Methods for isolating compounds with SARS-CoV-2 antiviral activity from the plant *V. rotundifolia L.*.

Abstract

The invention relates to a method for isolating compounds with SARS-CoV-2 antiviral activity from the Vitex rotundifolia L. plant, comprising the following steps: i) producing n-hexane-ethyl acetate (EtOAc) extract (also known as HEA extract) from Vitex rotundifolia powder; ii) extracting the HEA extract by Sephadex silica gel column chromatography and methanol elution solvent to obtain four fractions HEA1, HEA2, HEA3, and HEA4; iii) extracting fraction HEA2 by silica gel column chromatography and an elution solvent mixture of n-hexane:EtOAc:methanol (MeOH); and obtaining four fractions HEA2.1, HEA2.2, HEA2.3, and HEA2.4; iv) extracting fraction HEA2.5) Extraction of fraction S1, S2, S3, and S4 was performed using normal phase silica gel column chromatography and an elution solvent mixture of n-hexane:EtOAc:MeOH; v) Fraction S1 was extracted using normal phase silica gel column chromatography and an elution solvent mixture of n-hexane:EtOAc; chrysosplenol D (T1) was obtained; vi) Fraction S4 was extracted using normal phase silica gel column chromatography and an elution solvent mixture of n-hexane:EtOAc:acetone; Vitexfolin C (T2) was obtained.