PPR protein editing modification methods, modified proteins, and their applications.

Abstract

The objective of this invention is to modify PPR editing proteins with C-to-U activity to promote RNA editing and translation of target RNA. The method for modifying a PPR editing protein with a C-terminal domain according to this invention includes the following steps a or b: a. replacing at least one of position 3 of the E1 motif, position 3 of the E2 motif, and position 1 of the PG domain in the C-terminal domain comprising the P2 motif, L2 motif, S2 motif, E1 motif, E2 motif, and PG domain; or b. replacing at least one of position 3 of the P2 motif, position 10 of the L2 motif, position 1 of the E1 motif, and position 13 of the E2 motif in the C-terminal domain comprising the P2 motif, L2 motif, S2 motif, E1 motif, E2 motif, and WW domain.

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