A cosmetic composition and a method for inhibiting carbamylation

Abstract

This disclosure relates to a cosmetic ingredient and a method for inhibiting aminomethoxylation. The cosmetic ingredient comprises at least one polylysine and at least one extract of a rose species.

Description

Technical Field

[0001] This disclosure relates to the field of cosmetic or dermatological ingredients. In particular, this disclosure relates to a novel cosmetic and / or dermatological ingredient for inhibiting the aminomethionization of proteins. Prior Technology

[0002] Amine methionization involves adding a cyanate group to the amino groups of a protein. Protein methionization is a characteristic of aging. Amine methionization accumulates in the skin and collagen, especially in the reticular dermis, with aging. It is known that collagen methionization inhibits the formation of collagen cross-links, thereby further altering the quality of collagen bundles and the mechanical properties of connective tissue. Inhibiting methionization may be a novel solution for skin aging.

[0003] Since the phenomenon of aminoxidation on the skin is a newly reported one (since 2016), there are few reports on cosmetic ingredients with aminoxidation-inhibiting properties.

[0004] Cosmetic and / or dermatological ingredients are needed to inhibit the aminomethionization of proteins, particularly on the skin. Summary of the Invention

[0005] This disclosure relates to a cosmetic ingredient comprising at least one polylysine and at least one extract of a rose species.

[0006] This disclosure also relates to a method for inhibiting the aminomethionization of proteins, particularly on the skin, comprising applying a cosmetic composition comprising at least one polyisocyanate and at least one extract of a rose species.

[0007] The ingredients in this cosmetic product can inhibit the aminomethanization of proteins in skin cells, thereby achieving anti-aging and whitening effects on the skin.

[0008] Furthermore, compared to polylysine or extracts of rose species alone (such as Chinensis Flower extract), the combination of polylysine and extracts of rose species (such as Chinensis Flower extract) can achieve an unexpected synergistic effect in inhibiting protein aminomethionization. Simple Explanation of the Diagram

[0009] none Implementation

[0010] Unless otherwise indicated, all percentages mentioned herein are weight percentages.

[0011] As used in this article, "carbamylation" refers to the addition of a cyanate group to the amino group of a protein.

[0012] As used in this article, "comprising" means that the list of elements is not necessarily limited to those explicitly stated.

[0013] As used in this article, "cosmetically acceptable" means that the composition or component is suitable for use in contact with human keratinocytes.

[0014] Unless otherwise stated, as used herein, "molecular weight" refers to the weight-average molecular weight.

[0015] Unless otherwise stated: all numerical values ​​are understood to be modified by the word "about", all percentages are based on the total weight of the components, and all ratios are by weight.

[0016] It should be understood that each numerical range explicitly stated herein will include every narrower numerical range falling within such a wider numerical range, as such narrower numerical ranges are all explicitly stated herein. A. Method

[0017] In some embodiments, this disclosure relates to a method for inhibiting the aminomethionization of proteins, particularly on the skin, comprising applying a cosmetic composition comprising at least one polylysine and at least one extract of a rose species.

[0018] Preferably, the cosmetic ingredient is applied topically.

[0019] Preferably, this method can be used to prevent or slow down aminomethodization. B. Cosmetic ingredients

[0020] In some embodiments, the cosmetic composition disclosed herein may be a local component. In one state, such local components may be in solid, liquid, or gel form. In one state, such local components may be water-based or anhydrous. Water-based components may be in the form of emulsion, solution, or dispersion.

[0021] This disclosure relates to a cosmetic ingredient comprising at least one polylysine and at least one extract of a rose species. poly(lysine)

[0022] In some embodiments, polylysine is a polymer based on lysine.

[0023] In some embodiments, polylysine is a polymer based on L-lysine.

[0024] In some embodiments, the polyisocyanate is selected from the group consisting of α-polyisocyanate, ε-polyisocyanate, hyperbranched polyisocyanate, and dendritic polyisocyanate, preferably selected from the group consisting of ε-polyisocyanate and / or dendritic polyisocyanate.

[0025] In some embodiments, the poly(ion) has a molecular weight equal to or greater than 200, or even equal to or greater than 300, or equal to or greater than 400, preferably equal to or greater than 500, or equal to or greater than 800; and / or the poly(ion) has a molecular weight equal to or less than 200,000, or even equal to or less than 150,000, or equal to or less than 100,000, preferably equal to or less than 50,000, or equal to or less than 20,000; and / or the poly(ion) has a molecular weight between 200 and 200,000, or even between 400 and 100,000, or between 600 and 50,000, or between 800 and 20,000, or between 1,000 and 15,000, preferably between 1,000 and 10,000.

[0026] In some embodiments, the content of at least one polylysine is from about 0.00005% by weight to about 0.5% by weight, based on the total weight of the cosmetic composition; more preferably, the content of at least one polylysine is from about 0.0001% by weight to about 0.2% by weight; and even more preferably, the content of at least one polylysine is from about 0.001% by weight to about 0.1% by weight.

