Methods for manufacturing protein / nucleic acid complexes, groups of protein / nucleic acid complexes, and protein / nucleic acid complexes

Abstract

This invention provides a method for manufacturing a protein / nucleic acid complex comprising a capsid protein from adeno-associated virus and a genomic nucleic acid having at least one inverted terminal repeat sequence, and having infectivity in mammals. The genomic nucleic acid further comprises a target nucleic acid encoding a target protein. The method includes: (a-1) providing a first nucleic acid composed of a linear single-stranded nucleic acid, a linear double-stranded nucleic acid, or a circular single-stranded nucleic acid, comprising at least one inverted terminal repeat sequence and a target nucleic acid encoding a target protein; (a-2) providing a first nucleic acid comprising at least one inverted terminal repeat sequence and a target nucleic acid encoding a target protein; and (b) contacting the first protein and the first nucleic acid in a solvent to form the protein / nucleic acid complex.

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