagated, and exosome-containing active phases are isolated separately from each plant, and The obtained active phases are characterized and standardized. Standardization The provided exosome-containing active phases are then a topical application with increased bioavailability. They are brought together within a cream matrix. The invention uses okra-derived exosomes to restore the skin's moisture balance and Supporting biocompatibility, a soothing and relaxing effect at the application site. The aim is to contribute to the creation of the effect. Okra exosomes also a naturally derived supporting active ingredient in the formulation It is used as such. Exosomes derived from ginger combine with ginger's soothing and refreshing properties. The aim is to transport this active phase within a nano-vesicular structure. With its potential for anti-inflammatory and antioxidant effects, it is applied locally to muscle and joint areas. It is intended to contribute to increased comfort. Exosomes, catechins, and other antioxidant components derived from green tea. through which the skin's protection against environmental stress is supported, on the skin's surface Contributing to maintenance and repair processes and adding antioxidant capacity to the composition. The aim is to achieve this. With a plant tissue culture-based production approach, seasonal, geographical and raw material creating a controlled production process that is less affected by variations caused by external factors The aim is to prevent inter-batch content issues that can be seen in classic herbal products. reducing variability and achieving standardized, repeatable, and scalable production. The aim is to create the necessary infrastructure. Thanks to the nano-sized structure of exosomes and the carrier properties of the cream matrix transport of active ingredients across the skin barrier to the application area The aim is to facilitate this. With this structure, it is suitable for topical application and controlled. Obtaining a release-providing composition involves the use of active ingredients in classic herbal gels and... 3 Supporting more effective concentration in the target area compared to ointments. is the goal. Another aim of the invention is to offer an alternative to topical products based on synthetic ingredients. The aim is to offer a completely plant-based and biocompatible usage option. The composition... It is gentle because it does not contain parabens, SLS, steroids, and similar irritating ingredients. The aim is to increase its suitability for use on different skin types. The product, formulated as a cream, is easy to apply to the skin, absorbs quickly, and It is intended to be suitable for daily use. However, the developed production The approach is not limited to cream form only, the same technical principle can be applied to serum, gel, different topical product forms such as spray, roll-on or transdermal patch The aim is to make it applicable. DETAILED DESCRIPTION OF THE INVENTION The invention concerns a dermocosmetic cream composition for use on muscle and joint areas. It is designed to provide relaxation, increased local comfort, and skin care support. The composition includes an active phase containing okra exosome, and an active phase containing ginger exosome. active phase containing green tea exosome, topical cream base matrix, emulsifier system, moisturizing and / or carrier auxiliary phase, stabilizer and / or preservative system and pH It includes a balancing system. The active phase, containing okra exosome, constitutes 2-8% by weight of the composition. It is present in a proportion, preferably 4% by weight. Contains ginger exosome. The active phase is used at a concentration of 2-8% by weight, preferably 4% by weight. Green The active phase containing tea exosome should be 2-8% by weight, preferably 4% by weight. It is included in the composition in proportion to the percentage of the topical cream base matrix. It constitutes 65-88% by weight and is preferably used at 78% by weight. Emulsifier system at a concentration of 2-8% by weight, preferably 4% by weight. It contains a moisturizing or carrier auxiliary phase at a rate of 1-6% by weight. It is preferably used at a rate of 3% by weight. Stabilizer or protective system. It is present in amounts of 0.5-4% by weight, preferably 2% by weight. pH The stabilizing system should be 0.2-2% by weight, preferably 1% by weight. is included in the composition. For example, 100 g of the composition that is the subject of the invention The application included: an active phase containing 4 g (4%) okra exosome, and 4 g (4%) ginger. Active phase containing exosome, 4 g (4%); Active phase containing green tea exosome, 78 g (78%) Topical cream base matrix, 4 g (4%) emulsifier system, 3 g (3%) moisturizer and / or 4 carrier auxiliary phase, 2 g (2%) stabilizer and / or preservative system and 1 g (1%) pH A balancing system can be used. In the example formulation in question, the sum of the components... It is 100% by weight. The invention's production process involves the controlled propagation of plant materials. It begins. The active phase containing okra exosome, the active phase containing ginger exosome the plant to be used in obtaining the phase and the active phase containing green tea exosome The materials are subjected to tissue culture separately. Plant cultures are preferably stored at 22-28 °C. It is grown at a temperature, preferably 24-26 °C. 16 hours in the culture medium. A photoperiod consisting of light and 8 hours of darkness is applied, and the production is sterile. This is carried out under certain conditions. Obtaining a suitable amount of biomass from plants. Then, plant materials are collected and the exosome isolation stage begins. The harvested okra, ginger, and green tea materials are transported in a cold chain. It is processed while being preserved. Each plant source is homogenized separately and Supernatants containing exosomes are obtained. Low temperature during isolation. The conditions are maintained, the temperature preferably between 2-8 °C, more preferably around 4 °C. is maintained at this level. The coarse structures obtained as a result of homogenization particles by stepwise centrifugation and / or membrane filtration processes is removed. Following this process, the active phase containing okra exosome, ginger The active phase containing the exosome and the active phase containing the green tea exosome are separated from each other. They are purified. The exosome isolation process takes 1-8 hours, depending on the method used. The isolation and purification process is