SAMPLING DEVICE FOR VOLATILE ORGANIC COMPOUNDS FROM SKIN AND ITS OPERATING METHOD

Abstract

The invention relates to a sampling device and its operating method that enables the collection of volatile organic compounds (VOCs) emitted from human skin in an isolated sampling medium, with reduced impact from external pollutants and atmospheric interferences; this device can be vacuum-fixed to the skin and is compatible with solid-phase microextraction (SPME) fibers and / or thermal desorption tubes.

Description

1 TARIFF Sampling device for volatile organic compounds from skin. AND HOW THE DEVICE IN QUESTION WORKS Technical Field to Which the Invention Relates 5 The invention relates to volatile organic compounds (VOCs) released from human skin to externally generated substances. in an isolated sampling environment by reducing the impact of pollutants and atmospheric interference. a solid phase that can be fixed to the skin via vacuum and enables collection. Compatible with microextraction (SPME) fibers and / or thermal desorption tubes. It relates to a sampling device that can operate in this way and its operating method. 10 The invention is particularly suitable for gas chromatography-based analysis of VOCs from skin and sweat. Sampling for disease diagnosis, biomarker monitoring, toxicology, exposure analysis, and It is suitable for use in forensic analysis applications. State of the Art 15 Metabolic, physiological, and biochemical processes occurring in the human body. As a result, various volatile organic compounds are formed. In general These compounds, called volatile organic compounds or VOCs, are involved in respiration, through sweating, diffusion from the skin surface, and various body secretions, to the external environment. It can be given. The type of volatile organic compounds emitted from the human body and 20 The amount depends on the person's metabolic status, dietary habits, age, and medications they are taking. depending on environmental exposures and current physiological conditions This can vary. Therefore, the analysis of volatile organic compounds of body origin; biomarker research, monitoring of metabolic processes, and disease associations. Determination of chemical profiles, toxicological studies, environmental exposure 25 It is used in fields such as evaluations and forensic analyses, especially in leather and... volatile organic compounds released through sweat are processed without any invasive procedure. This allows for the sampling of these compounds in terms of analytical and clinical research. its importance is increasing. The skin is the largest organ of the human body that comes into contact with the external environment and contains water, electrolytes, role in the transfer of metabolic wastes and various organic compounds to the external environment It plays a role. The majority of sweat secreted from sweat glands consists of water. Sweat contains various ions, amino acids, fatty acids, metabolites, and volatile substances. 2 or semi-volatile chemical compounds may be present. In addition, some volatile organic compounds enter the bloodstream without being a direct component of sweat. It can pass through diffusion to the skin surface or remain on the skin surface. as a result of the metabolic activities of microorganisms on sweat and skin secretions This can occur. Therefore, a volatile organic compound (VOC) taken from the skin surface... Examples of compounds include substances secreted from sweat glands as well as those found on the skin surface. compounds resulting from chemical and microbiological processes that occur It may contain. An accurate profile of volatile organic compounds of skin origin. in order to determine this, gases emitted from the skin surface during the sampling phase It should be collected as selectively as possible. 10 from the skin surface One of the main difficulties encountered in sampling volatile organic compounds is, The target compounds are mostly found at low concentrations. Some compounds are found at low concentrations. Since the amounts released from the surface are very low, the sampling method used the element must have sufficient adsorption capacity and selectivity It is necessary. In addition, the volatilities, polarities and 15 of the sampled compounds are considered. Their chemical stability differs. Compounds with high volatility. while easily lost during sampling and sample transfer, lower Volatile compounds strongly adhere to the surface of the sampling material. It can be retained and then completely desorbed in the subsequent analysis stage. It may not be possible to detect them. Chemically unstable compounds, however, require long sampling times or 20... They can degrade, oxidize, or mix with other compounds during storage periods. This can change. In these situations, the analytical profile obtained from the skin surface... it does not accurately reflect the type and relative amounts of compounds actually released. This can lead to... In the known state of the art, volatile organic compounds from skin and sweat. sterile cloths, gauze, absorbent pads, polymeric films for collection purposes, Adsorbent materials and sampling tapes with different properties are used. In these methods, the skin sample taken is usually of an odorless or volatile compound. It is cleaned with a cleaning agent that is assumed to be free of contaminants, and after a certain resting period of 30 days. After a certain period, the absorbent or adsorbent material will come into contact with the skin surface. It is positioned in this way. The sampling material is placed on the skin for a specific period of time. It is held in place, during which time sweat is absorbed by the material and the skin Compounds released from the surface are expected to accumulate on the material. 3 After the sampling process, the material is removed from the skin surface and A glass vial, tube, or similar closed sample container is used to minimize sample loss. It is placed inside the container. In methods using sterile cloth or absorbent material. It is often possible to transfer volatile organic compounds directly to the analytical instrument. It is not. Therefore, the compounds retained on the sampling material are removed with a solvent. 5 extraction, conversion to gas phase by applying heat, or in the top of a closed vial It needs to be accumulated in the void. In solvent extraction methods, The sampling material is brought into contact with a suitable organic solvent, and the material The compounds present on it are allowed to pass into the liquid phase. The resulting extract... then analytical methods such as gas chromatography or liquid chromatography 10 is being investigated. However, the use of solvents leads to dilution of the sample, inclusion of solvent-derived impurities in the analysis and preparation of additional samples This makes the necessary processes necessary. Furthermore, the highly volatile compounds... It is possible to lose it during extraction with the solvent and sample transfer. In upper space sampling methods, the cloth or material that has come into contact with the skin is used. The adsorbent material is placed inside a closed vial, and the vial is kept at a specific temperature. It is kept in a waiting period. Due to the effect of the applied heat, the volatile substances on the material... Organic compounds are allowed to pass into the gas phase of the vial. The compounds accumulate in the gas phase. Compounds can be collected directly using a gas sampling system or solid phase 20 It can be collected on microextraction fibers. Solid-phase microextraction is a common method. They are referred to as SPMEs and are coated with volatile or semi-volatile compounds. It is based on adsorption or absorption on a fiber surface. The fiber is the vial. After being left in the upper cavity for a certain period of time, the gas chromatography device Compounds are placed in the injection section and held on the fiber surface at a temperature of 25. It is desorbed by this action and transferred to the chromatographic system. This method, It does not require the use of solvents and low concentrations of compounds can be applied to fibers. It is widely used in the analysis of volatile organic compounds because it can be concentrated on the surface. It is used in this way. The performance of the SPME fiber method depends on the fiber coating. type, coating thickness, sampling temperature, sampling time, vial volume, 30 moisture content of the sample and distribution coefficients of compounds between the fiber and the gas phase It depends on numerous variables such as those used in the analysis of skin samples. The fact that their chemical properties vary over a wide range allows for the integration of all fibers with a single fiber coating. This makes it difficult to maintain the equal effectiveness of the compounds. A specific fiber coating. 