Sodium channel protein type 2 subunit alpha (SCN2A) peptide-based molecularly imprinted conductive polymer, peptide-based molecularly imprinted sensing electrode, biosensor, and manufacturing method thereof

Abstract

Sodium channel protein type 2 subunit alpha (SCN2A) has been shown to be closely associated with childhood epilepsy and other neurodevelopmental disorders. This invention provides a molecular imprinting sensing electrode based on an SCN2A epitope peptide, which can be used for electrochemical analysis. This sensing electrode can selectively recognize the SCN2A epitope peptide and, more specifically, the intact SCN2A protein. This invention also provides a biosensor comprising a peptide molecular imprinted conductive polymer, a potentiostat platform, and / or an extended-gate field-effect transistor (EG-FET) platform, capable of detecting intact SCN2A protein at concentrations ranging from 0.001 pg / mL to 1000 pg / mL. Accordingly, this invention not only contributes to more accurate diagnosis and monitoring of SCN2A-related neurological diseases but also provides a technological foundation for the future development of more sensitive and specific biosensors.

Claims

1. A conductive polymer imprinted with a sodium channel protein type 2 subunit alpha (SCN2A) peptide molecule, comprising: a conductive polymer monomer imprinted with a template molecule of SCN2A; the template molecule of SCN2A is selected from any peptide sequence in SEQ ID NO: 1 to SEQ ID NO: 9; the conductive polymer monomer is aniline (AN) and m-aminobenzenesulfonic acid (MSAN).

2. An SCN2A peptide molecular imprinting sensing electrode, comprising the SCN2A peptide molecular imprinting conductive polymer described in claim 1, and an electrode substrate.

3. The SCN2A peptide molecular imprinted sensing electrode as described in claim 2, wherein, The SCN2A peptide molecule imprinted sensing electrode further comprises a two-dimensional material; the two-dimensional material is selected from at least one of the following groups: tetratitanium trinitride (Ti4N3), trititanium carbonitride (Ti3CN), trititanium dicarbide (Ti3C2), dititanium nitride (Ti2N), dititanium carbide (Ti2C), vanadium dicarbide (V2C), V3AlC2, tetravanadium tricarbide (V4C3), niobium dicarbide (Nb2C), tantalum dicarbide (Ta2C), Mo3AlC2, Ta4AlC3, chromium dicarbide (Cr₂C), manganese dicarbide (Mn₂C), and molybdenum dicarbide (Mo₂C). Trimolybdenum dicarbide (Mo₃C₂), hafnium dicarbide (Hf₂C), scandium dicarbide (Sc₂C), zirconium dicarbide (Zr₂C), 1,3-chromium carbide (Cr₁.₃C), 1,3-molybdenum nitride (Mo₁.₃N), 1,3-tungsten carbide (W₁.₃C), triniobium carbide (Nb₄C₃), 1,3-niobium carbide (Nb₁.₃C), 1,3-molybdenum yttrium carbide (Mo₁.₃Y₀.₆C), dimolybdenum nitride (Mo₂N), vanadium dinitride (V₂N), dichromium dinitride (Cr₂N), zirconium dicarbide Nitrides (Zr₂N), tantalum nitride (Ta₂N), hafnium nitride (Hf₂N), tungsten tricarbide zirconium (W₂Zr₂C₃), tungsten tricarbide hafnium (W₂Hf₂C₃), titanium tricarbide niobium (Ti₂Nb₂C₃), molybdenum tricarbide hafnium (Mo₂Hf₂C₃), molybdenum tricarbide vanadium (Mo₂V₂C₃), molybdenum tricarbide zirconium (Mo₂Zr₂C₃), molybdenum tricarbide titanium (Mo₂Ti₂C₃), molybdenum tricarbide tantalum (Mo₂Ta₂C₃), molybdenum tricarbide niobium (Mo₂Nb₂C₃), chromium tricarbide titanium (Cr₂T) i₂C₃), chromium tricarbide tantalum (Cr₂Ta₂C₃), chromium tricarbide niobium (Cr₂Nb₂C₃), chromium tricarbide vanadium (Cr₂V₂C₃), niobium tricarbide tantalum (Nb₂Ta₂C₃), vanadium tricarbide tantalum (V₂Ta₂C₃), vanadium tricarbide niobium (V₂Nb₂C₃), vanadium tricarbide titanium (V₂Ti₂C₃), titanium tricarbide tantalum (Ti₂Ta₂C₃), hafnium tricarbide (Hf₃C₂), zirconium tricarbide (Zr₃C₂), titanium carbonitride vanadium (Ti,V₂CN), niobium carbonitride titanium (Nb,Ti₂CN).

