Remotely controllable and programmable biomedical isolated organ bath measurement system.

Abstract

The invention relates to an isolated organ bath used for the examination of tissues or organs outside the body, providing autonomous dosing, remote programming, and control. Figure 1

Description

1 TARIFF Remotely controllable and programmable biomedical isolated organ bathroom measurement system TECHNICAL FIELD The invention generally refers to keeping tissues or organs alive outside the body. It relates to isolated organ baths that provide this. The invention is particularly useful for examining tissues or organs outside the body. Isolated organ bath providing autonomous dosing, remote programming and control. It is related to. STATE OF THE ART 15 An isolated organ bath (isolated tissue bath) is a bath that uses a small sample taken from a living animal. a special process that allows a piece of tissue or organ to remain alive outside the body It is a laboratory device. In this system, tissue is placed in a container with appropriate temperature and nutrient fluids. It is placed in a reservoir. The purpose of this system is to deliver the drugs to the muscles or blood vessels. 20 to observe the effects on; the tissue's response to electrical or chemical stimuli The purpose is to measure. Isolated organ baths are commonly used in studies of smooth or striated muscle. It is preferred. The system mentioned, in its most general form, involves placing a special substance inside that is at body temperature. injecting a saline solution (such as Krebs solution), providing oxygen to the tissue, and 25 Transmitting muscle contraction or relaxation movements to a screen or recording device It operates on the principle of... Isolated organ baths are currently used in pharmacology and physiology research. These systems operate largely on the principle of manual operation. Change of Krebs solution in tissue bath in systems, drug (agent) 30 The applications, gas flow rate settings and temperature monitoring are done manually by the researcher. This is being monitored. These are the fundamental shortcomings that these current practices bring with them. And the technical problems are as follows: 2 - Physical dependence and loss of productivity: Autonomous control in current systems. Because the mechanism was not present, the researcher did not use the device during the experiment. The researcher must be physically present at the beginning. It significantly reduces efficiency and makes parallel work impossible. It makes it possible. 5 - Dosage and repeatability issues: Manual administration of drugs (agents). Dosing it by pipetting can lead to user-induced dose inconsistencies. This opens up the possibility of problems, particularly regarding the reliability of sensitive dose-response curves. It weakens it technically. - Temperature instability and thermal deviations: Most current systems have 10 The heating methods used are insufficient to maintain a constant temperature inside the bathroom. It remains. During the experiment, the temperature fluctuation was between ±0.5-1 °C. Deviations directly affect the physiological responses of the tissue, thus altering the data. This undermines the accuracy of a precise temperature regulation mechanism. Its absence makes the reproducibility of the experiment technically difficult. 15 - Risk of tissue damage: Tissue damage during manual fluid exchange. There is a high risk of mechanical damage. - Data analysis and statistical errors: Manual analysis of data in current systems This can lead to user error in integral and amplitude calculations. This can open the file and prolong the analysis time. Also, 20 different agents... The ability to compare the graphs in real time is technically limited. The current technology, document EP1317175A2, aims to preserve the viability of the organs. and / or the device used for recovery. This document is basic. 25 A perfusion device developed for this purpose has been described. The aim of this device is to deliver perfusion to the organ. The goal is to ensure the animal remains healthy until the transplant process. This device features autonomous dosing. Features such as remote programming and control were not mentioned. Document number TR202014402 relates to live whole blood organ baths. This 30 document on keeping an organ alive outside the body during organ transplantation processes and It describes a device that provides nutrition. This device also features autonomous dosing. Features such as remote programming and control were not mentioned. 3 As a result, improvements are being made to organ bathing devices, therefore It will eliminate the disadvantages mentioned above and provide solutions to existing systems. New structures are needed to bring about this. PURPOSE OF THE INVENTION 5 The present invention meets the aforementioned requirements and overcomes all the disadvantages. It relates to isolated organ bathing, which eliminates the need for oral hygiene and offers some additional advantages. The main purpose of the invention is to create isolated organ specimens for the examination of tissues or organs. autonomous dosing in the bathroom with remote programming and control features to provide. One purpose of the invention is to provide the researcher with remote monitoring and control capabilities in the laboratory. The aim is to eliminate the need to wait at the beginning. 15 Another aim of the invention is to solve the problems of solution replenishment through autonomous dosing. dissolution, sequential and precise drug administration without human intervention to ensure its realization. Another purpose of the invention is to prevent data analysis errors and wasted time. The goal is to create a system that processes raw data. All the advantages mentioned above and explained in detail below. The present invention aims to achieve; the experimental use of tissues or organs in the body 25 It is an isolated organ bath used to keep organs alive outside the body; • Enables remote monitoring and visual intervention in the experimental process camera unit, • drawing the agent / drug solution in a precise volume and placing it in the developing tank. the dosing unit that enables its delivery, 30 • controls the system's operation, signal processing, internet connection, and all central processing unit that coordinates mechanical automation A structure containing these elements has been obtained. 4 The structural and characteristic features and all the advantages of the invention are given in the figures below. And thanks to the detailed explanation written with references to these figures, it becomes clearer. This will be understood as such. Therefore, the evaluation should also be based on these forms and details. This should be done taking the explanation into consideration. BRIEF DESCRIPTION OF THE FIGURES The best way to utilize the advantages of the existing invention, together with its structure and additional elements. For understanding, it should be evaluated together with the figures explained below. is necessary. Figure 1 shows a general view of the isolated organ bath, which is the subject of the invention. 10 REFERENCE NUMBERS 1. Gas cylinder 2. Heating unit 3. Bathroom basin 15 4. Waste unit 5. Camera unit 6. Load sensing unit 7. Support plate 8. Supporting column 20 9. Dosing unit 10. Movement lever 11th Agent Unit 12. Central Processing Unit 13. Valve unit 25 DETAILED EXPLANATION OF THE INVENTION This detailed explanation describes the preferred method for the isolated organ bath, which is the subject of the invention. their structures are solely for the purpose of better understanding the subject and no 30 It is explained in a way that will not create a limiting effect. The invention involves keeping tissues or organs alive outside the body for experimental purposes. used, • Gas cylinder that provides the necessary oxygenation for maintaining tissue viability (1), • Heating that provides heat to the agent / drug solution into which it is placed in the tissue. 