Improved microbial culture device

Abstract

The present invention provides an improved microbial culture device and related preparation methods, aiming to address the issues of complex operation and high infection risk in traditional culture devices. The device comprises a top cover, a porous carrier, a supplement tube, and a base. The top cover includes air vents, inoculation ports, and liquid supplement ports, equipped with an air filter and a detachable sealing component. The porous carrier is coated with a nutrient layer and features supplement holes, while the supplement tube connects the liquid supplement ports to the supplement holes. This integrated structure facilitates supplementation operations and reduces contamination risks. This invention also provides a method for preparing liquid fertilizer from Cinnamomum kanehirae fermentation, wherein branches and leaves of Cinnamomum kanehirae are fermented with saccharides to produce liquid fertilizer. Additionally, a method for manufacturing porous carriers is disclosed, which employs specific materials and techniques to create a high-porosity structure, enhancing the performance of the carrier.

Description

[Technical Field]

[0001] This disclosure relates to a bacterial culture device, and more particularly to an improved bacterial culture device. [Previous Technology]

[0002] In the prior art, there is a fungal culture device that can be used to cultivate Antrodia camphorata. The device is equipped with a sealing film on the top to achieve a sealing effect and provide a suitable environment for fungal growth.

[0003] However, the device has several inconveniences in actual use. For example, each time water, nutrient solution or air is added, the user needs to tear off the sealing film to operate. After the addition is completed, the residual adhesive on the sealing film needs to be thoroughly removed and a new sealing film needs to be applied. The operation steps are cumbersome and time-consuming.

[0004] Even worse, this device can usually only be used for a single culture of Antrodia camphorata. During the process of cleaning the residual adhesive from the sealing film, the inside of the device is easily contaminated, increasing the risk of contamination by other bacteria, thereby affecting the success rate of the culture and the quality of the bacteria.

[0005] In summary, due to the aforementioned inconvenience in operation and high risk of infection, the existing technology in the design and use of bacterial culture devices has limited application efficiency and economy, and also has an adverse effect on the stability of bacterial culture and the convenience of actual use. [Summary of the Invention]

[0006] The main purpose of this disclosure is to solve the problem of high infection risk that may occur when culturing Antrodia camphorata using conventional cell culture devices, each time water, nutrient solution or air is added, and the need to clean the residual adhesive of the sealing film.

[0007] To achieve the above objectives, this disclosure provides an embodiment of a bacterial culture device, comprising a top cover, a porous carrier, a replenishment tube, and a base. The top cover has at least one vent, one inoculation hole, and one replenishment hole. The vent is equipped with an air filter, and the inoculation hole is equipped with a detachable first closure. A replenishment hole is opened on the side of the porous carrier, and the surface of the porous carrier is covered with a nutrient layer. The two ends of the replenishment tube are a first end and a second end, respectively. The second end extends through the replenishment hole, and the first end extends through the replenishment hole. The first end is equipped with a detachable second closure. The base has an accommodating space for accommodating the porous carrier and the replenishment tube. The base can be detachably closed with the top cover.

[0008] In another embodiment of this disclosure, a method for preparing a fermented fertilizer solution of camphor tree is provided, comprising the following steps: (a) adding branches and leaves of camphor tree to water and sugars, wherein the ratio of water to sugars is 10:1 to 20:1, to form fermentation raw materials; (b) adding fermentation bacteria to the fermentation raw materials, maintaining a fermentation temperature of 30°C to 40°C, controlling the pH value at 5.5 to 7.0, and stirring and aerating regularly; (c) fermenting for up to 6 months, and then filtering to obtain the liquid fertilizer solution of camphor tree.

