Method for preparing liquid fertilizer from cinnamomum kanehirae fermentation

Abstract

The present invention provides a method for preparing liquid fertilizer from Cinnamomum kanehirae fermentation. In this method, branches and leaves of Cinnamomum kanehirae are fermented with saccharides to produce a liquid fertilizer. The resulting product is a high-nutrient and environmentally friendly fertilizer that not only enhances raw material utilization but also increases the value-added applications of Cinnamomum kanehirae by-products.

Description

Technical Field

[0001] This disclosure discloses a method for preparing fermented fertilizer liquid, particularly a method for preparing fermented fertilizer liquid from camphor tree. Prior Technology

[0002] In the prior art, there is a fungal culture device that can be used to cultivate Antrodia camphorata. The device has a sealing film on the top to achieve a sealing effect and provide a suitable environment for fungal growth.

[0003] However, the device has several inconveniences in actual use. For example, each time water, nutrient solution or air is added, the user has to tear off the sealing film to operate, and after the addition is completed, the residual adhesive on the sealing film must be thoroughly removed and a new sealing film must be applied. The operation steps are cumbersome and time-consuming.

[0004] Even worse, this device can usually only be used for a single cultivation of Antrodia camphorata. During the process of cleaning the residual adhesive from the sealing film, the inside of the device is easily contaminated, increasing the risk of contamination by other bacteria, which in turn affects the success rate of cultivation and the quality of the bacteria.

[0005] In summary, existing technologies for the design and use of bacterial culture devices suffer from drawbacks such as inconvenient operation and high risk of infection, which limit their application efficiency and economy, and also adversely affect the stability of bacterial culture and the convenience of actual use. Summary of the Invention

[0006] The main purpose of this disclosure is to address the high risk of infection that may arise each time water, nutrient solution, or air is added when culturing Antrodia camphorata using conventional cell culture devices, as well as the need to clean residual adhesive from the sealing film.

[0007] To achieve the above objectives, this disclosure provides an embodiment of a bacterial culture device, comprising a top cover, a porous carrier, a replenishment tube, and a base. The top cover has at least one vent, one inoculation hole, and one replenishment hole. The vent is equipped with an air filter, and the inoculation hole has a detachable first closure. The porous carrier has a replenishment hole on its side, and its surface is covered with a nutrient layer. The replenishment tube has a first end and a second end at its two ends, the second end extending through the replenishment hole and the first end extending through the replenishment hole, and the first end has a detachable second closure. The base has an accommodating space for accommodating the porous carrier and the replenishment tube, and the base can be detachably closed with the top cover.

[0008] In another embodiment of this disclosure, a method for preparing a fermented fertilizer solution of camphor tree is provided, comprising the following steps: (a) adding branches and leaves of camphor tree to water and sugars in a ratio of 10:1 to 20:1 to form fermentation raw materials; (b) adding fermentation bacteria to the fermentation raw materials, maintaining a fermentation temperature of 30°C to 40°C, controlling the pH value at 5.5 to 7.0, and stirring and aerating regularly; (c) fermenting for up to 6 months, followed by filtration to obtain the liquid fertilizer solution of camphor tree.

[0009] In another embodiment of this disclosure, a method for sintering a porous carrier is provided, comprising the following steps: (a) Preparation of ingredients and slurry: Alumina (Al₂O₃), silicon dioxide (SiO₂), and iron oxide (Fe₂O₃) are mixed, and then inorganic additives, binders, and foaming agents that enhance sintering properties and allow pores to form during the sintering process are added. Water is then added to prepare a slurry, which is ground to the required fineness and homogenized before use; (b) Granulation: The prepared slurry is dried and sieved using an atomizing dryer to form a powder material with a particle size of 10 to 150 micrometers (µm); (c) Aging treatment: The prepared powder material is placed in an environment with controlled temperature and humidity to ensure uniform distribution of moisture and stress within the material; (d) Heat treatment: The aged powder material is laid in a suitable carrier and subjected to heat treatment at a high temperature of 1200°C to 1400°C for at least 8 hours, during which the foaming agent decomposes and releases gas to form a porous structure; (e) Cooling and processing: The fired porous carrier is cooled at room temperature and cut, surface treated or processed as required to achieve the required specifications.

[0010] Through the above, this disclosure demonstrates that the detachable top cover combined with the base allows for repeated use without the need to clean residual adhesive, significantly improving ease of use. Simultaneously, the inclusion of an air filter via vents, along with easily closable inoculation and replenishment holes, effectively prevents the invasion of external bacteria, greatly reducing the risk of infection and ensuring the stability and success rate of bacterial culture.