4 It exhibits high retention capacity for polar compounds, while it is less effective for non-polar compounds. It may show low effectiveness or the opposite may occur. Also It can take a long time for the fiber to reach equilibrium in the upper space of the vial, and In some applications, the process of release from the cloth and accumulation on the fibers takes hours. It can continue. Volatile compounds from the material that comes into contact with the skin 5 The time required for its release and collection on the fiber can be up to twenty-four hours. There are applications where it can be extended. Gas from the skin surface to a sampling material, from the sampling material to the vial Sequential material transfer from the gas phase to the SPME fiber and to the phase 10 These stages can lead to analytical losses. At each transfer stage A certain portion of the compounds can be retained on the system surfaces and released into the ambient air. It may be able to escape or completely separate itself from the sampling material. In particular, sufficient space is left in the upper void for compounds that are strongly adsorbed to the fabric surface. It is possible that the amount does not exceed [a certain limit]. However, highly volatile compounds should not exceed 15% of the fabric. removal from the skin, placement into a vial, or closing of the vial There is a risk of loss during this process. Therefore, the result of the analysis determined compound distribution differs from compound distribution released from the skin surface. This can be demonstrated. Long sample preparation and sampling times, chemical From this perspective, the degradation or reduction to immeasurable levels of unstable compounds 20 This can also be the reason. In the current state of the art, thermal methods are used for the collection of volatile organic compounds. Desorption tubes are also used. Thermal desorption tubes, Sampling containing one or more adsorbent materials 25 These are the elements. The gas sample is passed through the tube with the help of a pump, and Volatile organic compounds in the sample are retained on the adsorbent material. After sampling is complete, the tube is placed in a thermal desorption device. and the compounds accumulated on the adsorbent are heated in a controlled manner with a carrier gas. It is transferred to the gas chromatography system with the help of thermal desorption tubes 30 processing of large quantities of gas samples and low concentrations of compounds This allows for concentration. However, the adsorbent used... properties, gas flow rate, sampling volume, humidity, temperature, and compounds of the adsorbent The clinging behaviors on the surface significantly influence the analysis results. Appropriate adjustment of gas flow rate in dynamic gas sampling. It is necessary. If the flow rate is too high, volatile compounds If the sample is not sufficiently retained on the adsorbent, it passes through the tube and results in sample loss. It is possible for it to reach that level. However, a low flow rate may not provide sufficient sample volume. This may require a long sampling period to reach the target. Also, adsorbent 5 If the capacity is exceeded, the compounds that are initially held will escape from the tube's outlet. A fracture event, which can be described as such, may occur. Moist gas samples In terms of adsorption capacity, the accumulation of water vapor on the adsorbent affects the adsorption capacity. It can reduce and negatively affect the thermal desorption process. Human Gas samples taken from an environment close to the skin may contain high relative humidity. 10 Therefore, moisture content in samples taken with thermal desorption tubes Its impact needs to be taken into consideration. The most important aspect of sampling volatile organic compounds from skin and sweat is... One of the problems is environmental pollution. Cloth, pads or 15 placed on the skin The adsorbent material interacts not only with compounds released from the skin surface, but also with sampling. It also comes into contact with the air in the environment. Located in the sampling room. cleaning products, disinfectants, perfumes, cosmetics, plastic materials, adhesives, furniture, laboratory chemicals and other human-caused Volatile compounds can accumulate on sampling material. Environmental 20 Some of the compounds have the same or similar chemical structure as skin-derived compounds. because of what it may have, the source of these compounds cannot be definitively determined after analysis. Determination becomes difficult. The mixing of external atmospheric pollutants into the sample, This leads to misinterpretation of results and decreased credibility. This is possible. Sampling 25 is done to reduce the impact of environmental pollution. beforehand, ventilate the room, avoid using perfumes and cosmetics. containment, cleaning of the sampling area and control of ambient air. Practices such as taking samples are used. Samples taken from the ambient air. Blank samples are used to identify compounds present in the environment and in the skin sample. 30 of the detected compounds are of environmental origin. It helps in the evaluation. However, the control sample... Removing it does not prevent environmental compounds from mixing with the skin sample. Environment If the compound in the air is also released from the skin, the total amount determined in the analysis result... how much of the amount originates from the environment and how much from the skin surface 6 It may not be possible to separate them completely. Also, in the ambient air... Since the concentration of volatile compounds can change over time, a control sample should be used. It is possible that the skin sample does not exactly represent the same environmental conditions. Cleaning the skin area from which the sample was taken is also a sufficient solution in itself. It does not provide. The material itself used for cleaning purposes is volatile or semi-volatile. 5 It may contain volatile components, and these substances can be detected in subsequent analysis. This is possible. A certain waiting period is required after the cleaning process. its application reduces compounds resulting from cleaning agents. while being able to provide, the natural chemical structure of the skin surface and microorganisms can alter its balance. Excessive washing of the skin or substances with solvent properties 10 cleaning with these substances removes skin lipids and restores normal conditions. This can cause a change in the released compound profile. Therefore, sampling... While standardizing preparatory procedures is necessary, different individuals... complete standardization between different skin regions and different environmental conditions. It is difficult to achieve. 