Using pineapple peel to increase bacterial cellulose production and applying it to the preparation of bio-leather

Abstract

This invention provides a method for manufacturing bacterial cellulose, comprising: adding a buffer solution to a homogenized pineapple peel as the main carbon source, adding a lignoacetic acid bacteria strain for culture to obtain bacterial cellulose, and then performing an emulsion polymerization reaction with an AESO emulsion to obtain an emulsion-modified bacterial cellulose product; this product has better yield, crystallinity, heat resistance and tensile strength than cellulose obtained from other carbon source culture media, and the data results show that it is more suitable to replace synthetic cellulose used in the food industry, cosmetics industry, medical materials and paper industry.

Description

Technical Field

[0001] This invention relates to a method for manufacturing bacterial cellulose and its uses, specifically a method for cultivating lignocera acetic acid strains using pineapple peel as a carbon source, and then subjecting the resulting culture solution to emulsion polymerization to obtain bacterial cellulose with high yield, high crystallinity, high heat resistance, and high tensile strength. Prior Technology

[0002] Bacterial cellulose exists in the form of pure cellulose, consisting of straight chains of β-D-glucose linked by β-1,4-glucosidic bonds. These straight chains are parallel to each other and have no branching structure, hence the name β-1,4-glucan. Unlike plant cellulose, which contains lignin and hemicellulose, bacterial cellulose has the following advantages compared to plant cellulose: (1) It has high purity, high crystallinity, high degree of polymerization, and consistent molecular orientation, which is beneficial for the preparation of microfiber products; (2) Its diameter is between 10-100 nm, its elastic modulus is several to tens of times that of plant cellulose, and its tensile strength is high; (3) It has high biocompatibility and produces fewer immune rejection reactions when used in mammals; (4) It can be directly degraded in nature and is an environmentally friendly material; (5) It has excellent water retention and water and air permeability, and can absorb 60-700 times its dry weight in water. Based on these characteristics, bacterial cellulose is currently the best performing cellulose and has high application value and market prospects in the fields of food, biomedicine, special materials, and cosmetics.

[0003] Currently, there are two main types of culture media for bacterial cellulose production: one is chemically synthesized media, formed by compounding various chemical products, such as Hestrin-Schram medium; the other is a compound medium formed by combining various natural products with chemical products. For example, in the Philippines and Hainan, China, a compound medium made primarily from coconut water and pineapple pomace is used to produce natta for the food industry, which has already been industrialized. Additionally, some studies use other natural products as production substrates. For instance, Thompson uses low-solids potato waste liquid as a substrate for fermentation to produce bacterial cellulose; Shimizu uses juice from watermelon rinds and other melons mixed with extracts from vegetables such as onions and carrots as a culture medium, achieving a higher bacterial cellulose yield than Hestrin-Schram medium. Most research reports indicate that, under the same production strain and culture conditions, the bacterial cellulose yield obtained from the compound medium of natural products is higher than that from Hestrin-Schram medium, but the difference is not significant; however, the production cost is lower, and the product can be directly applied to the food industry, making it economically viable.

[0004] From a production process perspective, bacterial cellulose is mainly produced through two methods: dynamic fermentation and static fermentation. Static fermentation produces higher yields of bacterial cellulose, with superior polymerization degree and mechanical properties compared to products from dynamic fermentation. However, static fermentation requires a larger specific surface area and has a longer production time, typically 8 to 16 days. Therefore, improving the yield and efficiency of static fermentation and shortening the fermentation time are research directions in this field.

[0005] Pineapple peels are a common agricultural waste in Taiwan, with known applications including deodorization, removing fishy odors, aiding digestion, making eco-enzymes, organic fertilizer, and in food and beverages. Pineapple peels are rich in bromelain, which helps break down proteins; therefore, adding pineapple peels when stewing meat can aid digestion and remove the meat's fishy smell. In addition, pineapple peels can absorb odors and are often used to deodorize newly renovated rooms or refrigerators. Eco-enzymes made from fermented pineapple peels can be used for cleaning, washing, and deodorizing, making them a natural and environmentally friendly cleaning agent. Pineapple peels can also be used as fertilizer for plants, providing the nutrients needed for plant growth. While current technology allows for the cultivation of bacterial cellulose strains using fruits or peels as carbon sources, there is currently no research on improving the yield, heat resistance, tensile strength, and production time of bacterial cellulose through biopolymerization.

