Mushroom variety named ‘RO12756’

Abstract

A new and unique brown variety of the mushroom Agaricus bisporus Imbach was produced by crossing the progeny of brown mushrooms and off-white hybrid mushrooms to bridging cross strains. The resultant hybrid, named ‘RO12756’, exhibits distinct and stable characteristics. Hybrid ‘RO12756’ exhibits a light cap color, thicker cap flesh, rounder cap, faster mushroom development, and high yield, especially when compared to available brown mushroom varieties.

Description

[0001] Latin name: Genus and species: The mushroom cultivars of this disclosure are identified as Agaricus bisporus Imbach.

[0002] Varietal denomination Variety denomination: The variety denomination is ‘RO12756’.BACKGROUND OF THE INVENTION

[0003] The cultivated white button variety of Agaricus bisporus, known as A. bisporus (Lange) Imbach (syn. A. brunnescens Peck), is the predominant mushroom species in cultivation in the world today. After many years in which commercial mushroom sales in the United States were restricted primarily to white-capped A. bisporus mushrooms, there has been a recent trend toward increased sales of brown-capped A. bisporus mushrooms of various types and other so-called exotic strains (species other than A. bisporus). Improved agronomic characteristics can be introduced into the hybrid strains that can influence producer profitability. For instance, strains may have different ancestry, which will be reflected directly by the genotype and indirectly, in some cases, by the phenotypical characteristics. Generally speaking, strains may be differentiated on the basis of traits associated with the hybrid strains, such as, but not limited to, cap shape, surface texture, flesh thickness, color, shelf life, basidial spore number, sporelessness, resistance to infection by, symptoms of, or transmission of bacterial, viral or fungal diseases, amongst many other traits, to give some examples. Many of these traits directly contribute to factors that influence productivity, revenue capture, reduced costs, crop performance, and consumer and market demand.

[0004] In the endeavor to produce new mushroom varieties possessing desirable and improved characteristics, a preferred method is the crossing of homokaryons, ‘homokaryons’ being a multinucleate haploid fungal culture possessing a single line (somatic lineage) that is capable of undergoing plasmogamy with one or more other homokaryons. Each genetically different homokaryon is representative of a parent line in a resultant heterokaryon. The degree of success of each strain and the profitability of producers can be improved by the appropriate selection of the parental lines. Additionally, improved visual appeal and improved shelf-life, amongst many other characteristics, can also contribute to increased sales for mushroom growers in the world today. Thus, there are many characteristics by which a novel strain might be judged as superior in a particular production facility or a sales market, regionally or globally.

[0005] It is also well understood that an industry relying extensively only on a single genetic lineage is at increased risk of unpredictable, catastrophic crop failure on a worldwide scale due to the spread of diseases. To alleviate this type of problem, development and cultivation of new hybrid strains with independent lineages will help to act as a disease break. Further, it is highly desirable to simultaneously provide both genetic diversification and commercially acceptable improved crop characteristics. Thus, the need continues to exist for new brown hybrid strains of Agaricus bisporus with improved and preferred characteristics.BRIEF SUMMARY OF THE INVENTION

[0006] It is an object of the disclosure to provide a brown variety of Agaricus bisporus mushroom with improved commercial characteristics relative to existing brown commercial mushrooms. Strains of the new brown variety, as well as qualities of this new variety, are described herein.

[0007] The following traits have been repeatedly observed and are determined to be the unique characteristics of the novel brown Agaricus bisporus mushroom strains. Specifically, it is an object of the disclosure to provide mushrooms that have one or more (most preferably all) of these following characteristics (relative to brown Agaricus bisporus now being marketed).

[0008] These characteristics in combination distinguish ‘RO12756’ as a new and distinct brown variety:

[0009] 1. Faster or as fast timing to reach pinset, first break, and second break as marketed brown varieties.

[0010] 2. High yield, especially during the second and third break.

[0011] 3. Cap apex flesh thicker than marketed brown varieties.

[0012] 4. Cap edge flesh thicker than marketed brown varieties.

[0013] 5. Soft cap firmness.

[0014] 6. Light brown cap color.

[0015] 8. Stipes more wide or as wide as marketed brown varieties.

[0016] 9. Higher or as high tray coverage as marketed brown varieties.

[0017] 10. Incompatibility with other available brown strains.

[0018] These and other objects of the disclosure have been accomplished by providing a hybrid Agaricus bisporus mushroom strain obtained by crossing a mushroom of wild strain AA-0096 or a progeny thereof with a second Agaricus bisporus strain comprising a commercial off-white hybrid strain (Amycel® XXX and Amycel® Delta), wherein the mushroom of the disclosure (1) has (a) at least one genetic characteristic of wild strain AA-0096 not present in the second Agaricus bisporus strain, (b) at least one genetic characteristic of the second Agaricus bisporus strain not present in wild strain AA-0096, and (c) at least one genetic characteristic of the bridging cross strain and (2) has at least one physical characteristic selected from the group consisting of pinset speed, cap color, cap apex flesh thickness, cap edge flesh thickness, cap texture, tray coverage, incompatibility with other strains, speed of harvestable yield in each break, amount of yield in each break, and overall yield that is statistically different from the corresponding physical characteristic of comparison strains ‘BR06’ (U.S. Pat. No. 7,608,760 B1) or ‘B14528’ (U.S. Pat. No. 9,017,988 B1).

[0019] Another feature of the present disclosure includes a new brown hybrid mushroom having improved agronomic traits leading favorably to increased consumer and market demand, productivity, crop performance, and revenue. Of particular interest and novel advantage are the following traits exhibited by ‘RO12756’:

[0020] 1. Higher overall yield, driven by increased yield at later breaks, which enable growers to achieve higher yield per unit area compared to other commercial brown mushrooms.

[0021] 2. Faster developmental timing, which enables growers to increase their efficiency by allowing them to pick off mushrooms before the end of the growing cycle. Faster strains might also allow for more crops per year. Traits herein related to developmental timing include Days to Pinset, Days to 1st Break, Days to 2nd Break, and Percent Harvested Speed.