[0011] Furthermore, the porous carrier's permeable structure and nutrient layer provide a stable growth environment for the bacteria, further improving cultivation efficiency and quality. The preparation process of the porous carrier achieves a balance between high porosity and structural stability through strict control of raw material ratios and calcination techniques, enhancing nutrient transfer efficiency and providing better support for bacterial growth.

[0012] Finally, this disclosure also provides a method for preparing fermented cinnamon tree fertilizer, which utilizes the branches, leaves and sugars of cinnamon tree for fermentation to generate a high-nutrient and environmentally friendly liquid fertilizer, which not only improves the utilization rate of raw materials, but also expands the application value of cinnamon tree by-products. Simple Explanation of the Diagram

[0013] Figure 1 is a three-dimensional schematic diagram of the bacterial culture device disclosed herein. Figure 2 is a three-dimensional exploded view of the bacterial culture device disclosed herein. Figure 3 is a schematic cross-section taken along section line AA in Figure 1. Figure 4 is an enlarged schematic diagram of part of the structure in Figure 3. Figure 5 is a schematic diagram of an embodiment of adding air and nutrient solution to Antrodia camphorata in a cell culture device disclosed herein. Figure 6 is a flowchart of the preparation method of the camphor tree fermentation fertilizer liquid disclosed in this paper. Figure 7a is a schematic diagram of an example of preparing the fermented fertilizer liquid of camphor tree by adding branches and leaves of camphor tree to water and sugars. Figure 7b is a schematic diagram of an example of the preparation of the camphor tree fermented fertilizer solution disclosed herein, in which appropriate temperature and pH values ​​are maintained and the solution is stirred and aerated regularly. Figure 7c is a schematic diagram of an example of the fermented fertilizer solution of camphor tree disclosed in this paper after fermentation. Figure 8 is a flowchart of the firing method for the porous carrier disclosed in this paper. Implementation

[0014] To make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments of this disclosure are described below in detail with reference to the accompanying drawings:

[0015] Referring to Figures 1 to 8, a bacterial culture device 100 is disclosed, comprising a top cover 10, a porous carrier 20, a replenishment tube 30, and a base 40. The top cover 10 has at least one vent 11, one inoculation hole 12, and one replenishment hole 13. The vent 11 is equipped with an air filter 50, and the inoculation hole 12 is equipped with a detachable first closure 51. The porous carrier 20 has a replenishment hole 21 on its side, and a nutrient layer 60 is applied to its surface. The replenishment tube 30 has a first end 31 and a second end 32 at its two ends. The second end 32 extends through the replenishment hole 21, and the first end 31 extends through the replenishment hole 13. The first end 31 is equipped with a detachable second closure 52. The base 40 has a receiving space 41 for accommodating the porous carrier 20 and the replenishment tube 30. The base 40 can be detachably closed with the top cover 10.

[0016] With the above structure, the vents 11 on the top cover 10 are equipped with air filters 50, which can effectively promote gas exchange and prevent external contaminants from entering, maintaining a stable culture environment. The inoculation well 12 and the liquid replenishment well 13 are respectively equipped with detachable first sealing members 51 and second sealing members 52, providing flexible and safe inoculation and liquid replenishment operations, reducing the risk of contamination. The nutrient layer 60 on the surface of the porous carrier 20 can improve culture efficiency, and its porous structure further promotes the transport of nutrients and gases.

[0017] Please refer to Figures 1 to 4. The top of the base 40 is provided with a plurality of positioning pieces 42. The upper cover 10 has a plurality of fasteners 14 corresponding to the plurality of positioning pieces 42. The fasteners 14 can be fastened and locked onto the corresponding positioning pieces 42 to form a tight fixing structure, ensuring that the upper cover 10 and the base 40 are firmly connected. Since the base 40 and the upper cover 10 are detachable, they can be reused to cultivate a new batch of mycelium or mushrooms, eliminating the need to deal with residual glue and improving the convenience of use.

[0018] Please refer to Figures 2 to 4. The bottom of the upper cover 10 is provided with a groove 15, and a sealing ring 53 is provided in the groove 15. The base 40 has a flange 43 corresponding to the position of the groove 15. When the upper cover 10 is fastened to the base 40, the flange 43 presses against the sealing ring 53, so that the sealing ring 53 is elastically deformed to enhance the airtightness, effectively blocking the internal and external environment of the bacterial culture device 100 and preventing external pollutants from entering.