[0006] To address the shortcomings of the prior art in terms of increasing yield, heat resistance, tensile strength, and shortening fermentation time, this invention proposes a new manufacturing method and application to overcome the deficiencies of the existing technology and improve product performance and production efficiency. Summary of the Invention

[0007] In view of this, the present invention provides a method for cultivating lignocellulosic acid bacteria using widely available agricultural waste (pineapple peel) as a carbon source, and combining it with emulsion polymerization to produce bacterial cellulose. Compared with the traditional process using glucose or fructose as a carbon source, this method can significantly improve the yield, crystallinity, heat resistance and tensile strength of bacterial cellulose, and its production time is shorter than the traditional static fermentation time of 16 days, which can effectively improve production efficiency and reduce manufacturing costs.

[0008] The purpose of this invention is to provide a method for manufacturing bacterial cellulose, which includes the following steps: (1) Culture medium preparation: The homogenized pineapple peel liquid is used as the main carbon source. This culture medium contains 20-40% (V / V) pineapple peel juice. (2) Inoculation and fermentation: The lignovinac acid strain was inoculated into the culture medium at an inoculation rate of 5-20% (v / v) and statically cultured at a constant temperature of 25-30℃ to obtain bacterial cellulose; (3) Cleaning and neutralization: The bacterial cellulose was removed, rinsed with pure water, and then placed in sodium hydroxide solution to wash away the residual culture medium. The pH was then adjusted to neutral with acid to obtain purified bacterial cellulose. (4) Emulsion preparation and polymerization reaction: After reacting the acrylic epoxidized soybean oil (AESO) emulsion mixture with the catalyst and oxidant, a reducing agent is added. During the reaction, the AESO emulsion mixture is added in a half-batch feeding manner, and the mixture is continuously stirred until the polymerization reaction is completed to obtain the AESO emulsion; (5) Emulsion polymerization reaction: including mixing the purified bacterial cellulose with the AESO emulsion and reacting under closed conditions, so that the emulsion particles penetrate into the fibrous network structure of the purified bacterial cellulose to form emulsion-polymerized modified bacterial cellulose; (6) Post-processing: The emulsion-polymerized bacterial cellulose is washed to remove surface residues and dried at a suitable temperature until no moisture is present to obtain the emulsion-polymerized bacterial cellulose finished product.

[0009] In one embodiment of the present invention, the method for producing bacterial cellulose includes a sodium hydroxide solution of 0.05-1N and an acid of hydrochloric acid with a concentration of 0.5-5N.

[0010] In one embodiment of the present invention, the method for manufacturing bacterial cellulose, wherein the acrylic epoxidized soybean oil (AESO) emulsion mixture is composed of acrylic epoxidized soybean oil, dibutyl itaconic acid ester, lauryl methacrylate and 1,6-hexanediol diacrylate in a weight ratio between 4-8:3-6:2-4:1-2.

[0011] In one embodiment of the present invention, the method for manufacturing bacterial cellulose includes a half-batch feeding method in which 0.1-0.3% AESO emulsion mixture is added every 5-15 minutes for 60-100 minutes, and after feeding, the mixture is left to stand for 60-120 minutes to allow the monomer reaction to be complete. During the process, the mixture is continuously stirred at 200-500 rpm, and the total polymerization reaction time is 2-5 hours.

[0012] In one embodiment of the present invention, the method for manufacturing bacterial cellulose, wherein the emulsion polymerization reaction takes 2-10 days.

[0013] In one embodiment of the present invention, the method for manufacturing bacterial cellulose, wherein the suitable temperature for drying moisture is 30-80°C.

[0014] In one embodiment of the present invention, the catalyst is ferrous sulfate.

[0015] In one embodiment of the present invention, the oxidant and the reducing agent are hydrogen peroxide and ascorbic acid.

[0016] In one embodiment of the present invention, the method for manufacturing bacterial cellulose is used to prepare a cellulose with improved industrial applicability, wherein the bacterial cellulose is obtained by adding a lignocera cellulose strain to a pineapple peel culture medium as a carbon source and then performing an emulsion polymerization reaction with an AESO emulsion; the improvement in industrial applicability refers to increasing yield, crystallinity, heat resistance and tensile strength, thereby replacing synthetic cellulose used in the food industry, cosmetics industry, medical materials and paper industry.

[0017] In one embodiment of the present invention, the weight ratio of bacterial cellulose to AESO emulsion in the emulsion polymerization reaction is between 0.5-2:2.5-10.