[0022] 3. Thick-fleshed morphology, which reflects higher quality than other commercial brown mushrooms. This morphology remains more consistent at later breaks for ‘RO12756’ when compared to other commercial varieties, and this increases visual appeal of sliced product. Pre-sliced button mushrooms have been growing in popularity over the last 10+ years; a 10-year report on mushroom sales trends demonstrated that sliced product has eclipsed whole product fresh mushroom sales, encompassing over 50% of fresh brown mushrooms sold in 2023 in the United States (Roerink, A. (2023 October). Ten-year Trends in Fresh Mushroom Sales. Mushroom Council. Available on the worldwide web at [dot]mushroomcouncil[dot]org / wp-content / uploads / 2023 / 10 / Ten-Year-Fresh-Mushroom-Trends[dot]pdf).BRIEF DESCRIPTION OF THE DRAWINGS

[0023] FIG. 1 shows a representative comparison of Agaricus bisporus variety ‘BR06’ (left) and variety ‘RO12756’ (right) tray coverage at first break. ‘BR06’ displays lower tray coverage, and ‘RO12756’ displays higher tray coverage.

[0024] FIG. 2 shows a longitudinal slice of a mushroom of variety ‘RO14176’ with markings added to illustrate the calculation of Cap Edge Flesh Thickness, where Cap Edge Flesh Thickness is calculated by ((LGL−CSL)+(CSR−RGR)) / CSD. LGL=left-most x-coordinate of the left gill, CSL=left-most x-coordinate of the cross-section, CSR=right-most x-coordinate of the cross-section, RGR=right-most x-coordinate of the right gill, and CSD=diameter of the cross-section.

[0025] FIG. 3 shows a longitudinal slice of a mushroom of variety ‘RO14176’ with markings added to illustrate the calculation of Cap Apex Flesh Thickness, where Cap Apex Flesh Thickness is calculated by (CST−((LGT+RGT) / 2)) / CSH. CST=the top-most y-coordinate of the cross-section, LGT=the top-most y-coordinate of the left gill, RGT=the top-most y-coordinate of the right gill, and CSH=the height of the cross-section.

[0026] FIG. 4 shows a representative comparison of cap colors for variety ‘BR06’ (left), variety ‘B14528’ (center), and variety ‘RO12756’ (right) cap colors based on Cap L* Value, Cap a* Value, and Cap b* Value at first break. Mushrooms pictured were grown in the same room, under the same environmental conditions, and were harvested from the same trial in May 2025. All photos have the same scale. Scale bar=1 cm.

[0027] FIG. 5 shows representative comparisons of cap height, relative cap height, and relative cap flesh thickness for variety ‘B14528’ (left), variety ‘BR06’ (center), and variety ‘RO12756’ (right) at first break. Mushrooms pictured were grown in the same room, under the same environmental conditions, and were harvested from the same trial in May 2025. All photos have the same scale. Scale bar=1 cm.DETAILED BOTANICAL DESCRIPTION

[0028] In mushroom breeding, mycelial (=vegetative) cultures of two compatible progenitors (typically these are haploid homokaryotic strains called homokaryons) must come into physical contact so that one or more fusion zones can occur between the progenitors. Within those fusion zones nuclei from the two progenitors become associated. In Agaricus bisporus, a novel, hybrid mycelium ultimately containing two compatible haploid nuclear types (one from each of the two progenitors) plus one mitochondrial type (from either one of the progenitors) emerges. This novel hybrid mycelium can be isolated and propagated to provide the new hybrid culture, which can be further subdivided and propagated for commercial or other purposes.

[0029] It should be recognized that the mushrooms of the present disclosure are hybrids (equivalent to crosses), as they are formed by the hybridization of at least two independently derived strains, since they were formed by hybridizing the wild strain AA-0096 with a second, commercialized strain of A. bisporus (with the second strain, in some embodiments, being itself a cross between at least two other identified strains of A. bisporus, i.e., Amycel® XXX and Amycel® Delta). The term ‘hybrid mushroom’ may be used herein to represent the product of a cross between a wild A. bisporus strain and a commercial strain, often including a cross between a brown mushroom strain and an off-white hybrid variety (Amycel® XXX and Amycel® Delta). Differences in terminology (and in the specific techniques used alongside the terminology) are merely indicative of the variety of terminology and techniques used in the mushroom production field.

[0030] After parental homokaryotic spores germinated into mycelia, single spore isolates (SSIs) were selected, preferentially those exhibiting slow mycelial growth. The slowly growing mycelia of SSIs usually contain one nuclear type, are thus homokaryotic, and are suitable for crossing, and thus for breeding. Slow growers are denoted as “sg”. Some homokaryons were obtained through protoplasting; this technique involves treating the vegetative mycelia of the heterokaryons to damage the fungal cell wall, then selecting colonies of protoclones that are each derived from a single, regenerated protoplast. All crosses were initially cultivated on a small scale, and spores were collected for the parents of the crosses to produce the hybrids described herein.

[0031] One useful feature in the breeding programs for these novel mushroom varieties is the use of a bridging cross strain, which is a hybrid variety produced from crossing a commercial brown variety with a commercial white variety. The particular bridging cross strain of note in these breeding programs is known as the 4x29 strain. Strain 4x29 has been previously deposited under the provisions of the Budapest Treaty with the American Type Culture Collection, Rockville, Md., USA, ATCC accession No. PTA-6877 (deposited on Jul. 20, 2005). Strain 4x29 is the product of crossing a commercial brown strain with a commercial white strain. The ‘29’ of this strain is a unique homokaryon collected from an off-white hybrid strain.

[0032] The varieties, mushrooms, strains, and cultures disclosed herein are understood to comprise parts of mushrooms, such as the fruiting body, cap, stipe, basidium, spore, mycelium, mixed or unmixed spawn, inoculated substrate, protoplast, gill, cell, or product produced from the mushroom. In some embodiments, the mushroom part from the hybrid mushroom produced by any method disclosed herein is a spore. In some embodiments, the mushroom part produced from a mushroom strain disclosed herein is a spore.Trait Determinations

[0033] In the descriptions and tables that follow, numerous traits are calculated / measured as follows in Table 1. Visual depictions of the measurements used for calculating Cap Apex Flesh Thickness are shown in FIG. 3. Visual depictions of the measurements used for calculating Cap Edge Flesh Thickness are shown in FIG. 2.

[0034] Mushrooms were harvested at Stage 3 as described by Hammond & Nichols (Hammond, J. B. W., & Nichols, R. (1976). Carbohydrate metabolism in Agaricus bisporus (Lange) Sing.: changes in soluble carbohydrates during growth of mycelium and sporophore. Microbiology, 93(2), 309-320), when cap diameters were approx. 3-5 cm with the veil still intact. To reduce confounding effects introduced by different trials, developmental timing and yield traits were compared only from trials where all strains being compared were present together.