[0027] A non-limiting example of polyisocyanate is ε-polyisocyanate (commercially available as a 25% solution from Chisso). Extracts from rose species

[0028] In some embodiments, the extract of the at least one rose species is selected from the group consisting of: plant extracts of rose species, fruit extracts of rose species, flower extracts of rose species, leaf extracts of rose species, stem extracts of rose species, root extracts of rose species, and mixtures thereof; Preferably, the extract of at least one rose species is a flower extract of a rose species.

[0029] In some embodiments, the extract of the at least one rose species is selected from the group consisting of: rose extract, Rosa banksiae extract, Rosa laevigata extract, Rosa grandiflora extract, Rosa perfumeii extract, Rosa fruticosa extract, Rosa canis extract, Rosa laevigata extract, Rosa chinensis extract, Rosa rugosa extract, Rosa rugosa extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract; Preferably, the extract of at least one rose species is a rose extract. More preferably, the extract of at least one rose species is a rose extract.

[0030] In some embodiments, the extract comprises total flavonoids in an amount of 20% to 50% by weight, preferably 25% to 45% by weight, more preferably 30% to 40% by weight, and total polyphenols in an amount of 50% to 80% by weight, preferably 55% to 75% by weight, more preferably 60% to 70% by weight.

[0031] In some embodiments, the extract is obtained by extraction with a solvent selected from the group consisting of polyols (e.g., butylene glycol, propylene glycol, glycerol), ethanol, water, and mixtures of ethanol and water. Preferably, the ratio of ethanol to water in the mixture is in the range of 1:2 to 10:1, more preferably 1:1 to 5:1, and even more preferably 3:2 to 3:1. For example, the extract can be obtained by a method comprising ethanol / water extraction, filtration, column chromatography, concentration, and freeze-drying.

[0032] In some embodiments, the content of an extract of at least one rose species is from about 0.00002% by weight to about 1% by weight, based on the total weight of the cosmetic components; Preferably, the content of the extract of at least one rose species is from about 0.00005% by weight to about 0.1% by weight; More preferably, the content of the extract of at least one rose species is from about 0.00007% by weight to about 0.07% by weight; Even more preferably, the content of extracts from at least one rose species is from about 0.0007% by weight to about 0.07% by weight.

[0033] In some embodiments, the weight ratio between the extract of the rose species and polylysine in the cosmetic composition is about 1:25000 to 2000:1, more preferably 1:4000 to 100:1, more preferably 0.7:1000 to 70:1, and even more preferably 0.7:1000 to 0.7:1.

[0034] A non-limiting example of an extract from the rose genus is rose extract (commercially available under the brand name Rogina from Ingredi Biotech Co., LTD., composition: 70% rose extract / 30% maltodextrin). Other ingredients:

[0035] This local component may further contain the following ingredients: Oil

[0036] Suitable oils include polysiloxanes, esters, vegetable oils, and synthetic oils, including but not limited to those described herein. The oil may be volatile or non-volatile, and preferably in the form of a pourable liquid at room temperature. If present, the oil may range from about 0.5% to about 85% by weight of the total composition, preferably from about 1% to about 75% by weight, and more preferably from about 5% to about 65% by weight.

[0037] Cyclic and linear volatile polysiloxanes are available from various commercial sources, including Dow Chemical Corporation and Momentive (formerly General Electric Silicones). Dow Chemical's linear volatile polysiloxanes are sold under the trademarks Dowsil and Xiameter 244, 245, 344, and 200 fluids. These fluids include hexamethyldisiloxane (viscosity 0.65 cst), octamethyltrisiloxane (1.0 cst), decamethyltetrasiloxane (1.5 cst), dodecylpentasiloxane (2 cst), and mixtures thereof, wherein all viscosity measurements are performed at 25°C.

[0038] Suitable branched volatile polysiloxanes include alkyltrimethyl polysiloxanes, such as methyltrimethyl polysiloxanes, which have the following general formula:

[0039] Methyltrimethylpolysiloxane can be purchased from Shin-Etsu Silicones under the brand name TMF-1.5, and has a viscosity of 1.5 centisole at 25°C.

[0040] Various straight-chain or branched paraffinic hydrocarbons having 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 carbon atoms, preferably 8 to 16 carbon atoms, are also suitable. Suitable hydrocarbons include pentane, hexane, heptane, decane, dodecane, tetradecane, tridecane, and C8-20 isoalkanes. Suitable C12 isoalkanes are manufactured by Permethyl Corporation under the trademark Permethyl 99A. Many commercially available C16 isoalkanes, such as isohexadecane (under the trademark Permethyl R), are also suitable.

[0041] Esters formed by the reaction of carboxylic acids and alcohols are also suitable. Both the alcohol and the carboxylic acid can have aliphatic (C6-30) chains. Examples include hexyl laurate, butyl isostearate, hexadecyl isostearate, cetyl palmitate, isostearyl neopentanoate, stearyl heptanoate, isostearyl isononanoate, stearyl lactate, stearyl octanoate, stearyl stearate, isonononyl isononanoate, etc.

