Eukaryotic cell media

Abstract

This disclosure relates to eukaryotic cell culture media useful in the production of recombinant proteins from cultured cell lines. The eukaryotic cell culture media comprise integrated stress response inhibitor (ISRIB), and / or hypotaurine and / or increased level of iron, and / or suramin. The disclosure also provides methods using said eukaryotic cell culture media, e.g. for culturing mammalian cells with enhanced product titer, and / or growth,and / or viability and / or for producing a recombinant protein of comparable or better product quality by culturing a eukaryotic cell line that comprises a polynucleotide encoding the recombinant protein in said media under conditions suitable for production of the polypeptide. Also provided are use of the eukaryotic cell culture media for the production of a recombinant protein from a cultured cell line comprising a polynucleotide encoding the recombinant protein.

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