OLIVE PIECE-DERIVED PYROLYGNOSIC ACID-BASED SOLUTION AND PRODUCTION METHOD

Abstract

The invention relates to the production of a multipurpose solution based on pyrrolignomous acid obtained by pyrolysis of olive pits, and its characteristics include the following process steps: pyrolysis of olive pits in an oxygen-free or oxygen-limited environment at a temperature between 400°C and 700°C; concentration of volatile components formed during pyrolysis and separation of the liquid fraction containing pyrrolignomous acid; settling of this liquid fraction; separation of the tar phase from the settled liquid fraction; filtration of the liquid fraction separated from the tar phase to obtain purified pyrrolignomous acid; mixing of the obtained purified pyrrolignomous acid with water; addition of at least one organic or mineral acid component to this mixture; and mixing of the components until a homogeneous solution is obtained.

Description

OLIVE PIECE-DERIVED PYROLYGNOSIC ACID-BASED SOLUTION AND PRODUCTION METHOD Technological Field: The invention is a multipurpose product based on pyrrolignoous acid obtained by the pyrolysis of olive pits. production of solutions and their application in food cleaning and agricultural environments. It relates to its use in regulatory applications. State of the Art: Water, vinegar, and baking soda are most commonly used to clean fruits and vegetables before consumption. or washing products with different chemical compositions are used. However, this applications, pesticide residues and microorganisms found on the product surface It is not always possible to ensure a simultaneous and sufficient reduction. Only In methods based on physical cleaning, the residues adhering to the surface are completely removed. inability to remove, and in the case of products containing strong chemicals, food contact safety, Issues such as residue formation and environmental sustainability may arise. The pH and electrical properties of water used in agricultural irrigation and hydroponic cultivation. Conductivity values ​​affect plant growth and nutrient availability. It has a direct effect. In current practices, pH regulation is mostly achieved through minerals. This is achieved using acids, while electrical conductivity is achieved through different processes or It is controlled through additional regulators. This situation affects multiple products. its use, the individual determination of application quantities and cultivation leading to a more complex management of the chemical balance in the environment is happening. In addition, there are products resulting from agricultural activities such as olive pits. A significant portion of biomass-derived by-products are wasted without generating sufficient added value. 1. It is either disposed of or used in limited areas. Obtained from this biomass. The pyrrolignoous acid obtained is subjected to appropriate purification processes and then processed in a controlled, homogeneous manner. and to transform it into a solution that can be adapted to different application areas. Solutions are limited. Therefore, both in food cleaning and agricultural environments... can be used in its regulation, using natural resources and a common production method. There is a need for multipurpose solutions that can be prepared. Description of the invention: The main purpose of the invention is to produce pyrrolignomous substances from the pyrolysis of olive pits. controlled purification of acid and a solution suitable for various applications. This process involves cleaning fruits and vegetables before consumption. Reducing pesticide residues and microbial load, as well as agricultural irrigation water and It is possible to regulate pH and electrical conductivity values ​​in hydroponic solutions. This results in existing solutions that are only intended for a single purpose. Instead, a multi-purpose product is obtained that can be produced with the same production infrastructure. The main component of the solution is derived from an agricultural byproduct such as olive pits, It enables the transformation of waste biomass into a value-added product. This situation reflects dependence on petrochemical or purely mineral acid-based products. a more sustainable and circular production approach while contributing to its reduction It constitutes. In applications for cleaning fruits and vegetables, the solution removes substances found on the product surface. It helps reduce pesticide residues while protecting against bacteria, viruses, and fungi. It also helps to reduce the microbial load. It provides both cleaning and antimicrobial effects. Bringing them together in the same solution eliminates the need to use separate products. This reduces the need for it. The products can also be rinsed with water after application. It provides ease of use. The invention can be used in agricultural applications to provide irrigation water and hydroponic nutrients. The pH of the solution or growing medium can be controlled and regulated. 