Method for collecting plateletsomes and device for collecting plateletsomes

Abstract

This disclosure provides a method for collecting platelet exosomes, comprising the following steps: Whole blood containing a plurality of platelets is introduced into a first reaction chamber, and a plurality of reaction particles are disposed within the first reaction chamber. The internal space of the first reaction chamber is expanded and connected to the external atmosphere, allowing the whole blood to flow through the gaps between at least two adjacent reaction particles to break down the platelets and obtain a fragmented solution. A second reaction chamber is provided, through which the fragmented solution is introduced from the first reaction chamber. The fragmented solution is allowed to stand at a set temperature and time to obtain a product solution containing a plurality of platelet exosomes. This facilitates the collection of platelet exosomes and can improve the activation efficiency of platelet exosomes and the production of cytokines.

Claims

1. A method for collecting platelet exosomes, comprising: infusing whole blood into a first reaction chamber, the whole blood containing a plurality of platelets and a plurality of reaction particles disposed within the first reaction chamber; expanding an internal space of the first reaction chamber and communicating the first reaction chamber with an external atmosphere, such that the whole blood flows through a gap between at least two adjacent reaction particles, the whole blood making solid contact with a plurality of surface surfaces of the reaction particles to break down the platelets and obtain a broken-down solution; and providing a second reaction chamber disposed adjacent to the first reaction chamber. The internal space of the second reaction chamber is expanded such that the broken solution is introduced from the first reaction chamber into the second reaction chamber through a channel structure between the second reaction chamber and the first reaction chamber; and the broken solution is allowed to stand at a settling temperature and a settling time to obtain a product solution containing a plurality of platelet exosomes, wherein the settling temperature is 25°C to 40°C and the settling time is 0.5 hours to 24 hours.

2. The method for collecting platelet exosomes as claimed in claim 1, wherein after the ruptured solution is allowed to stand, the product solution is centrifuged at a centrifuge speed and a centrifuge time to obtain the platelet exosomes, wherein the centrifuge speed is 3000 rpm to 5000 rpm and the centrifuge time is 5 minutes to 15 minutes.

3. The method for collecting platelet exosomes as described in claim 1, wherein each of the reaction particles is made of glass, a metal oxide, or a ceramic.

4. The method for collecting platelet exosomes as described in claim 1, wherein the settling temperature is 30°C to 40°C.

5. The method for collecting platelet exosomes as described in claim 1, wherein the settling time is 3 hours to 24 hours.

6. The method for collecting platelet exosomes as claimed in claim 1, wherein after expanding the internal space of the second reaction chamber, the second reaction chamber is inverted so that the broken-up solution is contained at one end of the second reaction chamber away from the channel structure.

7. The method for collecting platelet exosomes as claimed in claim 1, wherein the surface roughness of each of the reaction particles is from 0.01 μm to 0.5 μm.

8. The method for collecting platelet exosomes as claimed in claim 1, wherein the average particle size of each of the reaction particles is 2 mm to 10 mm.

9. A platelet exosome collection device, comprising: an outer sleeve structure, including: an inlet; a first receiving region, the inlet being disposed at one end of the first receiving region; a first open end, the first open end being disposed at the other end of the first receiving region; and a plurality of reaction particles disposed in the first receiving region; a tubular element movably disposed in the first receiving region and away from the end of the first receiving region, wherein the tubular element overlaps with the first receiving region and the first open end, the tubular element including: a through hole; a second receiving region, the through hole being disposed at one end of the second receiving region and corresponding to the first receiving region; and a second open end, the second open end being disposed at the other end of the second receiving region; and an inner sleeve structure, including: a fixing tube element detachably locked to an inner side of the end of the second receiving region and corresponding to the through hole; The system comprises: a third receiving area, wherein the fixed tube element is disposed at one end of the third receiving area; a third open end, wherein the third open end is disposed at the other end of the third receiving area; and a plug element, movably disposed in the third receiving area and overlapping with the third receiving area and the third open end; wherein... The outer sleeve structure, the tubular element, and the inner sleeve structure are coaxial. A first reaction chamber is formed between the outer sleeve structure and the tubular element, and a second reaction chamber is formed between the third accommodating area and the plug element.

10. The platelet exosome collection device as claimed in claim 9, wherein the surface roughness of each of the reaction particles is from 0.01 μm to 0.5 μm.

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