15 The amount of compounds released from the skin surface depends on temperature, humidity, airflow, and sweating. It also varies depending on factors such as speed. Increase in ambient temperature. It can increase the amount of sweating and the evaporation rate of some compounds. Low At higher temperatures, sweating may decrease and lower amounts of volatile compounds may be present. It can be collected. Airflow removes the gas layer on the skin's surface. By removing them, it can accelerate the dispersal of compounds into the environment. Sampling factors such as the person's movement during the period, changes in body temperature, and stress Factors can also affect the level of sweating. Therefore, in open environments... In the skin samples taken, 25 samples were taken from the same person at different times. Significant variability can occur between samples. Tape, bandages, etc. are used to secure absorbent materials such as diapers, pads, and similar materials to the skin surface. Elastic carriers or mechanical fasteners can be used. The fastening method is... This can affect the contact area of ​​the material with the skin and the pressure exerted on the skin. 30 If the sampling material does not make sufficient contact with the skin, sweat... The transfer of pressure to the material may be limited. Applying excessive pressure to the skin It can alter blood circulation, temperature, and sweating behavior on its surface. Additionally, the adhesives and tapes used may release volatile compounds or come into contact with the skin. 7 It is possible for it to cause irritation. The curvature and surface of different body parts. changes in characteristics such as the hand, arm, leg, or torso of the same sample material This makes it difficult to use it in the same way in different regions. The use of single-use sampling materials increases the risk of cross-contamination. While offering the advantage of reducing costs, it also requires a significant amount of consumables. This leads to increased consumption. New diapers, pads, for each person or sampling area. Vials, caps, and auxiliary materials may be required. A large number of In clinical trials and screening applications where the sample is processed, this situation affects the business. It increases costs and generates significant amounts of medical or laboratory waste. 10 Where specialized adsorbent cloths or sampling pads cannot be produced locally. In these situations, dependence on imports and supply problems may arise. Because special sterile wipes are single-use, the amount of waste and the process are reduced. costs have increased; there are economic and logistical difficulties in obtaining these materials. It is known that it can be encountered. 15 Analysis of volatile organic compounds from skin is mostly done by gas chromatography and gas This is carried out using gas chromatography-mass spectrometry techniques. chromatography measures the volatility and stability of compounds in a sample within a column. It enables separation based on excessive interactions. Mass spectrometry, on the other hand, is 20 Identification of compounds exiting the column by obtaining their mass spectra. These methods provide high resolution and sensitivity. The reliability of the results obtained together depends largely on sampling and the sample size. It depends on the preparation stages. It mixes with the sample during sampling. If an environmental compound is accurately detected by a sensitive analytical instrument, 25 Even the source of the compound can be incorrectly assessed. Therefore, the analytical device Its high sensitivity to contamination and substances during the sampling phase It alone does not eliminate their losses. In current techniques, sample collection involves placing the sample into a closed container for 30 minutes. transfer, release of compounds from sampling material, SPME fiber or Collection of the adsorbent on the tube and its analysis by gas chromatography are twofold. It involves numerous subsequent processing steps. Each additional step increases the analysis time. It prolongs the process and increases the likelihood of user-generated errors. The sample is from a container. 8 transferring the sample to another, the vial not being sealed tightly enough, the sampling time changes, variations in applied temperature, or a different position of the fiber within the vial Maintaining it in that position can lead to variability in the results. Some Sample collection and analysis procedures for these methods can be completed within one day. It can take a long time. Due to the long processing time, only 5 samples can be analyzed within the same day. reducing the number and difficulty of use in applications requiring quick results This constitutes a crucial aspect. Preserving the samples before analysis is also important. It carries. Cloths or pads containing skin samples can be left at room temperature for a long time. Leaving them to sit results in the loss of highly volatile compounds and chemical degradation. This can lead to deterioration. Low temperature storage losses are 10. While it can reduce the number of compounds, it does not provide complete protection against all compounds. Originating from the lid, septum, or walls of the sample container. It is possible for compounds to migrate into the sample. In addition, some volatile organic compounds... Compounds can be adsorbed onto vial surfaces or pass through the cap material. It can diffuse into the environment. For these reasons, the time between sampling and analysis should be 15 minutes. keeping it as short as possible and standardizing storage conditions is required. Regarding the association of skin-derived volatile organic compounds with diseases. In studies, biological variability is also an important factor to consider. 20 The same compound can be found in healthy individuals and in individuals with a specific disease. However, the amount or the profile it forms with other compounds may vary. Nutrition, medication use, exposure to cigarette smoke, physical activity, hygiene products, occupation. Factors such as habitat and living environment can alter the volatile organic compound profile. This Therefore, in order to conduct a reliable biomarker assessment, sampling is required. standardizing conditions as much as possible, reducing environmental impacts and the use of a large number of control samples is required. Sampling Uncertainties at this stage mean that the real differences between biological groups are not related to environmental factors. or methodological differences make it difficult to distinguish them. Sampling approaches in the known state of the art involve volatile substances derived from skin and sweat. While it makes it possible to determine organic compounds, the sampling area... being exposed to the surrounding atmosphere, allowing environmental pollutants to mix with the sample, Requirement for disposable absorbent or adsorbent material, multi-stage sampling. 