[0042] This ester can also exist in dimer or trimer form. Examples of such esters include diisostearyl malate, neopentyl glycol dioctanoate, dibutyl sebacate, dicetearyl dimer dilinoleate, dicetearyl adipate, diisocetearyl adipate, diisononyl adipate, diisostearyl dilinoleate, diisostearyl fumarate, dioctyl malate, etc.

[0043] Examples of other types of esters include esters derived from arachidonic acid, citric acid, or docosinate, such as triarachidin, tributyl citrate, tristearyl citrate, triC12-13 alkyl citrate, tricaprylin, tricapryl citrate, tridecyl docosinate, tridecyl docosinate; or tridecyl cocoate, tridecyl isononanoate, etc.

[0044] Fatty acids, or synthetic or naturally occurring triglycerides, may also be used in the composition. Both plant and animal sources may be used. Examples of such oils include castor oil, lanolin oil, C10-18 triglycerides, caprylic / capric / triglycerides, sweet almond oil, almond oil, sesame oil, camellia sativa oil, tamanu seed oil, coconut oil, corn oil, cottonseed oil, linseed oil, black ink, olive oil, palm oil, illipe oil, rapeseed oil, soybean oil, grapeseed oil, sunflower seed oil, walnut oil, and similar oils.

[0045] Synthetic or semi-synthetic glycerides are also suitable, such as mono-, di-, and triglycerides of fatty acids from modified natural fats or oils, for example, mono-, di-, or triglycerides of polyols (such as glycerol). In one example, the fatty (C 12-22) carboxylic acid system reacts with one or more repeating glycerol groups. Examples include glyceryl stearate, diisostearate, polyglycerol-3 isostearate, polyglycerol-4 isostearate, polyglycerol-6 ricinoleate, dioleate, diisostearate, tetraisostearate, tricaprylyl glycerate, distearate, linoleate, glyceryl myristate, isostearate, PEG castor oil, PEG oleate, PEG stearate, PEG tallow glycerate, etc.

[0046] Non-volatile polysiloxane oils (both water-soluble and water-insoluble) are also suitable for the composition. The viscosity range of such polysiloxanes at 25°C is preferably greater than 5 cst to 800,000 cst, more preferably 20 cst to 200,000 cst. Suitable water-insoluble polysiloxanes include amine-functionalized polysiloxanes such as amino-terminated dimethyl polysiloxanes. Examples include dimethyl polysiloxane, phenyl dimethyl polysiloxane, diphenyl dimethyl polysiloxane, phenyl trimethyl polysiloxane, or trimethylsiloxyphenyl dimethyl polysiloxane. Other examples include alkyl dimethyl polysiloxanes such as cetyl dimethyl polysiloxane, stearyl dimethyl polysiloxane, docosyl dimethyl polysiloxane, and similar alkyl dimethyl polysiloxanes. surfactants

[0047] The composition may contain one or more surfactants, especially in the case of an emulsion. However, if the composition is also anhydrous, such surfactants may be used, and these surfactants will assist in dispersing polar components, such as pigments. Such surfactants may be polysiloxane-based or organic-based. These surfactants will help form stable emulsions in the form of water-in-oil or oil-in-water. If present, the surfactant may range from about 0.001% to 30% by weight of the total composition, preferably from about 0.005% to 25% by weight, and more preferably from about 0.1% to 20% by weight.

[0048] Polysiloxane surfactants are commonly referred to as dimethyl polysiloxane copolyols or alkyl dimethyl polysiloxane copolyols. In some cases, the number of repeating ethylene oxide or propylene oxide units in the polymer is also specified. For example, a dimethyl polysiloxane copolyol, also known as PEG-15 / PPG-10 dimethyl polysiloxane, refers to a dimethyl polysiloxane with substituted oxygen units containing 15 ethylene glycol units and 10 propylene glycol units on the silicate backbone. One or more of the methyl groups in the above general structure may also be substituted with longer-chain alkyl groups (e.g., ethyl, propyl, butyl, etc.) or ethers such as dimethyl ether, diethyl ether, propyl ether, butyl ether, and similar ethers.

