bioreactor

Abstract

A bioreactor comprises: a fixed cylindrical surface extending vertically about an axis; a rotating cylindrical surface extending about an axis and dividing the space between the fixed cylindrical surface and the fixed cylindrical surface in the radial direction opposite to the axis into a pool for forming a culture area, and capable of rotating about the axis; a drive unit that drives the rotating cylindrical surface to rotate about the axis; a bottom plate that divides the pool from below and forms a bottom surface that extends uniformly in the circumferential direction; and an aeration unit that supplies air bubbles formed below the bottom surface from the bottom surface upwards.

Description

[Technical Field]

[0001] This disclosure relates to bioreactors. This application claims priority based on Japanese Patent Application No. 2024-105531 filed on June 28, 2024, the contents of which are incorporated herein by reference. [Previous Technology]

[0002] For example, Patent Document 1 discloses a bioreactor for culturing cells. This bioreactor has a double-cylinder structure comprising a rotating inner cylinder and a fixed outer cylinder. A pool containing culture medium is formed between the inner and outer cylinders. The inner cylinder rotates, thereby forming a Taylor vortex in the culture medium contained in the pool. The Taylor vortex stirs the culture medium, thus promoting cell culture.

[0003] To ensure the stable formation of the aforementioned Taylor vortex, it is preferable that the bottom surface of the pool be a uniformly smooth surface. This is because if there are any protruding parts from the bottom surface of the pool, the flow will be disturbed, leading to the collapse of the Taylor vortex. [Prior Art Documents] [Patent Documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 8-308560 [Summary of the Invention]

[0005] [Problem to be Solved by the Invention] On the other hand, in a pool filled with culture medium, oxygen must be supplied to enable the cells being cultured to respire. Therefore, measures must be in place to ensure the smoothness of the pool bottom and to establish a route for oxygen supply.

[0006] This disclosure provides a bioreactor capable of stably forming a Taylor vortex while simultaneously supplying oxygen to the tank. [Technical means to solve the problem]

[0007] The bioreactor disclosed herein comprises: a fixed cylindrical surface extending vertically about an axis; a rotating cylindrical surface extending about the aforementioned axis and dividing the space between the fixed cylindrical surface and the fixed cylindrical surface in a radial direction opposite to the aforementioned axis into a pool serving as a culture area, and capable of rotating about the aforementioned axis; a drive unit driving the aforementioned rotating cylinder to rotate about the aforementioned axis; a bottom plate portion dividing the aforementioned pool from below and forming a bottom surface that extends uniformly in the circumferential direction; and an air dispersing unit supplying air bubbles formed below the aforementioned bottom surface upwards from the aforementioned bottom surface. [Effects of the Invention]

[0008] According to this disclosure, it is possible to provide a bioreactor that can stably supply oxygen to the tank while stably forming a Taylor vortex.

Implementation Method

[0010] <First Embodiment> (Composition of Bioreactor 1) Hereinafter, the bioreactor 1 according to the first embodiment of the present disclosure will be described with reference to FIGS. 1 to 4. The bioreactor 1 shown in FIG. 1 is an apparatus for cell propagation, viral vector production, or antibody production, and is applicable to the culture of all animal cells. The bioreactor 1 includes: an inner cylinder 10, a drive unit 20, an outer cylinder 30, a top plate 40, a bottom plate 50, an aeration unit 60, a cell supply unit 70, and a liquid supply unit 80.

[0011] (Inner Cylinder 10) The inner cylinder 10 is a cylinder centered on an axis X extending vertically in the past. The diameter of the inner cylinder 10 is constant throughout the entire vertical region. The outer circumferential surface of the inner cylinder 10 is a rotating cylindrical surface 11. The inner cylinder 10 is rotated around the axis X by an electric motor, which serves as a drive unit 20 and is mounted on the axis X.