2In addition, it contributes to the adjustment of the electrical conductivity value, high reducing the negative effects of salinity conditions on the plant and providing nutrients It helps the plant absorb the elements under more favorable conditions. The solution quantity can be gradually adjusted according to the measured values, suitable for different plants. It offers flexible usage depending on the species and growing conditions. The same basic solution at different concentrations and with different auxiliary components. Its applicability is a significant advantage in terms of production and product development. It constitutes. Suitable for food contact in food cleaning applications. While various components can be used, biochar, humic acid, and fulvic acid are also used in agricultural applications. the substance consists of auxiliary components such as seaweed extract or amino acids. It can be used. The invention also provides flexibility in terms of application methods. Solution for fruit. and can be applied to vegetables by soaking, spraying or washing; agricultural in use directly into irrigation water, hydroponic solution or growing medium Additional options are available. This allows the same product to be used in different business sizes and at different times. It can be evaluated with application equipment and under different usage conditions. Detailed Description of the Invention: The terminology used here is intended solely to describe specific applications. and does not limit the scope of the invention. Used here The term "and / or" refers to any one or more of the items listed as related. It includes one and all combinations thereof. Also, the singular "one" used here, "one" The terms "number" and "specified" are used in their plural forms unless the context explicitly indicates otherwise. It is designed to include singular forms such as those mentioned above. Furthermore, the terms used in this specification... The terms "includes" and / or "contains" refer to the specified features, steps, processes, It indicates the presence of elements and / or components, but one or more other features, steps, processes, elements, components and / or groups thereof It will be understood that this does not exclude its existence or addition. 3Unless otherwise stated, all terms used herein (including technical and scientific terms), in the sense that a person with general knowledge in the field to which this invention belongs would generally understand it They have the same meaning. Furthermore, as defined in commonly used dictionaries... The terms will have a meaning consistent with the context of the relevant field and this explanation. it should be interpreted as idealized unless otherwise explicitly defined here. or it will be understood that it will not be interpreted in an overly formal sense. The description of the invention will reveal a series of techniques and steps involved. These are: Each of them provides benefits individually, and at the same time, one or more of them can be used together. In some cases, all of the other techniques described may be used in combination. Accordingly, To ensure clarity, each step in the disclosure of the invention should be presented as accurately as possible. By doing this, unnecessary repetition of all combinations will be avoided. Together, the specification and claims, such combinations fully constitute an invention and claims. It should be read with the understanding that it falls within its scope. The invention utilizes pyrrolignoous acid obtained from the pyrolysis of olive pits. a multipurpose solution prepared and used in various application areas and words The subject matter relates to the production method of the solution. The solution that is the subject of the invention is essentially... It contains purified pyrrolignoous acid and water, and its composition depends on the area to be applied and the desired result. Depending on the desired effect, at least one organic or mineral acid in food or agriculture. It may include auxiliary components suitable for its applications. The pyrrolignoous acid used in this invention is obtained from olive pits in the absence of oxygen. or as a result of being subjected to pyrolysis in an environment where the amount of oxygen is limited Olive pits are obtained during agricultural production and food processing processes. It is a biomass-derived byproduct obtained in the production of pyrrolignoous acid within the scope of this invention. It is considered a raw material. The pyrolysis process involves heating olive pits to 400°C. This can be achieved by subjecting the product to heat treatment at a temperature between 700°C. In a preferred practice, the pyrolysis temperature is maintained between 450°C and 650°C. During pyrolysis, the thermal decomposition of olive pits results in solids, liquids, and gases. Products containing phases are formed. Volatile components are formed during the process. The liquid pyrolysis product is obtained by condensation, and the liquid contains pyrrolignoous acid. Fraction 4 is separated from other pyrolysis products. The liquid obtained as a result of pyrolysis. fraction allowed to settle before being used directly in solution preparation It is subjected to processing. During the settling process, the heavy tar present in the liquid... The components, suspended particles, and phases with different densities are distinct from each other. It separates. At this stage, the tar phase that accumulates at the bottom or top of the liquid becomes pyrrolignomous. It is removed from the liquid phase containing acid. After the separation of the tar phase... Filtration of the liquid fraction containing pyrrolignoous acid and further purification as needed. The following processes are applied. The filtration process removes