Methods for preparing plant-based bioactive agents to enhance photosynthesis in plants.

Abstract

The invention relates to a method for preparing a plant-based bioactive agent to enhance photosynthesis in plants, including the preparation of animal amino acids by hydrolyzing feather meal using proteolytic enzymes; and the mixing and liquefaction of the prepared animal amino acids and reactive raw materials including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid, and water. Thus, the method can not only enhance photosynthesis in plants, but also prevent agricultural damage caused by climate change.

Description

Method for producing a plant physiological activator for promoting photosynthesis in plants

[0001] The present invention (Disclosure) relates to a method for producing a plant physiological activator for promoting photosynthesis in plants, and more specifically, to a method for producing a plant physiological activator for promoting photosynthesis in plants, which comprises hydrolyzing protein using a protease to produce animal amino acids, mixing the produced animal amino acids with a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate and an organic acid, and then liquefying the mixture to produce a plant physiological activator, thereby promoting photosynthesis in plants and preventing agricultural damage caused by climate change in advance.

[0002] This section provides background information related to the present disclosure which is not necessarily prior art.

[0003] The world is experiencing abnormal weather due to global warming, and El Niño, La Niña, and typhoons are causing a lot of agricultural damage. In Korea, rice production in 2020 was the lowest ever at 3.5 million tons due to the late monsoon season and typhoons, and fruit tree harvests also decreased due to cold damage and fruit drop. This is because the photosynthetic efficiency is lowered as the amount of sunlight is insufficient due to climate change, which reduces the production of assimilated products necessary for growth.

[0004] Meanwhile, fertilizers used in crop cultivation are generally composed of compound fertilizers based on N (nitrogen), P (phosphorus), and K (potassium), trace element fertilizers, and organic fertilizers. Plant physiological activators are being proposed as an alternative to overcome the problems of reduced crop yield and quality due to climate change.

[0005] These plant physiological activators refer to substances (seaweed extracts, animal / plant amino acids, humic acids, etc.) or microorganisms that activate the physiological functions of plants, thereby enhancing resistance to abiotic stress (environmental factors such as drought, high / low temperature damage, and cold damage) and improving the productivity and quality of crops.

[0006] Among these, animal amino acids, which are used in large quantities, contain various types of amino acids such as glycine, methionine, alanine, and glutamic acid, which are obtained when the protein of animal hair and feathers is completely hydrolyzed. Amino acids activate various physiological functions in plants, such as root development, growth promotion, improvement of sugar content and coloring of fruits, increase of disease resistance, and stress prevention. In particular, glutamic acid is known to help leaf activity, such as promoting photosynthesis.

[0007] Additionally, most amino acids used as nutrients for plants are manufactured through chemical hydrolysis using strong acids such as hydrochloric acid (HCl) and sulfuric acid (H2SO4). Chemically decomposed amino acids are mostly in the D form, which makes them less active in organisms. Furthermore, among the 18 amino acids, cyclic amino acids are known to be destroyed, and when hydrochloric acid is used, they remain in the ammonium chloride state, which can cause phytotoxicity to crops.

[0008] In addition, hydrochloric acid and sulfuric acid used in the decomposition process are registered as hazardous chemicals and substances under priority management, so great care must be taken when using them. Therefore, a method that can replace chemical decomposition using strong acids is needed.

[0009] Meanwhile, organic acids are closely related to the growth of plant roots. The area affected by the roots of living plants in the soil is called the rhizosphere, and the area is approximately 2 mm from the surface of the roots. Roots secrete organic acids to make nutrients available and are known to affect the formation of soil microbial communities. Soil microorganisms decompose organic matter in the soil to improve the soil and create an environment conducive to root development, so they can be seen as interacting with each other.

[0010] In addition, existing products for root development and root zone formation are based on microbial preparations that utilize organic acids produced by microorganisms, but they have the disadvantage of a short distribution period due to low organic acid content and weak microbial activity. In addition, when organic acids are directly mixed, they contain only a small amount of 1-3% due to limitations in manufacturing technology.

