CHESTNUT SHELL NANO-PARTICLE BASED, MOISTURE AND PH SENSITIVE, DOUBLE-LAYER PDO NANOFIBER ANTIFUNGAL WOUND DRESSING PROVIDING CONTROLLED TANNINE RELEASE.

Abstract

The invention relates to an antifungal wound dressing with a double-layered nanofiber structure containing chestnut shell nanoparticles, formed by electrospinning, consisting of a PDO-based mechanical carrier substrate in the lower layer and a pH-sensitive polymer phase in which chestnut shell nanoparticles are dispersed in the upper layer.

Description

CHESTNUT SHELL NANO-PARTICLE BASED, MOISTURE AND PH SENSITIVE. DOUBLE-LAYER PDO NANOFIBER PROVIDING CONTROLLED TANNINE RELEASE. ANTIFUNGAL WOUND DRESSING TECHNICAL FIELD The invention involves nanoparticles of chestnut shell (Castanea sativa) and It has a double-layered nanofiber structure created by electrospinning, with a lower layer It is a mechanical carrier structure based on polydioxanone (PDO) and has a top layer of chestnut shell. Antifungal wound dressing consisting of a pH-sensitive polymer phase with dispersed nanoparticles. It is related to. PREVIOUS TECHNIQUE Nanofiber wound dressings used in wound care are mostly produced using electrospinning. It is produced using this method. In current applications, polycaprolactone (PCL) is primarily used. A suitable biodegradable or synthetic polymer such as polylactic acid (PLA) or PDO. It is dissolved in a solvent. It has antifungal or antibacterial properties. Synthetic drugs or herbal extracts used for the purpose of acquiring them, prepared It is added directly to the polymer solution. The mixture is then homogenized. The nanofiber structure is being loaded into the electrospinning device under high voltage. It is formed and the resulting product is dried and prepared as a wound dressing. In wound dressings produced in this way, the active ingredient is mostly contained within a polymer matrix. It is randomly distributed. The active substance is added directly to the polymer solution. Therefore, there must be a functional gap between the release zone and the mechanical carrier zone. A distinction cannot be made. In the literature, nanofiber wound healing containing synthetic antifungal agents is discussed. coatings, plant extract-loaded electrospun membranes and single-layer nanofibers Although wound dressing systems have been widely described, most of these structures... a layered architecture or controlled release that operates according to the conditions of the wound environment. There is no mechanism. The active substance is directly incorporated into the polymer matrix. mixing, after the wound dressing comes into contact with a moist environment, the active substance This can lead to a significant portion of them being released in a short time. Fast This phenomenon, referred to as initial release, involves the release of the active substance over time. This makes it difficult to release the product in a balanced manner. High levels in the initial stages of release. 1 While a certain amount of active substance is released at first, the amount released may decrease in subsequent stages. And maintaining a long-term antifungal effect can become difficult. Another problem encountered in current systems is related to wound exudate. the controlled effect of the resulting increase in humidity on the release behavior The problem is that it cannot be used. The polymer structure swells uncontrollably in a humid environment. This can happen, the resulting diffusion pathways cannot be regulated, and consequently... The release rate of the active ingredient cannot be adjusted as desired. pH changes that may occur in the wound environment are also present in the nanofiber wound. pH is not considered a release-regulating factor in most of the coatings. a structure that changes its permeability or swelling behavior depending on the change When absent, the release rate of the active substance depends on whether the wound environment is acidic or basic. It occurs regardless of the conditions. Therefore, the release mechanism... It is unable to adapt to the changes occurring in the wound environment. In systems where plant extracts are directly added to the polymer solution, the active the components are also affected by thermal or chemical conditions in the production process This is possible. This situation can lead to the degradation of plant-derived active ingredients and This can lead to a decrease in their stability, especially of tannins and phenolic compounds. stable preservation within the structure and controlled release in the wound environment This abandonment cannot be achieved to an adequate degree in the current systems. Single-layer nanofiber structures provide mechanical strength and active ingredient. It attempts to perform emission regulation functions within the same structure. As a result, mechanical transport and controlled release characteristics differ. It is becoming difficult to regulate it independently. Aimed at increasing mechanical strength. Configuration can influence release behavior, aiming to facilitate release. However, this configuration negatively affects the structural integrity and mechanical stability of the wound dressing. It can have an effect in that direction. In the literature, only bilayer electrospun structures that are sensitive to ambient pH have been reported. these systems are defined and predominantly use synthetic drugs as active ingredients. It is used. However, increase in humidity is considered as an independent stimulus. evaluating and developing a naturally derived bioactive compound in nanoparticle form. No approach that ensures controlled release while protecting against contamination has been found. Furthermore, the existing A dual-trigger release system that utilizes humidity and pH changes simultaneously in the systems. It lacks a mechanism; it features layered architecture with mechanical support and controlled release. 2 Their functions are not separated from each other and stable nanoparticles of natural tannins It cannot be released in a controlled, targeted manner while being preserved in its original form. THE PURPOSE OF THE INVENTION The aim of the invention is to provide a natural antifungal agent in a moist and pH-variable wound environment. the controlled, environmentally sensitive and time-spread release of the agent The goal is to develop a biodegradable nanofiber wound dressing that provides this. Another aim of the invention is to analyze the tannins and phenolic compounds found in chestnut shells. By incorporating the components in nano-particle form into the wound dressing structure, the aforementioned active ingredients... The aim is to ensure the stable preservation of the components. Cryogenic chestnut shell. By reducing them to nano-particle size through a grinding method, during grinding... to prevent possible thermal decomposition and preserve the active ingredient that is intended. The invention also leverages the large surface area provided by nanoscale chestnut shell particles. By taking advantage of the area and homogeneous distribution within the structure, tannin release is improved. It aims to achieve this in a balanced manner. Nano-particles on the top layer rapid start by homogeneous distribution at a specified loading rate reducing release and extending the antifungal effect over a longer period of time. is the goal. One of the aims of the invention is to improve the mechanical support function of the wound dressing. The goal is to distribute the controlled release function across different layers. In this context, PDO-based sub-layers... The layer acts as a mechanical and biodegradable carrier, pH-sensitive polymer phase. The top layer contains the active ingredient and regulates its controlled release. The aim is to differentiate between mechanical strength and functional release characteristics. The aim is to enable independent regulation. Another aim of the invention is to counteract the increase in moisture associated with wound exudate by promoting tannin release. The goal is to use it as a trigger that initiates the process. The top layer absorbs water in a humid environment. by causing it to swell in a controlled manner, and as a result of this swelling, allowing the grain to pass through. The aim is to create diffusion channels. However, the invention involves the upper layer. The pH-sensitive polymer phase present depends on the acidic or basic conditions of the wound environment. by changing its permeability and thus the tannin release rate according to the pH of the environment. It aims to regulate this. This is due to the combined effect of humidity and pH changes. Thanks to its design, it provides a controlled release with a dual trigger that is sensitive to environmental conditions. The aim is to obtain the mechanism. 