[0035] Images of mushroom caps and longitudinal cross-sections were captured using a Canon Rebel SL1 camera (Canon, Inc., Toyko, Japan) in a custom-built photo box with standardized lighting conditions. The camera was calibrated for color consistency using a ColorChecker gray balance card (Calibrite LLC, Wilmington, DE). L*, a* and b* values were captured across the surface of each mushroom cap and cross-section using ImageJ (Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671-675). Images captured in 2024 and 2025 were used for analysis. Extreme outliers were excluded from the data, defined as those falling 3 times the interquartile range (IQR) above the 75th percentile for that trait or more than 3 times the IQR below the 25th percentile for that trait.

[0036] All statistical analyses were carried out in the R programming language (R Core Team, (2013). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available on the worldwide web at [dot]R-project[dot]org / ). Levene's test was performed using the ‘car’ package (Fox, J., & Weisberg, S. (2018). An R companion to applied regression. SAGE Publications), Shapiro-Wilk tests and Tukey HSD were performed using the ‘stats’ package (R Core Team (2013)), and standard ANOVAs, Kruskal-Wallis tests and Dunn's tests were performed using the ‘rstatix’ package (Kassambara, A (2023). rstatix: Pipe-Friendly Framework for Basic Statistical Tests. R package version 0.7.2, rpkgs[dot]datanovia[dot]com / rstatix / ).

[0037] Table 1. Trait descriptions corresponding to the information presented in the “Objective description of the variety ‘RO12756’’’ and the “Comparisons to Commercial Mushroom Varieties” sections.

[0038] TABLE 1Measurement TraitToolScaleTimingCap visually0 to 5 (from 1st Color assessedwhite to darkbreak **,Dark-brown), tray-2ndnesslevel break **measurementCap image −128 to +127 1st Color analysis(from pure break **,a* green to pure2ndValuered), individual break **(Green-mushroom-levelRed)measurementCap image −128 to +127 1st Color analysis(from pure bluebreak **,b* to pure2nd Valueyellow), break **(Blue-individualYellow)mushroom-levelmeasurementCap image 0 to 100 (from 1st Color analysispure black break **,L* to pure white),2nd Valueindividual break **(Dark-mushroom-level Light)measurementCap image millimeters 1st Dia-analysis(mm),break **,meterindividual 2nd mushroom-break **level measurementCap manual1 to 5 (from 1st Firm-squeeze verybreak **,nesstestsoft to 2nd very firm),break **tray-level measurementCap calculateddistance from 1st Apex ratio andtop of gills tobreak **,Fleshimage top of cap of2nd Thick-analysismushroom, break **nessrelativeto cap height,individual mushroom-level measurementCap calculatedsum of distances 1st Edge ratio andfrom outer break **,Fleshimage edge of each2nd Thick-analysisgill to outer break **nessedges of capof mushroom,relative to cap diameter, individualmushroom-level measurementCap hand-millimeters (mm),1st Flesh measure-distance from break **,Thick-menttop of2nd nessgill-stipe break **connection (at center of stipe) totop of cap, individual mushroom-levelmeasurementCap image millimeters (mm), 1st Heightanalysisdistance break **,from base of2nd cap to top ofbreak **cap, individual mushroom-level measurementCap image Unitless 1st Round-analysisratio (0-1),break **,nesscalculated 2nd (Cal-as 4 * CSA / break **culated)(π * CSD2), where CSA = area of the cross-section, and CSD = diameter of the cross-section;assessed with imageanalysis, individualmushroom-level measurementCap visually1 to 5 (from 1st Round-assessedvery flat break **,nessto spherical), 2nd (Visual)tray-level break **measurementCompat-cal-pounds per square 1stibilityculatedfoot (lb / ft2) andbreak **ratiokilograms per square meter (kg / m2), weight of 1st break mushrooms yieldedrelative toarea of tray, tray-levelmeasurementCompost visually0 to 5 (from no before Coloniz-assessedcolonization1st breakationto robust(10 days colonization), aftertray-level spawning)measurementDays tovisuallynumber of days 1st1st assessedelapsed after break **Breakcasing at which at least half of tray is of harvestablematurity for 1st break,tray-levelmeasurementDays tovisuallynumber of days 2nd 2nd assessedelapsedbreak **Breakafter casing atwhich at least half of tray isof harvestablematurityfor 2nd break, tray-levelmeasurementDays to visuallynumber of days before Pinsetassessedelapsed 1st breakafter casing atwhich pins firstreach 5 mm,tray-levelmeasurementPercent cal-% of yield 17 days Har-culatedharvested by aftervestedratioa designated datecasing Speedwithin break,(1st break), yield at date 24 dayswithin break / after casing total yield for(2nd break), break, 32 daystrial-level after casing measurement(3rd break)Relative cal-Cap Flesh 1st Cap culatedThickness break **, FleshratioValue / Cap 2ndThick-Diameterbreak **nessValue, individual mushroom-levelmeasurementRelative cal-Cap Height 1st Cap culatedValue / Capbreak **, HeightratioDiameter Value,2ndindividual break **mushroom-levelmeasurementRelative cal-Stipe Diameter 1st Stipe culatedValue / break **, Dia-ratioCap Diameter2ndmeterValue, break**individual mushroom-levelmeasurementStipe hand-millimeters 1st Dia-measure-(mm), stipebreak ** , metermentdiameter 2ndat base ofbreak **cap, individual mushroom-levelmeasurementTray visually0 to 5 (from1st Cover-assessedno coverage to break **, agefull coverage),2ndtray-level break **measurementYield weighingkg / m2, Assessed(Each tray-level cumulativelyBreak)measurement; throughout or lb / ft2, 1st,tray-level2nd, and measurement3rd breaksYield weighingkg / m2, Assessed(Total)tray-levelcumulativelymeasurement;across 1st, or2nd, and lb / ft2, 3rd breakstray-levelmeasurement** = at date when at least half of tray is of harvestable maturity in breakObjective Description of the Variety ‘RO12756’

[0039] The following detailed description sets forth the breeding procedures and the characteristics of new cultivar designated ‘RO12756’. The strain is maintained and asexually propagated as vegetative mycelium. Spawn (inoculum for the substrate compost) is prepared from the vegetative mycelium. Specifically, the strain has been asexually propagated and maintained by vegetative mycelium in Royal Oaks, California, USA. The new variety was tested on a scale of hundreds of individual mushrooms. The variety has shown uniformity and stability for the traits, within the limits of expected environmental influence for the traits.