[0011] As mentioned above, as global warming caused abnormal climates worldwide, agricultural damage occurred, and attempts were made to overcome this through seed improvement, pesticides, and chemical fertilizers. However, as these reached their limits, the concept of biostimulants (plant physiological activators) was formed as an alternative.

[0012] These biostimulants are plant physiological activators, and refer to substances (seaweed extracts, amino acids, humic acids) or microorganisms that activate the physiological functions of plants, thereby enhancing resistance to abiotic stress and improving the productivity and quality of crops.

[0013] Currently, biostimulant associations have been established in the United States, Europe, Japan, and other countries to promote the enactment of related laws, and in February 2020, a biostimulant law was enacted in India.

[0014] While interest in biostimulants is growing worldwide, related research and development is insufficient in Korea, and research and development on plant biostimulants are necessary to overcome agricultural damage caused by climate change.

[0015] The present invention (Disclosure) relates to a method for producing a plant physiological activator for promoting photosynthesis in plants, and specifically, to a method for producing a plant physiological activator for promoting photosynthesis in plants, which comprises hydrolyzing protein using a protease to produce animal amino acids, mixing the produced animal amino acids with a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate and an organic acid, and then liquefying the mixture to produce a plant physiological activator, thereby promoting photosynthesis in plants and preventing agricultural damage caused by climate change in advance.

[0016] This section provides a general summary of the disclosure and is not a comprehensive disclosure of its full scope or all of its features.

[0017] In order to solve the above problem, a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect among several aspects describing the present invention comprises the steps of: producing animal amino acids by hydrolyzing feather powder using a protease; and liquefying by mixing the produced animal amino acids with a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate, an organic acid, and water.

[0018] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the step of producing the animal amino acid may include a step of sterilizing the feather powder by high pressure steam; a step of adding the proteolytic enzyme to the feather powder; a step of reacting the feather powder and the proteolytic enzyme; a step of filtering after the reacting step; and a step of powdering by freeze drying after the filtering step.

[0019] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the step of sterilizing the feather powder with high pressure steam can be performed in a pressure range of 2.8-3.2 bar, a temperature range of 135-145°C, and a time range of 25-35 minutes.

[0020] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the reacting step can be performed at a temperature range of 45-55°C and a time range of 2.5-3.5 days.

[0021] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the filtering step can be performed according to a particle size range of 150-250 mesh.

[0022] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the step of mixing and liquefying the reaction raw materials may include the steps of adding the reaction raw materials including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid and water to the animal amino acid, and further adding manganese sulfate, boric acid, sodium molybdate, chelated iron and zinc sulfate; stirring and reacting the animal amino acid and the reaction raw materials; precipitating after the stirring and reacting step; and filtering and aging after the precipitating step.

[0023] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the stirring and reacting steps can be performed at a temperature range of 50-60°C, a time range of 5.5-6.5 hours, and a rotation speed range of 550-650 rpm.

[0024] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the precipitating step can be performed over a time range of 17-19 hours.

[0025] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the step of aging after filtering may be performed according to a particle size range of 250-350 mesh, and then aging may be performed according to a time range of 46-50 hours.

[0026] In a method for producing a plant physiological activator for promoting photosynthesis of a plant according to one aspect of the invention, the plant physiological activator can be diluted 900-1100 times and sprayed as foliar fertilizer during the seedling stage or early growth stage of the plant.

[0027] According to the present invention, a method for producing a plant physiological activator for promoting photosynthesis in plants is provided, specifically, by hydrolyzing protein using a protease to produce animal amino acids, mixing the produced animal amino acids with a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate and an organic acid, and then liquefying the mixture to produce a plant physiological activator, thereby not only promoting photosynthesis in plants but also preventing agricultural damage caused by climate change in advance.

[0028] In addition, according to the present invention, a plant physiological activator manufactured by a composite technology using a reaction raw material including animal amino acids, potassium nitrate, calcium nitrate, magnesium nitrate, and organic acid is more environmentally friendly than conventional manufacturing methods, and when used as a plant fertilizer, there is no damage caused by harmful chemical components, and the production of assimilates can increase due to an increase in chlorophyll in the plant body, and accordingly, it can help plants grow stably in abnormal climates such as increased rainfall and insufficient day length due to climate change.

