Immunoassay method and immunoassay device

Abstract

The present invention provides an immunoassay method and an immunoassay device capable of quantifying an assay subject in a sample even at a low concentration. The immunoassay method includes a step of irradiating a container or substrate containing a sample containing the assay subject and a plurality of labeled particles carrying antibodies capable of binding directly or indirectly to the assay subject with light after the sample containing the assay subject has contacted with the plurality of labeled particles carrying antibodies capable of binding directly or indirectly to the assay subject, wherein the light is irradiated into a region of the container or substrate containing the labeled particles, and a step of detecting the irradiated light that has passed through the region of the container or substrate containing the labeled particles, wherein the wavelength of the irradiated light is a wavelength that causes Mie scattering on each of the plurality of labeled particles.

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