9 preparation processes, long sampling and analysis times, transfer of compounds loss in stages and compounds with different chemical properties with the same efficiency It has limitations such as the inability to collect. Especially at low concentrations and unstable. Sampling medium control is necessary for reliable determination of compounds. reducing sample transfer stages, environmental initiatives 5 limiting and effectively preventing gases released from the skin surface of the sampling element Ensuring contact in this manner is important. Accordingly, from the skin surface... In techniques for sampling volatile organic compounds, reproducibility is crucial. increasing efficiency, shortening processing time, reducing environmental pollution, and different 10. Establishing working conditions compatible with analytical sampling elements Technical needs persist. Brief Description and Objectives of the Invention The invention describes how volatile organic compounds (VOCs) released from human skin can be absorbed into the external environment. By reducing the impact of source pollutants and atmospheric interference, an isolated sampling method 15 a solid-phase material that can be attached to the skin via vacuum and allows for collection in the environment. Compatible with microextraction (SPME) fibers and / or thermal desorption tubes. a sampling device that can operate in this way and the operating method of the said device It is explained. The purpose of the invention is to detect volatile organic compounds released from human skin in the external environment. The aim is to ensure that the samples are taken without being affected by pollutants from the source. Skin sampling the cell forms a sampling volume isolated from the external environment and the said a VOC isolation channel surrounding the cell, fed with inert gas By creating a buffer zone, it allows for sampling of atmospheric pollutants. Access to the cell is restricted. One aim of the invention is to ensure the sampling device is airtight and stable on the skin surface. The aim is to ensure that the device is fixed in place on the skin surface. Keeping the device securely on the skin; fixing vacuum inlet, vacuum channel connected to this inlet, vacuum pump, flow 30 through a vacuum line consisting of an adjuster, T-tube and pressure gauge This is provided. The negative pressure created by the vacuum pump affects the base of the device. While attaching to the skin, the flow regulator and manometer control the applied vacuum. It allows for this to be done. One aim of the invention is to enable sampling using a vacuum applied for fixation to the skin. Its purpose is to prevent the removal of volatile organic compounds from the cell. Vacuum channel VOC isolation channel placed between the skin sampling cell, sampling It forms a protective buffer zone between the volume and the vacuum line. Tedlar bag 5, which delivers VOC-free inert gas to the insulation channel. by connecting it, the gas is drawn in by the vacuum effect, not from the sampling cell. This can be achieved through the insulation channel. One aim of the invention is to enable samples to be taken from different parts of the human body. The aim is to ensure compatibility with different anatomical surfaces, the body to be sampled 10 obtained with a changeable base that can be selected according to the geometry of the region. It is made. The main base is airtight to the hands, arms or other body parts. Its shape allows the sampling cell to be used on different surfaces. It provides. Another aim of the invention is to remove volatile organic compounds directly from the skin surface. The aim is to provide a sample. It is designed to be in direct contact with the skin. Skin sampling cell; SPME passage channel, SPME inlet slot, gas inlet slot and It is connected to the gas release port. Thus, volatile compounds released from the skin surface, Direct sampling without the use of sterile gauze or similar intermediate medium 20 It can be obtained from its volume. Another purpose of the invention is to provide sterile swabs and similar disposable sampling materials. The goal is to eliminate the need for disposable materials. the requirement, compounds released from the skin surface in closed skin sampling cell 25 directly through the SPME fiber or thermal desorption tube This is reduced by collecting sweat. This structure involves first absorbing sweat into an absorbent material. It eliminates the need. Another aim of the invention is to shorten the sampling and sample preparation time. 30 The shorter duration is due to the fact that volatile compounds first collect on a cloth and then leave the cloth. obtained by eliminating the multi-stage process based on desorption The compounds are applied directly to SPME fiber or thermal desorption tubes. 11 its retention, a separate extraction or secondary desorption step It reduces the need. One aim of the invention is to address issues that occur during sample transfer and desorption stages. The aim is to reduce the loss of volatile compounds that may occur. Volatile organic compounds in the skin 5 from the surface directly to the sampling cell and from there to the SPME fiber or thermal Transferring the mixture to a desorption tube reduces the number of intermediate transfer steps. In this way, it can remain on the fabric surface or during sample transfer. It becomes possible to directly collect compounds that might otherwise be lost. One aim of the invention is the detection of low concentrations or unstable volatile organic compounds. The aim is to facilitate sampling. Shortening the sampling time allows for the sample to be collected between samples. SPME fiber, which is not transferred to a material and exhibits condensing properties, or The use of a thermal desorption tube allows for the collection of small amounts of compounds. This makes it easier. Thanks to this, materials that could deteriorate or absorb during long waiting periods... 15 risk of losing compounds that cannot be analyzed by remaining on the material is being reduced. Another aim of the invention is to create both solid-phase microextraction fibers and thermal The aim is to enable sampling using a desorption tube. Different sampling methods 20 Compatibility with components; screw-type SPME connector, insulation gasket housing, SPME gas passage channel, SPME inlet socket and thermal desorption tube to which it can be connected This is provided via the line. In the SPME method, the fiber system is connected via the SPME input socket. During installation, the TD tube is connected to the gas line in the thermal desorption method. One aim of the invention is to implement static and dynamic sampling options. The aim is to provide static or dynamic operation options; gas inlet valve, gas outlet valve. controlled gas flow consisting of a valve, gas sampling pump and inert gas source It is created with the system. In dynamic sampling, gas is collected by keeping the valves open. The sampling pump is running, and in static sampling, cleaning with inert gas is 30 minutes. Then the valves are closed, allowing the compounds to accumulate in the sampling volume. is provided. 12 One aim of the invention is to remove residual compounds within the device prior to sampling, and The aim is to remove any potential contamination. The system's sampling... preparation, gas inlet port, gas outlet port, inert gas source, valves and gas by passing inert gas through the internal volume of the device via a sampling pump This process is carried out to remove any residual gases that may have remained inside the device and 5 It serves to remove pollutants before sampling begins. One aim of the invention is to create a reusable and low-operating-cost sampling method. The aim is to provide a system with a reusable main body and a replaceable main base. Connection devices and skin sampling suitable for direct gas phase sampling 10 The cell allows the device to be used in multiple sampling processes. This also reduces the need for disposable sterile wipes, thus saving on consumables. to reduce consumption, generated waste and sampling costs per patient It contributes. Explanation of the Figures Figure 1. View of the device from different angles; a) Front view of the device, b) Side view of the device. from: c) the bottom of the device, d) the assembled body and cover of the device. their appearances. Figure 2. Flowchart of the system and sampling method. 