Electroporation systems and methods for cargo loading into extracellular vesicles

Abstract

Described herein are electroporation systems and methods for cargo loading into extracellular vesicles. For example, according to one embodiment, there is a flow electroporation system, including: one or more fluid channels configured to receive one or more EV fluid streams; an EV stream inlet for the one or more EV fluid channels; an EV stream flow source fluidly coupled to the sample inlet; a cargo inlet downstream of an electric field region adjacent to the one or more fluid channels; a cargo flow source fluidly coupled to the cargo inlet; one or more cargo flow streams contain a cargo buffer with cargo molecules; at least one pump configured to deliver the one or more EV fluid streams and the one or more cargo flow streams into the one or more fluid channels; and a plurality of electrodes configured to deliver electroporation energy to the one or more EV fluid streams.

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