3 DETAILED DESCRIPTION OF THE INVENTION The invention concerns an antifungal wound dressing containing chestnut shell nanoparticles. It has a double-layered nanofiber structure created by the electrospinning method. The lower layer of the wound dressing consists of a PDO-based mechanical carrier structure, and the upper layer is... It consists of a pH-sensitive polymer phase in which chestnut shell nanoparticles are dispersed. It is coming. Chestnut shell nanoparticles are a natural antifungal active ingredient in wound dressings. It forms the source of the tannins and phenolic compounds found in chestnut shells. It provides an antifungal effect, and its nano-particle structure allows these components to be applied to the wound. It allows for the controlled transfer of the virus to its environment. Chestnut shells undergo cryogenic grinding for the preparation of nanoparticles. It is subjected to. During grinding carried out under cryogenic conditions, the temperature... The increase is limited, thus preventing the tannins and phenolic compounds found in chestnut shells from increasing. Thermal degradation of the components is prevented. As a result of the grinding process, 500 to Stable chestnut shell nanoparticles in the 1000 nm range are obtained. Reducing chestnut shells to nano-particle size increases the surface area of ​​the particles. increasing and more homogeneously forming the polymer phase within the top layer. This structure allows for the distribution of the active ingredient in specific areas. limiting concentration and ensuring more balanced tannin release throughout the structure. It contributes to the realization of this in this way. The underlayer of the wound dressing consists of PDO (polydioxanone). It acts as a biodegradable mechanical carrier and has a double-layered nanofiber structure. It ensures structural integrity. PDO solution is used to form the substrate. is being prepared and the PDO will reach a viscosity suitable for the electrospinning process. It is being dissolved. The prepared PDO solution is subjected to electrospinning and nanofibers are formed. It is accumulated in this form to create a mechanical carrier substrate. This substrate, It provides mechanical strength to the wound dressing and the active top layer to be placed on it. It creates a biodegradable carrier surface for the layer. The top layer is a pH-sensitive polymer phase with chestnut dispersed within this phase. Its shell consists of nanoparticles. After the pH-sensitive polymer phase is prepared... then, chestnut shell nanoparticles obtained by cryogenic grinding method It is added to the phase in question at the specified loading rate. Nano- 4 The particles are dispersed in such a way as to obtain a homogeneous distribution within the upper phase. is being done. Regulating the nanoparticle loading rate increases the onset rate of tannin release. and affects its time-dependent distribution. The loading rate must be controlled. With its identification, the active ingredient is highly effective immediately upon contact with the wound environment. the amount of tannin released is reduced and the release of tannins is prolonged. It is ensured that it spreads across the range. The upper phase, containing chestnut shell nanoparticles, was produced by electrospinning. The data is deposited onto a pre-existing PDO substrate. As a result of this process... from a mechanical carrier substrate and an active top layer that provides controlled release A double-layered nanofiber structure is obtained. In a double-layered structure, the functions of the layers are separate from each other. The PDO substrate provides mechanical strength, biodegradability, and transport capacity, while the top layer... The layer contains tannins and phenolic compounds and their release into the wound environment. It regulates the structure and active substance necessary for mechanical transport. The structure required for its release is not brought together within the same layer; both The function is performed through its own layer. When a wound dressing is exposed to the moist wound environment, the pH level in the upper layer changes. The sensitive polymer phase absorbs water from the wound exudate. Water absorption. As a result, the top layer swells in a controlled manner, and tannins form within the polymer structure. Diffusion channels are formed that allow the passage of phenolic compounds. These channels... Upon formation, tannin release begins from chestnut shell nanoparticles. Tannin release is not solely dependent on the effect of humidity. (Top) The pH-sensitive polymer phase in the layer depends on the pH of the wound environment. It alters permeability and swelling behavior. The acidic or basic conditions of the environment... affecting the permeability of the top layer and, consequently, the nanoparticles The rate at which the released grain passes through the upper layer changes. Increased moisture causes the upper layer to swell and diffusion channels to form. While providing moisture, pH changes regulate the permeability of this layer. Thus, moisture and pH, moisture, and pH together drive tannin release; dual-triggered controlled tannin release. It functions as two separate environmental triggers that make up its mechanism. Environment The tannin release rate is modulated depending on changes occurring in the conditions. This ensures that the uncontrolled release of the active ingredient is limited. The wound dressing is produced through cryogenic grinding of chestnut bark. It begins. As a result of cryogenic grinding, chestnuts are processed in the range of 500 to 1000 nm. Shell nanoparticles are obtained. Following this, a PDO solution is used. PDO-based nanofiber substrates are being prepared and manufactured using the electrospinning method. is being created. After the substrate is prepared, a pH-sensitive polymer phase is formed and Chestnut shell nanoparticles are in this phase at the specified loading rate. It is homogeneously distributed. The resulting active top phase is applied to the PDO substrate. It is deposited by electrospinning method and is a double-layered nanofiber wound dressing. is being created. The resulting wound dressing is exposed to a moist wound environment with varying pH values. When left undisturbed, the top layer swells by absorbing water, depending on the pH value. altering its permeability and with tannin found in chestnut shell nanoparticles It releases phenolic compounds in a controlled manner. The nano-particle structure is dual. Layered architecture and a moisture and pH-sensitive top layer work together for a quick start. reducing its release, spreading tannin release over time, and enhancing the antifungal effect. It ensures its continuation. 6