[0009] In another embodiment of this disclosure, a method for sintering a porous carrier is provided, comprising the following steps: (a) Preparation of ingredients and slurry: Alumina (Al₂O₃), silicon dioxide (SiO₂), and iron oxide (Fe₂O₃) are mixed, and then inorganic additives, binders, and foaming agents that enhance sintering properties and allow pores to form during the sintering process are added. Water is then added to prepare a slurry, which is ground to the required fineness and homogenized before use; (b) Granulation: The prepared slurry is dried and sieved using an atomizing dryer to form a powder material with a particle size of 10 to 150 micrometers (µm); (c) Aging treatment: The prepared powder material is placed in an environment with controlled temperature and humidity to ensure uniform distribution of moisture and stress within the material; (d) Heat treatment: The aged powder material is laid in a suitable carrier and subjected to heat treatment at a high temperature of 1200°C to 1400°C for at least 8 hours, during which the foaming agent decomposes and releases gas to form a porous structure; (e) Cooling and processing: The fired porous carrier is cooled at room temperature and cut, surface treated or processed as required to achieve the required specifications.

[0010] As described above, this disclosure achieves the purpose of repeated use without the need to clean residual adhesive through a detachable top cover and base, significantly improving ease of use. At the same time, the air filter installed through the air vents, along with the inoculation and replenishment holes that can be sealed at any time, effectively prevents the invasion of external bacteria, greatly reduces the risk of infection, and ensures the stability and success rate of bacterial culture.

[0011] Furthermore, the porous carrier's permeable structure and nutrient layer in the device provide a stable growth environment for the bacteria, further improving cultivation efficiency and quality. The preparation process of the porous carrier achieves a balance between high porosity and structural stability through strict control of raw material ratios and calcination processes, enhancing nutrient transfer efficiency and providing better support for bacterial growth.

[0012] Finally, this disclosure also provides a method for preparing camphor tree fermented fertilizer liquid, which utilizes camphor tree branches and leaves and sugars for fermentation to generate a high-nutrient and environmentally friendly liquid fertilizer, which not only improves the utilization rate of raw materials, but also expands the application value of camphor tree by-products.

Implementation Method

[0013] In order to make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments of this disclosure are described below in detail with reference to the accompanying drawings:

[0014] Referring to Figures 1 to 8, a bacterial culture device 100 is disclosed, comprising a top cover 10, a porous carrier 20, a replenishment tube 30, and a base 40. The top cover 10 has at least one vent 11, one inoculation hole 12, and one replenishment hole 13. The vent 11 is provided with an air filter 50, and the inoculation hole 12 is provided with a detachable first closure 51. The porous carrier 20 has a replenishment hole 21 on its side, and the surface of the porous carrier 20 is covered with a nutrient layer 60. The replenishment tube 30 has a first end 31 and a second end 32 at its two ends, respectively. The second end 32 extends through the replenishment hole 21, and the first end 31 extends through the replenishment hole 13. The first end 31 is provided with a detachable second closure 52. The base 40 has an accommodating space 41 for accommodating the porous carrier 20 and the replenishment tube 30. The base 40 can be detachably closed with the top cover 10.

[0015] With the above structure, the vents 11 on the top cover 10 are equipped with air filters 50, which can effectively promote gas exchange and prevent external pollutants from entering, maintaining a stable culture environment. The inoculation well 12 and the liquid replenishment well 13 are respectively equipped with detachable first sealing members 51 and second sealing members 52, providing flexible and safe inoculation and liquid replenishment operations, reducing the risk of contamination. The nutrient layer 60 on the surface of the porous carrier 20 can improve the culture efficiency, and its porous structure further promotes the transport of nutrients and gases.

[0016] Please refer to Figures 1 to 4. The top of the base 40 is provided with a plurality of positioning pieces 42. The upper cover 10 has a plurality of fasteners 14 corresponding to the plurality of positioning pieces 42. The fasteners 14 can be fastened and locked onto the corresponding positioning pieces 42 to form a tight fixing structure, ensuring that the upper cover 10 and the base 40 are firmly connected. Since the base 40 and the upper cover 10 are detachable, they can be reused to cultivate a new batch of mycelium or mushrooms. There is no need to deal with residual glue, which improves the convenience of use.

[0017] Please refer to Figures 2 to 4. The bottom of the upper cover 10 is provided with a groove 15, and a sealing ring 53 is provided in the groove 15. The base 40 has a flange 43 corresponding to the position of the groove 15. When the upper cover 10 is fastened to the base 40, the flange 43 presses against the sealing ring 53, so that the sealing ring 53 is elastically deformed to enhance the airtightness, effectively blocking the internal and external environment of the bacterial culture device 100 and preventing external pollutants from entering.

