Method of dispersing magnetic beads

Abstract

A method for dispersing magnetic beads includes the following steps: providing a micropore array wafer, wherein the micropore array wafer includes a micropore array substrate, a cover plate, and microchannels; the micropore array substrate has a micropore array region and includes a plurality of micropores; the cover plate is disposed opposite to the micropore array substrate, and the microchannels are disposed between the cover plate and the micropore array substrate; dispersing a plurality of magnetic beads in a first liquid, wherein the plurality of magnetic beads have a first number; injecting the first liquid containing the plurality of magnetic beads into the microchannels; disposing a magnet below the micropore array wafer to cause the plurality of magnetic beads to settle on the surface of the micropore array substrate or within the plurality of micropores; and injecting a second liquid into the microchannels to remove the first liquid in the microchannels and cause the plurality of magnetic beads settled on the surface to enter the plurality of micropores. The micropore array region has a plurality of micropore units, and one of the plurality of micropores corresponds to one of the plurality of micropore units. The area of ​​the micropore array region divided by the first number of the plurality of magnetic beads yields a first ratio, and the first ratio divided by the area of ​​one of the plurality of micropore units yields a second ratio, wherein the second ratio is greater than 5.

Claims

1. A method for dispersing magnetic beads, comprising: A microvia array wafer is provided, wherein the microvia array wafer comprises: a microvia array substrate having a microvia array region and including a plurality of microvias; a cover plate disposed opposite to the microvia array substrate and including a first opening and a second opening; and a microchannel disposed between the cover plate and the microvia array substrate and respectively connecting the first opening and the second opening; dispersing a plurality of magnetic beads in a first liquid, wherein the plurality of magnetic beads have a first number; injecting the first liquid containing the plurality of magnetic beads into the microchannel; configuring a magnet below the microvia array wafer to cause the plurality of magnetic beads to settle on the surface of the microvia array substrate or within the plurality of microvias; and injecting a second liquid into the microchannel once through the first opening, such that the second liquid passes through the microvia array region once, causing the first liquid in the microchannel to flow towards the microchannel near the second opening end or causing the first liquid in the microchannel to be removed from the second opening, and causing the plurality of magnetic beads settled on the surface to enter the plurality of microvias; The micropore array region has a plurality of micropore units, and one of the plurality of micropores corresponds to one of the plurality of micropore units. The area of ​​the micropore array region divided by the first number of the plurality of magnetic beads yields a first ratio, and the first ratio divided by the area of ​​one of the plurality of micropore units yields a second ratio, and the second ratio is greater than 5. The ratio of the number of magnetic beads dispersed in the plurality of micropores of the micropore array region to the first number of the plurality of magnetic beads is more than 60%.

2. The method of claim 1, wherein the diameter of the plurality of micropores is 5 micrometers to 15 micrometers, the depth of the plurality of micropores is 3 micrometers to 10 micrometers, the side length of the plurality of micropore units is 10 micrometers to 30 micrometers, and the diameter of the plurality of micropores is greater than or equal to 0.5 times the side length of the plurality of micropore units.

3. The method as described in claim 1, wherein the diameter of the magnetic bead is from 1 micrometer to 10 micrometers.

4. The method as described in claim 1, wherein the diameter of the plurality of micropores is greater than or equal to 1.5 times the diameter of the magnetic bead.

5. The method of claim 1, wherein the first quantity of the plurality of magnetic beads is from 1,000 to 300,000.

6. The method of claim 1, wherein the surface magnetic flux density of the magnet is 500 Gauss to 2000 Gauss.

7. The method of claim 1, wherein the first liquid is insoluble in the second liquid.

8. The method of claim 1, wherein the second liquid comprises mineral oil or fluorinated liquid.

9. The method of claim 1, wherein the rate at which the second liquid is injected into the microchannel through the first opening is from 0.5 μL / s to 0.8 μL / s.

10. The method of claim 1, wherein the ratio of the number of micropores containing magnetic beads in the micropore array region to the number of the plurality of micropores is less than 50%.

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