Methods for middle down antibody characterization

Abstract

This disclosure relates to new methods for antibody characterization sequencing, such as middle down antibody characterization and sequencing, for example, for de novo antibody sequencing, identifying known antibodies in a sample, or verifying the sequence of antibodies in a sample.In some embodiments, the methods involve exposing antibodies to cathepsin D, cathepsin L, and / or cathepsin D and L, followed by mass spectrometry and sequence identification and deconvolution.

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