[0012] (Outer Cylinder 30) The outer cylinder 30 is cylindrical with the axis X as its center, and faces the aforementioned inner cylinder 10 from the outer periphery at intervals in the radial direction. The inner diameter of the outer cylinder 30 is constant throughout the entire region along the axis X. The inner circumferential surface of the outer cylinder 30 serves as a fixed cylindrical surface 31. The space divided between the fixed cylindrical surface 31 and the aforementioned rotating cylindrical surface 11 serves as a pool P supplied with culture medium.

[0013] (Top Plate 40) The opening on one side (i.e., the upper side in the vertical direction) of the outer cylinder 30 along the X-axis is closed by the top plate 40. The top plate 40 is a disk-shaped part centered on the X-axis. An opening for inserting the output shaft of the drive unit 20 is formed at the center of the top plate 40. Furthermore, a hole (air discharge part 110) is formed in a portion of the top plate 40 in the circumferential direction for discharging a portion of the air supplied to the pool P to the outside. The downward-facing surface of the top plate 40 is the top surface 41. A space for air retention is formed between the top surface 41 and the liquid surface.

[0014] (Bottom Plate 50) The opening on the other side (i.e., the lower side in the vertical direction) of the outer cylindrical section 30 is closed by the bottom plate 50. The bottom plate 50 is a disk shaped with the axis X as its center. The upward-facing surface of the bottom plate 50 is the bottom surface 51. That is, the aforementioned pool P is formed by the bottom surface 51, the fixed cylindrical surface 31, the rotating cylindrical surface 11, and the top surface 41.

[0015] The lower end face of the inner cylinder 10 faces the bottom surface 51 with a slight gap between them. The size of this gap is slightly larger than the overall size of the bioreactor 1. The vertical dimension of the gap is, for example, set to 1 / 1000 to 1 / 100 of the vertical length of the outer cylinder 30. The vertical dimension of the gap is, for example, set to 1mm to 10mm.

[0016] (Air Dispersion Section 60) Furthermore, an air dispersion section 60 (air jet head) is provided on the bottom surface 51 for supplying air for cell respiration to the liquid in the pool P. The air dispersion section 60 is housed in a recess 52 formed on the bottom surface 51 of the base plate 50. As shown in FIG3, four recesses 52 are provided on the bottom surface 51 at equal intervals of 90° in the circumferential direction with respect to the axis X. The recesses 52 are rectangular when viewed from the axis X direction, and the long side direction is consistent with the radial direction with respect to the axis X. The side extending in a direction orthogonal to the long side direction extends in the tangent direction of the circle centered on the axis X.

[0017] As shown in Figure 2 or Figure 4, the aeration section 60 includes a bubble forming section 61 and an air supply pipe 62. The bubble forming section 61 is a cylindrical shape integrally formed from a ceramic or three-dimensional fibrous component. That is, the bubble forming section 61 has multiple fine pores on its interior and surface. The air supply pipe 62 is connected to the bubble forming section 61. When air is supplied from the outside through the air supply pipe 62, fine bubbles are generated in the liquid through the fine pores of the bubble forming section 61. The bubble forming section 61 and the air supply pipe 62 are housed within the recess 52 and do not protrude from the bottom surface 51. That is, the aeration section 60 is capable of supplying fine bubbles formed below the bottom surface 51 upwards from the bottom surface 51.

[0018] Preferably, the microbubbles have a diameter of 1 μm to 500 μm. More preferably, the maximum diameter is 150 μm. Most preferably, the maximum diameter is 60 μm. This is because the rising of the bubbles causes turbulent flow, which could lead to the collapse of Taylor vortices. Specifically, larger bubbles have a greater force displacing liquid, thus disrupting the flow. In contrast, microbubbles have a lower rising speed and a smaller force displacing liquid, thus maintaining the formation of Taylor vortices. As a result, the melting rate of oxygen also increases, enabling high-density cultivation.

[0019] (Cell supply section 70, Liquid supply section 80) Furthermore, a tube serving as a cell supply section 70 for supplying cells to the pool P, and a tube serving as a liquid supply section 80 for supplying liquid (culture medium) to the pool P are inserted into the bottom plate section 50. These tubes penetrate the bottom plate section 50 vertically, and their downstream ends open into the pool P. Various storage tanks are connected to the upstream ends. Furthermore, it is preferable to provide pumps for pressurizing liquid and cells at intermediate positions on these tubes.