[0018] Please refer to Figures 2 and 3. A first fixing plug 54 is provided in the replenishment hole 13, and the first end 31 of the replenishment tube 30 can extend into the first fixing plug 54. A second fixing plug 55 is provided in the replenishment hole 21, and the second end 32 of the replenishment tube 30 can extend into the second fixing plug 55. Both the first fixing plug 54 and the second fixing plug 55 can enhance the stability and firmness of the replenishment tube 30 in connection with the upper cover 10 and the base 40. In addition, the replenishment tube 30 has a positioning groove 33 on the surface adjacent to the second end 32. The positioning groove 33 is used to indicate the insertion depth of the replenishment tube 30. When the second end 32 of the replenishment tube 30 is inserted and extends into the second fixing plug 55, when the positioning groove 33 is aligned with the edge of the second fixing plug 55, it indicates that the insertion depth of the replenishment tube 30 has reached the predetermined position, and further insertion of the replenishment tube 30 should be stopped.

[0019] Please continue to refer to Figures 2 and 3. A plurality of reinforcing threads 551 can also be provided around the surface of the second fixed plug 55. The reinforcing threads 551 can improve the bonding strength and stability between the second fixed plug 55 and the porous carrier 20.

[0020] Please refer to Figures 2 and 5. A carrier 56 is provided on each side of the accommodating space 41 of the base 40. Each carrier 56 has a liquid container 561, which can hold an inducing agent. Different types of inducing agents can be added as needed. Their function is to achieve specific effects by regulating the growth environment of fungi or mushrooms, such as stimulating the proliferation of fungi or mushrooms, or limiting the excessive growth of fungi or mushrooms, so as to precisely control the cultivation process and improve cultivation efficiency.

[0021] Please refer to Figures 2 and 5. The porous carrier 20 has a circumferential surface 22 and a plurality of micropores 23 recessed into the circumferential surface 22. The nutrient layer 60 is formed on the walls of the circumferential surface 22 and the plurality of micropores 23, ensuring that the fungi or mushrooms can obtain nutrients evenly and sufficiently, promoting their growth and reproduction. At the same time, the micropores 23 provide good channels for the flow of gas, liquid and nutrients, and greatly increase the effective contact area, improving the cultivation efficiency.

[0022] Please refer to Figures 2 and 5. The porous carrier 20 has a plurality of planting holes 24. The planting holes 24 are located at a position lower than the supplementary holes 21 on the porous carrier 20, and the supplementary holes 21 are not connected to the planting holes 24. The planting holes 24 can increase the surface area for the attachment and growth of fungi or mushrooms, thereby increasing the yield of a single batch. In addition, the non-connection design between the planting holes 24 and the supplementary holes 21 avoids the direct flow of the culture medium into the planting holes 24, reducing nutrient competition and maintaining good growth conditions for fungi or mushrooms. Since the supplementary holes 21 are located above the planting holes 24, nutrients can be naturally distributed into the interior of the porous carrier 20 under the action of gravity, ensuring that fungi or mushrooms can absorb nutrients evenly.

[0023] The nutrient layer 60 includes a fermentable carbon source accounting for 40% to 60% of the total weight of the nutrient layer 60, a nutrient supplement accounting for 5% to 15% of the total weight of the nutrient layer 60, a structural enhancer accounting for 5% to 10% of the total weight of the nutrient layer 60, a thickener accounting for 0.1% to 1% of the total weight of the nutrient layer 60, water accounting for 30% to 50% of the total weight of the nutrient layer 60, and 10 mL to 20 mL of camphor tree fermented fertilizer solution added annually.

[0024] The fermentable carbon source is selected from one or a combination of glucose, molasses, or brown sugar. The fermentable carbon source may be 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60% of the total weight of the nutrient layer 60.