5 unit (2), • bath tank in which the tissue is placed and suspended (3), • Waste unit (4) into which the waste solution used in the bath tank (3) is emptied, • load that measures the contraction and relaxation of muscle tissue on the tissue sensing unit (6), 10 It is an isolated organ bath containing; • Enables remote monitoring and visual intervention in the experimental process camera unit (5), • drawing the agent / drug solution in a precise volume and into the bath tank (3) Dosing unit (9) that enables its transmission, 15 • controls the system's operation, signal processing, internet connection, and all Central processing unit that provides coordination of mechanical automation (12) It includes. Figure 1 shows a general view of the isolated organ bath, which is the subject of the invention. 20 In the isolated organ bath in question, the organ to be experimented on is a bath chamber. (3) is placed inside. The bath tank (3) is made of heat and impact resistant glass. It is a tank made of material. The bath tank (3) is also double-walled. It is structured in such a way that the bath temperature is maintained thanks to heated water circulated between the walls. It is kept stable. The vitality and normal functions of the tissue or organ are maintained for 25 minutes. In order to maintain it, the agent / medicine solution (Krebs solution) is placed in the bath tank (3). The solution in question is placed in a heating chamber (3) before it reaches the bath tank. Heating unit (2) is heated. Heating unit (2) has a spiral glass tube structure. Again, it is necessary to maintain the vitality of the tissue placed inside the bath tank (3). Oxygen is supplied by a gas cylinder (1) in the system. 30 inside the gas cylinder (1) oxygen is supplied to the bath tank via a pressure regulator and flow control line (3) The gas is conveyed to the distributor at the base. The tissue inside the bath tank (3) is a It is connected to a load sensing unit (6) with a coupling element. The mentioned load 6 sensing unit (6), contraction and relaxation on tissue throughout the experiment It is a sensitive sensing unit that measures movements and converts them into analog electrical signals. In an isolated organ bath system, all mechanical and electronic components of the system It is fixed on a support plate (7). There are 5 on the support plate (7). The dosing unit (9) draws the solution and puts it into the bath tank (3) It ensures that it is printed. To ensure this, the dosing unit (9) preferably, It is a miniature syringe pump driven by a stepper motor. Solutions are pumped through a support plate. (7) is kept in the agent unit (11). Agent unit (11) is in sequence. It consists of a series of capillary tubes arranged for dosing. 10 in the agent unit (11) Solutions are drawn from the tubes by a lever (10). Between the lever arm (10), the dosing unit (9) and the agent unit (11) positioned and dosing unit (9) of the solution taken from agent unit (11) It ensures that it is transferred to the bath tank (3) via the bath tank (3). Pumping the solution into it or emptying the used solution, a valve unit 15 (13) is provided. The solution used in the bath tank (3) is supplied by the valve unit. (13) is discharged into the waste unit (4). The load on the support plate (7) sensing unit (6), movement arm (10) and other upper units, carrier columns (8) ensures that it is kept at a constant height and level. Again, support The system can be monitored with the camera unit (5) connected on the plate (7) 20 is provided. The operation of the isolated organ bath system is controlled by the central processing unit (12). is controlled. The central processing unit (12) is the brain of the system; load The sensing unit (6) digitizes the signal in high resolution, microcomputer 25 (Raspberry Pi-based) signal processing (DSP / FFT, integral, amplitude), internet It manages the connection and coordination of all mechanical automation. Central There is a control panel on the processing unit (12). The mentioned control The panel automatically calculates amplitude, frequency, and integral values ​​by analyzing the signals. It is a web-based software that calculates and presents comparative analysis with multiple graphs. 30 The working principle of the isolated organ bath, which is the subject of this invention, is as follows: With the controlled gas flow initiated through the gas cylinder (1), the gas exiting is under pressure. through the regulator and flow control line, placed on the base of the bath tank (3) 7 The gas is conveyed to the diffuser, ensuring oxygenation of the tissue environment. In time, the thermocirculator (heater circulator) heats the water to the heating unit (2) and The bath tank (3) is passed through the jackets. Agent / drug solution before entering the bath. first it is preheated in the heating unit (2) and bath tank (3) at the target temperature (37°C, ±0.1°C) is stabilized. The solution is transferred to the bath tank (3) and used 5 The discharge of the liquid is managed by the valve unit (13). Valve in the supply line When opened, the solution fills the bath tank (3) by gravity; the valve in the drain line When opened, the used solution is discharged into the waste unit (4) by gravity. The automatic wash-out cycle is complete. The smooth muscle being experimented on... The fabric is hung on the hanging hook inside the bath basin (3) and a thin binding cord / wire 10 through the load sensing unit (6) fixed on the carrier column (8) It is mechanically connected to its movable arm. All these mechanical and electronic units It is mounted on the support plate (7). During the application phase of the experiment The user defines the dosing protocol via the web-based control panel. The lever (10) moves with the motor / slide, the tip of the dosing unit (9) attached to it. He positions the needle into the corresponding tube in the agent unit (11) array. Dosing unit (9) The stepper motor retracts the device, drawing in a precise volume of the selected agent / drug solution. Then the lever (10) moves onto the bath tank (3) and the dosing unit (9) The solution is then injected into the bath tank (3). In this way, sequential and Precise dosage applications are performed without human intervention. The tissue is 20... The contraction-relaxation response it generates is analogously detected by the load sensing unit (6). It is converted into a signal; this signal is digitized and processed in the central processing unit (12). The experiment supervisor can monitor the entire process remotely with the camera unit (5). Thus The entire cycle, from temperature control to dosing, measurement and liquid exchange, It is carried out fully autonomously from outside the laboratory via remote access infrastructure. 25 In short, the entire process, from temperature control to dosing, data analysis to liquid... remote access infrastructure that does not require human intervention until the change is made Thanks to this, it is completed in a fully autonomous loop from outside the laboratory. The operating principle of the system described in the invention, in its most general form, is: 30 - receiving the analog signal from the load sensing unit (6), - the received signal is processed in the central processing unit (12) at high resolution digitization, - noise removal from the digitized signal using digital filtering (DSP), 8 - fast Fourier transform, trapezoidal integral and amplitude of the purified signal application of analyses, - Processed data is transferred to a web interface via MQTT / TCP-IP protocols for multiple users. presented comparatively with a graph It consists of process steps. 5