[0049] Examples of polysiloxane surfactants are those sold by Dow Silicones under the following trademarks: Dowsil 3225C Formulation Aid, whose CTFA name is cyclotetrasiloxane (and) cyclopentylsiloxane (and) PEG / PPG-18 dimethylpolysiloxane; or 5225C Formulation Aid, whose CTFA name is cyclopentylsiloxane (and) PEG / PPG-18 / 18 dimethylpolysiloxane; or Dowsil 190 Surfactant, whose CTFA name is PEG / PPG-18 / 18 dimethylpolysiloxane; or Dowsil 193 Fluid, Dowsil 5200, whose CTFA name is lauryl PEG / PPG-18 / 18 methylpolysiloxane; or Abil EM sold by Goldschmidt. 90, whose CTFA name is cetyl PEG / PPG-14 / 14 dimethyl polysiloxane; or Abil EM 97 sold by Goldschmidt, whose CTFA name is bis-cetyl PEG / PPG-14 / 14 dimethyl polysiloxane; or Abil WE 09, whose CTFA name is cetyl PEG / PPG-10 / 1 dimethyl polysiloxane, which is a mixture also containing polyglycerol-4 isostearate and hexyl laurate; or KF-6011 sold by Shin-Etsu Silicones, whose CTFA name is PEG-11 methyl ether dimethyl polysiloxane; KF-6012 sold by Shin-Etsu Silicones, whose CTFA name is PEG / PPG-20 / 22 butyl ether dimethyl polysiloxane; or Shin-Etsu The CTFA name of KF-6013 sold by Silicones is PEG-9 dimethyl polysiloxane; or the CTFA name of KF-6015 sold by Shin-Etsu Silicones is PEG-3 dimethyl polysiloxane; or the CTFA name of KF-6016 sold by Shin-Etsu Silicones is PEG-9 methyl ether dimethyl polysiloxane; or the CTFA name of KF-6017 sold by Shin-Etsu Silicones is PEG-10 dimethyl polysiloxane; or the CTFA name of KF-6038 sold by Shin-Etsu Silicones is lauryl PEG-9 polydimethylsiloxyethyl dimethyl polysiloxane.

[0050] Various types of cross-linked polysiloxane surfactants are also suitable, which are generally referred to as emulsion elastomers containing at least one hydrophilic portion such as a polyoxyalkylene group. Polyoxyalkylene polysiloxane elastomers that can be used in at least one embodiment of this disclosure include Shin-Etsu. Silicones sells its polyoxyalkylene-modified polysiloxane elastomers under the following names: KSG-21, KSG-20, KSG-30, KSG-31, KSG-32, KSG-33; KSG-210, which is a dimethyl polysiloxane / PEG-10 / 15 cross-linked polymer dispersed in dimethyl polysiloxane; KSG-310, which is a PEG-15 lauryl dimethyl polysiloxane cross-linked polymer; KSG-320, which is a PEG-15 lauryl dimethyl polysiloxane cross-linked polymer dispersed in isododecane; KSG-330 (the former dispersed in triisooctanoic acid glycerol) and KSG-340, which are mixtures of PEG-10 lauryl dimethyl polysiloxane cross-linked polymer and PEG-15 lauryl dimethyl polysiloxane cross-linked polymer.

[0051] Polyglycerol-modified polysiloxane elastomers are also suitable, such as those disclosed in PCT / WO 2004 / 024798, the entire text of which is incorporated herein by reference. Such elastomers include Shin-Etsu's KSG series, such as KSG-710, which is a dimethylpolysiloxane / polyglycerol-3 crosslinked polymer dispersed in dimethylpolysiloxane; or lauryl dimethylpolysiloxane / polyglycerol-3 crosslinked polymer dispersed in various solvents such as isododecane, dimethylpolysiloxane, and triisooctyl glycerol, sold under the Shin-Etsu trademarks KSG-810, KSG-820, KSG-830, or KSG-840. Polysiloxanes sold by Dow Silicones under the trademarks 9010 and DC9011 are also suitable.

[0052] The composition may contain one or more nonionic organic surfactants. Suitable nonionic surfactants include alkoxylated alcohols or ethers formed by the reaction of an alcohol with an alkylene oxide, typically ethylene oxide or propylene oxide. Preferably, the alcohol is a fatty alcohol having 6 to 30 carbon atoms. Examples of such components include: stearyl alcohol polyether 2-100, formed by the reaction of stearyl alcohol and ethylene oxide, wherein the number of ethylene oxide units ranges from 2 to 100; dodecyl alcohol polyether 5-30, formed by the reaction of dodecyl alcohol and ethylene oxide, wherein the number of repeating ethylene oxide units ranges from 5 to 30; cetearyl alcohol polyether 2-100, formed by the reaction of a mixture of cetyl alcohol and stearyl alcohol with ethylene oxide, wherein the number of repeating ethylene oxide units in the molecule ranges from 2 to 100; cetyl alcohol polyether 1-45, formed by the reaction of cetyl alcohol and ethylene oxide, wherein the number of repeating ethylene oxide units ranges from 1 to 45, and so on. All unit descriptions include all integers within the range.

[0053] Other alkoxylated alcohols are formed by the reaction of fatty acids and monohydric alcohols, dihydric alcohols, or polyhydric alcohols with epoxides. For example, products of the reaction of C6-30 fatty carboxylic acids and polyhydric alcohols with alkoxylated alcohols, where the polyhydric alcohols are monosaccharides such as glucose, galactose, methyl glucose, and the like. Examples include polymeric alkyl glycols reacting with glyceryl fatty acid esters such as PEG glyceryl oleate and PEG glyceryl stearate; or PEG polyhydroxyalkanoates such as PEG dimerhydroxystearate, wherein the number of repeating glycol units ranges from 3 to 1000.

