Molecular rheostat for cofactor balance
The molecular rheostat system in metabolic pathways addresses cofactor balance issues by dynamically switching between pathways, enhancing chemical production efficiency and yield in in vitro systems.
USRE50957E1Active Publication Date: 2026-07-21RGT UNIV OF CALIFORNIA
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Patent Information
- Authority / Receiving Office
- US · United States
- Patent Type
- Patents(United States)
- Current Assignee / Owner
- RGT UNIV OF CALIFORNIA
- Filing Date
- 2022-08-26
- Publication Date
- 2026-07-21
AI Technical Summary
Technical Problem
Existing in vitro metabolic systems face challenges in maintaining cofactor balance and flux due to the second law of thermodynamics, leading to inefficiencies and limited duration of chemical production.
Method used
A recombinant metabolic pathway with a molecular rheostat system that switches between cofactor pathways based on availability, using enzymes like NADP+/NADPH and Pi to regulate ATP production and consumption, allowing for self-regulating cofactor balance.
Benefits of technology
The system maintains cofactor balance without strict stoichiometric matching, enabling longer-lasting and more efficient production of chemicals like isobutanol, achieving higher yields compared to traditional stoichiometric pathways.
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Abstract
The disclosure provides a metabolic pathway for producing a metabolite, the metabolic pathway having a co-factor regulatory system for cofactor utilization in the metabolic pathway.
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