[0020] Furthermore, a liquid discharge section 90 is provided on the bottom plate 50, which is a pipe used to discharge the liquid (culture medium) in the pool P and to adjust the liquid level appropriately.

[0021] (Effects) The aforementioned bioreactor 1 has a double-cylinder structure consisting of a rotating inner cylinder 10 and a fixed outer cylinder 30. A pool P containing culture medium is formed between the inner cylinder 10 and the outer cylinder 30. The inner cylinder 10 rotates, thereby forming a Taylor vortex in the culture medium contained in pool P. The Taylor vortex stirs the culture medium, promoting cell culture. To ensure stable Taylor vortex formation, it is preferable that the bottom surface 51 of pool P be a uniformly smooth surface. This is because if there is a protruding portion from the bottom surface 51 of pool P, the flow will be disturbed, leading to the collapse of the Taylor vortex.

[0022] On the other hand, oxygen must be supplied to the pool P filled with culture medium to enable the cells being cultured to respire. Therefore, measures must be in place to ensure the smoothness of the bottom surface 51 of the pool P and to establish a path for oxygen supply. To solve this problem, the aforementioned configurations are adopted in this embodiment.

[0023] Based on the aforementioned configuration, the aeration section 60 forms bubbles below the bottom surface 51 of the pool P, therefore it is not necessary to place the aeration section 60 itself in the culture area. Thus, no protrusion is formed on the bottom surface 51. Therefore, bubbles can be supplied to the pool P, and Taylor vortices can be stably formed.

[0024] According to the aforementioned configuration, the bubble forming part 61 is provided within the recess 52. Therefore, the bubble forming part 61 does not protrude upwards beyond the bottom surface 51. Thus, no protrusion is formed on the bottom surface 51. Therefore, bubbles can be supplied to the pool P, and Taylor vortices can be formed stably.

[0025] Based on the aforementioned configuration, the recess 52 extends radially as its long side. This allows for the supply of air bubbles (oxygen) over a wide radial direction to the flow of the culture medium around axis X. Therefore, the cells within pool P can be maintained in a state of high activity.

[0026] The first embodiment of this disclosure has been described above. Furthermore, various changes and modifications can be made to the aforementioned components without departing from the spirit of this disclosure.

[0027] For example, as a variation of the first embodiment, the configuration shown in FIG5 can also be adopted. In the example shown in that figure, four recesses 52 are provided on the bottom surface 51 at equal intervals of 90° in the circumferential direction with respect to the axis X. The recesses 52 are rectangular when viewed from the axis X direction, and the direction of the long side is consistent with the tangent direction of the circle centered on the axis X. The side extending in a direction orthogonal to the direction of the long side extends radially with respect to the axis X.

[0028] Based on this configuration, the projected area of ​​the recess 52 when viewed from the flow direction of the culture medium circumferentially around axis X can be minimized. Therefore, the possibility of flow turbulence caused by the recess 52 can be reduced. Therefore, the growth of Taylor vortices can be promoted, and the culture medium can be stirred efficiently.

[0029] In addition, the number of recesses 52 and air diffusers 60 provided is one example, but it is also possible to provide three or fewer recesses 52 and more than five air diffusers 60. Even in this case, the same effect as described above can be obtained.

[0030] <Second Embodiment> Next, the second embodiment of this disclosure will be described with reference to FIG6. Furthermore, for configurations identical to those in the first embodiment described above, the same reference numerals are used, and detailed descriptions are omitted.

[0031] As shown in FIG. 6, in this embodiment, the recess 52 is formed in a plurality of regions biased towards a portion of the circumferential region centered on the axis X. More specifically, a recess 52 is provided in one of the four regions divided by two straight lines passing through the axis X and orthogonal to each other. The recesses 52 in this region are arranged in three equal angular intervals of 45°. Each recess 52 is the same as in the first embodiment, with its long side direction aligned with the radial direction.

