Deer antler glue lactic acid bacteria fermentation system

Abstract

A deer antler glue lactic acid bacteria fermentation system includes: a gelatinization device configured to gelatinize deer antler to obtain a gelatinized product; a sterilization device connected to the gelatinization device configured to sterilize the gelatinized product to obtain a sterilized product; a pH adjustment device connected to the sterilization device configured to adjust the pH of the sterilized product to obtain a refined gelatinized solution; a fermentation device connected to the pH adjustment device configured to mix the refined gelatinized solution with lactic acid bacteria broth for fermentation to obtain a fermentation broth; a purification device connected to the fermentation device configured to purify the fermentation broth to obtain a purified product; and a drying device connected to the purification device configured to dry the purified product to obtain a dried purified product.

Description

Deer antler glue lactic acid bacteria fermentation system Technical Field

[0001] This invention relates to a lactic acid bacteria fermentation system, and more particularly to a antler glue lactic acid bacteria fermentation system. Prior Technology

[0002] Deer antler glue, rich in collagen and functional peptides, has wide applications in the pharmaceutical, health product, and cosmetic fields. However, current technologies still suffer from insufficient purification specificity in the preparation and application of peptides.

[0003] Therefore, there is a need for an extraction system for deer antler glue that can extract high-efficiency peptide products with high specificity. Summary of the Invention

[0004] Therefore, the purpose of this invention is to provide a antler gum lactic acid bacteria fermentation system that can prepare polypeptide products with antioxidant effects and high stability.

[0005] This invention provides a deer antler glue lactic acid bacteria fermentation system to solve the problems of prior art. The system includes: a gelatinization device configured to gelatinize deer antler to obtain a gelatinized product; a sterilization device connected to the gelatinization device configured to sterilize the gelatinized product to obtain a sterilized product; a pH adjustment device connected to the sterilization device configured to adjust the pH of the sterilized product to obtain a refined gelatinized solution; a fermentation device connected to the pH adjustment device configured to mix the refined gelatinized solution with lactic acid bacteria broth for fermentation to obtain a fermentation broth; a purification device connected to the fermentation device configured to purify the fermentation broth to obtain a purified product; and a drying device connected to the purification device configured to dry the purified product to obtain a dried purified product.

[0006] The technical means adopted in this invention to solve the problems of prior art is to provide a deer antler glue lactic acid bacteria fermentation system, wherein the purification device includes a centrifuge mechanism connected to the fermentation device and configured to perform centrifugation.

[0007] In one embodiment of this invention, a antler glue lactic acid bacteria fermentation system is provided, wherein the purification device includes an ultrafiltration mechanism connected to the centrifugation mechanism. The ultrafiltration mechanism includes a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and the ultrafiltration mechanism is configured to perform ultrafiltration treatment.

[0008] In another embodiment of this invention, a deer antler glue lactic acid bacteria fermentation system is provided, which further includes a crushing device connected to the gelling device, configured to crush the deer antler before feeding it into the gelling device.

[0009] In one embodiment of this invention, a antler glue lactic acid bacteria fermentation system is provided, wherein the fermentation device is a fermentation device that performs the fermentation treatment at 30 to 37°C for 24 to 72 hours.

[0010] In one embodiment of this invention, a antler glue lactic acid bacteria fermentation system is provided, wherein the lactic acid bacteria liquid contains Lactobacillus rhamnosus strain and Lactobacillus germinatus strain.

[0011] The technology employed in the antler glue lactic acid bacteria fermentation system of this invention enables the preparation of polypeptide products with antioxidant effects and high stability. Simple Explanation of the Diagram

[0012] Figure 1 is a schematic diagram showing a antler glue lactic acid bacteria fermentation system according to a first embodiment of the present invention. Figure 2 is a schematic diagram showing a antler gum lactic acid bacteria fermentation system according to another embodiment of the present invention. Figure 3 is a bar graph showing the free radical scavenging test results of the products obtained from the antler glue lactic acid bacteria fermentation system according to the first embodiment of the present invention. Figure 4 is a line graph showing the stability test results of the product obtained from the antler glue lactic acid bacteria fermentation system according to the first embodiment of the present invention. Implementation

[0013] The following description, based on Figures 1 to 4, illustrates an embodiment of the invention. This description is not intended to limit the implementation of the invention, but rather to provide one possible embodiment.

[0014] As shown in Figure 1, an antler glue lactic acid bacteria fermentation system 100 according to an embodiment of the present invention includes: a gelling device 1, configured to gelatinize antler glue to obtain a gelled product; a sterilization device 2, connected to the gelling device 1, configured to sterilize the gelled product to obtain a sterilized product; a pH adjustment device 3, connected to the sterilization device 2, configured to adjust the pH value of the sterilized product to obtain a refined gelled solution; a fermentation device 4, connected to the pH adjustment device 3, configured to mix the refined gelled solution with lactic acid bacteria liquid for fermentation to obtain a fermentation broth; a purification device 5, connected to the fermentation device 4, configured to purify the fermentation broth to obtain a purified product; and a drying device 6, connected to the purification device 5, configured to dry the purified product to obtain a dried purified product.