[0019] Please refer to Figures 2 and 3. A first fixing plug 54 is provided in the replenishment hole 13, through which the first end 31 of the replenishment tube 30 can extend. A second fixing plug 55 is provided in the replenishment hole 21, through which the second end 32 of the replenishment tube 30 can extend. Both the first fixing plug 54 and the second fixing plug 55 enhance the stability and firmness of the replenishment tube 30 in connection with the upper cover 10 and the base 40. Furthermore, the replenishment tube 30 has a positioning groove 33 on the surface adjacent to the second end 32. The positioning groove 33 indicates the insertion depth of the replenishment tube 30. When the second end 32 of the replenishment tube 30 is inserted and extends through the second fixing plug 55, and the positioning groove 33 aligns with the edge of the second fixing plug 55, it indicates that the insertion depth of the replenishment tube 30 has reached the predetermined position, and further insertion of the replenishment tube 30 should be stopped.

[0020] Please refer to Figures 2 and 3. Alternatively, a plurality of reinforcing threads 551 can be provided around the surface of the second fixing plug 55. The reinforcing threads 551 can improve the bonding strength and stability between the second fixing plug 55 and the porous carrier 20.

[0021] Please refer to Figures 2 and 5. A carrier 56 is provided on each side of the accommodating space 41 of the base 40. Each carrier 56 has a liquid container 561, which can hold an inducing agent. Different types of inducing agents can be added as needed. Their function is to achieve specific effects by regulating the growth environment of fungi or mushrooms, such as stimulating the proliferation of fungi or mushrooms, or limiting their excessive growth, so as to precisely control the cultivation process and improve cultivation efficiency.

[0022] Please refer to Figures 2 and 5. The porous carrier 20 has a circumferential surface 22 and a plurality of micropores 23 recessed into the circumferential surface 22. The nutrient layer 60 is formed on the walls of the circumferential surface 22 and the plurality of micropores 23, ensuring that the fungi or mushrooms can obtain nutrients evenly and sufficiently, promoting their growth and reproduction. At the same time, the micropores 23 provide good channels for the flow of gas, liquid and nutrients, and greatly increase the effective contact area, improving the cultivation efficiency.

[0023] Please refer to Figures 2 and 5. The porous carrier 20 has a plurality of planting holes 24. These planting holes 24 are positioned lower than the supplementary holes 21 within the porous carrier 20, and the supplementary holes 21 are not connected to the planting holes 24. The planting holes 24 increase the surface area for fungi or mushrooms to attach and grow, thus increasing the yield per batch. Furthermore, the non-connection between the planting holes 24 and the supplementary holes 21 prevents the culture medium from directly flowing into the planting holes 24, reducing nutrient competition and maintaining favorable growth conditions for the fungi or mushrooms. Since the supplementary holes 21 are located above the planting holes 24, nutrients are naturally distributed into the porous carrier 20 under gravity, ensuring that the fungi or mushrooms can absorb nutrients evenly.

[0024] The nutrient layer 60 includes 40% to 60% of the total weight of the nutrient layer 60, 5% to 15% of the total weight of the nutrient layer 60, 5% to 10% of the total weight of the nutrient layer 60, 0.1% to 1% of the total weight of the nutrient layer 60, 30% to 50% of the total weight of the nutrient layer 60, and 10 mL to 20 mL of camphor tree fermented fertilizer solution added annually.

[0025] The fermentable carbon source is selected from one or a combination of glucose, molasses, or brown sugar. The fermentable carbon source may be 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60% of the total weight of the nutrient layer.

[0026] The nutritional supplement is selected from one or a combination of malt extract and yeast. The nutritional supplement may constitute 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9% of the total weight of the nutrient layer. %, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 10.1%, 10.2%, 10.3%, 10.4%, 10.5%, 10.6%, 10.7%, 10.8%, 10.9%, 11%, 11.1%, 11.2%, 11.3%, 11.4%, 11.5%, 11.6%, 11.7%, 11.8%, 11.9%, 12%, 12. 1%, 12.2%, 12.3%, 12.4%, 12.5%, 12.6%, 12.7%, 12.8%, 12.9%, 13%, 13.1%, 13.2%, 13.3%, 13.4%, 13.5%, 13.6%, 13.7%, 13.8%, 13.9%, 14%, 14.1%, 14.2%, 14.3%, 14.4%, 14.5%, 14.6%, 14.7%, 14.8%, 14.9%, or 15%.