[0025] The nutritional supplement is selected from one or a combination of malt extract and yeast. The nutritional supplement may be 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9% of the total weight of the nutrient layer. %, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 10.1%, 10.2%, 10.3%, 10.4%, 10.5%, 10.6%, 10.7%, 10.8%, 10.9%, 11%, 11.1%, 11.2%, 11.3%, 11.4%, 11.5%, 11.6%, 11.7%, 11.8%, 11.9%, 12%, 12. 1%, 12.2%, 12.3%, 12.4%, 12.5%, 12.6%, 12.7%, 12.8%, 12.9%, 13%, 13.1%, 13.2%, 13.3%, 13.4%, 13.5%, 13.6%, 13.7%, 13.8%, 13.9%, 14%, 14.1%, 14.2%, 14.3%, 14.4%, 14.5%, 14.6%, 14.7%, 14.8%, 14.9%, or 15%.

[0026] The structural reinforcing agent is selected from one or a combination of calcium lignosulfonate or chitin. The structural reinforcing agent may be 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, or 10% of the total weight of the nutrient layer.

[0027] The thickener is selected from egg white gel. The thickener may be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1% of the total weight of the nutrient layer.

[0028] Water may be 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49% or 50% of the total weight of the nutrient layer 60.

[0029] Camphor tree fermented fertilizer liquid can be 10 mL, 10.5 mL, 11 mL, 11.5 mL, 12 mL, 12.5 mL, 13 mL, 13.5 mL, 14 mL, 14.5 mL, 15 mL, 15.5 mL, 16 mL, 16.5 mL, 17 mL, 17.5 mL, 18 mL, 18.5 mL, 19 mL, 19.5 mL or 20 mL.

[0030] Through the above structure, a fermentable carbon source provides the main energy source for the microorganisms, promoting their growth and metabolism. Nutrient supplements provide the microorganisms with the necessary trace elements and vitamins, ensuring a balance of physiological functions. A structural enhancer strengthens the mechanical strength and stability of the nutrient layer 60, preventing damage during use. A thickener enhances the viscosity and uniformity of the nutrient layer 60, preventing component separation or sedimentation. Water provides appropriate humidity and a dissolving medium to support the physiological needs of the microorganisms. Furthermore, the annual addition of camphor tree fermented fertilizer, made from pure camphor tree branches and leaves using a liquid fertilizer fermentation process, contains natural active ingredients that further promote the activity and growth efficiency of the microorganisms. The synergistic effect of these components ensures the stability and high efficiency of the nutrient layer 60, providing optimized conditions for microbial cultivation.

[0031] Please refer to Figures 6 and 7a to 7c, along with Figures 1 to 5. The preparation method of the camphor tree fermented fertilizer liquid disclosed herein includes the following steps:

[0032] Step S1: Add the branches and leaves of the camphor tree to water and sugars in a ratio of 10:1 to 20:1 to form fermentation raw materials. Figure 7a is a schematic diagram of an example after fermentation for about 1 to 2 months. The ratio of water to sugars can be 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1 or 20:1.

[0033] Step S2: Add fermentation broth to the fermentation raw materials, maintain a fermentation temperature of 30°C to 40°C, control the pH value between 5.5 and 7.0, and stir and aerate regularly. The fermentation broth is one or a combination of lactic acid bacteria, yeast, and Bacillus subtilis. Figure 7b is a schematic diagram of an example after fermentation for about 2 to 4 months. The pH value can be 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, or 7.0.

[0034] Step S3: Fermentation for up to 6 months, followed by filtration, yields camphor tree liquid fertilizer. Figure 7c is a schematic diagram of an example after fermentation for 4 to 6 months.

[0035] Please refer to Figure 8 and, together with Figures 1 to 5, the firing method of the porous carrier 20 disclosed herein includes the following steps:

[0036] Step P1, Ingredient Preparation and Slurry Preparation: Aluminum oxide (Al₂O₃), silicon dioxide (SiO₂), and ferric oxide (Fe₂O₃) are mixed in a ratio of approximately 50:40:1. Inorganic additives, fluxes, binders, and foaming agents that create pores during the firing process are added to enhance sintering properties. Water is then added to prepare a slurry, which is ground to the required fineness and homogenized before use. The inorganic additives are one or a combination of calcium oxide (CaO), magnesium oxide (MgO), or titanium dioxide (TiO₂). The flux is one or a combination of potassium oxide (K₂O) or sodium oxide (Na₂O). The binder is one or a combination of clay, bentonite, kaolin, polyvinyl alcohol (PVA), or silicate (Na₂SiO₃). The foaming agent is one or a combination of calcium carbonate (CaCO₃), sodium bicarbonate (NaHCO₃), urea, or barium carbonate (BaCO₃).