[0018] The bacterial cellulose of this invention is prepared by emulsion polymerization. Compared with the traditional method using glucose or fructose as a carbon source, it has the advantages of high yield, high crystallinity, high heat resistance and high tensile strength. It can also shorten the production time and achieve high efficiency and low cost. Simple Explanation of the Diagram

[0019] Figure 1 is a comparison of bacterial cellulose yields produced by culture in media using glucose, fructose, and pineapple peel as carbon sources.

[0020] Figure 2 shows the trend of sugar consumption in three culture media during the cultivation of acetic acid bacteria.

[0021] Figure 3 shows SEM images of bacterial cellulose (BC) and bio-leather (BC-AESO). (a) BC-G. (b) BC-AESO-G. (c) BC-F. (d) BC-AESO-F. (e) BC-P. (f) BC-AESO-P.

[0022] Figure 4 shows the ATR-FTIR analysis of the biological leather sample.

[0023] Figure 5 shows the XRD diffraction analysis of the biological leather sample. Implementation

[0024] The present invention is illustrated by the following embodiments, but the present invention is not limited to the following embodiments.

[0025] [Terminology Definitions] This specification extensively uses many technical and scientific terms commonly used in the field of biotechnology. In the following description, to provide a clear and consistent understanding of the scope of this specification and the claims, as well as the scope to which these terms are applied, the following definitions are provided. Other terms not specifically defined below have meanings commonly understood by those skilled in the art.

[0026] Unless otherwise specified, all materials used in this invention are commercially available and readily available.

[0027] The words "or," "and," and "and" used in this specification, unless otherwise stated, refer to "or / and." Furthermore, the terms "comprising" and "including" are not restrictive open-ended conjunctions. The foregoing paragraphs are for systematic reference only and should not be construed as limiting the subject of the invention.

[0028] Unless otherwise specified, the "%" used in this instruction manual refers to "weight percentage (wt%)"; numerical ranges (e.g., 10%~11% of A) include upper and lower limits unless otherwise specified (i.e., 10%≦A≦11%); if the lower limit of the numerical range is not defined (e.g., less than 0.2% of B, or B below 0.2%), then the lower limit may be 0 (i.e., 0%≦B≦0.2%); the proportional relationship of "weight percentage" of each component can also be replaced by the proportional relationship of "parts by weight".

[0029] All values ​​disclosed in this specification are subject to a standard technical measurement error (standard deviation) of ±10%. The term "about" is intended to indicate ±10%, ±5%, ±2.5%, or ±1% relative to a given value; that is, "about 20%" represents 20 ±2%, 20 ±1%, 20 ±0.5%, or 20 ±0.25%.