[0027] The structural reinforcing agent is selected from one or a combination of calcium lignosulfonate or chitin. The structural reinforcing agent may be 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.6%, 7.9%, 8%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, or 10% of the total weight of the nutrient layer.

[0028] The thickener is selected from egg white gel. The thickener may be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1% of the total weight of the nutrient layer.

[0029] Water can be 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, or 50% of the total weight of the nutrient layer.

[0030] Cinnamomum camphora fermented fertilizer liquid can be 10 mL, 10.5 mL, 11 mL, 11.5 mL, 12 mL, 12.5 mL, 13 mL, 13.5 mL, 14 mL, 14.5 mL, 15 mL, 15.5 mL, 16 mL, 16.5 mL, 17 mL, 17.5 mL, 18 mL, 18.5 mL, 19 mL, 19.5 mL or 20 mL.

[0031] Through the above structure, a fermentable carbon source provides the main energy source for the microorganisms, promoting their growth and metabolism. Nutrient supplements provide the microorganisms with the necessary trace elements and vitamins, ensuring a balance of physiological functions. A structural enhancer strengthens the mechanical strength and stability of the nutrient layer 60, preventing damage during use. A thickener enhances the viscosity and uniformity of the nutrient layer 60, preventing component separation or sedimentation. Water provides appropriate humidity and a dissolving medium, supporting the physiological needs of the microorganisms. Furthermore, the annual addition of fermented camphor tree fertilizer, made from pure camphor tree branches and leaves using a liquid fertilizer fermentation process, contains natural active ingredients that further promote the activity and growth efficiency of the microorganisms. The synergistic effect of these components ensures the stability and high efficiency of the nutrient layer 60, providing optimized conditions for microbial cultivation.

[0032] Please refer to Figures 6 and 7a to 7c, along with Figures 1 to 5. The preparation method of the camphor tree fermented fertilizer solution disclosed herein includes the following steps:

[0033] Step S1: Add the branches and leaves of the camphor tree to water and sugars in a ratio of 10:1 to 20:1 to form fermentation raw materials. Figure 7a is a schematic diagram of an example after fermentation for about 1 to 2 months. The water-to-sugar ratio can be 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, or 20:1.

[0034] Step S2: Add fermentation broth to the fermentation raw materials, maintain a fermentation temperature of 30°C to 40°C, control the pH value between 5.5 and 7.0, and stir and aerate regularly. The fermentation broth is one or a combination of lactic acid bacteria, yeast, and Bacillus subtilis. Figure 7b is a schematic diagram of an example after fermentation for 2 to 4 months. The pH value can be 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, or 7.0.

[0035] Step S3: Fermentation for up to 6 months, followed by filtration, yields camphor tree liquid fertilizer. Figure 7c is a schematic diagram of an example after fermentation for approximately 4 to 6 months.

[0036] Please refer to Figure 8 and, in conjunction with Figures 1 to 5, the firing method for a porous carrier 20 disclosed herein comprises the following steps:

[0037] Step P1, Ingredient Preparation and Slurry Preparation: Aluminum oxide (Al₂O₃), silicon dioxide (SiO₂), and ferric oxide (Fe₂O₃) are mixed in a ratio of approximately 50:40:1. Inorganic additives to enhance sintering properties, fluxes, binders, and foaming agents to create pores during the firing process are added. Water is then added to prepare a slurry, which is ground to the required fineness and homogenized before use. The inorganic additives are one or a combination of calcium oxide (CaO), magnesium oxide (MgO), or titanium dioxide (TiO₂). The flux is one or a combination of potassium oxide (K₂O) or sodium oxide (Na₂O). The binder is one or a combination of clay, bentonite, kaolin, polyvinyl alcohol (PVA), or silicate (Na₂SiO₃). The foaming agent is one or a combination of calcium carbonate (CaCO₃), sodium bicarbonate (NaHCO₃), urea, or barium carbonate (BaCO₃).

[0038] Step P2, Granulation: The prepared slurry is dried and sieved using an atomizing dryer to form powder material with a size of 10 to 150 micrometers (µm). The powder size is 10 micrometers, 20 micrometers, 30 micrometers, 40 micrometers, 50 micrometers, 60 micrometers, 70 micrometers, 80 micrometers, 90 micrometers, 100 micrometers, 110 micrometers, 120 micrometers, 130 micrometers, 140 micrometers, or 150 micrometers.