[0040] All homokaryons utilized are strains of Agaricus bisporus.

[0041] In a first cross, a bridging cross strain 4x29 slow grower (sg) was crossed with a commercial off-white hybrid strain protoplast.

[0042] ‘RO12756’ was produced through the cross of a homokaryon SSI of the first cross to a homokaryon SSI of strain ‘RO10425’ (U.S. application Ser. No. 19 / 305,679).

[0043] The nearest color on the Colour Chart of The Royal Horticultural Society of London (R.H.S.) (2015 edition) was identified by Eucliean distance in the CIELAB color space, carried out using the farver package in R:

[0044] ‘RO12756’ Cap color: RHS 173D (moderate yellowish pink)

[0045] ‘RO12756’ Stipe color: RHS 159C (pale orange yellow)

[0046] ‘RO12756’ Gill color: RHS 165D (pale yellow)

[0047] ‘RO12756’ sets pins and produces harvestable mushrooms as fast or faster than available brown commercial strains. ‘RO12756’ also exhibits high tray coverage, high yield, a desirably light brown cap color, medium firmness, and thick stipes and cap flesh relative to cap diameter. ‘RO12756’ additionally exhibits thicker cap apex flesh, thicker cap edge flesh, and Percent Harvested Speed faster or as fast as other available brown commercial strains.

[0048] Other characteristics of ‘RO12756’ distinguishable from other varieties are described below (compared to commercial line ‘BR06’ and commercial line ‘B14528’).Comparisons to Commercial Mushroom VarietiesInitial TrialsTray-Level Comparisons

[0049] In further evidence of the distinctness and superiority of mushroom strain ‘RO12756’, provided below are the results of trials conducted across multiple strains' yielded trays of mushrooms. These trials compare timing for trays to reach pinset and harvestable maturity, tray coverage, cap color darkness, cap firmness, cap roundness (visual), yield at each break, and percentage of each break's yield harvested by 17 days post-casing (1st break), 24 days post-casing (2nd break), and 32 days post-casing (3rd break). Characteristics were measured from 74-147 trays of strain ‘RO12756’ mushrooms with corresponding measurements taken of 94-166 trays of strain ‘BR06’ mushrooms (Table 2) and 12-28 trays of strain ‘B14528’ mushrooms (Table 3). Percent Harvest Speed characteristics were measured for 34-36 trials containing strain ‘RO12756’, 33-36 trials containing strain ‘BR06’, and 3-4 trials containing strain ‘B14528’.

[0050] In addition to the comparisons made below, FIGS. 1 and FIGS. 4-5 visually compare variety ‘RO12756’ to other varieties in tray coverage (FIG. 1), cap color (FIG. 4), and qualities regarding cap height and cap flesh thickness (FIG. 5).

[0051] Table 2 below provides initial quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘BR06’. Column 1 lists the tray-level or trial-level characteristics (as described in Table 1), column 2 shows the average value of the characteristic for mushroom strain ‘RO12756’, column 3 shows the standard deviation of the characteristic for mushroom strain ‘RO12756’, column 4 shows the average value of the characteristic for mushroom strain ‘BR06’, column 5 shows the standard deviation of the characteristic for mushroom strain ‘BR06’, and column 6 shows the p-values of two-sample, two-sided t-tests. Bolded p-values indicate p<0.05.

[0052] TABLE 2‘RO12756’‘BR06’CharacteristicAverageSt. Dev.AverageSt. Dev.p-valueBefore First BreakDays to 11.722 0.99912.316 0.8804.139E−05PinsetFirst BreakDays to 1st 17.317 0.75217.598 0.7351.153E−02BreakTray  4.178 0.799 3.935 0.8662.900E−02CoverageCap Color  2.916 0.601 3.872 0.3806.840E−35DarknessCap  3.543 0.519 4.154 0.4254.790E−19FirmnessCap  2.848 0.387 2.984 0.1272.930E−04Roundness (Visual)Percent 24.105%23.66316.868%19.0901.241E−01Harvested SpeedYield (lb / ft2) 2.483 0.701 2.523 1.0176.860E−01Second BreakDays to 2nd 24.824 0.80025.274 0.6751.122E−04BreakTray  4.000 0.611 4.150 0.6056.140E−02CoverageCap Color  3.009 0.427 3.859 0.3499.160E−45DarknessCap  3.509 0.553 3.969 0.4359.200E−12FirmnessCap  2.634 0.484 2.992 0.1541.030E−13Roundness (Visual)Percent 42.447%25.15225.151%23.8111.431E−03Harvested SpeedYield (lb / ft2) 1.606 0.537 1.601 0.6179.340E−01Third BreakPercent 63.200%26.70234.306%32.0352.320E−05Harvested SpeedYield (lb / ft2) 1.038 0.313 0.747 0.3719.180E−13All Breaks CombinedYield (lb / ft2) 5.127 0.792 4.871 0.9189.080E−03

[0053] Table 3 below provides initial quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘B14528’. Column 1 lists the tray-level or trial-level characteristics (as described in Table 1), column 2 shows the average value of the characteristic for mushroom strain ‘RO12756’, column 3 shows the standard deviation of the characteristic for mushroom strain ‘RO12756’, column 4 shows the average value of the characteristic for mushroom strain ‘B14528’, column 5 shows the standard deviation of the characteristic for mushroom strain ‘B14528’, and column 6 shows the p-values of two-sample, two-sided t-tests. Bolded p-values indicate p<0.05.

