Partitioning anion exchange chromatography for purification of recombinant adeno-associated virus (RAAV)

Abstract

The present inventions provide methods for purifying adeno-associated viruses (AAV) that comprise gene of interest (GOI). The present inventions provide partitioning anion-exchange chromatography (AEX), leveraging surface charge differences between empty and full capsids to create an environment of on-column competition for binding sites. This results in empty capsids being removed during the column loading phase, allowing the bulk of full capsids to be eluted in a concentrated, highly enriched pool. The inventions also provide methods for conducting partitioning anion-exchange chromatography in rapid cycling mode for AAV purification process intensification.

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