Tissue culture chip kit and method of establishing hydrogel culture structure

Abstract

The present invention relates to a tissue culture wafer kit, comprising: a substrate having a culture tank having an opening and a bottom; and a module having an embedding portion adapted to be embedded in the culture tank, wherein the embedding portion includes a molding layer having a material that is release-resistant to a hydrogel, for establishing a hydrogel culture structure in the culture tank.

Claims

1. A tissue culture wafer kit, comprising: A substrate having a top surface, a bottom surface, and a circumferential surface, the substrate comprising: a culture portion including: a culture tank having an opening and a bottom, the opening being disposed on the top surface; and a module having a proximal end and a distal end, the module comprising: an embedding portion adapted to be embedded in the culture tank, the embedding portion including: a sealing layer located between the proximal end and the distal end for sealing contact with the opening; and a forming layer formed at the proximal end, the material of the forming layer being releaseable from a hydrogel.

2. The tissue culture wafer kit as described in claim 1, wherein, The groove opening is a first length away from the bottom of the groove, and the sealing layer is a second length away from the proximal end. The first length is greater than the second length.

3. The tissue culture wafer kit as described in claim 1, wherein, The forming layer has a raised structure.

4. The tissue culture wafer kit as described in claim 1, wherein, The substrate further includes: a flow portion formed inside the substrate and in fluid communication with the culture portion, the flow portion including: a first channel connected at one end to the culture tank and at the other end to the top surface; and a second channel connected at one end to the culture tank and at the other end to the top surface; wherein the first channel and the second channel are correspondingly arranged.

5. The tissue culture wafer kit as described in claim 4, wherein, The circulation section further includes: a third flow channel, one end of which is connected to the culture tank and the other end of which is connected to the top surface or the peripheral surface; and a fourth flow channel, one end of which is connected to the culture tank and the other end of which is connected to the top surface or the peripheral surface; wherein the third flow channel and the fourth flow channel are correspondingly arranged.

6. The tissue culture wafer kit as described in claim 5, wherein, The culture tank has a flow guiding structure formed on the inner periphery of the culture tank.

7. The tissue culture wafer kit as described in claim 1, wherein: The slot has a limiting groove; and the sealing layer has a limiting member whose configuration is adapted to engage with the limiting groove.

8. A method for forming a hydrogel culture structure, comprising: Step (A): Provide a tissue culture wafer kit as described in any one of claims 1 to 7; Step (B): Take the module and insert the embedding portion into the culture tank to a first depth to define a forming space between the forming layer and the bottom of the tank; Step (C): Provide a flow force to a hydrosol to allow the hydrosol to flow and fill the forming space and to contact the forming layer; Step (D): Apply a curing condition to the hydrosol to form a hydrogel; and Step (E): Remove the module from the culture tank to form a hydrogel culture structure on the surface of the hydrogel.

9. The method as described in request item 8, wherein: The fluid dynamics originate from pressure difference, capillary action, gravity, or any combination thereof; and the curing conditions include photocuring, ionic crosslinking, thermally induced curing, chemical crosslinking, or any combination thereof.

10. The method as described in request item 8, wherein: The hydrogel culture structure is formed corresponding to the surface structure of the molding layer; and / or the hydrogel culture structure includes one or more micropores.

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