Raman detecting method and evaluating method using the same

Abstract

The present invention relates to a Raman detecting method, including the following steps: providing a Raman detecting substrate, wherein the Raman detecting substrate includes a substrate, a hydrophobic layer and a hydrophilic layer. The hydrophobic layer is disposed on the substrate, the hydrophilic layer is disposed on the hydrophobic layer, and the hydrophobic layer is disposed surround the hydrophilic layer; dropping a sample liquid onto the hydrophilic layer of the Raman detecting substrate to form a microdroplet lens; illuminating the microdroplet lens by a Raman excitation light generated by a Raman spectrometer to obtain a Raman spectrum. Moreover, the present invention also relates to an evaluating method using the aforementioned Raman detecting method.

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