PURIFICATION OF RECOMBINANTLY PRODUCED POLYPEPTIDES.

Description

The present invention relates generally to processes for the production of strongly glycosylated recombinant proteins (for example, mucins and mucin-like proteins such as lubricin). The processes comprise cultivating mammalian cells capable of producing a glycoprotein in a liquid medium in a system comprising one or more bioreactors, concentrating, purifying, and formulating the glycoprotein. The purification comprises one or more chromatography steps, an endonuclease step, and at least one viral inactivation step. In certain aspects, the invention relates to pharmaceutical compositions comprising purified recombinant human lubricin, and to methods of treating a subject in need thereof.