[0054] Other suitable nonionic surfactants include alkoxylated sorbitan and alkoxylated sorbitan derivatives. For example, alkoxylation (especially ethoxylation) of sorbitan provides polyalkoxylated sorbitan derivatives. Esterification of polyalkoxylated sorbitan provides sorbitan esters, such as polysorbates. For example, polyalkoxylated sorbitan can be esterified with C6-30, preferably C12-22 fatty acids. Examples of such components include polysorbates 20-85, sorbitan oleate, sorbitan sesquioleate, sorbitan palmitate, sorbitan sesquiisostearate, sorbitan stearate, and so on. Moisturizer

[0055] It may also be necessary to include one or more humectants in the composition. If present, such humectants may range from about 0.001% to 25% by weight of the total composition, preferably from about 0.005% to 20% by weight, and more preferably from about 0.1% to 15% by weight. Examples of suitable humectants include diols, sugars, and the like. Suitable diols are in monomer or polymer form and include polyethylene glycol and polypropylene glycol, such as PEG 4-200, which is a polyethylene glycol having 4 to 200 repeating ethylene oxide units; and C1-6 alkyl glycols, such as propylene glycol, butanediol, pentanediol, and the like. Suitable sugars (some of which are also polyols) are also suitable humectants. Examples of such sugars include glucose, fructose, honey, hydrogenated honey, inositol, maltose, mannitol, maltitol, sorbitol, sucrose, xylitol, xylose, etc. Urea is also suitable. Preferably, the humectant used in the composition disclosed herein is C1-6, more preferably C2-4 alkyldiol, and most particularly butanediol. plant extracts

[0056] It may be necessary to include one or more plant extracts in the composition. If so, the recommended range is about 0.0001% to 10% by weight of the total composition, preferably about 0.0005% to 8% by weight, and more preferably about 0.001% to 5% by weight. Suitable plant extracts include extracts from plants (herbs, roots, flowers, fruits, seeds) such as flowers, fruits, vegetables, etc., including yeast fermentation extracts, Padina Pavonica extract, Thermus thermophilis fermentation extract, Camelina sativa seed oil, Boswellia serrata extract, olive extract, Arabian mustard (Arabidopsis thaliana) extract, Acacia dealbata extract, Acer Saccharinum (sugar maple), and Acidophilus ( The following plant extracts are listed: acidopholus, acorus, aesculus, agaricus, agave, agrimonia, algae, aloe, citrus, brassica, cinnamon, orange, apple, blueberry, cranberry, peach, pear, lemon, lime, pea, seaweed, caffeine, green tea, chamomile, willow bark, mulberry, poppy, and those plant extracts described in CTFA Cosmetic Ingredient Handbook, 8th edition, Volume 2, pages 1646-1660.Other specific examples include, but are not limited to, Glycyrrhiza Glabra, Salix Nigra, Macrocycstis Pyrifera, Pyrus Malus, Saxifraga Sarmentosa, Vitis Vinifera, Morus Nigra, Scutellaria Baicalensis, Anthemis Nobilis, Salvia Sclarea, Rosmarinus Officianalis, Citrus Medica Limonum, Panax Ginseng, Siegesbeckia Orientalis, Fructus Mume, Ascophyllum Nodosum, Bifida Ferment Lysate, Glycine Soja Extract, Beta Vulgaris, and Haberlea Rhodopensis, Polygonum cuspidatum, Citrus aurantium dulcis, Vitis vinifera, Selaginella tamariscina, Humulus lupulus, Citrus reticulata peel, Punica granatum, Asparagopsis, Curcuma longa, Menyanthes trifoliata, Helianthus annuus, Hordeum vulgare, Cucumis sativus, Evernia prunatri, Evernia furfuracea, and mixtures thereof. Particulate materials

[0057] The composition disclosed herein may contain particulate material in the form of pigments, inert particles, or mixtures thereof. If present, the suggested range is from about 0.01% to 75% by weight of the total composition, preferably from about 0.5% to 70% by weight, and more preferably from about 0.1% to 65% by weight. In cases where the composition may contain a mixture of pigments and powders, suitable ranges include about 0.01% to 75% pigments and 0.1% to 75% powders, based on the weight of the total composition.

[0058] Particulate matter can be colored powder or colorless powder. Suitable non-coloring powders include bismuth oxychloride, titania mica, fumed silica, spherical silica, polymethyl methacrylate, micronized Teflon, boron nitride, acrylate copolymers, aluminum silicate, aluminum starch octenyl succinate, bentonite, calcium silicate, cellulose, chalk, corn starch, diatomaceous earth, bleaching clay, glycerol starch, lithium bentonite, hydrated silica, kaolin, magnesium aluminum silicate, magnesium trisilylate, maltodextrin, montmorillonite, microcrystalline cellulose, rice starch, silica, talc, mica, titanium dioxide, zinc laurate, zinc myristate, zinc rosinate, alumina, palygorskite, calcium carbonate, calcium silicate, polydextrose, kaolin, nylon, methyl silylated silica, silk powder, sericite, soybean flour, tin oxide, titanium hydroxide, magnesium triphosphate, walnut shell powder, or mixtures thereof. The powders mentioned above can be surface-treated with lecithin, amino acids, mineral oil, polysiloxane, or various other reagents, either alone or in combination. This surface treatment coats the powder surface and makes the particles more lipophilic.