[0037] Step P2, Granulation: The prepared slurry is dried and sieved using an atomizing dryer to form a powder material with a size of 10 to 150 micrometers (µm). The powder size is 10 micrometers, 20 micrometers, 30 micrometers, 40 micrometers, 50 micrometers, 60 micrometers, 70 micrometers, 80 micrometers, 90 micrometers, 100 micrometers, 110 micrometers, 120 micrometers, 130 micrometers, 140 micrometers, or 150 micrometers.

[0038] Step P3, Aging Treatment: The prepared powder material is placed in an environment with controlled temperature and humidity to ensure uniform distribution of moisture and stress within the material. The aging treatment temperature range is between 20℃ and 40℃, and the above temperature can be 20℃, 21℃, 22℃, 23℃, 24℃, 25℃, 26℃, 27℃, 28℃, 29℃, 30℃, 31℃, 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, 39℃, or 40℃. The aging treatment humidity range is between 50% and 75% relative humidity (RH), and the above humidity can be 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, or 75%.

[0039] Step P4, heat treatment: The aged powder material is laid in a high-temperature resistant carrier and subjected to heat treatment at a high temperature of 1200°C to 1400°C for at least 8 hours, during which the foaming agent decomposes and releases gas to form a porous structure. After the above-mentioned high-temperature treatment at 1200°C to 1400°C, chemical pollution and heavy metal pollution will be reduced to extremely low levels or even disappear. The high temperature can be 1200°C, 1210°C, 1220°C, 1230°C, 1240°C, 1250°C, 1260°C, 1270°C, 1280°C, 1290°C, 1300°C, 1310°C, 1320°C, 1330°C, 1340°C, 1350°C, 1360°C, 1370°C, 1380°C, 1390°C or 1400°C.

[0040] Step P5, Cooling and processing: The fired porous carrier 20 is cooled at room temperature and then cut, surface treated or processed as needed to achieve the required specifications.

[0041] The advantages of this disclosure are as follows:

[0042] 1. Stable environment for cultured bacteria: After the top cover 10 is combined with the base 40, the gas input will be filtered through the air filter 50. The inoculation hole 12 and the liquid replenishment hole 13 are respectively equipped with a detachable first sealing member 51 and a second sealing member 52, which will be sealed after inoculation or liquid replenishment, effectively preventing external pollutants from entering and providing healthy growth conditions for fungi or mushrooms.

[0043] 2. Enhanced culture efficiency and yield: The combination of the porous carrier 20 structure and the nutrient layer 60 provides a sufficient and uniform nutrient supply; the design of the planting hole 24 and the micropore 23 further increases the attachment and growth area of ​​the bacteria, significantly improving the culture efficiency and yield of a single batch.

[0044] 3. Easy to reuse: The top cover 10 and the base 40 are detachable, so there is no need to tear off the seal or deal with the residual glue. The top cover 10 and the base 40 can be airtightly connected by a buckle.

[0045] Finally, it should be understood that the embodiments described in this disclosure are merely illustrative of the principles of the embodiments disclosed herein. Other variations may also fall within the scope of this disclosure. Therefore, alternative configurations of the embodiments disclosed herein are considered as examples and not limitations, and are regarded as consistent with the teachings of this disclosure. Accordingly, the embodiments of this disclosure are not limited to those explicitly described and illustrated herein. [Simplified Explanation of the Diagram]