completed, preferably within 2-4 hours. The resulting exosomal suspensions are prepared under sterile conditions and For short-term storage, it is preferably kept at a temperature of 2-8 °C. Characterization of the obtained active phases before addition to the cream matrix. and standardization processes are carried out. Particle size is preferably dynamic. It is measured using the light scattering method, in other words DLS. Exosomes Morphological structure preferably examined using scanning electron microscopy and / or atomic force. using a microscope, in other words, through SEM and / or AFM analyses. It is being investigated. Biomarker verification is preferably done via Western blot analysis, while active ingredient profiling is performed using other methods. preferably by high-performance liquid chromatography, in other words HPLC analysis. is being evaluated. During the characterization phase, the particle size of exosomes is considered. preferably in the 30-150 nm range, or even better, around 80-120 nm. is the goal. Batches that do not meet the characterization and standardization criteria will not be produced. It is extracted from the process. The desired particle size, morphological structure, and biomarker. Batches showing the characteristics and active ingredient profile of the cream formulation It is being transferred to the next stage where it will be prepared. Apart from the preparation of exosome-containing active phases, topical cream base The matrix is ​​produced. In the preparation of the cream base, an emulsifier system and a moisturizer are used. or carrier auxiliary phase, stabilizer or preservative system and pH balancing system It is used. The oil and water phases of the cream base are prepared in separate containers. Both phases... It is preferably heated to a temperature of 60-75 °C, or even more preferably to 65-70 °C. The oil and water phases, having reached the desired temperature, are brought together under homogenization. An emulsion is formed. The homogenization process preferably takes 5-30 minutes, then... It is preferably continued for 10-20 minutes. Obtaining a homogeneous emulsion... then a topical cream base matrix, preserving the structural integrity of exosomes. For this purpose, it is cooled in a controlled manner. The temperature of the cream base is preferably 20-35 °C. The active phase, preferably containing okra exosome, reaches a temperature of 25-30 °C. Active phase containing ginger exosome and active phase cream base containing green tea exosome They are added into the matrix. The active phases can be added to the cream base sequentially, or... They can also be added together. To prevent the degradation of the exosome structure during the addition of active phases. For this purpose, low-shear mixing is applied. The mixing process is preferably done in 5-20 seconds. The process is continued for minutes, preferably 8-15 minutes. Within the formulation If air bubbles are found, vacuum may be applied as needed. Deaeration treatment can be applied. Exosome-containing active phases homogeneously distributed within the cream matrix. After dispensing, the pH value of the final product is adjusted using a pH balancing system. is being adjusted. The pH value of the final formulation is preferably in the range of 4.8-6.0, or higher. Ideally, the pH range is adjusted to 5.0-5.8. This pH range allows the formulation to be suitable for the skin. The aim is to increase harmony. The completed cream composition was tested for homogeneity, viscosity, and phase separation. They are checked for microbiological suitability and stability. Stability tests Observations are preferably carried out under temperature conditions of 4 °C, 25 °C and 40 °C. The duration is preferably set at 1-3 months, with tests preferably taking place every 3 months or longer. The duration is being continued. 6 Products found to be suitable as a result of the inspections include tubes that reduce contact with air. The product is filled into airless pumps or similar suitable packaging. It is stored protected from high temperatures, preferably within a temperature range of 15-25 °C. Apply the product to clean, dry skin, muscle, or joint area during use. It is applied in sufficient quantity. The cream composition is applied lightly to the application area. It is spread by massage. Avoid direct contact of the product with eyes and open wounds. It is preferred that it not be consumed. The composition can be consumed daily depending on its intended use. It can be applied 1-3 times, preferably twice a day. The active phase, containing okra exosome, is a plant-based compound rich in polysaccharides and mucilage. Due to its source, it contributes to supporting the skin's moisture balance and biocompatibility. This active phase also provides a soothing effect at the application site. It is added to the composition to create a relaxing effect. The active phase, containing ginger exosome, provides ginger's soothing and refreshing properties. This enables the transport of its properties within nano-vesicular structures. This active phase With its potential for anti-inflammatory and antioxidant effects, it is applied locally to muscle and joint areas. It is intended to contribute to increased comfort. The active phase contains green tea exosome, catechins, and antioxidant components. Through green tea, it helps protect the skin against environmental stress. The derived active phase also contributes to the care and repair processes on the skin's surface. It is present and enhances the antioxidant capacity of the formulation. All three active phases were obtained under controlled conditions via plant tissue culture. This helps to reduce variability caused by seasonal and geographical differences. This approach also provides opportunities for the relationship between plant-based raw materials. limiting the impact of differences on the production process and creating more consistency between parties. It enables the acquisition of content. The nano-sized vesicular structure of exosomes allows active biomolecules to be released into the skin. It supports passage through the barrier and transport to the application area. Active Suitable for topical application due to the use of phases within the cream matrix. A composition that provides controlled release is obtained. The fact that the composition is prepared in cream form allows the product to be easily applied to the skin surface. its application, rapid absorption, and suitability for daily use The formulation is free of parabens, SLS, steroids, and similar irritating ingredients. It does not include. 7

