A novel nucleic acid molecule with improved expression efficiency and sustainability and uses thereof

Abstract

The present invention relates to a modified mRNA molecule capable of efficiently expressing a target protein in vivo or in a biological sample. The RNA molecule of the present invention exhibits significantly improved expression level and expression duration of a target protein by inserting a virus-derived stem-loop α (SLα) structure or an analogue thereof at an appropriate position within the 3' UTR such that an optimal spatial distance from the poly(A) tail is maintained. Accordingly, the present invention may be advantageously used as an excellent nucleic acid therapeutic composition capable of stably and continuously producing therapeutically effective amounts of the target protein even at a low dosage.