[0054] TABLE 3‘RO12756’‘B14528’CharacteristicAverageSt. Dev.AverageSt. Dev.p-valueBefore First BreakCompost  4.350 0.737 3.643 1.0266.920E−05ColonizationFirst BreakDays to 1st 17.317 0.75217.000 0.6498.599E−02BreakTray  4.178 0.799 4.000 0.4712.630E−01CoverageCap Color  2.916 0.601 4.000 0.2724.050E−16DarknessCap  3.543 0.519 3.786 0.4182.400E−02FirmnessCap  2.848 0.387 2.929 0.2622.990E−01Roundness (Visual)Percent24.105%23.66019.000%11.2474.392E−01Harvested SpeedSecond BreakDays to 2nd 24.824 0.80024.417 0.6699.848E−02BreakTray  4.000 0.611 3.250 0.5501.150E−06CoverageCap Color  3.009 0.427 3.929 0.3782.670E−19DarknessCap  3.509 0.553 3.857 0.4482.470E−03FirmnessCap  2.634 0.484 2.536 0.5083.430E−01Roundness(Visual)Percent 42.447%25.15239.000%34.4388.380E−01Harvested SpeedIndividual Mushroom-Level Comparisons

[0055] In additional evidence of the distinctness and superiority of mushroom strain ‘RO12756’, provided below is a summary of precise quantitative measurements of individual mushrooms at first and second breaks. These data compare Cap L* Value, Cap a* Value, and Cap b* Values in the International Commission on Illumination (CIELAB) color space, Cap Diameter, Cap Height, Cap Flesh Thickness, Stipe Diameter, Relative Cap Height, Relative Cap Flesh Thickness, and Relative Stipe Diameter (as described in Table 1). Measurements from 390 first break and 100 second break ‘RO12756’ mushrooms were compared to 400 first break and 120 second break ‘BR06’ mushrooms (Table 4) and 150 first break and 80 second break ‘B14528’ mushrooms (Table 5).

[0056] Table 4 below provides initial quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘BR06’. Column 1 lists the mushroom-level characteristics (as described in Table 1), column 2 shows the average value of the characteristic for mushroom strain ‘RO12756’, column 3 shows the standard deviation of the characteristic for mushroom strain ‘RO12756’, column 4 shows the average value of the characteristic for mushroom strain ‘BR06’, column 5 shows the standard deviation of the characteristic for mushroom strain ‘BR06’, and column 6 shows the p-values of two-sample, two-sided t-tests. Bolded p-values indicate p<0.05.

[0057] TABLE 4‘RO12756’‘BR06’CharacteristicAverageSt. Dev.AverageSt. Dev.p-valueFirst BreakCap L* Value 74.237 3.52464.335 5.7075.943E−128(dark-light)Cap a* Value  3.899 1.581 7.605 1.7701.242E−138(green-red)Cap b* Value24.770 2.55928.453 2.4513.905E−76(blue-yellow)Cap Diameter40.571 3.82942.200 4.0057.566E−09Cap Height24.199 1.99425.455 2.0591.797E−17Cap Flesh 13.069 1.39513.130 1.3135.270E−01ThicknessStipe Diameter18.258 2.13919.546 2.5851.130E−13Relative Cap  0.598 0.033 0.605 0.0313.410E−03HeightRelative Cap  0.323 0.028 0.312 0.0263.060E−08Flesh ThicknessRelative  0.451 0.040 0.464 0.0549.166E−05Stipe DiameterSecond BreakCap L* Value 73.318 3.49766.428 4.2741.03E−28(dark-light)Cap a* Value  4.282 1.216 6.359 1.4611.17E−23(green-red)Cap b* Value 24.796 3.16827.600 2.4402.72E−12(blue-yellow)Cap Diameter42.833 5.94643.005 4.0638.00E−01Cap Height24.156 2.11225.313 2.2341.19E−04Cap Flesh 12.359 1.04812.526 1.1632.71E−01ThicknessStipe Diameter16.115 1.90415.878 2.0223.75E−01Relative Cap  0.569 0.053 0.590 0.0356.90E−04HeightRelative Cap  0.293 0.040 0.292 0.0259.06E−01Flesh ThicknessRelative  0.379 0.038 0.370 0.0355.72E−02Stipe Diameter

[0058] Table 5 below provides quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘B14528’. Column 1 lists the mushroom-level characteristics (as described in Table 1), column 2 shows the average value of the characteristic for mushroom strain ‘RO12756’, column 3 shows the standard deviation of the characteristic for mushroom strain ‘RO12756’, column 4 shows the average value of the characteristic for mushroom strain ‘B14528’, column 5 shows the standard deviation of the characteristic for mushroom strain ‘B14528’, and column 6 shows the p-values of two-sample, two-sided t-tests. Bolded p-values indicate p<0.05.

[0059] TABLE 5‘RO12756’‘B14528’CharacteristicAverageSt. Dev.AverageSt. Dev.p-valueFirst BreakCap L* Value74.237 3.52467.579 5.9251.560E−47(dark-light)Cap a* Value  3.899 1.581 5.987 1.8543.850E−34(green-red)Cap b* Value 24.770 2.55927.637 2.3633.510E−29(blue-yellow)Cap Diameter40.571 3.82943.218 3.8276.590E−13Cap Height24.199 1.99426.568 1.8722.430E−33Cap Flesh 13.069 1.39513.057 1.0129.210E−01ThicknessStipe Diameter18.258 2.13917.787 2.0181.790E−02Relative Cap  0.598 0.033 0.616 0.0302.260E−09HeightRelative Cap  0.323 0.028 0.303 0.0243.050E−14Flesh ThicknessRelative Stipe  0.451 0.040 0.413 0.0421.900E−21DiameterSecond BreakCap L* Value 73.318 3.49768.288 5.2178.20E−13(dark-light)Cap a* Value 4.282 1.216 5.256 1.2413.51E−07(green-red)Cap b* Value 24.796 3.16826.285 2.1854.48E−04(blue-yellow)Cap Diameter42.833 5.94641.712 3.4171.35E−01Cap Height24.156 2.11225.062 2.0394.14E−03Cap Flesh 12.359 1.04811.868 1.3707.02E−03ThicknessStipe Diameter16.115 1.90414.725 1.4422.04E−07Relative Cap  0.569 0.053 0.602 0.0334.09E−06HeightRelative Cap  0.293 0.040 0.286 0.0362.14E−01Flesh ThicknessRelative Stipe  0.379 0.038 0.355 0.0441.48E−04DiameterAdditional TrialsTray-Level Comparisons

[0060] Table 6 below provides quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘BR06’.

[0061] The first part of analyzing trait measurements in the additional trials herein included making comparisons between all three of instant strain ‘RO12756’, comparative strain ‘BR06’, and comparative strain ‘B14528’. For these three strains, a Shapiro-Wilk test and a Levene test were performed first to assess if the data satisfy the assumptions of normality and equal variances among groups, respectively, of a standard ANOVA. The Shapiro-Wilk test and Levene test determined that most of these traits satisfied neither assumption. Therefore, a Kruskal-Wallis test (Kruskal, W. H., & Wallis, W. A. (1952). Use of ranks in one-criterion variance analysis. Journal of the American statistical Association, 47(260), 583-621), a non-parametric variation of ANOVA that does not assume normality, equal variances among groups, or equal sample sizes among groups, was performed. Medians and interquartile ranges (IQR) were reported, as these metrics are robust to non-normal distributions. Means are also reported for consistency with normally-distributed traits.

[0062] The Shapiro-Wilk test and Levene test determined that most developmental timing traits (Days to Pinset, Days to 1st Break, and Days to 2nd Break) and yield traits were not normally distributed and variances among groups were not equal. The Kruskal-Wallis test and Dunn post-hoc test (“Dunn's Test”: Dunn, O. J. (1964). Multiple comparisons using rank sums. Technometrics, 6(3), 241-252) were used in these cases. When the Kruskal-Wallis test results were significant based on a threshold of p<0.05. Dunn's test was performed post-hoc to make pairwise strain comparisons (Dunn, 1964). P-values were adjusted using the Bonferroni correction to minimize risk of Type 1 error (false significance). An exception to this trend was Percent Harvested Speed, which was normally distributed and exhibited equal variance for all strains. For assessing Percent Harvested Speed, a standard ANOVA was used and a Tukey Honestly Significant Difference (HSD) test was performed post-hoc for pairwise strain comparisons. Standard deviations multiplied by 100 for consistency alongside percentages (“SD*100”) were reported for this trait. This testing was performed on groups of three strains, but the results show a pairwise subset of the tests for consistency with the initial trials.

[0063] In Table 6, column 1 shows each trait and the strains compared for said trait, column 2 shows the mean value for that strain's measurements of that trait, column 3 shows the median value of the same measurements, column 4 shows the interquartile range (IQR) for the same measurements, column 5 shows the number of trials conducted, and column 6 shows the statistical significance group calculated for that strain for that trait according to Dunn's Test. Traits of particular interest are bolded.

[0064] TABLE 6Num -SignificanceberGroupof(Dunn's VarietyMeanMedianIQRTrialsTest)Before First BreakDays to Pinset‘RO12756’11.411.0 1.008a‘BR06’12.613.0 1.008bFirst BreakDays to 1st Break‘RO12756’17.517.0 1.008a‘BR06’18.518.0 1.008bYield (kg / m2)‘RO12756’11.9112.40 4.888a‘BR06’11.9612.69 9.528aSecond BreakDays to 2nd Break‘RO12756’24.825.0 2.007a‘BR06’25.525.0 1.007bYield (kg / m2)‘RO12756’ 8.54 8.15 2.648a‘BR06’ 7.37 5.96 4.398bThird BreakYield (kg / m2)‘RO12756’ 4.78 4.83 1.868a‘BR06’ 3.66 2.78 2.788bAll Breaks CombinedYield (kg / m2)‘RO12756’25.2924.95 4.158a‘BR06’23.0023.48 5.818bSignificancePercent Harvested Speed Group(measured in First Break)SD * 100(Tukey HSD)‘RO12756’48.2%50.9%16.308b‘BR06’28.6%27.4%20.908a

[0065] Table 7 below provides additional quantitative comparison between mushroom strain ‘RO12756’ and strain ‘B14528’. Column 1 shows the strains compared for each trait, column 2 shows the mean value for that strain's measurements, column 3 shows the median value of the same measurements, column 4 shows either the interquartile range (IQR) or the standard deviation multiplied by 100 for consistency alongside percentages (“SD*100”) for the same measurements, column 5 shows the number of trials conducted, and column 6 shows the statistical significance group calculated for that strain for that trait according to either Dunn's Test or Tukey HSD.

[0066] TABLE 7Num-Significanceber Groupof(Dunn'sCharacteristicMeanMedianIQRTrialsTest)Before First BreakDays to Pinset‘RO12756’11.4011.00 1.008a‘B14528’11.4011.00 1.008aFirst BreakDays to 1st Break‘RO12756’17.5017.00 1.008a‘B14528’17.5017.00 1.008aYield (kg / m2)‘RO12756’11.9112.40 4.888a‘B14528’12.5512.25 5.278aSecond BreakDays to 2nd Break‘RO12756’24.8025.00 2.007a‘B14528’25.0025.00 0.507aYield (kg / m2)‘RO12756’ 8.54 8.15 2.648a‘B14528’ 8.45 8.01 2.398aThird BreakYield (kg / m2)‘RO12756’ 4.78 4.83 1.868a‘B14528’ 5.52 5.57 2.888aAll Breaks CombinedYield (kg / m2)‘RO12756’25.2924.95 4.158a‘B14528’26.5127.10 5.768aPercentSignificanceHarvested SpeedGroup(measured in(Tukey First Break)MeanMedianSD * 100HSD)‘RO12756’48.2%50.9%16.308b‘B14528’53.5%55.7%14.008b

[0067] Table 8 below provides additional quantitative comparison between mushroom strain ‘RO10425’ and strain ‘RO12756’. Column 1 shows the strains compared for each trait, column 2 shows the mean value for that strain's measurements, column 3 shows the median value of the same measurements, column 4 shows either the interquartile range (IQR) or the standard deviation multiplied by 100 for consistency alongside percentages (“SD*100”) for the same measurements, column 5 shows the number of trials conducted, and column 6 shows the statistical significance group calculated for that strain for that trait according to either Dunn's Test or Tukey HSD.