Claims

9 REQUESTS 1. Keeping tissues or organs alive outside the body for experimental purposes. used, • Gas that provides the oxygenation necessary for maintaining tissue viability 5 tube (1), • enables the heating of the agent / drug solution into which it is placed in the tissue. heating unit (2), • bath tank in which the tissue is placed and suspended (3), • Waste unit 10 where the waste solution used in the bath tank (3) is emptied (4), • load that measures the contraction and relaxation of muscle tissue on the tissue sensing unit (6), It is an isolated organ bath containing; its characteristic feature is; • Allows for remote monitoring and visual intervention in the experimental process. 15 camera unit (5), • drawing the agent / drug solution in a precise volume and placing it in the developing tank. (3) dosing unit (9) that enables transmission, • controls the system's operation, signal processing, internet connection, and central processing unit 20 that coordinates all mechanical automation unit (12) It includes.

2. This is an isolated organ bath conforming to Claim 1, characterized by its suitability for sequential dosing. Agent 25 is a series of capillary tubes in which agent / drug solutions are stored. It contains unit (11).

3. Isolated organ bath conforming to claim 1 or 2, and its characteristic is; dosing unit (9) and by positioning the agent unit (11) so that the right agent is picked up at the right time It includes a lever arm (10) that enables its implementation. 30 4. Isolated organ bath conforming to Claim 1, characterized by: solution feeding and bathing. opening and closing the drain lines of the reservoir (3) to carry out the washing process It includes a valve unit (13) that provides 35 5. Isolated organ bath in accordance with claim 1, its characteristic is; central processing unit (12) working on it, analyzing the signals to determine amplitude, frequency, and integral values. A control system that automatically calculates and provides comparative analysis with multiple graphs. It includes the panel.

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