Claims

1. Provides relief in muscle and joint areas, increases local comfort, and supports skin care. a dermocosmetic cream applied topically to provide Its composition is characterized by its active phase containing okra exosome and ginger. Exosome-containing active phase, green tea exosome-containing active phase, topical cream base matrix, emulsifier, humectant and / or carrier auxiliary phase, stabilizer and / or are characterized by containing preservatives and pH balancers.

2. According to claim 1, it is a dermocosmetic cream composition with the characteristic of being; 2% by weight. The active phase contains 8% okra exosome and 2-8% ginger by weight. The active phase containing the exosome contains 2-8% green tea exosome by weight. active phase, 65-88% by weight; topical cream base matrix, 2-8% by weight. emulsifier at a certain percentage, 1-6% by weight of humectant and / or carrier Auxiliary phase, 0.5-4% by weight of stabilizer and / or preservative and It is characterized by containing 0.2-2% pH stabilizer by weight.

3. According to claim 2, it is a dermocosmetic cream composition with the characteristic of having 4% by weight. Active phase containing okra exosome at a certain percentage, ginger at 4% by weight. The active phase containing the exosome contains 4% green tea exosome by weight. Active phase, 78% by weight; topical cream base matrix, 4% by weight. emulsifier at a certain percentage, 3% by weight of humectant and / or carrier Auxiliary phase, 2% by weight stabilizer and / or preservative and by weight It is characterized by containing 1% pH balancer.

4. According to claim 1, it is a dermocosmetic cream composition with the following characteristic: okra The active phase containing the exosome is found in okra (Abelmoschus esculentus) and ginger. active phase containing exosome of ginger (Zingiber officinale) and green tea exosome containing the active phase obtained from green tea (Camellia sinensis) plant material It is characterized by containing exosomes.

5. According to claim 1, it is a dermocosmetic cream composition with the following characteristic: okra active phase containing exosome, active phase containing ginger exosome, and green tea The exosomes in the active phase containing the exosome are 30-150 nm particles. It is characterized by its size.

6. According to claim 5, it is a dermocosmetic cream composition, and its characteristic is; Exosomes are characterized by having a particle size of 80-120 nm. 8 7. According to Claim 1, it is a dermocosmetic cream composition, characterized by its pH value. It is characterized by being in the range of 4.8-6.

0.

8. According to claim 7, it is a dermocosmetic cream composition, characterized by its pH value. It is characterized by being in the range of 5.0-5.

8. 9