[0059] Suitable pigments can be organic or inorganic. Organic pigments are typically of various aromatic types, including azo, indigo, triphenylmethane, anthraquinone, and xanthine dyes, designated as D&C and FD&C blue, brown, green, orange, red, and yellow. Organic pigments usually consist of insoluble metal salts of certified color additives called lakes. Inorganic pigments include iron oxide, ultramarine, chromium, chromium hydroxide pigments, and mixtures thereof. Red, blue, yellow, brown, and black iron oxides and mixtures thereof are suitable. Vitamins and antioxidants

[0060] The components disclosed herein may contain vitamins and / or coenzymes and antioxidants. If so, it is recommended that the total composition contain 0.001% to 10% by weight, preferably 0.01% to 8% by weight, and more preferably 0.05% to 5% by weight. Suitable vitamins include ascorbic acid and its derivatives such as ascorbyl palmitate, tetrahexyldecyl ascorbate, etc.; B vitamins such as thiamine, riboflavin, pyridoxine, etc.; and coenzymes such as thiamine pyrophosphate, flavin adenine dinucleotide, folic acid, pyridoxal phosphate, tetrahydrofolate, etc. Vitamin E and its derivatives such as vitamin E acetate, nicotinic acid ester, or other esters thereof are also suitable. Additionally, vitamin D and vitamin K are suitable.

[0061] This disclosure will be further described in conjunction with the following examples, which are illustrated for illustrative purposes only. Experimental Examples [Biological Experiment] [] [Reagents used in this experiment:] []

[0062] ε-poly(lysine) (25% solution, commercially available from Chisso) with a number average molecular weight (Mn) of 4090, a weight average molecular weight (Mw) of 4700, and a molecular weight distribution (Mw / Mn) of 1.14; Rose extract (purchased under the trademark Rogina from Ingredi Biotech Co., LTD., composition: 70% rose extract / 30% maltodextrin). This extract was obtained by a method comprising ethanol / water (3:2-3:1) extraction, filtration, column chromatography, concentration, and freeze-drying. The extract contains 30%-40% by weight of total flavonoids and 60%-70% by weight of total polyphenols. TrypLE Express (purchased from Life Technologies); Asian primary fibroblasts (purchased from Biocell); Dulbecco's Modification of Eagle's Medium (DMEM) 4.5 g / L glucose, L-glutamic acid, and sodium pyruvate (purchased from Corning); Fetal bovine serum (purchased from Corning); Anti-homocysteine ​​antibody (pab01012-P, Covalab) Goat anti-rabbit IgG (H+L) cross-adsorption secondary antibody Alexa Fluor™ 488 (A-11008, Invitrogen) Sodium cyanate (S107362, purchased from Aladdin) [Apparatus used in this experiment:] []

[0063] The Operetta CLS high-content imaging system purchased from PerkinElmer. [Stock solution:] []

[0064] Rose extract was dissolved at a concentration of 5% in sterile filtered DMSO to prepare a stock solution. [Experimental Procedure:] []

[0065] Human dermal fibroblasts (HDF) were maintained in a culture established in Duchenne modified Eagle medium (DMEM) containing 10% fetal bovine serum at 37°C in a 5% CO2, 95% humidified incubator, and passaged using TrypLE Express. 1. Inoculate 2 × 10⁴ HDF cells into 24-well plates for 24 hours, then change the culture medium. 2. Prepare 1.5 mM sodium cyanate in the culture medium, with or without diluting polylysine and / or Rogina to the final concentration. 3. HDF was further treated for 48 hours, then fixed with 4% PFA, and immunostained with Alexa Fluor™ 488, a secondary antibody that cross-adsorbs anti-high citrulline antibody and goat anti-rabbit IgG (H+L). 4. Green fluorescence was captured using the Operetta CLS high-content imaging system purchased from PerkinElmer. For each group, at least three image fields were captured, and the fluorescence intensity was calculated as the average gray value using ImageJ. 5. The protection rate is calculated according to the following formula: The "control" in the equation is background data that does not contain sodium cyanide, poly(amino acid), and Rogina. The "induced" in the equation refers to data from the test group containing sodium cyanate but not poly(amino acid) or Rogina. The "sample" in the equation refers to the data from the test group containing sodium cyanate and poly(amino acid) and / or Rogina. In the table below, "Rogina %" refers to samples containing sodium cyanate and Rogina, but not poly(amino acid). The "poly(amino acid) %" in the table below refers to samples containing sodium cyanate and poly(amino acid) but not Rogina. In the table below, "Rogina % + Polyisocyanate %" refers to samples containing sodium cyanate, Rogina, and polyisocyanate. result: sample Rogina: Poly(lysine) ratio Rose extract: poly(amino acid) average SD Protection rate (%) Synergistic effect control group / / 77.4863 2.1950303 / Induced / / 108.7602 6.6652877 / 0.0001% Rogina / / 109.0607 6.2147942 -0.94% / 0.001% polyisocyanate / / 110.31575 3.6820188 -4.95% / 0.1% poly(amino acid) / / 98.5221 3.7954173 32.75% / 0.0001% Rogina + 0.001% polyisocyanate 1:10 0.7:10 101.1424 2.8299578 24.38% yes 0.0001% Rogina + 0.1% polyisocyanate 1:1000 0.7:1000 83.918 3.0066314 79.44% yes sample Rogina: Poly(lysine) ratio Rose extract: poly(amino acid) average SD Protection rate (%) control group / / 77.8969 2.0294044 / Induced / / 98.134444 2.1496552 / 0.001% Rogina / / 88.3121 4.4901865 48.54% / 0.001% polyisocyanate / / 99.8491 2.6391864 -8.47% / 0.001% Rogina +0.001% poly(lysine) 1:1 0.7:1 87.802889 3.6841102 51.05% yes sample Rogina: Poly(lysine) ratio Rose extract: poly(lysine) average SD Protection rate (%) Synergistic effect control group / / 21.1095 1.030038 / / Induced / / 41.3564 2.649087 / / 0.001% Rogina / / 32.3497 3.332912 44.48% / 0.00001% Rogina / / 40.9616 2.544860101 1.95% / 0.0001% polyisocyanate / / 40.906667 1.2785032 2.22% / 0.02% polyisocyanate / / 32.1419 2.296523 45.51% / 0.05% polylysine / / 30.813 1.56012193 52.07% / 0.001% Rogina + 0.0001% polyisocyanate 10:1 7:1 39.0423 0.954341082 11.43% no 0.00001% Rogina + 0.02% polyisocyanate 1:2000 0.7:2000 32.23155556 1.652967945 45.07% no 0.00001% Rogina + 0.05% polyisocyanate 1:5000 0.7:5000 30.7799 1.45428077 52.24% no