[0039] Step P3, Aging Treatment: The prepared powder material is placed in an environment with controlled temperature and humidity to ensure uniform distribution of moisture and stress within the material. The aging temperature range is between 20℃ and 40℃, and the specific temperatures can be 20℃, 21℃, 22℃, 23℃, 24℃, 25℃, 26℃, 27℃, 28℃, 29℃, 30℃, 31℃, 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, 39℃, or 40℃. The aging humidity range is between 50% and 75% relative humidity (RH), and the specific humidity values ​​can be 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, or 75%.

[0040] Step P4, Heat Treatment: The aged powder material is laid in a high-temperature resistant carrier and subjected to heat treatment at a high temperature of 1200°C to 1400°C for at least 8 hours, during which the foaming agent decomposes and releases gas to form a porous structure. After the above-mentioned high-temperature treatment at 1200°C to 1400°C, chemical pollution and heavy metal pollution will be reduced to extremely low levels or even disappear. The high temperature can be 1200°C, 1210°C, 1220°C, 1230°C, 1240°C, 1250°C, 1260°C, 1270°C, 1280°C, 1290°C, 1300°C, 1310°C, 1320°C, 1330°C, 1340°C, 1350°C, 1360°C, 1370°C, 1380°C, 1390°C or 1400°C.

[0041] Step P5, post-cooling processing: The fired porous carrier 20 is cooled at room temperature and then cut, surface treated or processed as needed to achieve the required specifications.

[0042] With the above structure, the advantages of this disclosure are:

[0043] 1. Stable environment for mycelial culture: After the top cover 10 is combined with the base 40, the gas input will be filtered through the air filter 50. The mycelial planting hole 12 and the liquid replenishment hole 13 are respectively equipped with a detachable first sealing part 51 and a second sealing part 52, which will be sealed after mycelial planting or liquid replenishment, effectively preventing external pollutants from entering and providing healthy growth conditions for fungi or mushrooms.

[0044] 2. Enhanced cultivation efficiency and yield: The combination of the porous carrier 20 structure and the nutrient layer 60 provides a sufficient and uniform nutrient supply; the design of planting holes 24 and micropores 23 further increases the attachment and growth area of ​​the cells, significantly improving the cultivation efficiency and yield of a single batch.

[0045] 3. Convenient for repeated use: The top cover 10 and the base 40 are detachable, eliminating the need to tear off the sealing film or deal with residual adhesive. The top cover 10 and the base 40 can be airtightly connected by a snap-fit ​​mechanism.

[0046] Finally, it should be understood that the embodiments described in this disclosure are merely illustrative of the principles of the embodiments disclosed herein. Other variations may also fall within the scope of this disclosure. Therefore, alternative configurations of the embodiments disclosed herein are considered as examples and not limitations, and are regarded as consistent with the teachings of this disclosure. Accordingly, the embodiments of this disclosure are not limited to those explicitly described and illustrated herein.

[0047] 100: Bacterial Culture Device 10: Top Cover 11: Stomata 12:Plant hole 13: Liquid replenishment hole 14: Backing film 15: Groove 20: Porous carrier 21: Supplementary Hole 22: Circumference 23: Micropores 24: Implant hole 30: Replenishment tube 31: First end 32: Second end 33: Positioning groove 40: Base 41: Storage space 42: Positioning Piece 43: Flange 50: Air Filter 51: First sealing element 52: Second closure 53: Sealing ring 54: First fixing plug 55: Second fixing plug 551: Reinforced thread 56: Seat 561: Liquid tank 60: Nutrient Layer S1~S3: Steps P1~P5: Steps

Claims

1. A method for preparing a fermented fertilizer solution of Cinnamomum camphora, comprising the following steps: (a) adding water and sugars to the branches and leaves of Cinnamomum camphora, wherein the ratio of water to sugars is 10:1 to 20:1, to form fermentation raw materials; (b) adding fermentation bacteria to the fermentation raw materials, maintaining a fermentation temperature of 30°C to 40°C, controlling the pH value at 5.5 to 7.0, and stirring and aerating regularly; (c) fermenting for up to 6 months, and then filtering to obtain the liquid fertilizer solution of Cinnamomum camphora.

2. The method for preparing the camphor tree fermented fertilizer solution as described in claim 1, wherein, The fermentation broth is one or a combination of lactic acid bacteria, yeast, and Bacillus subtilis.

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