[0032] (Effects) Here, to ensure stable growth of Taylor vortices, it is fundamentally preferable that the bottom surface of the pool is a smooth surface without any recesses. Based on the aforementioned configuration, a minimum number of recesses are limited to a portion of the circumferential region, thereby enabling Taylor vortex growth and recovery from disorder in the remaining circumferential region. Therefore, Taylor vortex growth can be further promoted, and the agitation of the culture medium can be performed more efficiently.

[0033] The second embodiment of this disclosure has been described above. Furthermore, various changes and modifications can be made to the aforementioned components without departing from the spirit of this disclosure.

[0034] For example, in the second embodiment described above, a recess 52 is provided in one of the four circumferentially divided regions. However, recesses 52 can also be provided in two or three adjacent divided regions, depending on indicators such as the flow rate distribution or viscosity coefficient of the culture medium, or in design and specifications. Furthermore, the number of recesses 52 provided in one divided region is not limited to three, and can be appropriately varied. Even in this case, the same effect as described above can be obtained.

[0035] <Third Embodiment> Next, the third embodiment of this disclosure will be described with reference to FIG7. Furthermore, for configurations identical to those in the aforementioned embodiments, the same reference numerals are used, and detailed descriptions are omitted.

[0036] As shown in FIG7, in this embodiment, a guide 53 is provided together with the recess 52. The guide 53 is in the shape of a thin plate and covers a portion of the upstream side of the drive unit 20 in the rotation direction of the opening area of ​​the recess 52 opposite to the bottom surface 51. That is, at least a portion of the upstream side of the total opening area of ​​the recess 52 is reduced due to the guide 53.

[0037] (Effective Effect) According to the aforementioned configuration, a guide 53 is provided in the opening region of the recess 52, on the upstream side in the flow direction of the culture medium. This guide 53 reduces the projected area of ​​the opening region. This maintains the amount of bubble generation and increases the smoothness of the bottom surface 51, thus minimizing the collapse of Taylor vortices caused by the formation of the recess 52. Therefore, it promotes the stirring of the culture medium caused by Taylor vortices, and maintains good cell activity.

[0038] The third embodiment of this disclosure has been described above. Furthermore, various changes and modifications can be made to the aforementioned components without departing from the spirit of this disclosure.

[0039] For example, the guide 53 described in the third embodiment can be combined with the configuration of the first or second embodiment described above. According to this configuration, the effects described in each embodiment can be obtained comprehensively.

[0040] <Fourth Embodiment> Next, the fourth embodiment of this disclosure will be described with reference to FIG8. Furthermore, for configurations identical to those in the aforementioned embodiments, the same reference numerals are used, and detailed descriptions are omitted.

[0041] As shown in FIG. 8, the bioreactor 1 of this embodiment further includes a porous plate 54. The porous plate 54 is disposed along the bottom surface 51 and extends in the circumferential direction along the axis X. Specifically, the porous plate 54 is annular with the axis X as its center. The porous plate 54 closes the recess 52 from above. As the porous plate 54, perforated metal is suitable. In addition, a mesh material formed of multiple fibers is also suitable. In this component, multiple pores are uniformly distributed on the surface.

[0042] (Effective Effect) Here, the Taylor vortex within pool P has the characteristic that even if tiny pores are formed on the bottom surface 51, they are not easily broken if these pores are uniformly distributed. When the diameter of the pores is sufficiently small compared to the size of the vortex, the smoothness of the bottom surface 51 is ensured from a macroscopic perspective. According to the aforementioned configuration, the recess 52 is covered from above by the porous plate 54. Therefore, compared to an open recess 52 configuration, the smoothness and uniformity of the bottom surface 51 in the projection are improved. Therefore, the Taylor vortex is less prone to breakage, thus allowing for more efficient and stable stirring of the culture medium. Therefore, the activity of cells in the culture medium can be maintained at a better level.