[0030] Example 1: Preparation steps of bacterial cellulose (a1) After adding quantitative growth medium to a sterilized conical flask, add 5-20% (v / v) inoculum of Komagataeibacter xylinus (formerly Acetobacter xylinum and Gluconacetobacter xylinum) from the pre-culture liquid and transfer to a 28℃ constant temperature incubator; the standard medium is Hestrin-Schramm medium, which uses glucose as the carbon source. The yield of bacterial cellulose membranes was compared between acetic acid bacteria culture using fructose or pineapple peel as the carbon source. Bacterial cellulose (BC) obtained using glucose as the carbon source is abbreviated as BC-G, BC obtained using fructose as the carbon source is abbreviated as BC-F, and BC obtained using pineapple peel as the carbon source is abbreviated as BC-P. When using pineapple peel as the carbon source, extract the juice and sterilize it under high temperature and high pressure (121℃, 1.2 atm). (a2) After culturing, remove the BC product and rinse it with pure water. Then place it in a 0.05-1 N sodium hydroxide solution to wash away residual culture medium and sterilize it. The first incubation period is 12-48 hours, and the time is reduced with each subsequent incubation until the BC product is pure. Place the BC product in pure water and adjust the pH back to neutral with 0.5-5 N hydrochloric acid. (a3) At room temperature, first put 4-8 g of Acrylic epoxidized soybean oil, 3-6 g of Dibutyl itaconate, 2-4 g of Lauryl methacrylate, and 1-2 g of 1,6-hexanediol diacrylate into a container as an AESO emulsion mixture. Take 20.5 g of the AESO emulsion mixture as a seed solution, and then add 0.001-0.005 g of FeSO4 catalyst and 0.5-1.5 g of H2O2 oxidant in sequence. After stirring continuously for 20 minutes, add 0.1-1 g of ascorbic acid reducing agent (which needs to be dissolved in a small amount of water first). During the reaction, a semi-batch feeding method is adopted. 3-9 g of AESO emulsion mixture is added every 5-20 minutes within 60-100 minutes for a total of 5-10 times. After the feeding is completed, it is left to stand for 60-120 minutes to allow the monomer reaction to be complete. During the process, the mixture is continuously stirred at 350 rpm. The total polymerization reaction time is 2-5 hours. (a4) Gently press the gel-like BC until it no longer drips, then emulsify it with an AESO emulsion at weight ratios of 0.1:0.5, 0.5:2.5, 1:5, 5:25, and 10:50 (BC:AESO emulsion) in a sealed container. Allow the container to react at room temperature with shaking for 3-6 days. After the reaction is complete, remove the film and wash off any residue with distilled water. Place the cleaned film on baking paper and dry at 40-70°C until completely dry. The finished product obtained by emulsifying BC-G with AESO is called BC-AESO-G; the finished product obtained by emulsifying BC-F with AESO is called BC-AESO-F; and the finished product obtained by emulsifying BC-P with AESO is called BC-AESO-P. Comparing the bacterial cellulose yields produced by culturing in mediums with glucose and fructose as carbon sources under the same inoculum of 5-20% (V / V) of lignovinac strain, as shown in Figure 1, it can be seen that the BC yields in the three mediums were similar in the first three days. However, after the fourth day, BC growth accelerated in the pineapple peel medium until the eighth day, when the yield was 12.9 g / L, which was more than six times that of the glucose (BC-G) yield of 2.1 g / L. For example, the xylinus vinifera can be a commercially available xylinus vinifera, such as the xylinus vinifera purchased from the Hsinchu Biological Resources Conservation and Research Center (BCRC) with the number 80146 (ATCC number 11142).

[0031] Example 2: Measurement of reducing sugar content in culture medium using the dinitrosalicylic acid (DNS) method

[0032] The reducing sugar content in the culture medium for a given number of culture days can be measured using the dinitrosalicylic acid (DNS) method, as shown in Table 1. The sugar consumption can also be calculated from the values ​​in Table 1, as shown in Figure 2. The sugar consumption in the pineapple peel culture medium is higher than that in the other two culture media.

[0033] (Table 1) Analysis of sugar content in three culture media during the cultivation of xylitol acetic acid bacteria Day Glucose concentration (μg / mL) Fructose concentration (μg / mL) Pineapple peel concentration (μg / mL) 0 1000 1000 1000 3 969 974 800 4 931 949 716 5 862 863 689 6 791 858 638 7 636 681 569

[0034] Example 3: Measurement of sample crystallinity by X-ray diffraction (XRD)

[0035] In Table 2, the crystallinity of the unmodified BC samples was around 99%, while the crystallinity of BC-AESO-G and BC-AESO-F after AESO emulsion modification was between 66-67%, and only the BC-AESO-P sample maintained a crystallinity as high as 96.8%. The decrease in crystallinity will lead to a decrease in the strength and thermal stability of BC, but will increase its plasticity. It is speculated that the reason is that during the BC soaking process, the emulsion particles migrate and diffuse into the BC fiber network structure, affecting the molecular chain arrangement of BC.

[0036] (Table 2) Crystallinity of Samples Sample Crystallinity (%) BC-G 99.5 BC-F 99.0 BC-P 99.9 BC-AESO-G 79.1 BC-AESO-F 75.1 BC-AESO-P 96.8

[0037] Example 4: Thermogravimetric analysis to test the heat resistance of bacterial cellulose

[0038] (Table 3) shows the rapid degradation, dehydration, depolymerization of the polymer backbone, and decomposition temperature of glucose in BC; while the maximum pyrolysis temperature Tdmax of BC-AESO is 376.95 ℃, which is higher than that of unmodified BC, confirming that the diffusion of AESO emulsion particles can effectively improve the thermal stability of the material. Therefore, it is more stable and less prone to pyrolysis if further processing is required.