Claims

1. In a moist and pH-variable wound environment, the natural antifungal active ingredient... a method used to ensure controlled and time-spread release It is an antifungal wound dressing, characterized by its double-layered structure created using the electrospinning method. a layered nanofiber structure, the mechanical properties of the double-layered nanofiber structure in question a polydioxanone-based substrate that provides the carrier and the substrate It swells in the moist wound environment and adapts to the pH of the wound environment. by changing its permeability depending on the chestnut shell nanoparticles Chestnut shell nano- that enables the controlled release of the contained grain. an upper layer consisting of a pH-sensitive polymer phase in which the particles are dispersed It is characterized by its inclusion.

2. Antifungal wound dressing according to Claim 1, consisting of chestnut bark nanoparticles. It is characterized by having a particle size range of 500 to 1000 nm.

3. An antifungal wound dressing according to claim 1 or 2, characterized by its composition: chestnut bark. by preventing the thermal decomposition of the tannins and phenolic compounds present. the stable protection of the components within nanoparticles for this purpose, reduced to nano-particle size by cryogenic grinding method Chestnut shells are characterized by containing nanoparticles.

4. In a moist and pH-variable wound environment, the natural antifungal active ingredient... a method used to ensure controlled and time-spread release It is a method of producing antifungal wound dressings, and its characteristic feature is that chestnut bark is used. Tannins with an average particle size of 500–1000 nm obtained by cryogenic grinding method. conversion of a PDO-based polymer solution into charged nanoparticles prepared and mechanical carrier substrate by electrospinning method formation, preparation of pH-sensitive polymer phase, resulting tannin-loaded homogeneous distribution of nanoparticles within the polymer phase in question and this phase is deposited onto the substrate using the electrospinning method, thus forming a double layer. including the process steps for obtaining a layered nanofiber structure It is characteristic. 7