solid particles found in the liquid. reducing sediments and residues that could negatively affect performance. This is because the filtration process can be carried out with a single filtration stage. like, in multiple stages by using filters with different pore sizes This can be accomplished through settling, phase separation, and filtration processes. as a result of its completion, the main active component of the solution is formed. Purified pyrrolignomous acid is obtained. Purified pyrrolignomous acid is organic. acids, phenolic compounds, and other water-soluble components formed during pyrolysis It may include. The solution that is the subject of this invention is prepared by first mixing a specified amount of water with another The water is transferred to the mixing tank. The water used is pure water. The water transferred to the mixing tank... Purified pyrolignous acid is added to it in a controlled manner. Pyrolignous After mixing acid and water, the desired pH value is obtained. Depending on the application area and the performance of the solution, at least one acid component. can be added to the mixture. The acid component in question is phosphoric acid, citric acid, or The solution may contain another organic or mineral acid suitable for its intended use. In order to maintain the homogeneity of the mixture during the addition of the acid component The mixing process is continued. The mixing process takes place at a temperature between 15°C and 35°C. This can be done at a certain temperature. In a preferred application, the mixing temperature is... It is kept between 20°C and 25°C, and the components are left for 10 to 20 minutes. They are mixed. The solution obtained at the end of the mixing process has a homogeneous structure. After reaching its destination, it can be subjected to further filtration. With the final filtration process... particles that may be incorporated into the solution during mixing and form a precipitate The components are removed. The prepared solution is then prepared according to its intended use. It is filled into 5 packages. High density is used in the packaging of the solution. Polyethylene or polyethylene terephthalate-based packaging can be used. In a sample application of the invention, mixing is performed to prepare one liter of solution. 700 mL of pure water is added to the tank. Olive pit pyrolysis product is then added to the pure water. 250 mL of pyrrolignoous acid, which has been processed and filtered to separate it from the tar phase, is added. Then, 50 mL of phosphoric acid is added to the mixture in a controlled manner. The result... The resulting mixture is stirred at a temperature between 20°C and 25°C for 10 to 20 minutes. It undergoes final filtration and packaging when necessary. The specified quantities. It describes a sample application of the invention, including the component ratios of the solution used. the area, the target pH value, the composition of pyrrolignoous acid and the medium to be applied It can be modified according to its properties. Pyrolignous acid, water and acid component The quantities can be varied in different ratios, provided that the basic structure of the solution is preserved. It can be determined. The solution described in this invention is intended for cleaning fruits and vegetables before consumption. It can be used. In this application, the solution is applied to the surfaces of fruits and vegetables. reducing pesticide residues and microorganisms such as bacteria, viruses or fungi It is applied to reduce the quantity. Cleaning fruits and vegetables. During this process, the solution is transferred to an application container, and the products to be cleaned are mixed with the solution. They are brought into contact. The contact process involves keeping the products immersed in the solution. by spraying the solution onto the product surface or by washing the products with the solution. This can be done. In a preferred application, fruits and vegetables are mixed with the solution. It is left inside for approximately five minutes. After the waiting period is complete... Then the fruits or vegetables are removed from the solution and rinsed with tap water or for consumption. The product is rinsed using another suitable clean water source. This rinsing process removes the excess water from the product. solution remaining on the surface and residues separated from the surface by the solution is being removed. The solution used for cleaning fruits and vegetables must be suitable for contact with food. Provided that it supports stability, homogeneity or application performance. Components can be added. These auxiliary components alter the basic active structure of the solution. 