[0046] Figure 1 is a three-dimensional schematic diagram of the microbial culture device disclosed herein. Figure 2 is a three-dimensional exploded schematic diagram of the microbial culture device disclosed herein. Figure 3 is a cross-sectional schematic diagram taken along section AA of Figure 1. Figure 4 is a partial enlarged schematic diagram of the structure in Figure 3. Figure 5 is a schematic diagram of an embodiment of adding air and nutrient solution to Antrodia camphorata in the microbial culture device disclosed herein. Figure 6 is a flowchart of the preparation method of Antrodia camphorata fermented fertilizer liquid disclosed herein. Figure 7a is a schematic diagram of an embodiment of adding Antrodia camphorata branches and leaves to water and sugars during the preparation of Antrodia camphorata fermented fertilizer liquid disclosed herein. Figure 7b is a schematic diagram of an embodiment of maintaining appropriate temperature and pH value and periodically stirring and aerating during the preparation of Antrodia camphorata fermented fertilizer liquid disclosed herein. Figure 7c is a schematic diagram of an embodiment of Antrodia camphorata fermented fertilizer liquid disclosed herein after fermentation during preparation. Figure 8 is a flowchart of the firing method of the porous carrier disclosed herein.

Claims

1. A bacterial culture device, comprising: a top cover having at least one vent, one inoculation hole, and one replenishment hole, wherein the vent is provided with an air filter, and the inoculation hole is provided with a detachable first closure; a porous carrier having a replenishment hole on its side, the surface of the porous carrier being covered with a nutrient layer; a replenishment tube having a first end and a second end at its two ends, the second end extending through the replenishment hole, the first end extending through the replenishment hole, and the first end being provided with a detachable second closure; and a base having an accommodating space for accommodating the porous carrier and the replenishment tube, the base being detachably closed with the top cover.

2. The bacterial culture apparatus as described in claim 1, wherein, The top of the base is provided with a plurality of positioning pieces, and the top cover has a plurality of fasteners corresponding to the plurality of positioning pieces, which can be fastened and locked onto the corresponding plurality of positioning pieces.

3. The bacterial culture apparatus as described in claim 1, wherein, The bottom of the top cover is provided with a groove and a sealing ring is provided in the groove. The base has a flange corresponding to the groove. When the top cover is fastened to the base, the flange presses against the sealing ring.

4. The bacterial culture apparatus as described in claim 1, wherein, The fluid replenishment hole is provided with a first fixing plug, and the first end of the replenishment tube can extend to the first fixing plug.

5. The bacterial culture apparatus as described in claim 1, wherein, The supplementary hole is provided with a second fixing plug, and the second end of the supplementary tube can extend to the second fixing plug.

6. The bacterial culture apparatus as described in claim 5, wherein, The surface of the second fixed plug is provided with multiple reinforcing threads.

7. The bacterial culture apparatus as described in claim 1, wherein, The supplementary tube has a positioning groove on the surface adjacent to the second end, which is used to indicate the insertion depth of the supplementary tube.

8. The bacterial culture apparatus as described in claim 1, wherein, The base has a carrier on each side of the accommodating space, and each carrier has a liquid tank.

9. The bacterial culture apparatus as described in claim 1, wherein, The porous carrier has a circumferential surface and a plurality of micropores recessed into the circumferential surface, and the nutrient layer is formed on the circumferential surface and the walls of the plurality of micropores.

10. The bacterial culture apparatus as described in claim 9, wherein, The porous carrier has a plurality of planting holes, which are located at a position relatively lower than the supplementary hole, and the supplementary hole is not connected to the plurality of planting holes.

11. The bacterial culture apparatus as described in claim 1, wherein, The nutrient layer includes 40% to 60% of the total weight of the nutrient layer, 5% to 15% of the total weight of the nutrient layer, 5% to 10% of the total weight of the nutrient layer, 0.1% to 1% of the total weight of the nutrient layer, 30% to 50% of the total weight of the nutrient layer, and 10 mL to 20 mL of camphor tree fermented fertilizer solution added annually.

12. The bacterial culture apparatus as described in claim 11, wherein, The fermentable carbon source is selected from one or a combination of glucose, molasses, or brown sugar.

13. The bacterial culture apparatus as described in claim 11, wherein, The nutritional supplement is selected from one or a combination of malt extract or yeast.

14. The bacterial culture apparatus as described in claim 11, wherein, The structural reinforcing agent is selected from one or a combination of calcium lignosulfonate or chitin.

15. The bacterial culture apparatus as described in claim 11, wherein, The thickener is selected from egg white gel.

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