[0043] The fourth embodiment of this disclosure has been described above. Furthermore, various changes and modifications can be made to the aforementioned components without departing from the spirit of this disclosure.

[0044] For example, the perforated plate 54 does not necessarily have to extend to all areas in the circumferential direction. As another example, the configuration described in the second embodiment can be adopted in which the perforated plate 54 is provided in a portion in the circumferential direction (see FIG9). According to this configuration, the effects described in each embodiment can be obtained in combination.

[0045] <Fifth Embodiment> Next, the fifth embodiment of this disclosure will be described with reference to FIG10. Furthermore, for configurations identical to those in the aforementioned embodiments, the same reference numerals are used, and detailed descriptions are omitted.

[0046] As shown in FIG10, in the bioreactor 201 of this embodiment, the bottom plate 150 is formed of a porous body. The porous body here refers to a plate-shaped component formed of ceramic or three-dimensional fiber, which has fine pores penetrating its interior and surface.

[0047] Furthermore, an air inlet chamber A, capable of supplying air from the outside, is provided below the base plate portion 150. The air diffuser portion 160 is formed by the base plate portion 150 and the air inlet chamber A.

[0048] (Effective Effect) Here, the Taylor vortex within the pool P has the characteristic that even if fine pores are formed on the bottom surface 51, they are not easily broken if these pores are uniformly distributed. Based on the aforementioned configuration, the entire bottom plate portion 150 is formed as a porous body. Therefore, for example, compared to a configuration where the recesses 52 are distributed, the smoothness and uniformity of the bottom surface 51 on the projection can be improved. Therefore, the Taylor vortex is not easily broken, thus allowing for more efficient and stable stirring of the culture medium. Therefore, the activity of cells in the culture medium can be maintained at a better level.

[0049] The fifth embodiment of this disclosure has been described above. Furthermore, various changes and modifications can be made to the aforementioned configuration without departing from the spirit of this disclosure.

[0050] For example, as shown in FIG11, the air inlet chamber A may be divided into a plurality of partitioned regions S in the circumferential direction by a partition member 160. In other words, the air inlet chamber A is divided into a plurality of partitioned regions S when viewed from the X-axis. According to this configuration, the reduction in strength and rigidity caused by the porous body of the base plate 150 can be compensated for by the partition member 160 that separates each partitioned region. Furthermore, the pressure within the air inlet chamber A can also be homogenized. Therefore, more uniform bubbles can be generated in all partitioned regions.

[0051] <Note> The bioreactor 1 described in each embodiment can be mastered, for example, as follows.

[0052] (1) The first state of the bioreactor 1 includes: a fixed cylindrical surface 31 that extends vertically about an axis X; a rotating cylindrical surface 11 that extends about the aforementioned axis X and divides a pool P, which serves as a culture area, between the fixed cylindrical surface 31 and the fixed cylindrical surface 31 in the radial direction opposite to the aforementioned axis X, and is capable of rotating about the aforementioned axis X; a drive unit 20 that drives the aforementioned rotating cylindrical surface 11 to rotate about the aforementioned axis X; a bottom plate 50 that divides the aforementioned pool P from below and forms a bottom surface 51 that extends uniformly in the circumferential direction; and an air dispersing unit 60 that supplies air bubbles formed below the aforementioned bottom surface 51 from the aforementioned bottom surface 51 upward.

[0053] Based on the aforementioned configuration, it is possible to supply bubbles to the pool P while stably forming Taylor vortices.

[0054] (2) The second state of the bioreactor 1 is the bioreactor 1 as described in (1), wherein the aforementioned bottom plate 50 has a recess 52 that is recessed downward from the aforementioned bottom surface 51, and the aforementioned gas dispersing part 60 includes: a bubble forming part 61 that is disposed in the aforementioned recess 52; and an air supply pipe 62 that supplies air from the outside to the aforementioned bubble forming part 61.

[0055] Based on the aforementioned configuration, it is possible to supply bubbles to the pool P while stably forming Taylor vortices.