20 Explanation of References in Figures 1. Main body 2. Gas inlet port 3. Gas outlet port 25 4. VOC isolation duct inlet 5. Vacuum inlet 6. SPME connector 7. Insulation gasket housing 8. SPME fiber transit channel 30 9. Main base 10. Vacuum channel 11. VOC isolation channel 12. Skin sampling cell 13 13. Gasket clamping cap 14. SPME fiber input port 15. Gas sampling pump 16. Vacuum pump 17. Tedlar bag 5 18. Gas inlet valve 19. Gas outlet valve 20. Thermal Desorption (TD) Tube 21. SPME fiber 22. Inlet block 10 23. Gas chromatography device 24. TD device 25. Flow regulator 26. T-pipe 27. Pressure Gauge 15 Detailed Description of the Invention The invention relates to the removal of volatile organic compounds (VOCs) released from human skin from the external environment. human skin allows for the isolation and sampling of pollutants originating from it. a compatible, reusable sampling device and the operation of that device 20 It is related to the method. The device that is the subject of the invention creates a leak-proof seal on the leather surface. By creating a sampling environment, direct solid-phase sampling of volatile compounds originating from the skin can be achieved. using microextraction (SPME) fiber or thermal desorption (TD) tube This allows for the collection of sterile gauze. Thus, the use of sterile gauze, on the gauze... Additional sample preparation such as adsorption followed by desorption from the gland 25 direct sampling from the skin surface without the need for further steps. It can be accomplished. Views of the main body of the device subject to the invention (1) from different angles are shown in Figure 1.a-1.d. It is shown. The device consists of a main body (1), 30 on the skin of the main body (1). a pre-replaceable base (9) and main body (1) that allows its placement It includes a gasket compression cap (13) located on the main body. (1), at least the gas used during sampling is introduced into the device. a gas inlet slot (2) and a sampling of the gas inside the appliance to the outside or to a sample 14 It includes at least one gas outlet slot (3) which enables transfer to the main body. (1) and base (9), using polylactic acid (PLA) filament in an application. PLA filaments are produced from raw materials such as corn starch, sugarcane, and These can be biologically derived materials such as sugar beet. However, the main body (1) and / or base (9), polytetrafluoroethylene (PTFE / Teflon) such as VOC 5 They can also be produced from inert materials that will not negatively affect sampling. The base (9) is the part of the device that contacts the skin area to be sampled. It consists of a VOC isolation channel inlet (4) on the main base (9). vacuum inlet (5), a vacuum channel (10), a VOC isolation channel (11) and a skin 10 There is a sampling cell (12). The main base (9) is the body to be sampled. The device can be selected to match the geometry of the skin surface. It is ensured that it fits airtight. For this purpose, the main base (9), hand, arm or the surface geometry of other areas of the body from which sampling is desired It can be produced in different shapes and sizes to accommodate. The main sole (9) is leather 15 The fact that it sits on the surface in a leak-proof manner is mainly created in the vacuum channel (10). This is achieved thanks to negative pressure; the main sole (9) is tightly pressed against the body surface It is pulled and adheres to the leather. There is also a gap between the main sole (9) and the leather surface. No gasket or flexible base / contact element is present. VOC insulation duct. (11) is filled with VOC-free inert gas and in case of a possible leak, the outside 20 a secondary gas that restricts the entry of ambient gases into the skin sampling cell (12) It forms a barrier. Skin sampling cell (12), placement of the main base (9) on the skin surface As a result, it creates a closed sampling volume between the skin and the device. (25) The subject is skin sampling cell (12), volatile compounds released from the skin surface accumulated and via SPME fiber (21) or thermal desorption (TD) tube (20) It is the section where the sample is taken. Isolation of the skin sampling cell (12) from the external environment. thanks to this, volatile compounds present in the ambient air are transferred to the sampling medium. This prevents it from entering and affecting the analysis results. In one application, the skin 30 Sampling cell (12) has base dimensions of 2.5 cm × 2.5 cm and a height of 3 cm. It is in the shape of a square prism and has a volume of approximately 18.75 cm³. These measurements are based on the applied method. It can be modified as needed depending on the region or purpose. The vacuum channel (10) located on the main base (9) directs fluid to the vacuum inlet (5) It is connected. By applying vacuum to the vacuum channel (10), the skin of the main sole (9) Pulling it towards the surface and fixing the device to the area to be sampled This is provided so that the device can also be fitted with a band, strap, cloth or disposable material. It is possible to adhere to the leather surface without the use of a fixing element. 5 Vacuum allows the device to adhere to the skin surface and the inert sampling medium. to ensure its protection, continuously throughout the sampling period is being implemented. The vacuum line that enables the device to be fixed to the skin surface; a vacuum pump (16), 10 It includes a flow regulator (25), a T-tube (26) and a manometer (27). The vacuum pump (16) is connected to the vacuum inlet (5) and the vacuum channel (10) is negative. It applies pressure. The flow regulator (25) regulates the flow created in the vacuum line. and / or allows adjustment of the negative pressure level. T-pipe (26), It allows the vacuum line to be connected to the manometer (27). Manometer 15 (27) enables monitoring of the negative pressure applied to the vacuum channel (10). This allows the device to be securely and controllably fixed to the skin surface. It provides a constant value for the negative pressure to be applied to the vacuum channel (10). or no mandatory pressure range is specified; negative pressure, the device's skin a 20 that will not separate from the surface but will not cause deformation on the skin surface The level can be adjusted. This negative pressure determines the sampling condition. Its main purpose is to secure the device to the skin surface. The negative pressure applied to the vacuum channel (10) in the skin sampling cell (12) absorbing the gas and causing a pressure change in the skin sampling cell (12) 25 to prevent bringing in a vacuum channel (10) and a skin sampling cell (12) There is a VOC isolation channel (11) that forms a buffer zone between them. VOC The insulation channel (11) is fluid-connected to the VOC insulation channel inlet (4). VOC A Tedlar bag containing VOC-free inert gas is placed at the entrance of the insulation channel (4). (17) is connected. In preliminary trials, a high 30 inert gas without VOC was used. Pure nitrogen was used. However, it was suitable for the target analyte and analytical conditions. Various high-purity, VOC-free inert gases can also be used. Inert gas The source is not limited to Tedlar bag (17) and can properly store inert gas Different gas sources can also be connected to the VOC isolation channel inlet (4). 