Nanomaterial-enhanced dual-recognition lateral flow detection device

Abstract

A nanomaterial-enhanced dual-recognition lateral flow detection device is suitable for obtaining visually identifiable information and / or measurable electrical signals after a biological fluid passes through it. The device includes a storage tank for holding the body fluid, a fluid channel through which the body fluid sequentially passes through a pretreatment section, a conjugate section, and a capture reaction section, and two types of traps disposed in the capture reaction section. The pretreatment section is used to retain red blood cells and cell debris in the body fluid, and the conjugate section is used to label specific globulins in the body fluid. When the body fluid is retained in the capture reaction section, at least one of the two traps binds to the labeled specific globulin in the body fluid, thereby generating at least one of visually identifiable information and measurable electrical signals.

Claims

1. A nanomaterial-enhanced dual-recognition lateral flow detection device suitable for the passage of a biological bodily fluid, comprising: a storage tank for holding the bodily fluid; a set of fluid channels communicating with the storage tank and for allowing the bodily fluid to move in a direction away from the storage tank, the fluid channels having a pretreatment section for retaining red blood cells and cell debris in the bodily fluid, a conjugate section for labeling specific globulins in the bodily fluid, and a capture reaction section in the direction away from the storage tank; and two traps disposed in the capture reaction section for binding to the labeled specific globulins in the bodily fluid, and generating at least one of visually identifiable information and measurable electrical signals when at least one of the two traps binds to the labeled specific globulins in the bodily fluid.

2. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 1, wherein, One of the two traps is selected from KM55 antibody, antigen-binding fragment of KM55 antibody, equivalent binding molecule of KM55 antibody, and a combination thereof; the other of the two traps is selected from lectin, lectin derivative, aptamer, receptor protein, equivalent binding molecule with glycosyl recognition function, and a combination thereof.

3. The nanomaterial-enhanced dual-identification lateral flow detection device as claimed in claim 1 or 2 further comprises a nanomaterial disposed in the capture reaction section for attachment of the two captures, the nanomaterial being selected from transition metal carbides, graphene oxide, and combinations thereof.

4. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 1, wherein, The pretreatment section has a first space and a filter material disposed in the first space for retaining red blood cells and cell debris in the body fluid. The filter material is selected from polyester fiber, cellulose, non-woven fabric, glass fiber, and a combination thereof.

5. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 4, wherein, The pretreatment section also has a matrix disposed in the first space and used to alter the body fluid, the matrix being selected from buffer salts, surfactants, blocking proteins, chelating agents, and combinations thereof.

6. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 1, wherein, The conjugate segment has a second space and a marker disposed in the second space, the marker being selected from gold nanoparticles, latex microparticles, carbon nanoparticles, magnetic material nanoparticles, fluorescent nanoparticles, quantum dots, and combinations thereof.

7. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 1, wherein, The fluid channel also has a driving force providing section extending from the capture reaction section away from the pretreatment section, the driving force providing section being used to provide driving force for the body fluid to carry the pretreatment section sequentially through the conjugate section and the capture reaction section.

8. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 7, wherein, The driving force providing section has a third space and a filler disposed in the third space, the filler being selected from superabsorbent fibers, superabsorbent fiber pads, porous absorbent materials, glass fibers, polyester fibers, and combinations thereof.

9. The nanomaterial-enhanced dual-recognition lateral flow detection device as described in claim 1, wherein, The capture reaction section has a main region for the two captures to bind to a specific globulin labeled in the body fluid, and an extension region connected to the main region and used to change the fluid flow in the main region.

10. The nanomaterial-enhanced dual-identification lateral flow detection device as claimed in claim 1 further includes a reading instrument disposed in the fluid channel for quantitative analysis, the reading instrument having a receiving element disposed in the capture reaction section for receiving a measurable electrical signal, and a computing module connected to the receiving element for analyzing the electrical signal.

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