[0029] Figure 1 is a flow chart showing a process for manufacturing a plant physiological activator for promoting photosynthesis of plants according to an embodiment of the present invention.

[0030] Figures 2 and 3 are flowcharts showing detailed processes for manufacturing a plant physiological activator according to an embodiment of the present invention.

[0031] Hereinafter, an embodiment of a method for manufacturing a plant physiological activator for promoting photosynthesis of plants according to the present invention will be described in detail with reference to the drawings.

[0032] However, it is to be understood that the intrinsic technical idea of ​​the present invention is not limited in its possible implementation by the embodiments described below, and encompasses a range that can be easily proposed by a person skilled in the art by replacing or changing the embodiments described below based on the intrinsic technical idea of ​​the present invention.

[0033] In addition, the terms used below have been selected for convenience of explanation, and therefore, in understanding the intrinsic technical idea of ​​the present invention, they should be appropriately interpreted in a meaning that is consistent with the technical idea of ​​the present invention, without being limited to the dictionary meaning.

[0034] Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings.

[0035] FIG. 1 is a flow chart showing a process for manufacturing a plant physiological activator for promoting photosynthesis of plants according to an embodiment of the present invention, and FIGS. 2 and 3 are flow charts showing detailed processes for manufacturing a plant physiological activator according to an embodiment of the present invention.

[0036] Referring to Figures 1 to 3, animal amino acids can be produced by hydrolyzing feather powder using a protein-decomposing enzyme (step 110).

[0037] Here, the proteolytic enzyme may include at least one selected from pepsin, rennin, trypsin, chymotrypsin, erepsin, enterokinase, papain, bromelain, ficin, actinidin, and elastase.

[0038] Meanwhile, in the present invention, it has been described that protein is hydrolyzed using a protease, but it is of course possible to hydrolyze protein using a microorganism that produces a protease instead of a protease, and examples thereof may include bacteria (e.g., Bacillus, etc.), molds (e.g., Aspergillus, Rhizopus, etc.), etc.

[0039] If we describe the specific process of the above step (110), the feather powder can be sterilized by high-pressure steam (step 111).

[0040] Here, down powder refers to the down remaining after slaughtering poultry, processed using steam pressure, dried, and ground. It has a very high crude protein content of 76-87%, and very low contents of crude fiber, calcium, and phosphorus. In order to improve the protein value, it can be used by heating and decomposing it at high temperature and high pressure to break down hydrogen bonds and sulfur bonds and make it into a peptide form.

[0041] In the step (111) of high-pressure steam sterilization of the feather powder as described above, high-pressure steam sterilization can be performed according to the pressure range of 2.8-3.2 bar, the temperature range of 135-145 ℃, and the time range of 25-35 minutes. After putting the feather powder into the high-pressure steam sterilizer (autoclave), the water inside the sealed container is exposed to high temperature while the inside and outside are sealed, thereby generating steam and increasing the pressure and temperature inside the container to the pressure range of 2.8-3.2 bar and the temperature range of 135-145 ℃, and then the pressure and temperature are maintained and processed according to the time range of 25-35 minutes, thereby sterilizing bacteria existing in the feather powder.

[0042] And, a protein-decomposing enzyme can be added to the feather powder (step 112).

[0043] In the above-described injection step (112), after the high-pressure steam sterilized feather powder is injected into the reaction vessel, a protein-decomposing enzyme can be injected into the feather powder in the reaction vessel for hydrolysis.

[0044] Next, the umami powder and protein-decomposing enzyme can be reacted (step 113).

[0045] In the above-described reaction step (113), the reaction can be carried out at a temperature range of 45-55°C and over a time range of 2.5-3.5 days. The reaction can be carried out over a time range of 2.5-3.5 days while maintaining the internal temperature of the reaction vessel into which the feather powder and protein-decomposing enzyme are added at a temperature range of 45-55°C.