16 Gas may occur during the application of vacuum to the vacuum channel (10). instead of gas being drawn from the skin sampling cell (12) from the Tedlar bag (17) VOC is counteracted by drawing inert gas into the insulation channel (11). In this way Disruption of the sampling medium in the skin sampling cell (12) by vacuum effect isolating the skin sampling cell (12) from the external environment is prevented. It is protected. VOC insulation channel (11) is also located in the external environment. transport of pollutants through vacuum channel (10) to skin sampling cell (12) It creates a gas buffer that blocks the flow. On the main body (1), a SPME fiber (21) is sealed to the main body (1) 10 There is an SPME connector (6) which is preferably screw-type and enables connection. An insulation gasket housing (7) on the SPME connection adapter (6), main body (1) Inside it is an SPME fiber transit channel (8). Seal compression head (13) has an SPME fiber input socket that allows the SPME fiber (21) to enter the system. (14) is located. SPME fiber (21) is passed through the SPME fiber input slot (14) 15 and through the SPME fiber passage channel (8) into the skin sampling cell (12) It is positioned to reach the sampling medium. Isolation gasket The gasket in the housing (7) is compressed by means of the gasket compression cap (13) SPME forms a gas-tight connection around the fiber (21). Thus, outside air enters the skin from the point where the SPME fiber (21) enters the system 20 either enters the sampling cell (12) or the gas in the skin sampling cell exits Escape is prevented. SPME fiber (21) will not come into contact with the skin surface. The skin is exposed to the gas phase collected in the sampling cell (12). SPME fiber coated with Carboxene / Polydimethylsiloxane (CAR / PDMS) in trials Although fiber type and coating material were used, the target analyte was 25% different. It can be modified according to its specifications. Prior to the sampling process, the skin surface was treated with cotton soaked in pure water in one application. It is cleaned with [method]. Different cleaning methods are used to prepare the leather surface. The procedures can be selected and implemented according to the target application. 30 An example setup for the use of the device described in the invention for sampling purposes. This is shown in Figure 2. Before the sampling process, the body to be sampled... A base (9) is selected that is suitable for the surface geometry of the region. The selected base 17 base (9), airtight contact around the skin sampling cell (12) It is placed on the skin surface in such a way as to create. Then a vacuum pump (16) is being operated and negative pressure from vacuum inlet (5) to vacuum channel (10) is applied. The applied negative pressure is monitored by means of a manometer (27). and is adjusted to the appropriate level with the help of the flow regulator (25). Thus, the device is 5 It is fixed firmly and securely to the leather surface. Sampling of the main sole (9) It can be produced in different shapes and sizes according to the surface geometry of the area to be constructed. Thanks to this, the device can be applied to different skin types of the body using a suitable head / base. They can be used for sampling purposes on surfaces. During the vacuuming process, the gas in the skin sampling cell (12) is removed by vacuuming. VOC insulation channel inlet to prevent it from being drawn into the channel (10) (4) is connected to a Tedlar bag (17) containing VOC-free inert gas. Vacuum Gas demand resulting from its effect from Tedlar bag (17) to VOC insulation channel (11) is met by the transferred inert gas. Thus, skin sampling 15 The internal volume of the cell (12) is gas-isolated from the vacuum line and the external environment. is being done. Sampling in order to remove possible contaminants from the sampling system. Before the process, preferably VOC-free inert gas 20 from the gas inlet port (2) Inert gas is given by passing it through the skin sampling cell (12) and gas exit It is transferred out of the system from its housing (3). In preliminary tests, the cleaning process takes 50 The cleaning process was performed for 60 seconds at an inert gas flow rate of mL / min. Duration and flow rate depend on the type of target analyte, instrument volume, and clinical / experimental requirements. It can be modified according to the results obtained from the applications. 25 To ensure that inert gas is passed through the device in a controlled manner, gas a gas inlet valve (18) on the inlet line and a gas outlet valve (19) on the gas outlet line The gas inlet valve (18) supplies inert gas to the skin sampling cell (12). It controls the flow of gas; the gas outlet valve (19) is located 30 from the skin sampling cell. (12) the gas coming out is sent to the gas sampling pump (15), TD tube (20) or outside the system It ensures the redirection of gas. Gas flow through the system is facilitated by this process. A gas sampling pump (15) can be connected to the outlet line. Gas sampling pump (15) discharges the gas from the skin sampling cell (12) through the gas outlet slot (3) 18 It creates a controlled gas flow in the system by drawing gas samples. In applications where the pump (15) is not used, a pressurized gas inlet can be placed in the gas inlet (2) By connecting an inert gas source, the inert gas is passed through the system at the desired flow rate. It is possible to pass through the gas inlet valve (18) and gas outlet valve (19). 5 that can be manually adjusted and controlled by the user. It can be adjusted. Vacuum pump (16), gas sampling pump (15) and Valve control can be performed manually, or the system can control these elements. It can also be carried out in a fully automated system where it is automatically controlled. The device can perform static or dynamic sampling. Dynamic 10 During sampling, the gas inlet valve (18) and gas outlet valve (19) are in the open position. is kept and the gas sampling pump (15) is operated during the sampling period. Thus, inert gas is passed through the skin sampling cell (12) in a continuous or specified flow. volatile compounds are passed through the SPME fiber at speed and released from the skin surface (21) or collected on TD tube (20). During static sampling, 15 Gas inlet following removal of pollutants from the system using inert gas. valve (18) and gas outlet valve (19) are closed. By closing the skin sampling cell (12) it becomes a closed sampling volume. and the specified sampling period of volatile compounds released from the skin surface During this time, SPME 20 is allowed to accumulate in the skin sampling cell (12). fiber (21) adsorbs compounds in the gas phase in the skin sampling cell (12) It is either absorbing or retaining it. In preliminary trials, dynamic sampling was 50 mL / min. gas flow rate sampling for 600 seconds, and static sampling for 600 seconds. This has been carried out. The sampling periods and dynamic sampling in question... The gas flow rate used is not constant and will be determined based on the target analyte and patients. It can be adjusted according to the results of the studies. If sampling is done with SPME, SPME fiber (21), gasket compression By passing through the SPME fiber input slot (14) located in the heading (13), the SPME transition It is advanced along the channel (8) and sampling in the skin sampling cell (12) 30 They are exposed to the environment. After the completion of the determined sampling period... then the SPME fiber (21) is removed from the instrument and the gas chromatography instrument (23) is placed in the inlet block (22). SPME fiber (21) is held on it. The compounds are thermally desorbed at a suitable temperature in the inlet block (22) 19 and is analyzed in a gas chromatography device (23). Thermal desorption (TD) Thermal desorption (TD) when sampling is done using tube (20) tube (20), gas coming out of skin sampling cell (12) in gas outlet line It is positioned so that the gas sampling pump will pass through it. (15) contains volatile compounds released from the skin surface as a result of the flow it creates. The gas is passed through a thermal desorption (TD) tube (20) and the compounds in question are On the adsorbent material inside the thermal desorption (TD) tube (20) is retained. Thermal desorption follows the completion of the sampling process. (TD) tube (20) connected to the gas chromatography device (23) TD device (24) Compounds retained on the thermal desorption (TD) tube (20) are placed. TD 10 desorbed at a suitable temperature in the device (24) and transferred to the gas chromatography device (23) is analyzed by transferring. In preliminary trials, thermal desorption (TD) tube (20) Tenax TA was used as the adsorbent material in it; however The adsorbent material in the TD tube depends on the properties of the target analyte. It can be changed. 