[0056] (3) The third state of the bioreactor 1 is the bioreactor 1 as described in (2), wherein the aforementioned recess 52 is extended with the radial direction of the aforementioned axis X as the long side direction.

[0057] Based on the aforementioned configuration, the cells in pool P can be maintained in a state of high activity.

[0058] (4) The fourth state of the bioreactor 1 is the bioreactor 1 as described in (2), wherein the aforementioned recess 52 extends in the tangential direction of the circle centered on the aforementioned axis X.

[0059] Based on the aforementioned configuration, it is possible to promote the growth of Taylor vortices and to efficiently and effectively stir the culture medium.

[0060] (5) The bioreactor 1 of the fifth state is the bioreactor 1 described in any one of (2) to (4), wherein the aforementioned recess 52 is formed in a plurality of regions in the circumferential direction with the aforementioned axis X as the center, biased towards a portion of the region.

[0061] Based on the aforementioned configuration, the growth of Taylor vortex can be further promoted, and the stirring of the culture medium can be carried out more efficiently and effectively.

[0062] (6) The sixth state of the bioreactor 1 is the bioreactor 1 as described in (2), wherein it further comprises: a guide 53, which is a portion of the upstream side of the rotating cylindrical surface in the rotation direction of the opening area of ​​the aforementioned recess 52 relative to the aforementioned bottom surface 51.

[0063] Based on the aforementioned configuration, it is possible to promote the stirring of the culture medium and maintain the cell activity in a good state.

[0064] (7) The bioreactor 1 of the seventh state is the bioreactor 1 described in any one of (2) to (6), wherein it has a porous plate 54 arranged along the aforementioned bottom surface 51 and extending in the circumferential direction of the aforementioned axis X, the aforementioned porous plate 54 closing the aforementioned recess 52 from above.

[0065] Based on the aforementioned configuration, the activity of cells in the culture medium can be maintained in a better state.

[0066] (8) The bioreactor 201 of the eighth state is the same as the bioreactor 201 of (1), wherein the aforementioned bottom plate portion 150 is formed of a porous body, and an air inlet chamber A capable of supplying air from the outside is divided below the aforementioned bottom plate portion 150. The aforementioned air dissipation portion 160 is constituted by the aforementioned bottom plate portion 150 and the aforementioned air inlet chamber A.

[0067] Based on the aforementioned configuration, the activity of cells in the culture medium can be maintained in a better state.

[0068] (9) The bioreactor 201 of the ninth state is the same as the bioreactor 201 of (8), wherein the aforementioned air inlet chamber A is divided into a plurality of partitioned regions S in the circumferential direction.

[0069] Based on the aforementioned configuration, the reduction in rigidity caused by the installation of the air inlet chamber A can be compensated by the partition members provided between the dividing areas S. Furthermore, the pressure within the air inlet chamber A can also be made uniform.

[0070] (10) The bioreactor 1 of the 10th state is the bioreactor 1 described in any one of (1) to (9), wherein the diameter of the aforementioned bubbles is more than 1 μm and less than 500 μm.

[0071] Based on the aforementioned configuration, the possibility of Taylor vortex breaking down in the culture medium can be reduced.

[0072] (11) Bioreactor 1 of the 11th state is the bioreactor 1 of (7), wherein the aforementioned porous plate 54 is only provided on the aforementioned bottom surface 51 in one part of the circumferential direction.

[0073] Based on the aforementioned configuration, the turbulence of the Taylor vortex can be restored in areas where the perforated plate 54 is not present. [Industrial applicability]

[0074] According to this disclosure, it is possible to provide a bioreactor that can stably supply oxygen to the tank while stably forming a Taylor vortex. [Simplified Explanation of the Diagram]