[0046] After the above reaction step (113), filtering can be performed according to the preset particle size range (step 114).

[0047] In the above-described filtering step (114), the filtering can be performed according to a particle size range of 150-250 mesh. The reaction solution in which the feather powder and the protein-decomposing enzyme are reacted can be filtered by passing it through a membrane filter having a particle size range of 150-250 mesh.

[0048] After the above filtering step (114), it can be freeze-dried and powdered (step 115).

[0049] In the above-described powdering step (115), the filtered filtrate is frozen, the ice is removed by sublimation, and then the temperature is increased to remove moisture, thereby freeze-drying, and the powder is ground into a powder of a preset particle size range (e.g., 250-350 mesh, etc.) to uniformly powderize the filtrate.

[0050] Next, by mixing and liquefying the animal amino acid produced in the above step (110) and the reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid, and water, a plant physiological activator for promoting photosynthesis of plants can be produced (step 120).

[0051] Here, animal amino acids can be mixed in an amount of 30-40 parts by weight based on 100 parts by weight of the reaction raw material.

[0052] As for the specific process of the above step (120), a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid and water is added to animal amino acid, and manganese sulfate, boric acid, sodium molybdate, chelated iron and zinc sulfate may be further added (step 121).

[0053] Here, the reaction raw material mixed with the animal amino acid may include, based on 100 parts by weight of the reaction raw material, 2.2-2.3 parts by weight of potassium nitrate, 18-20 parts by weight of calcium nitrate, 28-30 parts by weight of magnesium nitrate, 10-11 parts by weight of organic acid, 36-40 parts by weight of water, 0.03-0.05 parts by weight of manganese sulfate, 0.7-0.8 parts by weight of boric acid, 0.02-0.04 parts by weight of sodium molybdate, 0.1-0.3 parts by weight of chelated iron, and 0.03-0.05 parts by weight of zinc sulfate.

[0054] In addition, potassium nitrate contains 13.8% pure nitrogen and 46.85% potassium oxide, and not only does it contain two of the three major nutrients, nitrogen (N), phosphorus (P), and potassium (K), but it also has good water solubility and is advantageous in that it does not form residues of harmful substances such as chloride ions and sulfate ions compared to potassium chloride and potassium sulfate, and is known to be effective in slowing down soil salt accumulation.

[0055] In addition, calcium nitrate has a nitrogen and calcium ratio of 1:3, which promotes calcium absorption and prevents nitrogen deficiency. It is known to be effective in promoting the development of plant meristems and root growth points, strengthening plant cell membranes, blocking harmful substances from entering cells, and removing waste products accumulated in the body.

[0056] Meanwhile, magnesium nitrate is known to be able to supply crops with water-soluble nitrate nitrogen and water-soluble magnesium, quickly replenish magnesium to crops, increase chlorophyll content in plants, promote photosynthesis in crops, and be quickly absorbed by plants.

[0057] And, the organic acid includes at least one selected from, for example, ascorbic acid, carboxylic acid, gluconic acid, succinic acid, glutamic acid, fumaric acid, acetic acid, palmitic acid, tartaric acid, lactic acid, malic acid, succinic acid, tartaric acid, citric acid, acetic acid, oxalic acid, and humic acid, and is known to be created through various metabolic processes by assimilation products produced by photosynthesis, and when nitrogen fertilizer is applied, the absorbed nitrogen component can combine with the organic acid to synthesize amino acids, and has a disease suppression effect on various crops, and when applied to soil where phosphoric acid has accumulated, it can solubilize phosphoric acid and promote the absorption of phosphoric acid.

[0058] In addition, manganese sulfate is known to promote the action of oxidative enzymes and is involved in chlorophyll production and photosynthesis, nitrogen metabolism and carbon assimilation, and vitamin C formation, and is known to promote IAA oxidation by activating IAA oxidase.

[0059] Meanwhile, boric acid is mainly composed of water-soluble boron and is slightly acidic. When the pH of the soil becomes acidic, it changes to an effective form and its availability increases. It is known to be necessary for the vegetative growth period when crops are actively growing as it is an element that is difficult to move within the plant body.