[0039] (Table 3) Thermogravimetric analysis: Weight loss of 5%, 10% and maximum pyrolysis temperature Sample Td5%(℃) Td10%(℃) Tdmax (°C) BC-G 199.92 233.05 318.94 BC-F 158.24 236.79 313.07 BC-P 173.74 245.62 345.05 BC-AESO-G 244.79 282.0 376.95 BC-AESO-F 251.61 314.06 380.57 BC-AESO-P 250.27 314.82 377.92

[0040] Example 5: Mechanical Property Testing of Samples

[0041] Regarding tensile strength (Table 4), this experiment referenced the ASTM D882 standard established by the American Society for Testing and Materials. This standard is mainly used to test the mechanical tensile properties of film and sheet plastic materials. However, the original dog bone-shaped test pieces were changed to rectangular thin sheets with a size of 10 mm x 40 mm. Five test pieces were cut for each group of samples for measurement. It can be seen that the strength of BC cultured with pineapple peel (43.44 MPa) is significantly higher than the other two values. It is speculated that during the manufacturing process of BC, the presence of plant cellulose in the culture medium allows the bacterial cellulose to form a more stable and solid structure. The doping of emulsion particles slightly reduces the tensile strength of BC-AESO, indicating that the emulsion particles after film formation are not conducive to improving the tensile strength of BC.

[0042] (Table 4) Tensile Measurement Results of Bio-leather Samples [Sample] [Thickness (mm)] [Tensile strength (MPa)] BC-G 0.043 ± 0.01 33.57 ± 6.61 BC-F 0.04 ± 0.01 18.91 ± 5.82 BC-P 0.12 ± 0.01 43.44 ± 9.37 BC-AESO-G 0.26 ± 0.01 7.30 ± 1.09 BC-AESO-F 0.393 ± 0.01 15.37 ± 1.40 BC-AESO-P 0.383 ± 0.005 42.30 ± 4.69

[0043] Example 6: SEM analysis of bacterial cellulose structure

[0044] As can be seen from Figure 3, BC has a nano-network fiber structure. (a), (c), and (e) are the structures of BC-G, BC-F, and BC-P, respectively, while (b), (d), and (f) are the structures of BC-AESO-G, BC-AESO-F, and BC-AESO-P, respectively. It can be seen that the emulsion particles use BC fibers as a framework and are successfully embedded in the nano-fiber structure of BC. Unlike the original nano-fiber structure, it has a relatively flat surface, forming a novel material.

[0045] Example 7: Analysis of bacterial cellulose functional groups by ATR-FTIR

[0046] ATR-FTIR was used to detect specific functional groups contained in BC. As shown in Figure 4, the peaks at 3253 cm⁻¹-3263 cm⁻¹ for the three BC sheet samples are -OH stretching vibrations; the peaks at 1057 cm⁻¹ and 1058 cm⁻¹ are COC stretching vibrations of cellulose glycosidic bonds. The sample after emulsion impregnation showed C=O stretching vibrations at 1733 and 1734 cm⁻¹, which can be attributed to ester functional groups in the acrylate emulsion; the small peak at about 830 cm⁻¹ corresponds to the bending vibration of =CH, which is a characteristic of AESO epoxy groups. Therefore, combined with the above, it can be concluded that the AESO emulsion has been combined with BC to form a composite material.

[0047] Example 8: XRD analysis of bacterial cellulose sample crystal form

[0048] The crystallinity of BC was determined using a diffraction analyzer. All samples used BC as the substrate. As shown in Figure 5, the characteristic peaks of typical cellulose type I (100), (110), and (200) crystal planes appear at 14.8°, 16.7°, and 22.8°. In addition to the aforementioned characteristic peaks, no particularly obvious peaks were observed in the three BC-AESO samples. This is presumably because AESO emulsions are amorphous solids after drying, thus exhibiting only a vague broad peak between 19.0° and 20.0° relative to the BC spectrum.

[0049] In summary, the bacterial cellulose produced by the lignocellulosic acid strain cultured using pineapple peel as a carbon source and subjected to emulsion polymerization has the following advantages compared to the control group using other carbon source culture media: (1). High yield: The yield can reach 12.9 g / L on the 8th day of fermentation, which is much higher than the control group with glucose and fructose as carbon sources. (2). High crystallinity: The modified product still maintains 96.8% crystallinity, and its structural stability is better than that of the control group. (3) High heat resistance: The maximum pyrolysis temperature can reach 377.92 ℃, and the processing stability is high. (4) High tensile strength: Both BC-P and BC-AESO-P have high mechanical strength and are suitable for applications with high structural requirements. (5) Resource recycling and low cost: Using agricultural waste pineapple peels as a carbon source not only reduces costs but also conforms to the principles of green environmental sustainability. Therefore, the product made by this invention has the potential to replace synthetic cellulose in the food industry, cosmetics industry, medical materials industry, papermaking industry and other fields, and meets the patent requirements of novelty and inventiveness.