[0009] [Figure 1] is a cross-sectional view showing the configuration of the bioreactor according to the first embodiment of this disclosure. [Figure 2] is an enlarged cross-sectional view of a specific portion of the bioreactor according to the first embodiment of this disclosure. [Figure 3] is a cross-sectional view along line III-III of Figure 1. [Figure 4] is a cross-sectional view along line IV-IV of Figure 2. [Figure 5] is a diagram showing a modified example of the bioreactor according to the first embodiment of this disclosure, and is a cross-sectional view corresponding to line III-III of Figure 1. [Figure 6] is a diagram showing the configuration of the bioreactor according to the second embodiment of this disclosure, and is a cross-sectional view corresponding to line III-III of Figure 1. [Figure 7] is an enlarged cross-sectional view showing a specific portion of the bioreactor according to the third embodiment of this disclosure, and is a cross-sectional view corresponding to line IV-IV of Figure 2. [Figure 8] is a diagram showing the configuration of the bioreactor according to the fourth embodiment of this disclosure, and is a cross-sectional view corresponding to line III-III of Figure 1. [Figure 9] is a diagram showing a modified example of the bioreactor according to the fourth embodiment of the present disclosure, and is a cross-sectional view corresponding to line III-III in Figure 1. [Figure 10] is a cross-sectional view showing the configuration of the bioreactor according to the fifth embodiment of the present disclosure. [Figure 11] is a diagram showing a modified example of the bioreactor according to the fifth embodiment of the present disclosure, and is a cross-sectional view corresponding to line XI-XI in Figure 10.

Claims

1. A bioreactor comprising: a fixed cylindrical surface extending vertically about an axis; a rotating cylindrical surface extending about the aforementioned axis and dividing a pool as a culture area between the fixed cylindrical surface and the fixed cylindrical surface in a radial direction opposite to the aforementioned axis, and capable of rotating about the aforementioned axis; a drive unit driving the aforementioned rotating cylindrical surface to rotate about the aforementioned axis; a bottom plate portion dividing the aforementioned pool from below and forming a bottom surface that extends uniformly in the circumferential direction; and an aeration unit supplying bubbles formed below the aforementioned bottom surface upward from the aforementioned bottom surface, the aforementioned bottom plate portion having a recessed portion recessed downward from the aforementioned bottom surface, the aforementioned aeration unit comprising: a bubble forming portion disposed within the aforementioned recessed portion.

2. The bioreactor as described in claim 1, wherein, The aforementioned air dispersing section further includes: an air supply pipe, which supplies air from the outside to the aforementioned bubble forming section.

3. The bioreactor as described in claim 2, wherein, The aforementioned recess extends the radial direction of the aforementioned axis as the long side direction.

4. The bioreactor as described in claim 2, wherein, The aforementioned concave portion extends in the direction of the tangent to the circle centered on the aforementioned axis.

5. A bioreactor as described in any one of claims 2 to 4, wherein, The aforementioned recesses are formed in a plurality of regions in the circumferential direction centered on the aforementioned axis, with a portion of the region being offset from the center.

6. The bioreactor as described in claim 2, wherein, It further includes: a guide member, which is a portion of the opening area of ​​the aforementioned recess relative to the aforementioned bottom surface, on the upstream side of the aforementioned rotating cylindrical surface in the direction of rotation.

7. A bioreactor as described in any one of claims 2 to 4, wherein, It has a perforated plate arranged along the aforementioned bottom surface and extending in the circumferential direction of the aforementioned axis, wherein the aforementioned perforated plate closes the aforementioned recess from above.

8. The bioreactor as described in claim 1, wherein, The aforementioned base plate is formed of a porous body. An air inlet chamber that can supply air from the outside is divided below the aforementioned base plate. The aforementioned air dissipation part is formed by the aforementioned base plate and the aforementioned air inlet chamber.

9. The bioreactor as described in claim 8, wherein, The aforementioned air intake chamber is divided into multiple zones along the circumference.

10. A bioreactor as described in any one of claims 1 to 4, wherein, The diameter of the aforementioned bubbles is between 1 μm and 500 μm.

11. The bioreactor as described in claim 7, wherein, The aforementioned perforated plate is only provided on one portion of the aforementioned bottom surface in the circumferential direction.

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