4. A biosensor comprising the SCN2A peptide molecular imprinted sensing electrode as described in claim 2 or 3, and an electrochemical analyzer or an extended gate field-effect transistor.

5. A method for fabricating a biosensor, comprising: step (1) taking an electrode substrate; step (2) preparing a monomer solution from a conductive polymer monomer, wherein, The conductive monomers are aniline and m-aminobenzenesulfonic acid; Step (3) add the template molecule of SCN2A to the monomer solution, and use electrochemical polymerization to imprint the template molecule onto the conductive polymer to obtain a peptide molecule imprinted sensing electrode; Step (4) further connect the peptide molecule imprinted sensing electrode to an electrochemical analyzer or an extended gate field-effect transistor to obtain a biosensor; The template molecule of SCN2A is selected from any peptide sequence in SEQ ID NO: 1 to SEQ ID NO:

9.

6. The method for fabricating the biosensor as described in claim 5, wherein, In step (3), a two-dimensional material is further added to the monomer solution. The two-dimensional material is selected from at least one of the following groups: titanium trinitride (Ti4N3), titanium carbonitride (Ti3CN), titanium dicarbide (Ti3C2), titanium nitride (Ti2N), titanium dicarbide (Ti2C), vanadium dicarbide (V2C), V3AlC2, vanadium tricarbide (V4C3), niobium dicarbide (Nb2C), tantalum dicarbide (Ta2C), Mo3AlC2, Ta4AlC3, chromium dicarbide (Cr₂C), and manganese dicarbide. (Mn₂C), molybdenum dicarbide (Mo₂C), trimolybdenum dicarbide (Mo₃C₂), hafnium dicarbide (Hf₂C), scandium dicarbide (Sc₂C), zirconium dicarbide (Zr₂C), 1,3-chromium carbide (Cr₁.₃C), 1,3-molybdenum nitride (Mo₁.₃N), 1,3-tungsten carbide (W₁.₃C), triniobium carbide (Nb₄C₃), 1,3-niobium carbide (Nb₁.₃C), 1,3-molybdenum yttrium carbide (Mo₁.₃Y₀.₆C), molybdenum dinitride (Mo₂N), vanadium dinitride (V₂N), dichromium Nitrides (Cr₂N), zirconium nitride (Zr₂N), tantalum nitride (Ta₂N), hafnium nitride (Hf₂N), tungsten tricarbide zirconium (W₂Zr₂C₃), tungsten tricarbide hafnium (W₂Hf₂C₃), titanium tricarbide niobium (Ti₂Nb₂C₃), molybdenum tricarbide hafnium (Mo₂Hf₂C₃), molybdenum tricarbide vanadium (Mo₂V₂C₃), molybdenum tricarbide zirconium (Mo₂Zr₂C₃), molybdenum tricarbide titanium (Mo₂Ti₂C₃), molybdenum tricarbide tantalum (Mo₂Ta₂C₃), molybdenum tricarbide niobium (Mo₂Nb₂C₃), chromium tricarbide Titanium (Cr₂Ti₂C₃), Chromium tricarbide tantalum (Cr₂Ta₂C₃), Chromium tricarbide niobium (Cr₂Nb₂C₃), Chromium tricarbide vanadium (Cr₂V₂C₃), Niobium tricarbide tantalum (Nb₂Ta₂C₃), Vanadium tricarbide tantalum (V₂Ta₂C₃), Vanadium tricarbide niobium (V₂Nb₂C₃), Vanadium tricarbide titanium (V₂Ti₂C₃), Titanium tricarbide tantalum (Ti₂Ta₂C₃), Hafnium tricarbide (Hf₃C₂), Zirconium tricarbide (Zr₃C₂), Titanium carbonitride vanadium (Ti,V₂CN), Niobium carbonitride titanium (Nb,Ti₂CN).

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