[0060] Additionally, sodium molybdate is known to contain molybdenum and sodium, increasing chlorophyll content, improving photosynthetic ability, promoting plant growth, and improving storage properties.

[0061] In addition, chelated iron is chelated iron, which can suppress the effects of acidity due to its electrical properties, binding with other components, and insolubility, and is known to increase the absorption rate of iron even at high acidity and to be effective in restoring photosynthetic activity of plants.

[0062] Meanwhile, zinc sulfate contains sulfur and zinc, which are nutrients for plants, and thus affects the growth and quality of crops from the early stages of growth. It is known to produce auxin, a plant growth regulator, and promote enzyme activation and chlorophyll synthesis.

[0063] Next, the animal amino acid and the reaction raw material can be stirred and reacted (step 122).

[0064] In the stirring and reacting step (122) as described above, stirring and reacting can be performed in a temperature range of 50-60°C, a time range of 5.5-6.5 hours, and a rotation speed range of 550-650 rpm. After the animal amino acid and the reaction raw material are put into the stirrer, the internal temperature is raised to a temperature range of 50-60°C and maintained, and stirring and reacting can be performed in a time range of 5.5-6.5 hours while rotating the stirring blade in a rotation speed range of 550-650 rpm.

[0065] After the above stirring and reacting step (122), precipitation can be performed (step 123).

[0066] In the precipitation step (123) as described above, precipitation can be performed over a time range of 17-19 hours. The reaction solution obtained through stirring and reaction can be maintained at room temperature (e.g., 15-25°C) for 17-19 hours to precipitate the solid substances remaining after stirring and reaction.

[0067] After the above-mentioned precipitation step (123), a plant physiological activator for promoting photosynthesis of plants can be manufactured by filtering according to a preset particle size range and then maturing (step 124).

[0068] In the aging step (124) after the above filtration, the filtration can be performed according to a particle size range of 250-350 mesh, and then the aging can be performed according to a time range of 46-50 hours. The precipitate in which the remaining solid substances are precipitated can be filtered by passing it through a membrane filter having a particle size range of 250-350 mesh, and the filtrate can be aged according to a time range of 46-50 hours at room temperature (e.g., 15-25°C).

[0069] The liquid plant bioactive agent manufactured as described above can be packaged and sold according to the required weight.

[0070] Meanwhile, the plant physiological activator for promoting photosynthesis of plants manufactured through the process described above can be diluted 500-1000 times and sprayed as foliar fertilizer during the seedling or early growth stage of plants. 10-20 ml of the manufactured plant physiological activator can be diluted by mixing it in approximately 10 liters of water and then sprayed.

[0071] Next, the test results (Mokwon University Microbial Ecological Resources Research Center - Fertilizer Testing and Research Institute) when the plant physiological activator manufactured as described above was diluted 500 times and sprayed as foliar fertilizer after transplanting grass, radish, and cabbage are explained. As a result of the test measuring leaf length, root length, and chlorophyll content, as shown in Table 1 below, it can be confirmed that the plant physiological activator according to the example of the present invention is effective in promoting growth and increasing chlorophyll content compared to the untreated and control groups.

[0072] Crop name Test plot Leaf length (cm) Root length (cm) Chlorophyll content Red lettuce No treatment 1.136.9626.75 Control group 2.379.3630.85 Physiologically active agent of the present invention 3.536.5433.74 Green lettuce No treatment 1.028.3814.77 Control group 2.2411.9419.80 Physiologically active agent of the present invention 3.7213.9420.87 Grass No treatment 10.6624.5838.62 Control group 12.5825.0838.68 Physiologically active agent of the present invention 14.5829.2038.82 No No treatment 1.957.5423.60 Control group 3.0810.0424.85 Physiologically active agent of the present invention Physiologically active agent 4.3511.0826.90 Cabbage untreated 1.577.3420.77 Control group 2.409.3621.42 Physiologically active agent of the present invention 3.5610.521.68

[0073] In addition, it can be confirmed that the plant physiological activator for promoting photosynthesis of plants manufactured as described above contains all of the various types of amino acids contained in the amino acid powder as shown in Table 2 below.