15 In a preliminary trial, the sampling process was carried out for 10 minutes at 720 mmHg. This experiment was conducted under pressure conditions and at a flow rate of 50 mL / min. The parameters are not final and limiting and will be performed on patients. It can be adapted according to the results of the studies. 20 The invention allows the device to take direct samples from the skin surface. In sampling methods performed using sterile gauze, the required gauze is... application to the skin surface, retention of sampled compounds on the cloth and more Then the steps of desorbing from the cloth are eliminated. Thus, example 25 Preparation and analysis time is shortened, and problems that occur during desorption are minimized. Potential compound losses are reduced, and compounds are adsorbed onto the fabric surface and desorbed. analysis of compounds that cannot be detected or that may degrade during the sample preparation process It becomes possible to do so. The device’s vacuum channel (10), VOC isolation channel (11) and skin sampling cell (12) thanks to its structure which functionally distinguishes it from the skin surface The integrity of the sampling medium is preserved during vacuum stabilization. Providing inert gas to the VOC isolation channel (11) in the skin sampling cell (12) prevents volatile compounds from being transported into the vacuum line and the external environment It reduces the impact of pollutants on the analysis results. The operating principle of the device described in the invention is as follows: i. Determining the body region to be sampled and selecting the body in question. 5 main Selection of the base (9), ii. air around the selected base (9), skin sampling cell (12) The skin will be sampled in a way that creates a leak-proof contact. placement on the surface, 10 iii. VOC insulation channel inlet (4), containing VOC-free inert gas Tedlar bag (17) or another gas that can store inert gas connecting the source, iv. Connecting the vacuum pump (16) to the vacuum inlet (5) and vacuum By operating the pump (16), negative 15 is poured into the vacuum channel (10). By applying pressure, the main sole (9) is pulled towards the leather surface, word the subject is the continuous application of vacuum throughout the sampling period, v. negative pressure applied to the vacuum channel (10) manometer (27) by monitoring and adjusting with the help of flow regulator (25) The device must be securely and tightly fixed to the skin surface at -20°C. the pressure will prevent the device from separating from the skin surface, but will still adhere to the skin. adjusting it to a level that will not cause deformation vi. The gas requirement that occurs during vacuum application is met by Tedlar with inert gas transferred from bag (17) to VOC insulation channel (11) by being met from the vacuum line of the skin sampling cell (12) and outside 25 isolating it from the environment, vii. Removal of pollutants that may be found in the sampling system In order to open the gas inlet valve (18) and the gas outlet valve (19) bringing in, supplying VOC-free inert gas from the gas inlet port (2) and the inert gas in question is passed through the skin sampling cell (12) and gas 30 Transfer from the output socket (3) to outside the system, viii. sampling process using SPME fiber (21) or Thermal It will be carried out using a desorption (TD) tube (20) and 21 whether sampling will be conducted under static or dynamic sampling conditions determination, ix. If sampling is done with SPME fiber (21), the SPME fiber (21) from the SPME fiber inlet slot located in the gasket compression cap (13) (14) is passed through the SPME passage channel (8) and the skin 5 exposure to the sampling medium in the sampling cell (12) and will create a gas-tight connection around the SPME fiber (21) In this way, the gasket is compressed by means of the gasket compression cap (13), If sampling is done with x. thermal desorption (TD) tube (20), Skin sampling 10 in the gas outlet line of the thermal desorption (TD) tube (20). so that the gas coming out of the cell (12) will pass through it positioning, xi. In the case of static sampling, the pollutants present in the system After removal with inert gas, the gas inlet valve (18) and the gas Skin sampling by closing the outlet valve (19) 15 making the cell (12) into a closed sampling volume and skin specified sampling period of volatile organic compounds released from the surface accumulation in the skin sampling cell (12) throughout, xii. In the gas phase of the skin sampling cell (12) during static sampling 20 Volatile organic compounds accumulated are adsorbed by SPME fiber (21) or absorption, xiii. In case of dynamic sampling, the gas inlet valve (18) and the gas keeping the outlet valve (19) in the open position and gas sampling skin sampling of inert gas by operating the pump (15) passing through the cell (12) at a continuous or specified flow rate, 25 xiv. Volatile organic compounds released from the skin surface during dynamic sampling Contact of gas containing compounds with SPME fiber (21) or gas outlet passing through the thermal desorption (TD) tube (20) located in the line In this way, the volatile organic compounds in question can be applied to SPME fiber (21) or Collection on thermal desorption (TD) tube (20), 30 xv. After the completion of the specified sampling period, SPME fiber (21) If SPME fiber is used from the (21) device removal and inlet block (22) of the gas chromatography device (23) placement, 22 xvi. Volatile organic compounds retained on SPME fiber (21) in the inlet block (22) thermal desorption at a suitable temperature and gas analysis in the chromatography device (23), xvii. In case a thermal desorption (TD) tube (20) has been used Thermal desorption (TD) 5 after the completion of the sampling process Removal of tube (20) from gas outlet line and gas chromatography Placement of the TD device (24) connected to the device (23), xviii. Adsorbent material in thermal desorption (TD) tube (20) The volatile organic compounds retained on the TD device (24) are suitable desorb at temperature and put into gas chromatography apparatus (23) 10 by transferring and analyzing it, xix. After the sampling process is completed, the vacuum pump (16) stopping, removing the device from the skin surface and reusing it disconnecting the device connections It includes the steps of the process. 15 The invention involves a reusable device and during sampling... A sterile swab or similar disposable sample collection device is needed. Not hearing it reduces consumable consumption and operating costs. Thanks to the interchangeable base (9), it is leakproof from different body parts 20 Sampling can be performed using SPME fiber (21) and thermal desorption (TD) tube. Thanks to its structure compatible with (20), different volatile compounds can be static or dynamic. It is possible to sample under these conditions. Industrial Applicability of the Invention 25 The invention relates to the removal of volatile organic compounds (VOCs) released from human skin from the external environment. human skin allows for the isolation and sampling of pollutants originating from it. a compatible, reusable sampling device and the operation of that device It relates to the method and is applicable to industry. The invention is not limited to the above descriptions, and a person skilled in the field can easily make further discoveries. It can demonstrate different applications of the invention. These are the claims and demands of the invention. It should be evaluated within the scope of the protection granted.