[0066] The experimental results were evaluated using the synergistic effect equivalence curve method, as described in the article titled "Synergistic Effects of Plant Derivatives and Conventional Chemotherapeutic Agents: An Update on the Cancer Perspective," Medicina 2019, 55(4), 110, the full text of which is incorporated herein by reference. According to this equivalence curve method, OE refers to the observed effect, and da and db represent the components, respectively. [a] and ingredients [b] dosage (therefore OE (da, db) means the component of da). [a] and db components The observed effect of the combination of [b]; and OE(da) refers to the component of da. [a] refers to the observed effect, and OE(da) means the component of db. When considering the observed effects (b), three mathematical equations can be derived: OE (da, db) = OE(da)+OE (db) (1) OE(da,db)> OE(da)+ OE(db)(2) OE(da,db) <OE(da)+OE(db)(3)

[0067] If the observed effect satisfies equation (1), then the effect of the two components is a simple sum of the single effects, and the two components do not interact and therefore there is no synergistic effect.

[0068] If the observed effect satisfies equation (2), the result represents a true synergistic effect (or multiplicative effect), in which the effect of the two components is greater than the simple sum of the individual effects.

[0069] If the observed effect satisfies equation (3), the result represents the opposite effect of the synergistic effect, because the effect of the two components is less than the simple sum of the individual effects.

[0070] Methods for testing the efficacy of formulations that resist aminomethionization: 1. Equilibrate the full-thickness skin equivalent model (Biocell) at the gas-liquid interface of the culture medium for 24 hours in an incubator with 5% CO2, 95% humidity and 37°C. 2. Replace the assay medium with fresh medium and locally treat the model with the formulation. To induce ammonium cyanate, add sodium cyanate at a concentration of 1M to the assay medium in the bottom wells. 3. The process was repeated 3 times every 24 hours. The tissue was then embedded in OCT and sectioned at low temperature. Aminomethionization was detected by anti-citrulline antibody.

[0071] A known preparation procedure involves combining polylysine with an extract of at least one rose species (particularly rose flower extract) along with commonly used carriers, adjuvants, or excipients to form a formulation. Exemplary formulations and their preparation methods are provided below. All ingredients used in the formulation are well-known in the art and commercially available.

[0072] Example 1 of the formulation: The serum formulation of the present invention is prepared as follows: sequence Element percentage% 1 DI water Up to 100 2 SEPIMAX ZEN 1.0 3 Glycerin 99.7% 2.5 4 1,3-Butanediol 5.0 5 [Rose extract] [ / ] Maltodextrin [0.1] 6 [Polymethyl methacrylate] [0.1] 7 BARGUARD CP 0.5 8 Phenoxyethanol 0.5 1) First add 1 under RT, then homogenize, and slowly add 2; 2) Homogenize the material from step 1) for 15-30 minutes until homogeneous; 3) Add ingredients 3, 4, 5, and 6 to RT and homogenize for 5 minutes until uniform; 4) Add ingredients 7 and 8 and homogenize for 5 minutes until uniform; and 5) Emissions.