6. The resulting pyrrolignostic acid must be compatible with water and, if present, the acid component. is selected. The solution described in this invention can also be used to convert irrigation water used in agricultural production into hydroponic water. For the purpose of regulating the pH value of the nutrient solution or growing medium. It can be used. In this application, the solution is based on the initial pH of the application medium. the amount determined by taking into account the target pH value that is desired to be reached. It is added to irrigation water or hydroponic solutions. The solution is used in agriculture. Its use in applications involves adjusting the pH of irrigation water or nutrient solution. bringing its value to a suitable range in terms of plant cultivation This ensures that the nutrients present in the growing medium are supplied to the plant. supporting its uptake by and from high or low pH values The aim is to reduce the resulting negative consequences. The solution is used in agricultural applications to regulate electrical conductivity values. It can also be used for other purposes. Application amount; irrigation water, hydroponics the initial electrical conductivity value of the solution or growing medium, the target taking into account the electrical conductivity value and the characteristics of the cultivated plant It is determined. In agricultural applications, the dosage of the solution is 0.05% to 5% by volume. The application dose, measured pH, and electrical conductivity can be among the factors considered. Depending on the values, this range can be increased or decreased. Solution It can be added directly to the irrigation water, or it can be diluted in the main mixing tank before irrigation. or it can be fed into a hydroponic system. In the agricultural use of the solution, pH and electrical conductivity values ​​are determined during application. It is measured beforehand, and then the determined amount of solution is added. The mixture is homogenized and the values ​​are measured again. The target is... If the desired values ​​are not reached, continue adding the solution gradually. This allows the amount of solution to be adjusted according to the characteristics of the environment where it will be applied. It is possible to adjust it. 7 In solution variants or together with the solution for agricultural application. The applications carried out include biochar, activated carbon, humic acid, and fulvic acid. seaweed extract, amino acids, or natural buffering components can be used. The components in question can be included in the solution. such as, separately from the solution, the same irrigation water, hydroponic solution or growing solution. It can also be applied to the environment. In applications where biochar is used in conjunction with a solution, biochar is used in olive oil production. It can be obtained as a result of the pyrolysis of the nucleus or other biomass. Biochar can be applied to the growing medium in solid form and is pyrrolignoous acid based. The solution can then be introduced into the environment along with the irrigation water. Alternatively, Biochar can be transported to the application environment by being brought into contact with the solution beforehand. Humic and fulvic substances are used, along with seaweed extracts or amino acids. In applications, the amount of these components depends on the type of plant being grown and the growing medium. These components are determined according to their properties. These components are essential for the solution that is the subject of the invention. optional auxiliary components used especially in agricultural applications are components. The invention describes a solution that is obtained through the same basic production processes. however, usage concentration and auxiliary components vary depending on the application area. It can be differentiated. In fruit and vegetable cleaning practices, food is used. when considering contact suitability, irrigation water or hydroponic system the characteristics of the plant grown in the applications and the pH and electrical conductivity of the environment values ​​are taken as the basis. The solutions evaluated within the scope of the invention aim to reduce pesticide residues. various Laboratory analyses have been performed. The results obtained are exemplified below. This is explained in the form of applications. 8In the comparative study, one of the plum samples of the same type and characteristics showed no signs of any... One sample was set aside as a control sample without undergoing any treatment, while the other sample is the subject of the invention. The samples were analyzed after being treated with the solution. The following were detected in the samples: The amounts of acetamiprid, trifloxystrobin, and fluopiram are given in the table below. The amounts of these pesticides in the control sample were 0.110 mg / kg and 0.035 mg / kg, respectively. Measured as mg / kg and 0.100 mg / kg. Treated with the solution that is the subject of the invention. In the sample, these values ​​were 0.054 mg / kg, 0.013 mg / kg, and 0.048 mg / kg, respectively. It has been determined. The solution that is the subject of the invention. Pesticide examined: Reduction (%) compared to Control sample (mg / kg) after treatment. (mg / kg) Acetamiprid 0.110 0.054 50.9 Trifloxystrobin 0.035 0.013 62.9 Fluopiram 0.100 0.048 52.0 The results obtained were tested on a sample treated with the solution that is the subject of the invention. Compared to the sample, the amount of acetamiprid was 50.9 percent and the amount of trifloxystrobin was 50.9 percent. It shows that the amount of fluopiram decreased by 62.9% and 52.0%. The results show that the solution in question reduces different pesticide residues. This shows that it can be used. The subject of the invention is a solution-based liquid sample, under contaminated conditions and containing 80% product by volume. Subjected to bactericidal efficacy test with a five-minute contact time at the given concentration. The study was conducted using the TS EN 1276 method and involved four different bacteria. The type was examined. The pyrrolignoous acid-based sample tested here contains 9 percent by weight. It has a total acetic acid content. 