Claims

23 REQUESTS 1. Sampling of volatile organic compounds (VOCs) released from human skin. It is a sampling device with the following features: • at least one gas inlet port (2), at least one gas outlet port (3) and an SPME a main body (1) with a passage channel (8), 5 • connected to the main body (1) and on the leather surface a deployable base (9), • a vacuum inlet (5) located on the main base (9), the vacuum in question a vacuum channel (10) with fluid connection to its inlet (5), a VOC isolation channel inlet (4), fluid 10 to the inlet of the VOC isolation channel (4) a connected VOC isolation channel (11) and a closed system together with the skin surface a leather located on the main base (9) in such a way as to create volume Sampling cell (12), • between vacuum channel (10) and skin sampling cell (12) The VOC isolation channel (11) is located in 15 • an SPME connection adapter (6) located on the main body (1), said The subject is an insulation gasket located on the SPME connection apparatus (6). nest (7), • having an SPME fiber entry slot (14) and an insulation gasket slot (7) a gasket compression cap (13) and 20 connected with the gasket • a vacuum pump (16) connected to the vacuum inlet (5) It includes.

2. According to claim 1, it is a sampling device, the characteristic of which is; the main body (1) and / or main the base (9) is an inert such as polylactic acid (PLA) or polytetrafluoroethylene (PTFE) It is made of the following material: 25 3. Sampling device according to claim 1, its feature is; skin sampling cell (12) It has base dimensions of 2.5 cm × 2.5 cm, a height of 3 cm, and a volume of 18.75 cm³. It is a square prism or of different geometric dimensions and shape.

4. According to claim 1, it is a sampling device and its feature is; vacuum pump (16) vacuum a flow regulator (25) of the vacuum line connecting to the inlet (5), a T pipe 30 (26) and includes a manometer (27). 24 5. Sampling device according to claim 1, its characteristic is; VOC isolation channel inlet. (4) a gas source containing connectable, VOC-free inert gas It includes.

6. According to claim 5, it is a sampling device, the characteristic of which is that it measures the gas source in question. Tedlar bag (17) and the inert gas in question is high purity nitrogen such as 5 It is an inert gas.

7. It is a sampling device according to Claim 1, and its characteristic is; SPME fiber input port. (14) and from the SPME fiber passage channel (8) to the skin sampling cell (12) extending and without touching the skin surface, skin sampling cell (12) It contains an SPME fiber (21) located in the gas phase. 10 8. According to claim 7, it is a sampling device and its feature is that it is an SPME fiber (21) Carboxene / Polydimethylsiloxane (CAR / PDMS) or other types of coated It is the fact that.

9. According to claim 1, it is a sampling device and its feature is; fluid into the gas outlet slot (3). a connected gas outlet line and 15 on said gas outlet line It includes a positioned thermal desorption tube (TD) (20).

10. Sampling device according to claim 9, its characteristic is thermal desorption (TD). The tube (20) contains Tenax TA or a different adsorbent material.

11. According to claim 1, it is a sampling device and its feature is that it is connected to the gas inlet socket (2). A gas inlet valve (18) located on the gas inlet line connects to the gas outlet socket 20 (3) a gas outlet valve (19) located on a connected gas outlet line and word The subject is that it includes a gas sampling pump (15) connected to the gas outlet line.

12. Sampling of volatile organic compounds (VOCs) released from human skin. The working method and characteristic of a sampling device is; i. Determining the body region to be sampled and the relevant 25 having a shape and dimensions that match the surface geometry of the body region Selection of the main base (9), ii. around the selected base (9), skin sampling cell (12) Sampling will be done in a way that creates an airtight contact. placement on the skin surface, 30 iii. VOC insulation channel inlet (4), containing VOC-free inert gas Tedlar bag (17) or another gas that can store inert gas connecting the source, iv. Connecting the vacuum pump (16) to the vacuum inlet (5) and vacuum negative vacuum channel (10) by operating the pump (16). by applying pressure, pulling the main sole (9) towards the leather surface, the continuous application of the vacuum in question throughout the sampling period, v. negative pressure applied to the vacuum channel (10) manometer (27) 5 by monitoring and adjusting with the help of flow regulator (25) the device must be securely and tightly fixed to the leather surface, Negative pressure will prevent the device from separating from the skin surface, however adjusting it to a level that will not cause skin deformation vi. The gas requirement during vacuum application is met by Tedlar 10 with inert gas transferred from bag (17) to VOC insulation channel (11) met by the vacuum line of the skin sampling cell (12) and outside isolating it from the environment, vii. Removal of pollutants that may be found in the sampling system For this purpose, the gas inlet valve (18) and the gas outlet valve (19) are open 15 bringing to position, VOC-free inert gas from the gas inlet socket (2) and the inert gas from the skin sampling cell (12) By passing through the gas outlet (3) it is transferred out of the system, viii. sampling process using SPME fiber (21) or thermal It will be carried out using a desorption (TD) tube (20) and 20 sampling in static or dynamic sampling conditions determining that it will be carried out ix. If sampling is done with SPME fiber (21), the SPME fiber (21) from the SPME inlet slot (13) located in the gasket compression head (14) is passed through the SPME fiber passage channel (8) and the skin 25 exposure to sampling medium in sampling cell (12) and a gas-tight connection around the SPME fiber (21) by means of the gasket compression cap (13) to form the gasket compression, Sampling with x. thermal desorption (TD) tube (20) 30 In this case, in the gas outlet line of the thermal desorption (TD) tube (20), the gas coming out of the skin sampling cell (12) will pass through positioning in this way, 26 xi. In the case of static sampling, the pollutants present in the system After removal with inert gas, the gas inlet valve (18) and by closing the gas outlet valve (19) the skin the sampling cell (12) becomes a closed sampling volume the introduction and release of volatile organic compounds from the skin surface 5 in the skin sampling cell during the determined sampling period (12) accumulation, xii. In the gas phase of the skin sampling cell (12) during static sampling accumulated volatile organic compounds are adsorbed by SPME fiber (21) or absorption, 10 xiii. In case of dynamic sampling, the gas inlet valve (18) and keeping the gas outlet valve (19) in the open position and gas sampling skin sampling of inert gas by operating the pump (15) passing through the cell (12) at a continuous or specified flow rate xiv. Volatile organic compounds released from the skin surface during dynamic sampling 15 Contact of gas containing compounds with SPME fiber (21) or gas from the thermal desorption (TD) tube (20) located in the outlet line by passing these volatile organic compounds through SPME fibers (21) or collection on a thermal desorption (TD) tube (20), xv. After the completion of the specified sampling period, SPME 20 If fiber (21) is used, SPME fiber (21) from the device removal and inlet block (22) of the gas chromatography device (23) placement, xvi. Inlet of volatile organic compounds retained on SPME fiber (21) thermal desorption at a suitable temperature in block (22) and 25 analysis in gas chromatography device (23), xvii. In case a thermal desorption (TD) tube (20) is used Thermal desorption after the sampling process is complete. (TD) tube (20) is removed from the gas outlet line and gas Placement of the TD device (24) connected to the chromatography device (23), 30 xviii. Adsorbent material in thermal desorption (TD) tube (20) The volatile organic compounds retained on the TD device (24) are suitable desorb at temperature and into a gas chromatography device (23) by transferring and analyzing it, 27 19. After the sampling process is complete, the vacuum pump (16) stopping, removing the device from the skin surface and restarting disconnecting the device connections for use It includes the steps of the process. 10 20 30

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