[0073] Example 2 of the formulation: The serum formulation of the present invention is prepared as follows: sequence Element percentage% 1 DI water Up to 100 2 1,3-Butanediol 0.5 3 Xanthan Gum (NF-F) 0.2 4 BARSOLVE NS-100 1.0 5 Glycerin 99.7% 2.5 6 1,3-Butanediol 5.0 7 [Rose extract] [ / ] Maltodextrin [0.1] 8 [Polymethyl methacrylate] [0.1] 9 BARGUARD CP 0.5 10 Phenoxyethanol 0.5 11 ARISTOFLEX AVC 0.5 1) Add 1; 2) Mix 2 and 3 thoroughly beforehand, then add them to 1) and mix thoroughly. 3) Mix 4 and 5 thoroughly beforehand, add to 1), and emulsify for 15 minutes; 4) Add ingredients 6, 7, and 8 to RT and homogenize for 5 minutes until uniform; 5) Add ingredients 9 and 10 and homogenize for 5 minutes until uniform; 6) Homogenize, slowly add 11, homogenize for 10 minutes until uniform; and 7) Emissions. Other embodiments

[0074] While the invention has been described with reference to preferred embodiments, it is not intended to limit the scope of the invention to the specific forms described. Rather, it is intended to encompass alternatives, modifications, and equivalents that may be included within the spirit and scope of the invention, as defined by the appended claims. Therefore, other embodiments, including those readily modifiable by those skilled in the art to which this disclosure pertains, are also within the scope of the claims.

[0075] none

Claims

1. A cosmetic composition comprising at least one polylysine and at least one extract of a rose species, wherein the weight ratio of the rose species extract to the polylysine in the cosmetic composition is from 0.7:1000 to 0.7:

1.

2. The cosmetic composition of claim 1, wherein the polylysine is a polymer based on lysine.

3. The cosmetic composition of claim 1 or 2, wherein the polylysine is a polymer based on L-lysine.

4. The cosmetic composition of claim 1 or 2, wherein the polylysine is selected from the group consisting of: α-polylysine, ε-polylysine, hyperbranched polylysine, and dendritic polylysine.

5. The cosmetic composition of claim 1 or 2, wherein the polylysine has a molecular weight between 200 and 200,000.

6. The cosmetic composition of claim 1 or 2, wherein the polylysine has a molecular weight between 1,000 and 10,000.

7. The cosmetic composition of claim 1 or 2, wherein the content of the at least one polylysine is from 0.00005% by weight to 0.5% by weight based on the total weight of the cosmetic composition.

8. The cosmetic composition of claim 1 or 2, wherein the content of the at least one polylysine is from 0.0015% by weight to 0.1% by weight based on the total weight of the cosmetic composition.

9. The cosmetic composition of claim 1 or 2, wherein the extract of at least one rose species is selected from the group consisting of: rose extract, Rosa banksiae extract, Rosa laevigata extract, Rosa grandiflora extract, Rosa perfumeii extract, Rosa fruticosa extract, Rosa canis extract, Rosa laevigata extract, Rosa laevigata extract, Rosa chinensis extract, Rosa rugosa extract, Rosa rugosa extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract, Rosa chinensis extract.

10. The cosmetic composition of claim 1 or 2, wherein the extract of at least one rose species is selected from the group consisting of: plant extracts of rose species, fruit extracts of rose species, flower extracts of rose species, leaf extracts of rose species, stem extracts of rose species, root extracts of rose species, and mixtures thereof.

11. The cosmetic composition of claim 1 or 2, wherein the extract of at least one rose species is a flower extract of a rose species.

12. The cosmetic composition of claim 1 or 2, wherein the extract comprises total flavonoids in an amount of 20% to 50% by weight and total polyphenols in an amount of 50% to 80% by weight.

13. The cosmetic composition of claim 1 or 2, wherein the extract comprises total flavonoids in an amount of 30% to 40% by weight and total polyphenols in an amount of 60% to 70% by weight.

14. The cosmetic composition of claim 1 or 2, wherein the extract is obtained by extraction with a solvent selected from the group consisting of polyols, ethanol, water, and mixtures of ethanol and water.

15. The cosmetic composition of claim 14, wherein the solvent is a mixture of ethanol and water, wherein the ratio of ethanol to water in the mixture is in the range of 1:2 to 10:

1.

16. The cosmetic composition of claim 14, wherein the solvent is a mixture of ethanol and water, wherein the ratio of ethanol to water in the mixture is in the range of 3:2 to 3:

1.

17. The cosmetic composition of claim 1 or 2, wherein the content of the extract of at least one rose species is from 0.00002% by weight to 1% by weight based on the total weight of the cosmetic composition.

18. The cosmetic composition of claim 1 or 2, wherein the content of the extract of at least one rose species is from 0.0007% by weight to 0.07% by weight based on the total weight of the cosmetic composition.

19. A cosmetic product comprising any one of the cosmetic ingredients claimed in claims 1 to 18.

20. Use of a cosmetic composition of any one of claims 1 to 18 for manufacturing a cosmetic for inhibiting the aminomethionization of proteins on the skin.

21. A method for inhibiting aminomethionization of proteins on the skin, comprising applying a cosmetic composition as claimed in any one of claims 1 to 18 or a cosmetic as claimed in claim 19, wherein the cosmetic composition or the cosmetic is applied topically.