[0015] As shown in Figure 1, an embodiment of the present invention, a deer antler glue lactic acid bacteria fermentation system 100, wherein the purification device 5 includes a centrifuge mechanism 51 connected to the fermentation device 4 and configured to perform centrifugation.

[0016] As shown in Figure 1, according to an embodiment of the present invention, a antler glue lactic acid bacteria fermentation system 100 is provided, wherein the purification device includes an ultrafiltration mechanism 52 connected to the centrifugation mechanism 51. The ultrafiltration mechanism 52 includes a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and the ultrafiltration mechanism 52 is configured to perform ultrafiltration treatment.

[0017] As shown in Figure 2, the deer antler glue lactic acid bacteria fermentation system 101 according to another embodiment of the present invention further includes a crushing device 7 connected to the gelling device 1, which is configured to crush the deer antler before feeding it into the gelling device 1.

[0018] According to one embodiment of the present invention, a antler glue lactic acid bacteria fermentation system 100 is provided, wherein the fermentation device 4 is a fermentation device that performs the fermentation treatment at 30 to 37°C for 24 to 72 hours.

[0019] According to one embodiment of the present invention, a lactic acid bacteria fermentation system 100 for antler glue contains Lactobacillus rhamnosus strain and Lactobacillus germinatus strain.

[0020] According to one embodiment of the present invention, a deer antler glue lactic acid bacteria fermentation system 100 is provided. The produced polypeptide product is subjected to a 1,1-diphenyl-2-trinitrophenylhydrazine free radical (2,2-Diphenyl-1-picrylhydrazy, DPPH) scavenging experiment. In this experiment, deer antler glue extracted by conventional techniques and not fermented is used as a control group. The experimental results are shown in Figure 3.

[0021] The results in Figure 3 show that, according to one embodiment of the present invention, the half-maximal inhibitory concentration (IC50) of the polypeptide product produced by the deer antler glue lactic acid bacteria fermentation system 100 is less than 0.5 mg / mL, which is significantly better than that of the unfermented deer antler glue control group, indicating that it has excellent free radical scavenging ability.

[0022] According to one embodiment of the present invention, a deer antler glue lactic acid bacteria fermentation system 100 is provided. The durability of the produced polypeptide product is tested, and the degradation results of its storage at room temperature are analyzed. The results are shown in Figure 4.

[0023] The results in Figure 4 show that when the polypeptide product produced by the antler glue lactic acid bacteria fermentation system 100 provided in one embodiment of the present invention is stored at room temperature for 24 months, the degradation water remains stable at 100%, and no significant degradation occurs.

[0024] The above description and explanation are merely illustrative of preferred embodiments of this invention. Those skilled in the art may make other modifications based on the following defined scope of the patent application and the above description, but such modifications shall still be within the spirit of this invention and the scope of its rights.

[0025] 100, 101: Deer Antler Glue Lactic Acid Bacteria Fermentation System 1: Gelatinizing device 2: Sterilization device 3: pH adjustment device 4: Fermentation device 5: Purification device 51: Centrifuge mechanism 52: Ultrafiltration Mechanism 6: Drying device 7: Crushing device

Claims

1. A deer antler glue lactic acid bacteria fermentation system, comprising: a gelling device configured to gelatinize deer antler to obtain a gelatinized product; a sterilization device connected to the gelling device and configured to sterilize the gelatinized product to obtain a sterilized product; a pH adjustment device connected to the sterilization device and configured to adjust the pH value of the sterilized product to obtain a refined gelatinized solution; a fermentation device connected to the pH adjustment device and configured to mix the refined gelatinized solution with lactic acid bacteria broth for fermentation to obtain a fermentation broth; a purification device connected to the fermentation device and configured to purify the fermentation broth to obtain a purified product; and a drying device connected to the purification device and configured to dry the purified product to obtain a dried purified product.

2. The antler gum lactic acid bacteria fermentation system as claimed in claim 1, wherein the purification device includes a centrifugation mechanism connected to the fermentation device and configured to perform centrifugation.

3. The antler gum lactic acid bacteria fermentation system as claimed in claim 2, wherein the purification device includes an ultrafiltration mechanism connected to the centrifugation mechanism, the ultrafiltration mechanism including a first filter membrane with a pore size range of 0.01 to 0.05 micrometers and a second filter membrane with a molecular weight cutoff range of 0.5 to 2 kDa, and the ultrafiltration mechanism is configured to perform ultrafiltration treatment.

4. The antler glue lactic acid bacteria fermentation system as claimed in claim 1, further comprising a pulverizing device connected to the gelling device, configured to pulverize the antler before feeding it into the gelling device.

5. The antler glue lactic acid bacteria fermentation system as claimed in claim 1, wherein the fermentation apparatus is a fermentation apparatus for performing the fermentation treatment at 30 to 37°C for 24 to 72 hours.

6. The antler gum lactic acid bacteria fermentation system as claimed in claim 1, wherein the lactic acid bacteria liquid contains Lactobacillus rhamnosus strain and Lactobacillus germinatus strain.

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