[0050] The foregoing detailed description is a specific illustration of feasible embodiments of the present invention. However, these embodiments are not intended to limit the patent scope of the present invention. All equivalent implementations or modifications that do not depart from the spirit of the present invention should be included within the patent scope of this application. The aforementioned multiple effects fully meet the statutory patent requirements of novelty and inventiveness. Therefore, this application is filed in accordance with the law, and we respectfully request your office to approve this invention patent application to encourage invention.

Claims

1. A method for manufacturing bacterial cellulose, comprising the following steps: (1) Culture medium preparation: pineapple peel homogenate is used as the main carbon source, and the culture medium contains 20-40% (V / V) pineapple peel juice; (2) Inoculation and fermentation: the lignoacetic acid strain is inoculated into the culture medium at an inoculation amount of 5-20% (v / v) and statically cultured under constant temperature conditions of 25-30℃ to obtain bacterial cellulose; (3) Washing and neutralization: the bacterial cellulose is taken out, rinsed with pure water and placed in 0.05-1N sodium hydroxide solution to wash away residual culture medium and then the pH value is adjusted to neutral with 0.5-5N hydrochloric acid to obtain purified bacterial cellulose. ( 4) Emulsion preparation and polymerization reaction: An Epoxidized Soybean Oil Acrylic Acid (AESO) emulsion mixture (composed of Epoxidized Soybean Oil Acrylic Acid, Dibutyl Itaconic Acid, Lauryl Methacrylate and 1,6-Hexanediol Diacrylate in a weight ratio between 4-8:3-6:2-4:1-2) is reacted with a catalyst and oxidant, and then a reducing agent is added. During the reaction, the AESO emulsion mixture is added in a half-batch feeding manner. The half-batch feeding method is 60-100 minutes, and 0.1-0.3% of the AESO emulsion mixture is added every 5-15 minutes for a total of 5-15 times. After the feeding is completed, it is left to stand for 60-120 minutes to allow the monomer reaction to be complete. During the process, the mixture is continuously stirred at 200-500 rpm until the polymerization reaction is completed. The total polymerization reaction time is 2-5 hours to obtain an AESO emulsion; (5) Emulsion polymerization reaction: including mixing the purified bacterial cellulose with the AESO emulsion and reacting under closed conditions, so that the emulsion particles penetrate into the fibrous network structure of the purified bacterial cellulose to form emulsion-polymerized modified bacterial cellulose; (6) Post-treatment: washing the emulsion-polymerized modified bacterial cellulose to remove surface residues and drying it at an appropriate temperature until there is no moisture, so as to obtain the emulsion-polymerized modified bacterial cellulose finished product.

2. The method for manufacturing bacterial cellulose as described in claim 1, wherein the half-batch feeding method is as follows: 0.1-0.3% AESO emulsion mixture is added every 5-15 minutes for 60-100 minutes, and after feeding, it is left to stand for 60-120 minutes to allow the monomer reaction to be complete. During the process, the mixture is continuously stirred at 200-500 rpm, and the total polymerization reaction time is 2-5 hours.

3. The method for manufacturing bacterial cellulose as described in claim 1, wherein the emulsion polymerization reaction takes 2-10 days.

4. The method for producing bacterial cellulose as described in claim 1, wherein the suitable temperature for drying moisture is 30-80°C.

5. The method for producing bacterial cellulose as described in claim 1, wherein the catalyst is ferrous sulfate.

6. The method for producing bacterial cellulose as claimed in claim 1, wherein the oxidant and the reducing agent are hydrogen peroxide and ascorbic acid.

7. A method for manufacturing bacterial cellulose as claimed in any one of claims 1 to 8 is used to prepare a cellulose with improved industrial applicability, wherein the bacterial cellulose is obtained by culturing pineapple peel as a carbon source with the addition of lignocera cellulose strains, and then performing an emulsion polymerization reaction with AESO emulsion; the improvement in industrial applicability refers to increasing yield, crystallinity, heat resistance and tensile strength, thereby replacing synthetic cellulose used in the food industry, cosmetics industry, medical materials industry and paper industry.

8. As described in claim 9, the weight ratio of bacterial cellulose to AESO emulsion in the emulsion polymerization reaction is between 0.5-2:2.5-10.

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