[0068] TABLE 8Num-Significanceber Groupof(Dunn's CharacteristicMeanMedianIQRTrialsTest)Before First BreakDays to Pinset‘RO10425’12.0012.00 2.004a‘RO12756’11.4211.00 1.008aFirst BreakDays to 1st Break‘RO10425’18.0018.001.004a‘RO12756’17.5117.001.008aYield (kg / m2)‘RO10425’10.6410.641.763a‘RO12756’11.9112.404.888aSecond BreakDays to 2nd Break‘RO10425’24.8625.001.754a‘RO12756’24.8425.002.007aYield (kg / m2)‘RO10425’8.748.103.273a‘RO12756’8.548.152.648aThird BreakYield (kg / m2)‘RO10425’3.663.520.783a‘RO12756’4.784.831.868aAll Breaks CombinedYield (kg / m2)‘RO10425’23.0523.144.253a‘RO12756’25.2924.954.158aPercent HarvestedSignificanceSpeed (measured inGroupFirst Break)MeanMedianSD * 100(Tukey HSD)‘RO10425’36.74%34.80%32.653a‘RO12756’48.17%50.88%16.278a

[0069] Table 9 below provides additional quantitative comparison between mushroom strain ‘RO12756’ and strain ‘RO14176’ (U.S. application Ser. No. 19 / 305,681). Column 1 shows the strains compared for each trait, column 2 shows the mean value for that strain's measurements, column 3 shows the median value of the same measurements, column 4 shows either the interquartile range (IQR) or the standard deviation multiplied by 100 for consistency alongside percentages (“SD*100”) for the same measurements, column 5 shows the number of trials conducted, and column 6 shows the statistical significance group calculated for that strain for that trait according to either Dunn's Test or Tukey HSD.

[0070] TABLE 9Num-ber SignificanceofGroupCharacteristicMeanMedianIQRTrials(Dunn's Test)Before First BreakDays to Pinset‘RO12756’11.4211.00 1.008a‘RO14176’11.4412.00 1.007aFirst BreakDays to 1st Break‘RO12756’17.5117.00 1.008a‘RO14176’17.7118.00 1.007aYield (kg / m2)‘RO12756’11.9112.40 4.888a‘RO14176’12.4012.25 4.696aSecond BreakDays to 2nd Break‘RO12756’24.8425.00 2.007a‘RO14176’25.2825.00 2.007aYield (kg / m2)‘RO12756’ 8.54 8.15 2.648a‘RO14176’ 8.45 8.50 3.526aThird BreakYield (kg / m2)‘RO12756’ 4.78 4.83 1.868a‘RO14176’ 4.20 3.71 2.696aAll Breaks CombinedYield (kg / m2)‘RO12756’25.2924.95 4.158a‘RO14176’25.0523.88 3.666aPercent HarvestedSignificanceSpeed (measured inGroupFirst Break)MeanMedianSD * 100(Tukey HSD)‘RO12756’48.17%50.88%16.278a‘RO14176’38.65%39.88%13.105aIndividual Mushroom-Level Comparisons

[0071] In additional evidence of the distinctness and superiority of mushroom strain ‘RO12756’, provided below is a summary of precise quantitative measurements of individual mushrooms at first and second breaks. These data compare Cap L* Value, Cap a* Value, and Cap b* Values in the International Commission on Illumination (CIELAB) color space, Cap Apex Flesh Thickness, Cap Edge Flesh Thickness, and Cap Roundness (Calculated) (as described in Table 1). Measurements were taken for hundreds of mushrooms per trait, noted as sample size (n) in Tables 10 and 11.

[0072] Table 10 below provides additional quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘BR06’. Column 1 shows each trait and the strains compared for said trait, column 2 shows the mean value for that strain's measurements of that trait, column 3 shows the median value of the same measurements, column 4 shows the interquartile range (IQR) for the same measurements, column 5 shows the number of mushrooms assessed (n), and column 6 shows the statistical significance group calculated for that strain for that trait according to Dunn's Test.

[0073] TABLE 10Number Significanceof Mush-Grouprooms(Dunn's VarietyMeanMedianIQR(n)Test)First BreakCap L* Value (dark-light)‘RO12756’72.1272.294.81580a‘BR06’63.3062.749.83797bCap a* Value (green-red)‘RO12756’5.625.661.88580a‘BR06’8.678.962.58787bCap b* Value (blue-yellow)‘RO12756’26.9827.064.11580a‘BR06’28.7229.043.17776bCap Apex Flesh Thickness‘RO12756’0.550.550.06575a‘BR06’0.520.520.05541bCap Edge Flesh Thickness‘RO12756’0.220.230.10569a‘BR06’0.210.210.09537aCap Roundness (Calculated)‘RO12756’0.610.610.04579a‘BR06’0.610.610.04541aSecond BreakCap L* Value (dark-light)‘RO12756’73.0272.874.35200a‘BR06’65.6865.495.90340bCap a* Value (green-red)‘RO12756’4.844.951.64200a‘BR06’7.217.312.42340bCap b* Value (blue-yellow)‘RO12756’26.5526.833.24200a‘BR06’28.1528.492.86340bCap Apex Flesh Thickness‘RO12756’0.540.540.04198a‘BR06’0.510.520.07362bCap Edge Flesh Thickness‘RO12756’0.270.270.10199a‘BR06’0.230.230.09362bCap Roundness (Calculated)‘RO12756’0.560.560.05199a‘BR06’0.600.590.06363b

[0074] Table 11 shows additional quantitative comparisons between mushroom strain ‘RO12756’ and strain ‘B14528’. Column 1 lists the mushroom-level characteristics (as described in Table 1) and each strain, column 2 shows the mean value of the characteristic, column 3 shows the median value of the characteristic, column 4 shows the interquartile range (IQR) for the characteristic, column 5 shows the number of mushrooms assessed (n), and column 6 shows the statistical significance group calculated according to Dunn's Test.

[0075] TABLE 11Number Significanceof Mush-Grouprooms(Dunn's VarietyMeanMedianIQR(n)Test)First BreakCap L* Value (dark-light)‘RO12756’72.1272.294.81580a‘B14528’64.0664.136.56340bCap a* Value (green-red)‘RO12756’5.625.661.88580a‘B14528’8.268.312.13340cCap b* Value (blue-yellow)‘RO12756’26.9827.064.11580a‘B14528’30.3130.163.39340cCap Roundness (Calculated)‘RO12756’0.610.610.04579a‘B14528’0.610.610.05389aCap Apex Flesh Thickness‘RO12756’0.550.550.06575a‘B14528’0.470.460.06387cCap Edge Flesh Thickness‘RO12756’0.220.230.10569a‘B14528’0.220.220.08387aSecond BreakCap L* Value (dark-light)‘RO12756’73.0272.874.35200a‘B14528’68.5669.026.54170cCap a* Value (green-red)‘RO12756’4.844.951.64200a‘B14528’6.095.962.05170cCap b* Value (blue-yellow)‘RO12756’26.5526.833.24200a‘B14528’27.3727.783.22170cCap Roundness (Calculated)‘RO12756’0.560.560.05199a‘B14528’0.600.600.05198bCap Apex Flesh Thickness‘RO12756’0.540.540.04198a‘B14528’0.460.460.05198cCap Edge Flesh Thickness‘RO12756’0.270.270.10199a‘B14528’0.230.230.06198b

[0076] One of the advantages of the strain ‘RO12756’ is its incompatibility with other commercial strains. Table 12 below provides results of compatibility tests between strain ‘RO12756’ and commercial strain ‘BR06’, commercial strain ‘B14528’, or related strain ‘RO10425’. These results indicate that strain ‘RO12756’ is incompatible with strain ‘BR06’, strain ‘B14528’, and related strain ‘RO10425’. This was true regardless of which tested strain served as the compost variety and which tested strain served as the casing variety.