[0074] amino acid 1. amino acid powder (umole / g) 2. green charcoal (umole / ml) histidine 2.0160.044 serine 18.4410.159 arginine 12.1170.086 glycine 5.4320.087 glutamic acid4.9350.075threonine6.6510.091alanine13.6280.125proline5.3340.041cystein0.1770.300lysine2.3620.047tyrosi ne5.6750.071methionine1.6370.003valine0.9990.015isoleucine2.3660.011leucine4.7240.026phenylalanine5.5210.030

[0075] Therefore, according to an embodiment of the present invention, a method for producing a plant physiological activator for promoting photosynthesis of plants is provided, specifically, by hydrolyzing protein using a protease to produce animal amino acids, mixing the produced animal amino acids with a reaction raw material including potassium nitrate, calcium nitrate, magnesium nitrate, and an organic acid, and then liquefying the mixture to produce a plant physiological activator, thereby not only promoting photosynthesis of plants but also preventing agricultural damage caused by climate change in advance.

[0076] In addition, according to an embodiment of the present invention, a plant physiological activator manufactured by a composite technology using a reaction raw material including animal amino acid, potassium nitrate, calcium nitrate, magnesium nitrate, and organic acid is more environmentally friendly than a conventional manufacturing method, and when used as a plant fertilizer, there is no damage caused by harmful chemical components, and the production of assimilates can increase due to an increase in chlorophyll in the plant body, and accordingly, it can help plants grow stably in abnormal climates such as increased rainfall and insufficient day length due to climate change.

Claims

1. A step of producing animal amino acids by hydrolyzing the feather meal using a proteolytic enzyme; and A step of mixing and liquefying the above-mentioned manufactured animal amino acid and reaction raw materials including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid and water; The step of manufacturing the above animal amino acid is: It includes a step of sterilizing the feather powder with high pressure steam; a step of adding the protein-decomposing enzyme to the feather powder; a step of reacting the feather powder and the protein-decomposing enzyme; a step of filtering after the reacting step; and a step of powdering by freeze-drying after the filtering step. The step of sterilizing the above-mentioned powder with high pressure steam is performed according to the pressure range of 2.8-3.2 bar, the temperature range of 135-145 ℃, and the time range of 25-35 minutes. The above reaction step is performed at a temperature range of 45-55°C and a time range of 2.5-3.5 days. The above filtering step is performed according to the particle size range of 150-250 mesh. The above-mentioned powdering step is a method for producing a plant physiological activator for promoting photosynthesis of plants by grinding it into a powder of 250-350 mesh and powdering it.

2. In claim 1, The step of mixing and liquefying the above reaction raw materials is as follows: A step of adding the above reaction raw materials including potassium nitrate, calcium nitrate, magnesium nitrate, organic acid and water to the above animal amino acid, and further adding manganese sulfate, boric acid, sodium molybdate, chelated iron and zinc sulfate; A step of stirring and reacting the above animal amino acids and reaction raw materials; A precipitation step after the stirring and reacting step; and A method for producing a plant physiological activator for promoting photosynthesis of plants, comprising a step of filtering and maturing after the above-mentioned precipitating step.

3. In claim 2, A method for producing a plant physiological activator for promoting photosynthesis of plants, wherein the stirring and reacting steps are performed at a temperature range of 50-60°C, a time range of 5.5-6.5 hours, and a rotation speed range of 550-650 rpm.

4. In claim 3, A method for producing a plant physiological activator for promoting photosynthesis of plants, wherein the above-mentioned precipitating step is performed over a time range of 17-19 hours.

5. In claim 4, A method for producing a plant physiological activator for promoting photosynthesis of plants, wherein the step of aging after filtering is performed according to a particle size range of 250-350 mesh, and then aging is performed according to a time range of 46-50 hours.

6. In claim 5, A method for producing a plant physiological activator for promoting photosynthesis in plants, wherein the above plant physiological activator is diluted 900-1100 times and sprayed as foliar fertilizer during the seedling stage or early growth stage of plants.

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