Systems and methods for performing solid phase oligonucleotide synthesis

Abstract

Aspects disclosed herein relate to methods and systems for performing Solid Phase Oligonucleotide Synthesis (SPOS). In some embodiments, a system configured for use in SPOS may include an obstruction (e.g., a bar) that is configured to disrupt cohesion of a resin cake during SPOS to promote fluidization and mixing of the resin with a reagent. In some embodiments, a system configured for use in SPOS may include one or more spray devices (e.g., spray nozzles and / or spray balls), where the spray devices may be configured to provide solvents and / or reagents to the resin. The spray devices may also be configured to provide wash solvent to wash the walls of the reactor vessel.

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