Systems and methods for producing therapeutic proteins using single pass tangential flow filtration for in-line concentration and volume reduction of production pools

Abstract

The inventions provide improved systems and methods for inline concentrating and reducing intermediate process pools to mitigate plant fit challenges associated with high-titer protein purification process. The inventions also provide methods for concentrating and reducing VI pool volumes by integrating columns of single pass tangential flow filtration (SPTFF) system with viral inactivation pool filtration (VIPF) in protein purification process. Therapeutic proteins purified by the methods also are provided.

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