9 Product Contact Logarithmic Test microorganism evaluation concentration duration decrease value Staphylococcus aureus 80% 5 minutes >5.19 Effective ATCC 6538 Pseudomonas aeruginosa 80% 5 minutes >5.17 Effective ATCC 15442 Enterococcus hirae ATCC 80% 5 minutes >5.22 Effective 10541 Escherichia coli ATCC 80% Effective in 5 minutes >5.15 10536 The results show that the tested sample exhibited 5 logarithmic values ​​for all of the bacterial species in question. It shows that it provides microbial reduction on the unit. The fungicidal efficacy study was conducted at a product concentration of 80% by volume, on contaminated surfaces. This was carried out under the conditions and with a contact time of 15 minutes, according to the EN 1650 method. The microorganisms Candida albicans and Aspergillus brasiliensis were used in the study. It has been used. Product Contact Logarithmic Test microorganism evaluation concentration duration decrease value Candida albicans ATCC 15 80% >4.13 Effective 10231 minutes Aspergillus brasiliensis 15 80% >4.05 Effective ATCC 16404 minutes The results showed that for both microorganisms, 4 logarithmic units were involved. A decrease was observed and the sample was found to be effective in terms of fungicidal properties. It has been evaluated. Virucidal efficacy test according to EN 14476+A2 method, directly under contaminated conditions. at a sample concentration of 97%, at a temperature of 20°C and for five minutes This was carried out with regard to contact time. In the study, adenovirus type 5, poliovirus type 1, and murine Norovirus and Vaccinia virus were examined. Product Contact Logarithmic Reduction Virus tested and evaluated. concentration duration value Adenovirus Type 5 97% 5 minutes 4.50 Suitable Poliovirus Type 1 97% 5 minutes 4.50 Suitable Murine norovirus 97% 5 minutes 4.00 Suitable Vaccinia virus 97% 5 minutes >4.00 Suitable Ankara strain The experimental results showed that the tested pyrrolignoous acid-based sample was effective for four weeks under the specified conditions. It provides at least a logarithmic unit reduction against the entire virus strain and It has been shown to exhibit virucidal activity. 11

Claims

1- The invention concerns a method for producing a pyrrolignoous acid-based solution derived from olive pits. Its characteristic is;  olive pits in an oxygen-free or oxygen-restricted environment subjected to pyrolysis at a temperature between 400°C and 700°C in the environment,  Concentration of volatile components formed during pyrolysis and pyrrolignoous acid separation of the liquid fraction containing,  allowing the liquid fraction in question to settle,  Separation of the tar phase from the settled liquid fraction,  Pyrolignomous liquid fraction separated from the tar phase, filtered and purified. obtaining acid,  Mixing the obtained purified pyrrolignoous acid with water,  the addition of at least one organic or mineral acid component to the mixture in question and  Mixing the components until a homogeneous solution is obtained, process It includes the steps. 2- The production method mentioned in Claim 1, characterized by the separation of liquid from the tar phase. multiple fractions using filters with different pore sizes It is characterized by undergoing a filtration stage. 3- The production method mentioned in any of the previous requests, its characteristic is; word the subject is an organic or mineral acid component, at least one of which is phosphoric acid or citric acid. It is characterized by its... 4- The production method mentioned in any of the previous requirements, and its characteristic is; Purified pyrrolignoous acid, water, and the acid component are mixed at a temperature between 15°C and 35°C. It is characterized by being mixed at a certain temperature. 12 5- The production method mentioned in any of the previous requests, its characteristic is; one liter The solution consists of 250 mL of purified pyrrolignoous acid, 50 mL of phosphoric acid, and 700 mL of pure water. It is characterized by being prepared using [methods]. 6- Agricultural use of pyrrolignoous acid-based solutions produced according to any of claims 1-5. It is a method of use, and its characteristic feature is that the solution in question is added to irrigation water, hydroponics. Added to the solution or growing medium at a rate of 0.05% to 5% by volume. This involves regulating the pH and / or electrical conductivity values. 7- Fruit prepared using a pyrrolignoous acid-based solution produced according to any of claims 1-6. or a method of use in cleaning vegetables, its characteristic feature is; When the solution comes into contact with the fruit or vegetable, the fruit or vegetable in question the solution should be kept in contact for 5 minutes, and the contact time should be... After completion, rinsing the fruit or vegetable with water is the next step in the process. It includes. 8- The invention is a solution based on pyrrolignoous acid derived from olive pits, the characteristic of which is; The tar phase was separated and the filtered purified pyrrolignoous acid, water and the aforementioned It contains at least one organic and mineral acid component in addition to pyrrolignoous acid. 13