[0077] TABLE 12StrainsMeanMedian(compost variety / lb / kg / lb / kg / casing variety)ft2m2ft2m2‘RO12756’ / 2.6212.79 2.6112.74‘RO12756’‘RO10425’ / 2.7113.23 2.8613.94‘RO10425’‘BR06’ / 2.6212.79 2.4611.99‘BR06’‘B14528’ / 2.9314.31 2.9214.23‘B14528’‘RO12756’ / 0.703.420.623.03‘BR06’,‘BR06’ / ‘RO12756’‘RO12756’ / 0.683.320.602.91‘B14528’,‘B14528’ / ‘RO12756’‘RO12756’ / 0.914.441.045.08‘RO10425’,‘RO10425’ / ‘RO12756’StrainsSDSigni-(compost variety / lb / kg / ficancecasing variety)ft2m2TrialsGroup‘RO12756’ / 0.412.003a‘RO12756’‘RO10425’ / 0.502.443a‘RO10425’‘BR06’ / 1.155.612a‘BR06’‘B14528’ / 0.341.661a‘B14528’‘RO12756’ / 0.251.222b‘BR06’,‘BR06’ / ‘RO12756’‘RO12756’ / 0.432.101b‘B14528’,‘B14528’ / ‘RO12756’‘RO12756’ / 0.351.711b‘RO10425’,‘RO10425’ / ‘RO12756’

[0078] Table 13 below shows quantitative comparisons between mushroom strain ‘RO10425’ and strain ‘RO12756’. Column 1 shows each trait and the strains compared for said trait, column 2 shows the mean value for that strain's measurements of that trait, column 3 shows the median value of the characteristic, column 4 shows the interquartile range (IQR) for the characteristic, column 5 shows the number of mushrooms assessed (n), and column 6 shows the statistical significance group calculated according to Dunn's Test.

[0079] TABLE 13Number Significanceof Mush-Grouprooms(Dunn's VarietyMeanMedianIQR(n)Test)First BreakCap L* Value (dark-light)‘RO10425’65.6665.227.34209a‘RO12756’72.1272.294.81580bCap a* Value (green-red)‘RO10425’7.357.431.97209a‘RO12756’5.625.661.88580bCap b* Value (blue-yellow)‘RO10425’28.4328.583.1209a‘RO12756’26.9827.064.11580bCap Apex Flesh Thickness‘RO10425’0.550.550.04166a‘RO12756’0.550.550.06575aCap Edge Flesh Thickness‘RO10425’0.300.310.05166a‘RO12756’0.220.230.10569bCap Roundness (Calculated)‘RO10425’0.640.630.05166a‘RO12756’0.610.610.04579bSecond BreakCap L* Value (dark-light)‘RO10425’68.8269.478.20157a‘RO12756’73.0272.874.35200bCap a* Value (green-red)‘RO10425’6.156.262.55157a‘RO12756’4.844.951.64200bCap b* Value (blue-yellow)‘RO10425’27.6927.462.54157a‘RO12756’26.5526.833.24200bCap Apex Flesh Thickness‘RO10425’0.550.550.04157a‘RO12756’0.540.540.04198aCap Edge Flesh Thickness‘RO10425’0.320.320.05157a‘RO12756’0.270.270.10199bCap Roundness (Calculated)‘RO10425’0.610.610.05157a‘RO12756’0.560.560.05199b

[0080] Table 14 below shows quantitative comparisons between mushroom strain ‘RO14176’ and strain ‘RO12756’. Column 1 shows each trait and the strains compared for said trait, column 2 shows the mean value for that strain's measurements of that trait, column 3 shows the median value of the characteristic, column 4 shows the interquartile range (IQR) for the characteristic, column 5 shows the number of mushrooms assessed (n), and column 6 shows the statistical significance group calculated according to Dunn's Test.

[0081] TABLE 14Number Significanceof Mush-Grouprooms(Dunn's VarietyMeanMedianIQR(n)Test)First BreakCap L* Value (dark-light)‘RO12756’72.1272.294.81580b‘RO14176’65.7665.825.82469aCap a* Value (green-red)‘RO12756’5.625.661.88580b‘RO14176’7.457.471.58469aCap b* Value (blue-yellow)‘RO12756’26.9827.064.11580b‘RO14176’28.1728.032.64469aCap Apex Flesh Thickness‘RO12756’0.550.550.06575a‘RO14176’0.540.540.06453bCap Edge Flesh Thickness‘RO12756’0.220.230.10569b‘RO14176’0.270.260.07449cCap Roundness (Calculated)‘RO12756’0.610.610.04579b‘RO14176’0.640.640.05455cSecond BreakCap L* Value (dark-light)‘RO12756’73.0272.874.35200b‘RO14176’68.9268.866.39263aCap a* Value (green-red)‘RO12756’4.844.951.64200b‘RO14176’6.376.431.60261aCap b* Value (blue-yellow)‘RO12756’26.5526.833.24200b‘RO14176’27.3627.352.11261aCap Apex Flesh Thickness‘RO12756’0.540.540.04198a‘RO14176’0.540.540.04235aCap Edge Flesh Thickness‘RO12756’0.270.270.10199b‘RO14176’0.310.310.06235cCap Roundness (Calculated)‘RO12756’0.560.560.05199b‘RO14176’0.610.600.05235a

Claims

1. A new and distinct variety of Agaricus bisporus Imbach mushroom named ‘RO12756’, as shown and described herein.

Images